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Summary of Contents for Nikon eclipse lv150
- Page 1 M350 E 05.4.NF.1 Industrial Microscope ECLIPSE LV150/LV150A Instructions...
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Page 3: Warning/Caution Symbols Used In This Manual
“ECLIPSE LV150/LV150A.” To ensure correct usage, read this manual carefully before operating the instrument. • It is prohibited to reproduce or transmit this manual in part or whole without Nikon’s expressed permission. • The contents of this manual are subject to change without notice. -
Page 4: Warning
Lamp: Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp (model LV-HL50W), or non- Nikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027) If you wish to buy these lamps, contact your nearest Nikon representative. - Page 5 WARNING 7. Light source other than the specified ones To perform the epi-fl microscopy wit the LV-UEPI2 illuminator, the specified light source brightness may be less than the desired brightness. In this case, a light source other than the specified ones, an external light source, can be used for the LV-UEPI2. Use the X-Cite 120 (manual type) or X-Cite 120PC (motorized type) manufactured by EXFO Electro-Optic Engineering Inc.
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Page 6: Caution
(flip it to the “ ” side) and unplug the power cord from the wall outlet. Then, wipe away the moisture using a dry cloth or the like. If water gets inside the microscope, do not use it; instead, notify your nearest Nikon representative. 3. Weak electromagnetic waves This microscope emits weak electromagnetic waves. - Page 7 CAUTION 6. Cautions on assembling the microscope • Be careful not to pinch your fingers or hands during assembly. • Scratches or fingerprints on the lens surface will adversely affect the microscope image. Be careful not to scratch or touch the lens surfaces. 7.
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Page 8: Layout Diagrams
LAYOUT DIAGRAMS Center of gravity position 6 x 6 STAGE JAPAN (Stage area) 570.4 Eye point 192.6 Center of gravity position Center of gravity position Dimensions are in mm. This illustration depicts the LV150A microscope configured with the LV-UEPI illuminator, LV- TI3 eyepiece tube, LV-LH50PC lamphouse, and 6x6 stage. -
Page 9: Operating Posture
OPERATING POSTURE The figure below shows the operating posture that prevents strain on your body. Choose a workbench and a chair having similar dimensions to those shown in the figure. The 95th percentile male (Height: 189.5 cm) Dimensions are in mm. * The height of the eye point is that when one eye-level riser is mounted on the microscope. -
Page 10: Table Of Contents
CONTENTS Warning/Caution Symbols Used in This Manual ..........1 Meaning of Symbols Used on the Equipment ..........2 WARNING ......................2 CAUTION ......................4 LAYOUT DIAGRAMS ................... 6 OPERATING POSTURE..................7 Names of Each Part ................... 10 1 When Configured with the Illuminator LV-UEPI ............10 2 When Configured with the Illuminator LV-UEPI2 ............ - Page 11 Assembly ....................45 1 Attaching the Stage and the Holder ................. 47 2 Assembling the Nosepiece ....................48 3 Attaching the Illuminator ....................50 4 Attaching the Lamphouse and Replacing the Lamp ............53 5 Attaching the Fiver Adapter and External Light Source ..........54 6 Attaching the Eyepiece Tube ...................
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Page 12: Names Of Each Part
Names of Each Part When Configured with the Illuminator LV-UEPI Names of Parts Vertical tube Binocular part Lamphouse OU T 10 0 Eyepiece tube LV-TI3 10 0 A . S T O F . S T O Filter sliders Illuminator LV-UEPI “CAUTION for heat”... - Page 13 I. Names of Each Part Names of Operational Parts Optical path selection lever Clamp screw for various adapters Field diaphragm open/close lever Aperture diaphragm Diopter adjustment ring open/close lever Eyepiece Analyzer slider OU T 10 0 10 0 A . S T O Prism F .
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Page 14: When Configured With The Illuminator Lv-Uepi2
When Configured with the Illuminator LV-UEPI2 Names of Parts Vertical tube Lamphouse Eyepiece tube LV-TT2 Binocular part A . S T O F . S T O Illuminator LV-UEPI2 Filter sliders “CAUTION for heat” symbol Microscopy selection knob Polarizer slider Dummy slider Ultraviolet light shield Analyzer slider... - Page 15 I. Names of Each Part Names of Operational Parts Clamp screw for various adapters Optical path selection lever Field diaphragm open/close lever Aperture diaphragm open/close lever Diopter adjustment ring Eyepiece A . S T O F . S T O Microscopy selection knob Prism Filter sliders...
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Page 16: Rear View
I. Names of Each Part Rear View “CAUTION for heat” symbol Caution label CAUTION ! - High Temperature - Do not touch the lamphouse while the lamp is lit. The surface of the lamphouse becomes hot when the lamp is on. Turn off the power and allow the lamp and lamp- house to cool enough before replacing the lamp. -
Page 17: Microscopy
Microscopy This chapter describes the procedures for each microscopy. This microscope can be configured with two types of illuminators, LV-UEPI or LV-UEPI2. See the table below for the microscopies available with each illuminator, as well as the optional accessories required for each microscopy. ●... -
Page 18: Bright-Field Microscopy
Bright-Field Microscopy When configured with the LV-UEPI Turn on the power. Push in. Raise the levers. Binocular eyepiece: 100% (P.29) To fully open the field and Set the microscope for aperture diaphragms. bright-field microscopy (p.31 and p.32) If accessories for DIC Push in the microscopy (*1 to *3) NCB11 filter. - Page 19 II. Microscopy When configured with the LV-UEPI2 Turn on the power. Raise the levers. Push in. Binocular eyepiece: 100% (P.29) To fully open the field and Set the microscope for aperture diaphragms. bright-field microscopy. (p.31 and p.32) If accessories for DIC or Push in the polarization microscopy NCB11 filter.
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Page 20: Dark-Field Microscopy
Dark-Field Microscopy When configured with the LV-UEPI Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece. (p.56 and 49) The standard sextuple nosepiece cannot be used for dark-field microscopy. Focus on the sample with bright-field microscopy. (p.16) Set the microscope for dark-field microscopy. - Page 21 II. Microscopy When configured with the LV-UEPI2 Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece. (p.56 and 49) The standard sextuple nosepiece cannot be used for dark-field microscopy. Focus on the sample with bright-field microscopy. (p.17) Set the microscope for dark-field microscopy.
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Page 22: Differential Interference Contrast (Dic) Microscopy
Differential Interference Contrast (DIC) Microscopy When configured with the LV-UEPI Mount objectives marked “LU”, universal quintuple nosepiece or motorized universal quintuple nosepiece, polarizer, analyzer, and DIC slider. (p.36, 38, 39, 49, and 56) Focus on the sample with bright-field microscopy. (p.16) Set the microscope for DIC microscopy. - Page 23 II. Microscopy When configured with the LV-UEPI2 Mount objectives marked “LU”, universal quintuple nosepiece, polarizer or motorized quintuple nosepiece, polarizer, analyzer, and DIC slider. (p.36, 38, 39, 49, and 56) Focus on the sample with bright-field microscopy. (p.17) Set the microscope for DIC microscopy. Select “A”...
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Page 24: Simplified Polarization Microscopy
Simplified Polarization Microscopy When configured with the LV-UEPI Mount a polarizer and an analyzer. (p.36, 38) Focus on the sample with bright-field microscopy. (p.16) Set the microscope for simplified polarization microscopy. Push in. Analyzer (P.38) A ST O F. ST O Adjust the Push in and align brightness. - Page 25 II. Microscopy When configured with the LV-UEPI2 Mount a polarizer and an analyzer. (p.36, 38) Focus on the sample with bright-field microscopy. (p.17) Set the microscope for simplified polarization microscopy. Push in. Analyzer (p.38) A. ST O F. ST O Adjust the brightness.
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Page 26: Sensitive Polarization Microscopy
Sensitive Polarization Microscopy Only when configured with the LV-UEPI2 Mount a polarizer, lambda plate, and analyzer. (p.36 to 38) Focus on the sample with bright-field microscopy. (p.17) Set the microscope for sensitivity polarization microscopy. Push in. Analyzer (p.38) A. ST O F. -
Page 27: Epi-Fluorescence Microscopy
II. Microscopy Epi-Fluorescence Microscopy Only when configured with the LV-UEPI2 Attach the filter cube to the turret in the illuminator. (p.52) Up to two filter cubes can be attached. Install the suitable illuminator for the excitation method as necessary. (p.54) To perform the epi-fl microscopy, the brightness of the specified light source (halogen lamp) may be less than the desired brightness. -
Page 28: Operation Of Each Part
Operation of Each Part Operation of the Illumination Brightness control Power When the specified lamphouse LV-LH50PC is used for the indicator halogen lamp to illuminate, the brightness can be controlled by rotating the brightness control knob. * When an external light source is used, the brightness is controlled by the external light source or the ND filters OB J. -
Page 29: Coarse/Fine Focus Knobs
III. Operation of Each Part Coarse/Fine Focus Knobs Relationship between focus knob rotation and stage vertical movement The relationship between the direction of coarse/fine focus knob rotation and the stage vertical movement is shown in the figure. • The stage moves approximately 14.0 mm per one A ST OP F. - Page 30 Coarse focus stopper The coarse focus stopper restricts the movement of the coarse focus knob so that the stage cannot be raised higher than the position the operator specifies. When the coarse focus stopper ring is rotated in the direction of the arrow (labeled “CLAMP→”) on the microscope base, the coarse focus knob cannot be used to move the stage any higher.
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Page 31: Eyepiece Tube
III. Operation of Each Part Eyepiece Tube Optical path selection The optical path selection lever can be used to switch between the proportions of light reaching the binocular part and the vertical tube. Light proportion Light proportion Lever Lever position position Binocular part Vertical tube... -
Page 32: Diopter Adjustment
Diopter Adjustment Diopter adjustment compensates for differences in eyesight between your left and right eyes. After the correct adjustment, you will find the observation with both eyes easier and the focus shift is reduced when switched to different objectives. Be sure to adjust the diopter adjustment rings on both eyepieces. -
Page 33: Field Diaphragm
III. Operation of Each Part Field Diaphragm The field diaphragm open/close lever changes the size of the field Image of field diaphragm diaphragm. Adjust the size of the diaphragm until it circumscribes or inscribes the viewfield. About the field diaphragm •... -
Page 34: Aperture Diaphragm
Aperture Diaphragm Remove one of the eyepieces. Operating the aperture diaphragm Objective’s exit pupil open/close lever will change the size of the aperture diaphragm as seen within the objective's exit pupil in the eyepiece tube. Generally, the aperture diaphragm should be adjusted to about 70 to 70 to 80 80% of the numerical aperture of the objective. -
Page 35: Illumination Selection Lever And Microcopy Selection Knob
III. Operation of Each Part Illumination Selection Lever and Microcopy Selection Knob 1. Illumination selection lever (for the LV-UEPI) When the illuminator LV-UEPI is used, the illumination selection lever on the right side can be used to alternate the microscopy illumination between bright-field (BF) and dark- field (DF). -
Page 36: Stage
Stage 1. 6x6 stage The stage can be moved in either the “coarse” mode for swift and long ranged movement, or the “fine” mode for minute movement. To switch between the modes, use the stage coarse/fine movement selection switch on the right side of the stage’s top plate. The “coarse”... -
Page 37: Motorized Nosepiece Operation
III. Operation of Each Part Motorized Nosepiece Operation The LV150A is equipped with a motorized nosepiece, and you can rotate the nosepiece by operating the nosepiece rotation buttons on the left side of the microscope to change objectives. Two buttons, far side and near side, are used as the nosepiece rotation buttons. -
Page 38: Polarizer Slider
Polarizer Slider The polarizer slider can be used together with the First clickstop position analyzer slider to enable the simplified polarization Second clickstop position microscopy. Likewise, the polarizer slider can be Polarizer rotating ring combined with the analyzer slider and DIC slider to perform DIC microscopy, and with the analyzer slider and lambda plate slider to perform sensitive polarization microscopy (with the LV-UEPI2 only). -
Page 39: Lambda Plate Slider (For The Lv-Uepi2 Only)
III. Operation of Each Part UV polarizer slider The UV polarizer slider is used for the epi-microscopy under the UV excitation light to make the excitation light to the linear polarization. The UV polarizer will deteriorate over time, so change it as necessary. -
Page 40: Analyzer Slider
Analyzer Slider The analyzer slider can be used together with the polarizer First clickstop position slider to enable simplified polarization microscopy. Second clickstop position Likewise, the analyzer slider can be combined with the polarizer slider and DIC slider to perform DIC microscopy, and with the polarizer slider and lambda plate slider to perform sensitive polarization microscopy (with the LV- UEPI2 only). -
Page 41: Dic Slider
III. Operation of Each Part DIC Slider For DIC microscopy, use the DIC slider together with Prism selection knob the polarizer and analyzer sliders. Prism movement knob Sliding limit groove Attaching/removing the DIC slider Use a hexagonal screwdriver to loosen the DIC slider limit screw on the nosepiece. -
Page 42: Filter Cubes For Fluorescence Observation (For The Lv-Uepi2 Only)
* Please take note that if a light source other than the specified ones are installed onto this microscope, this microscope system will not be treated as a TUV/SEMI approved product. Nikon recommends that the light source to be installed onto this microscope should have been tested by a safety certification organization. - Page 43 III. Operation of Each Part Selecting a barrier (BA) filter The barrier filter transmits only the fluorescence emitted by the sample, blocking the excitation light. This enables observation of fluorescence images having less unnecessary light (darker background). BA filters are available in two types: long-pass (LP) and band-pass (BP). The LP filter blocks all the light of shorter wavelength than a given value.
- Page 44 Replacing the excitation filter, barrier filter, and dichroic mirror The excitation filter, barrier filter, and dichroic mirror can be removed from the filter cube and replaced with different parts. When handling these parts, put on gloves and do not touch the surface of filters and mirrors with bare hands.
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Page 45: Excitation Light Balancer (For The Lv-Uepi2 Only)
III. Operation of Each Part Excitation Light Balancer (for the LV-UEPI2 Only) When the illuminator LV-UEPI2 is used, the optional D- FB excitation light balancer can be attached for the epi-fl microscopy to observe specimens stained in multiple colors. D -F B The excitation light balancer enables the continuous change of the wavelength characteristics for the Excitation light balancer... - Page 46 III. Operation of Each Part Detailed specification of excitation light balancer : Effective diameter on the aperture diaphragm surface Transmittance 100% TRITC FITC DAPI Texas-Red Wavelength The transmittance for the FITC is designed to keep approximately 100%, because the FITC is usually dark fluorescent image.
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Page 47: Assembly
Nikon LV-LH50PC precentered lamphouse 12V 50W • Lamphouse Nikon LV-LH50PC precentered lamphouse 12V 50W • Lamp Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp or non-Nikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027) - Page 48 Assembling the ECLIPSE LV150/LV150A • When using the illuminator LV-UEPI Dummy LV-TI3 slider eyepiece tube Eyepiece Filter sliders Analyzer slider (x2) Various filters Dummy slider Analyzer slider Lamphouse 12V 50W LV-UEPI illuminator halogen lamp BD quintuple Standard nosepiece sextuple Lamp...
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Page 49: Attaching The Stage And The Holder
IV. Assembly Attaching the Stage and the Holder 1. Attaching the sub-stage The sub-stage must be attached onto the microscope before installing the stage. 1 Lower the sub-stage completely with the coarse focus knob. Sub-stage 2 Loosen the clamp screw for the sub-stage. And then attach the sub-stage with fitting the dovetail joints on the up/down part. -
Page 50: Assembling The Nosepiece
3. Attaching the Stage and the Holder 6x6 stage 1 Lower the sub-stage completely with the coarse focus knob. 2 Remove the fixing metals from the stage plate by using the 3-mm hexagonal wrench to the four hex screws. 3 Place the stage on the substage and fix it with the four M4 screws that were attached to the sub-stage. - Page 51 IV. Assembly 2. Assembling the motorized nosepiece For the LV150A, the motorized nosepiece must be attached. The motorized nosepiece should be assembled before attaching the illuminator. 1 Remove the cover from the connection block by unscrewing the two M4 screws on the top of the microscope arm.
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Page 52: Attaching The Illuminator
Attaching the Illuminator 1. Illuminator main unit 1 Loosen sufficiently the illuminator clamp screw on the front of the microscope arm using the hexagonal screwdriver. 2 Mount the illuminator onto the microscope arm and fix it by tightening the illuminator clamp screw. - Page 53 IV. Assembly 2. Sliders (dummy sliders, polarizer slider, lambda plate slider, and analyzer slider) • For the LV-UEPI: The sliders are to be inserted into the slots on the front and the right side of the illuminator. In case of dummy sliders, slide them in till the limit (so that the empty hole will be set in the optical path). •...
- Page 54 4. Filter cubes for fluorescence observation (for the LV-UEPI2 only) The LV-UEPI2 accommodates two filter cubes for epi-fluorescence microscopy. 1 Verify that the illumination shutter is closed and the power supplies to the microscope and light source are off. 2 Remove the cover from the left side of the illuminator.
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Page 55: Attaching The Lamphouse And Replacing The Lamp
(for at least 30 minutes after the lamp is turned off), before replacing the lamp. • Use the Nikon LV-LH50PC halogen lamphouse for the lamphouse. • Use the Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp or non-Nikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027) for the lamp. -
Page 56: Attaching The Fiver Adapter And External Light Source
2. Replacing the lamp The lamp can be removed without having to detach the lamphouse from the microscope. Before performing the following procedures, turn off the power supply for the microscope (press the “ ” side) and unplug the power cable from the wall outlet. And check that the lamp and lamphouse have cooled down sufficiently. - Page 57 IV. Assembly CAUTION • If a light source other than the specified ones are installed onto this microscope, this microscope system will not be treated as a TUV/SEMI approved product. • Carefully read the instruction manual for an external light source to use it, and follow their instructions.
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Page 58: Attaching The Eyepiece Tube
Attaching the Eyepiece Tube Fully loosen the eyepiece tube clamp screw with the hexagonal screwdriver. Fit the eyepiece tube onto the mount on the top of the illuminator and tighten the eyepiece tube clamp screw with the hexagonal screwdriver. When using the LV-UEPI When using the LV-UEPI2 Eyepiece tube Eyepiece tube... -
Page 59: Attaching Eye Level Riser
IV. Assembly Attaching Eye Level Riser The optional eye level riser is used for the adjustment of the height of the eyepiece tube to fit the observer’s eye point. Up to two eye level risers can be attached in piles. When one eye level riser is attached, the eyepiece height rises 25 mm. -
Page 60: Connecting The Power Cord
Connecting the Power Cord WARNING Use only the supplied power cord. Using the wrong power cord could cause hazards or fire. Also, connect the microscope to a PE (protective earth) terminal, since the microscope complies with the electric shock to protection class I. For details about the power cord, see “VIII. -
Page 61: Countermeasures For Earthquakes
4. Assembly Countermeasures for Earthquakes To prevent the microscope from being slipped and shaken by the strong shake of an earthquake, we recommend taking the following countermeasures: Prepare four angle-shaped brackets as shown in the figure, and screw them onto the table so that the microscope is held tight by the brackets. -
Page 62: External Communications Control
External Communications Control The LV150A has an RS-232C interface. The serial communication can be used to rotate the nosepiece and read its position or to set and read status by external device, such as PC. 1. Communication Method Asynchronous (start-stop synchronized) serial communication RS-232C (EIA standard compliant) 2. - Page 63 V. External Communications Control 3. Cable Specifications The following diagram shows signal connections necessary for a cable to work with the factory default. For 9-pin connector type external device For 25-pin connector type external device External device External device This system This system (ex.
- Page 64 (2) Sending Format: [Identification Code] [Command] [Data] [<CR>] [Identification Code]: 1 lower-case alphabetic character (ASCII code, 1 byte) Identification code Specifications Acknowledging response against a “c“ code Negative acknowledging response against a “c” or “r” code Acknowledging response against an “r“ code Status transmission [Command]: 3 upper-case alphabetic characters (ASCII code, 3 bytes), [?] (ASCII code, 0x3F) [?] is added when the [command] portion of a message received by this unit is...
- Page 65 V. External Communications Control (2) When the status output of this unit is enabled; External device This unit Switch operation (manual rotation) Sending format Receiving a status Sending a status *1 Control processing Sending format Receiving a status Sending a status *2 External device This unit Receiving format...
- Page 66 7. List of Control Commands Identification Command Data Specifications code Rotates the nosepiece in forward direction to the next address. Rotates the nosepiece in reverse direction to the next address. (However, rotation from nosepiece address 1 to 5 is prohibited.) Rotates the nosepiece in reverse direction to the next address.
- Page 67 V. External Communications Control [r] [VER] [<CR>] Reads the program version number. → When properly finished [a][VER][data][<CR>] → When control error occurred [n][VER][error code][<CR>] [data]: V*.** (* denotes numeral. Example: V1.00) [r] [PNM] [<CR>] Reads the program name. You can identify devices connected on the communication line from an external device.
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Page 68: Troubleshooting
Troubleshooting Improper use of the microscope may adversely affect its performance even though there is no damage on itself. If any of the problems listed below arises, take the countermeasures indicated. Viewing and control systems Problems Cause Countermeasures The viewfield is Lamp not properly installed. - Page 69 VI. Troubleshooting Problems Cause Countermeasures Elongated image Nosepiece is not properly installed. Install it securely. (p.49) Nosepiece is not rotated to a clickstop Rotate to a clickstop position. position. Stage is slanted. Mount it securely. (p.48) Microscope is not installed on a flat Install it on a flat surface.
- Page 70 Problems Cause Countermeasures Eyepiece diopter is not adjusted. Adjust the diopter. (p.30) Objective hits the sample when switched from low to high magnification. Eyepieces are not mounted correctly. Mount them by aligning the positioning Sample is out-focused by grooves. (p.56) objective switching.
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Page 71: Electrical
VI. Troubleshooting Electrical Problems Cause Countermeasures Power is not supplied. Plug the power cord into an outlet. Lamp does not light (p.58) when switched on. Power cord is not connected to Connect the power cord. microscope. (p.58) Cable of lamphouse is not connected to Connect the cable. -
Page 72: Care And Maintenance
Care and Maintenance Nikon recommends daily care and maintenance for maintaining the performance as long as possible. Do not let dust, fingerprints, and the like, get on the lenses. Dirt on the lenses, filters, and the like will adversely affect the optical performance of the microscope. -
Page 73: Cleaning Lenses And Filters
• Before putting on the plastic cover, turn off the power switch of the microscope (flip it to the “ ” side) and wait until the lamphouse is cool. Regular Inspections Regular inspections of this microscope are recommended in order to maintain peak performance. Contact your nearest Nikon representative for details about regular inspections. -
Page 74: Specifications
Specifications Model name ECLIPSE LV150, ECLIPSE LV150A Optical system CFI60 optical system (infinity-corrected CF optical system) Illumination Epi-illumination system: The power source for the lamp, NCB11, ND4, and ND16 are built-in. (exchangeable) Specified illuminator: LV-UEPI illuminator or LV-UEPI2 illuminator Lamp ratings:... - Page 75 VIII. Specifications • This product got the TUV SEMI mark. (SEMI guideline S2-0703, S8-1108) Safety standards compliance • This is UL-listed product. (UL61010A-1) • This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.