Table of Contents

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M559 E
10.11.NF.1
*M559EN01*
P-400R Digital Microscope
Instructions

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Summary of Contents for Nikon P-400R

  • Page 1 M559 E 10.11.NF.1 *M559EN01* P-400R Digital Microscope Instructions...
  • Page 3  If you intend to use any other products with this product, read the manual of such products as well.  If you want to attach the P-400R Digital Microscope to a P-MFSC Motorized Focusing Stand Controller for use with a P-TPM Touch Panel Monitor, see the “ShuttlePix Digital Microscope Instructions”.
  • Page 4: Features Of This Product

    P-400R Digital Microscope Instructions Features of This Product The Nikon P-400R is a digital microscope provided with a 20x optical zoom lens. This product has a built-in CCD camera, and allows you to observe the image with a monitor on the side of the product and the image can be played on the LCD monitor of this system as well as an external monitor.
  • Page 5: Table Of Contents

    Observing ....................... 25 4.3.1 Starting shooting mode..............25 4.3.2 Directing the P-400R to the object ........... 26 Holding the microscope in hand ............ 26 Using the microscope with a tripod ..........26 Using the microscope with the contact observation adapter ..27 4.3.3...
  • Page 6 P-400R Digital Microscope Instructions 4.3.8 Changing the aperture mode............35 4.3.9 Adjusting the exposure ..............36 Compensating the exposure ............36 Setting the shutter speed .............. 37 Setting the camera gain ..............38 Using the AE lock ................39 4.3.10 Setting the white balance ..............
  • Page 7 10.3 Cleaning Painted, Printed, or Plastic Parts............. 93 10.4 Storage ......................93 10.5 Periodic Inspections (Charged) ..............93 11 Major Specifications ..................94 11.1 P-400R Digital Microscope ................94 11.2 AC Adapter ..................... 98 11.3 P-RELS Release .................... 98 11.4 Supported Standards..................99...
  • Page 8: Safety Precautions (Be Sure To Read The Following Before Use)

    P-400R Digital Microscope Instructions Safety Precautions (Be sure to read the following before use) For safe and proper use of this product, carefully read the following precautions. WARNING and CAUTION symbols used in this manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage.
  • Page 9 Use the specified AC adapter. An optional AC adapter can be used as a power supply for the P-400R. Be sure to connect the specified AC adapter to the microscope’s main unit. If any other AC adapter is connected, it may cause malfunction or overheating of this product or fire.
  • Page 10 Use the specified power cords. Make sure that the power cord connected to the P-400R AC adapter is the power cord specific to the P-400R Digital Microscope. If any other power cords are used, it may cause malfunction or overheating of the equipment or fire.
  • Page 11 Never use straps designed for mobile phones.  When you carry the P-400R with the strap attached, be careful not to let the strap to get caught on something. Otherwise, it may cause...
  • Page 12 P-400R Digital Microscope Instructions CAUTION (for the overall product) Precautions for usage environment and storage environment This product is a precision optical device. Using or storing it under improper environment may result in a failure or degraded precision. When using or storing this product, observe the following conditions.
  • Page 13 Otherwise, the battery may leak, explode, or overheat. This could result in burning, personal injury, or fire. Do not use the battery for any other equipment than the P-400R Digital Microscope. Doing so may cause the battery to leak, explode, or overheat. This could result in burning, personal injury, or fire.
  • Page 14 Otherwise, the battery may leak, explode, or overheat. This could result in burning, personal injury, or fire. If you notice any abnormality of the battery, contact your nearest Nikon representative. When you recycle or discard the battery, insulates the battery’s contacts with tape, etc.
  • Page 15 P-400R Digital Microscope Instructions CAUTION (for the battery) If this product is not used for a long time, remove the battery. Otherwise, the battery may leak, explode, or overheat, which may cause malfunction of this product, personal injury, burning, or fire.
  • Page 16 Otherwise, the battery charger may ignite, leading to fire. Be careful not to burn yourself when you pull out the battery charger’s power cord from the outlet. Ask your nearest Nikon representative for repair. Do not allow the battery charger to become wet.
  • Page 17: Names Of Components

    This chapter describes the names of major components of the P-400R Digital Microscope. Before using P-400R for the first time, read this chapter to confirm the names of individual components and their positions. Return to this chapter whenever you want to confirm the names and positions of components.
  • Page 18 Strap usage example Caution: About attaching a strap  The P-400R weighs approximately 1.1 kg including the battery. Make sure that you use a camera strap or a similar strap that has adequate strength.  Do not use a thin strap like the one for a mobile phone. Such a strap might be...
  • Page 19 P-400R Digital Microscope Instructions Information displayed in the monitor Shooting mode Items displayed during capturing are listed below. Table: Display in the shooting mode Item Content : Standard, : Wafer/IC chip, : Metal Ceramic, Scene mode : Circuit board, : FPD (Flat Panel Display),...
  • Page 20 P-400R Digital Microscope Instructions Playback mode Items displayed during playback are listed below. Table: Display in the playback mode Item Content Captured date and time Date and time that the image was captured File name Name of the file Remaining battery level...
  • Page 21 P-400R Digital Microscope Instructions Menu screen P-400R has three menus, [Shooting menu], [Playback menu] and [Setup menu], for performing various settings. When you press the [MENU] button in shooting mode, the [Shooting menu] appears and in playback mode, the [Playback menu] appears. To display the [Setup menu] screen, select the [Setup menu] tab ( ) using the multi-selector in the shooting menu or playback menu.
  • Page 22: Preparing For Use

    P-400R Digital Microscope Instructions Preparing for Use Preparing a Battery The P-400R is a battery-powered digital microscope, which allows you to observe and capture specimens or play back recorded images without any cable connections. 2.1.1 Charging a battery Use the dedicated P-CH01 P-BACH Battery Charger to charge a P-Li01 P-Li-ion Rechargeable Battery.
  • Page 23 P-400R Digital Microscope Instructions CHARGE lamp status The following table describes the statuses of the CHARGE lamp of the P-CH01 P-BACH Battery Charger. Table: CHARGE lamp statuses Status Meaning Description The battery is now being charged. Now charging Charging Charging of the battery is completed.
  • Page 24: Inserting A Battery

    P-400R Digital Microscope Instructions 2.1.2 Inserting a battery Insert a P-Li01 P-Li-ion Rechargeable Battery into the P-400R. Open the battery cover at the bottom of the grip. Slide the battery chamber Battery open/close knob in the orientation chamber as indicated in the illustration (1) to open/close open the battery cover (2).
  • Page 25 P-400R Digital Microscope Instructions Removing the battery If a battery to be charged is in the P-400R Digital Microscope, remove the battery from the microscope. Turn off the power of the P-400R. Check that the power indicator is off. Open the battery cover at the bottom of the grip.
  • Page 26: Precautions For The Battery And Battery Charger

    In such a case, remove the battery, attach it again, and then restart charging.  If an abnormal condition or a problem occurs, immediately stop the use, and ask your nearest Nikon representative for repair.
  • Page 27: Preparing The Recording Medium

    A miniSD card and a micro SD card cannot be used via a commercially available conversion adapter in P-400R. Use a full-size SD card. About handling an SD card Note the following when you use an SD card: ...
  • Page 28: Preparing The Sd Card

    Pay attention to the inserting direction of the SD card The SD card cannot be inserted in the opposite orientation. If you insert it forcibly in the incorrect orientation, there is a risk to damage the SD card or the P-400R.
  • Page 29: Formatting The Sd Card

    About initializing the SD card  Before using an SD card, which was used with any other device, on P-400R, be sure to format it in P-400R.  After formatting, the data stored in the SD card will be all deleted. If some important data is contained in the SD card, take the backup to the personal computer, etc.
  • Page 30: Specifying An Active Folder

    2.2.2 Specifying an Active Folder When you initialize an SD card in P-400R, a sub folder “100NIKON” is created in the “DCIM” folder in the SD card, and it is automatically set as an image recording destination (“active folder”).
  • Page 31: Connecting An External Monitor (Optional)

    P-400R Digital Microscope Instructions Connecting an External Monitor (optional) The optional P-CV Video Cable can be used to connect the P-400R to an external monitor such as a TV set so that the live microscopic image or captured microscopic images can be displayed in the external monitor.
  • Page 32: Connecting The Ac Adapter (Optional)

    AC adapter when the P-400R power is on, the P-400R or data might be damaged.  The battery in the P-400R is not charged when the AC adapter is connected to the P-400R. If you want to charge the battery that is in the P-400R, remove the battery from the P-400R, and then charge the battery with the P-CH01 P-BACH battery charger.
  • Page 33: Setting The Display Language And The Date And Time (At Initial Power-On)

    P-400R Digital Microscope Instructions Setting the Display Language and the Date and Time (At Initial Power-On) When the power is turned on for the first time after the purchase, the [Language] menu is displayed on the LCD monitor. Make settings according to the following procedure.
  • Page 34: Basic Operation

    P-400R Digital Microscope Instructions Basic Operation This chapter explains you how to capture and play back images using the P-400R. For the details This chapter explains only simple operational procedures. For details, see “4 Basic Operations for Capturing and Playing Back”.
  • Page 35: Obtaining Optimal Shooting Conditions

    P-400R Digital Microscope Instructions Obtaining optimal shooting conditions Check that [STD] is displayed on the top left of the LCD monitor. When the scene mode is standard, [STD] is displayed. For the general objects, you can use this mode for capturing.
  • Page 36: Playing Back The Captured Image

    The last captured image is displayed. To switch the displayed playback Displays image, press the right and left (or top and bottom) buttons of the previous image multi-selector. LCD panel When you press the capture Displays the switch, the P-400R enters shooting next image mode.
  • Page 37: Deleting Captured Image

    P-400R Digital Microscope Instructions Deleting captured image Play back the image to be deleted and press the delete button ( ). A confirmation message asking you LCD panel whether to delete the image Delete button appears. When you press the delete button...
  • Page 38: Basic Operations For Capturing And Playing Back

    Turning off the power Pressing the power button when the P-400R power is on, the power is turned off. The LCD monitor and ring illuminator, and the power switch indicator are turned off.
  • Page 39 P-400R Digital Microscope Instructions Checking the remaining battery charge level Remaining battery charge level When the power is turned on, the remaining battery charge level might be displayed at the top right of the LCD monitor. The remaining battery charge level is displayed when the level is low.
  • Page 40: Operating The Lcd Monitor

    P-400R Digital Microscope Instructions Operating the LCD Monitor 4.2.1 Opening the LCD panel When capturing or playing back images, 90º open the LCD panel, and adjust its angle so 180º that you can view the monitor easily. The LCD panel can rotate 180º horizontally and 90º...
  • Page 41: Observing

    Live image and grid lines Displaying the shooting menu When the P-400R is in shooting mode, if you press the [MENU] button, the shooting menu is displayed. For details about how to use the shooting menu, see “5 Advanced Use of Capturing Functions”.
  • Page 42: Directing The P-400R To The Object

    4.3.2 Directing the P-400R to the object The focus position of the P-400R is 29 mm from the front surface of the adapter. Remove the lens cover, direct the P-400R lens to the specimen, and move the lens toward the specimen at about 29 mm off the specimen.
  • Page 43: Using The Microscope With The Contact Observation Adapter

    P-400R Digital Microscope Instructions Using the microscope with the contact observation adapter When the contact observation adapter that came with the P-400R is attached, Contact observation the distance between the front surface of adapter the adapter and the lens is approx. 29...
  • Page 44: Operating The Illumination

    P-400R Digital Microscope Instructions 4.3.3 Operating the illumination The P-400R has a ring illuminator consisting of eight white LEDs around the objective. Caution: Notes on the ring illuminator Note the following when using the illuminator:  Do not point the luminous section to a person.
  • Page 45: Adjusting Brightness Of The Illumination

    P-400R Digital Microscope Instructions Adjusting brightness of the illumination Adjust brightness of the illumination as described below. Press ([+]/[-]) of the light control button ) to adjust brightness. When you press the light control button, the [Light value] menu is displayed. In this...
  • Page 46: Using Zoom

    P-400R Digital Microscope Instructions 4.3.4 Using zoom This product adopts a 20x optical zoom lens allowing 16 levels of zoom magnifications which can be set from the LCD panel. Direct the lens to the object, and check it with the LCD monitor.
  • Page 47: Checking The Focus

    - Object with regular vertical lines About the P-400R focus position The P-400R focus position is 29 mm off the end of the objective. Adjust the position of the P-400R so that the object is 29 mm off the objective.
  • Page 48: Selecting A Scene Mode Suitable To The Specimen

    P-400R Digital Microscope Instructions 4.3.6 Selecting a scene mode suitable to the specimen Several scene modes are provided according to typical specimens. Selecting a scene mode can use the setting suitable to the specimen. Make sure that the product is in shooting mode.
  • Page 49 P-400R Digital Microscope Instructions About settings at the power-on time The selection of scene mode is saved when the power is turned off. Next time the power is turned on, the previously selected scene mode is selected again. About changing the scene mode setting ...
  • Page 50: Using Custom Settings

    P-400R Digital Microscope Instructions 4.3.7 Using custom settings You can register a maximum of four kinds of shooting conditions as “custom settings”. One of four kinds of settings registered as Custom 1 to Custom 4 can be reproduced by selecting Custom 1 to Custom 4 for the scene mode.
  • Page 51: Changing The Aperture Mode

    P-400R Digital Microscope Instructions 4.3.8 Changing the aperture mode You can select either [Resolution-priority] or [DOF-priority] as the aperture mode for shooting. Make sure that the product is in shooting mode. Press the top button ( ) of the multi-selector to display the scene LCD panel mode selection menu.
  • Page 52: Adjusting The Exposure

    When the exposure mode is Program AE or Shutter-priority AE, you can adjust the exposure by exposure compensation. Exposure compensation is a function that allows you to adjust the brightness of the screen by manually changing the exposure automatically calculated by the P-400R. Check that the exposure mode is P: Program AE or S: Shutter-priority AE Program AE or Shutter-priority AE.
  • Page 53: Setting The Shutter Speed

    P-400R Digital Microscope Instructions Setting the shutter speed When the exposure mode is Shutter-priority AE or Manual, you can set the shutter speed arbitrarily. Check that the exposure mode is set to S: Shutter-priority AE or M: Manual Shutter-priority AE or Manual.
  • Page 54: Setting The Camera Gain

    The camera gain is used to set the sensitivity of the P-400R. When a higher value is set for the camera gain, darker objects can be captured. However, higher sensitivity is likely to cause more noises in the image.
  • Page 55: Using The Ae Lock

    P-400R Digital Microscope Instructions Using the AE lock When the exposure mode is Program AE or Shutter-priority AE, it is possible to adjust and lock the exposure for a particular part of the object. Use this function in such a case where the brightness of the part for which the exposure is to be adjusted is significantly different from the other parts.
  • Page 56: Setting The White Balance

    White balance is the process of correcting color bias depending on the light source and adjusting the color of white so that white objects appear really white in the image. The P-400R allows you to adjust the white balance manually. Prepare a sheet of white paper or a...
  • Page 57: Capturing The Image Under Observation

    P-400R Digital Microscope Instructions Capturing the Image under Observation Checking the active folder Microscopic images are saved in the preset active folder. If necessary, check the active folder settings by referring to “4.2 Operating the LCD Monitor”. 4.4.1 Capturing an image To capture an image, press the capture switch in shooting mode.
  • Page 58 For switching continuous shooting mode, see “5.10 Using Continuous Shooting Mode”. Using the release switch Even when the P-400R has been attached to a tripod, vibration might occur when the capture switch is pressed. In such a case, using the optional P-RELS Release is recommended.
  • Page 59: Playing Back Captured Images

    Displaying the playback menu When the P-400R is in playback mode, if you press the [MENU] button, the playback menu is displayed. For details about how to use the playback menu, see “6 Advanced Use of Playback Functions”.
  • Page 60: Switching The Image

    4.5.3 Displaying thumbnail images If you press the [-] side of the zoom button ( when the P-400R is in playback mode, the screen changes to the thumbnail view in which nine thumbnail images are displayed. LCD panel ...
  • Page 61: Enlarging An Image

    P-400R Digital Microscope Instructions 4.5.4 Enlarging an image When the P-400R is in playback mode, if you press the [+] side of the zoom button ( ), the displayed image is magnified.  Normally, the image is displayed at LCD panel magnification of 100%.
  • Page 62: Deleting Captured Images

    P-400R Digital Microscope Instructions Deleting Captured Images 4.6.1 Deleting the image that has just been captured To delete the last image captured in shooting mode, perform the following procedure. In shooting mode, press the delete button ( ). The last saved image and a message...
  • Page 63: Deleting The Currently Displayed Image

    P-400R Digital Microscope Instructions 4.6.2 Deleting the currently displayed image To delete an image in playback mode, perform the following procedure. In playback mode, display the image you want to delete. Press the delete button ( ). A message asking you whether you really want to delete the image is displayed.
  • Page 64: Advanced Use Of Capturing Functions

    Multi-selector Restarting shooting mode If the [MENU] button or the capture switch is pressed when the shooting menu is displayed, the P-400R is placed in shooting mode again, and the live image is displayed in the LCD monitor.
  • Page 65 P-400R Digital Microscope Instructions Operations that can be performed using the shooting menu Table: Operations that can be performed using the shooting menu Section Item Description Page A maximum four sets of shooting Registering a Custom conditions can be registered as custom Definition definitions.
  • Page 66: Registering A Custom Definition

    P-400R Digital Microscope Instructions Registering a Custom Definition The current shooting conditions can be registered as a custom definition. You can register a maximum of four custom definitions. The procedure is as follows. Set desired shooting conditions. Set shooting conditions that you want to use as a set of custom settings. For details about how to set shooting conditions, see “4 Basic Operations for...
  • Page 67: Setting The Active Folder

    P-400R Digital Microscope Instructions Setting the Active Folder Specify the folder in which images will be saved. Location and name of the active folder The active folder is created in the DCIM folder on the SD card. The format of the folder name is nnnNIKON (nnn: three-digit number).
  • Page 68: Setting An Existing Folder As The Active Folder

    P-400R Digital Microscope Instructions 5.2.2 Setting an existing folder as the active folder You can set an existing folder as the active folder. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen.
  • Page 69: Selecting The Image Quality Mode

    P-400R Digital Microscope Instructions Selecting the Image Quality Mode You can select the image quality mode used when recording images. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen. Select [Image quality], and then press the [OK] (or right) button.
  • Page 70: Selecting The Image Size

    P-400R Digital Microscope Instructions Selecting the Image Size You can set the size of images to be recorded. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen. Select [Image size], and then press the [OK] (or right) button.
  • Page 71: Selecting The Exposure Mode

    The [Exposure mode] screen appears. Select one of the following three exposure modes:  Prog. AE (initial setting) The P-400R automatically sets both the shutter speed and the camera gain.  Shutter-priority AE The P-400R automatically sets the camera gain according to the shutter speed set by the user.
  • Page 72: Program Ae Settings

    P-400R Digital Microscope Instructions Program AE Settings In Program AE mode, you can set the upper limit on the camera gain and the lower limit on the shutter speed. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen.
  • Page 73 If the brightness of the object is low, the P-400R sets the shutter speed to the lower limit, and adjusts the camera gain to obtain the correct exposure. If the object is underexposed even when the camera gain is set to the upper limit, a shutter speed slower than the lower limit is used.
  • Page 74: Setting The Quality Of Images To Be Captured

    P-400R Digital Microscope Instructions Setting the Quality of Images to be Captured You can set the quality of images to be captured on a fine-grained basis. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen.
  • Page 75 P-400R Digital Microscope Instructions  Hue A value from -50 to +50 can be set. The value can be incremented or decremented by 1. The initial value is 0.  Contrast One of the following seven preset contrast settings can be selected.
  • Page 76 P-400R Digital Microscope Instructions About contrast setting The following seven preset items are provided for the contrast setting. Select the preferable setting depending on the object.  1: Wide D-range Use this for the high-luminance object observation which requires the gradation setting from dark to bright.
  • Page 77: Switching The Metering Method

    P-400R Digital Microscope Instructions Switching the Metering Method You can set the metering method used for observation and capturing. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen. Select [Metering method], and then press the [OK] (or right) button.
  • Page 78: Switching The Metering Area

    P-400R Digital Microscope Instructions Switching the Metering Area You can set the size of the metering area. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen. Select [Metering area], and then press the [OK] (or right) button.
  • Page 79: Using Continuous Shooting Mode

    P-400R Digital Microscope Instructions 5.10 Using Continuous Shooting Mode You can specify the continuous shooting mode settings to be used during capturing. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen.
  • Page 80 P-400R Digital Microscope Instructions  Self-timer In this mode, capturing starts two seconds after the capture switch is pressed. When the capture switch is pressed, the counter starts counting down. When the counter value is 0, capturing is performed. If this mode is selected, the timer icon ( ) is displayed in the LCD monitor.
  • Page 81: Using Shading Correction

    P-400R Digital Microscope Instructions 5.11 Using Shading Correction Shading correction is functionality that corrects uneven exposure that is due to, for example, limb darkening. You can select whether to enable this functionality. The procedure is as follows. Select [Shading correction], and then press the [OK] (or right) button.
  • Page 82: Setting The Scale

    P-400R Digital Microscope Instructions 5.12 Setting the Scale You can specify the settings for the scale displayed during observation and capturing. The procedure is as follows. In shooting mode, press the [MENU] button to display the [Shooting menu] screen. Select [Scale configuration], and then press the [OK] (or right) button.
  • Page 83: Advanced Use Of Playback Functions

    Pressing the left button redisplays the upper-level menu item. Multi-selector Restarting playback mode If the [MENU] or playback button is pressed when the playback menu is displayed, the P-400R is placed in playback mode again, and a saved image is displayed in the LCD monitor.
  • Page 84 P-400R Digital Microscope Instructions Operations that can be performed using the playback menu Table: Operations that can be performed using the playback menu Section Item Description Page The images selected by using their Deleting Multiple thumbnail images or a folder containing Images All at One Time images can be deleted.
  • Page 85: Deleting Multiple Images All At One Time

     Image files on the SD card can be made read-only by accessing them from a PC. Read-only image files cannot be deleted by using the P-400R. If the images or folder selected for deletion include read-only files, a warning message appears when deletion is attempted.
  • Page 86: Deleting A Folder Containing Images

    P-400R Digital Microscope Instructions Press the [OK] button. A message confirming deletion appears. LCD panel [OK] button If you want to delete the images, select [Yes], and then press the [OK] button. If you want to cancel deletion, select [No], and then press the [OK] button.
  • Page 87 P-400R Digital Microscope Instructions Use the top and bottom buttons of the multi-selector to select the folder you want to delete. You cannot select more than one folder. Press the [OK] button. A message confirming deletion appears. LCD panel [OK] button If you want to delete the folder, select [Yes], and then press the [OK] button.
  • Page 88: Specifying The Playback Folder

    The [Playback menu] screen appears again. About settings of the playback folder The selection of the playback folder is reset. When the P-400R is turned on the next time, the active folder set as the default is used as the playback folder.
  • Page 89: Changing Settings

    P-400R Digital Microscope Instructions Changing Settings Using the setup menu The setup menu is used to specify the overall P-400R settings. Displaying the setup menu Press the [MENU] button to display the [Shooting menu] or [Playback menu] screen. LCD panel...
  • Page 90 P-400R Digital Microscope Instructions Restarting shooting/playback mode From the setup menu, to place the P-400R in playback mode again, press the capture switch. To place it in playback mode again, press the playback button ( Pressing the [MENU] button places the P-400R in the previous mode again.
  • Page 91: Adjusting The Brightness Of The Lcd Monitor

    P-400R Digital Microscope Instructions Adjusting the Brightness of the LCD Monitor You can adjust the brightness of the LCD monitor. The procedure is as follows. Display the [Setup menu] screen. Select [Monitor brightness], and then press the [OK] (or right) button.
  • Page 92: Selecting The Video Output Signal Format

    The [Setup menu] screen appears again. Selecting the Shutter Release Method You can select whether the capture switch on the P-400R or the optional release switch (P-RELS) is used to release the shutter. The procedure is as follows. Display the [Setup menu] screen.
  • Page 93: Initializing An Sd Card

    Setting the Automatic Power Save Function The P-400R has the automatic power save function, which automatically places the P-400R in standby mode if no operation is performed for a length of time. The following shows to procedure for enabling this function and specifying the time.
  • Page 94: Selecting The Language

    P-400R Digital Microscope Instructions Selecting the Language You can select the language of the text displayed in the LCD monitor. The procedure is as follows. Display the [Setup menu] screen. Select [Language], and then press the [OK] (or right) button.
  • Page 95: Checking The Version Information

    P-400R Digital Microscope Instructions Checking the Version Information You can check the version information of the P-400R firmware. The procedure is as follows. Display the [Setup menu] screen. Select [Firmware version], and then press the [OK] (or right) button. The version number of the P-400R firmware is displayed.
  • Page 96: Restoring The Initial Settings

    P-400R Digital Microscope Instructions 7.10 Restoring the Initial Settings You can restore the P-400R initial settings. The procedure is as follows. Display the [Setup menu] screen. Select [Reset all], and then press the [OK] (or right) button. A confirmation message appears.
  • Page 97: Connecting To A Pc

    P-400R Digital Microscope Instructions Connecting to a PC If the optional USB cable (P-CUS) is used to connect the P-400R to a PC, the images saved in the P-400R can be transferred to the PC. The procedure is as follows.
  • Page 98: About Shuttlepix Editor

    P-400R Digital Microscope Instructions About ShuttlePix Editor ShuttlePix Editor is a free application program provided by Nikon. ShuttlePix Editor operating environment ShuttlePix Editor can operate in the following environments. For details on how to install ShuttlePix Editor, see the ShuttlePix Editor documentation.
  • Page 99: Troubleshooting

    If a problem that is not listed in the table occurs or if a problem that occurred is not corrected by taking action as described in the table, remove the battery or disconnect the power cord of the product, and then contact your nearest Nikon representative. About the Power Supply and Battery...
  • Page 100: About The Image Display

    An external monitor is Disconnect the P-CV video cable from the connected. P-400R. Operate the power switch of the motorized focusing stand. See the The P-400R is When the P-400R is mounted on the...
  • Page 101: About Operating This Product

    Review the capture operation setting. setting is specified If capture operation is set to External, the incorrectly. capture switch of the P-400R is disabled. Similarly, if capture operation is set to Internal, the optional P-RELS Release cannot be used. The P-400R is...
  • Page 102 P-400R Digital Microscope Instructions Symptom Possible cause Action Ref. An image The shutter speed is If you use a slow shutter speed when blurs. too slow. capturing an object that is moving, the object blurs. Switch the exposure mode to Shutter-priority AE or Manual, and set a faster shutter speed.
  • Page 103: Image Saving

    P-400R Digital Microscope Instructions Image Saving Symptom Possible cause Action Ref. Data cannot be The SD card has not Insert the SD card correctly. written to the been inserted correctly. SD card or read The SD card has not Initializing the SD card.
  • Page 104: Warning Messages

    P-400R Digital Microscope Instructions Warning Messages This section explains about the meaning of the alert messages displayed on the LCD monitor and the actions to take to solve the problem. SD card related Message Meaning Action Ref. No SD card The SD card has not Insert the SD card correctly.
  • Page 105 P-400R Digital Microscope Instructions Image observation/capturing Message Meaning Action Ref. P OVER Overexposure is Since the object is too bright, an detected in Program appropriate exposure cannot be obtained in Program AE. Adjust the illumination. P UNDER Underexposure is Since the object is too dark, an...
  • Page 106 P-400R Digital Microscope Instructions Image playback/deletion Message Meaning Action Ref. No image There is no shot image The image was not in the playback – in the specified folder in or shooting folder at the time of the SD card.
  • Page 107 Use it under low and the battery the proper environment. temperature has become -10C or less. Transfer error A cable has been Turn off the power of the P-400R removed during before plugging or unplugging the communication with a cable.
  • Page 108: Daily Maintenance

    P-400R Digital Microscope Instructions Daily Maintenance To prolong the performance of this product, it is recommended to perform daily maintenance as described below. Cleaning utensils and consumables  Cleaning utensils Soft-tip brush (or a clean-room wiper in the clean room environment) ...
  • Page 109: Cleaning Painted, Printed, Or Plastic Parts

     During storage, place a plastic cover over the equipment to prevent dust accumulation. 10.5 Periodic Inspections (Charged) Periodic inspections are recommended in order to maintain the performance of this product. Consult your Nikon representative for details about periodic inspections.
  • Page 110: Major Specifications

    P-400R Digital Microscope Instructions Major Specifications For the specifications of the following products, see the manual of the individual product:  P-Li01 P-Li-ion Rechargeable Battery  P-CH01 P-BACH Battery Charger 11.1 P-400R Digital Microscope System Model name P-400R Digital Microscope...
  • Page 111 P-400R Digital Microscope Instructions Image quality control Scene mode 5 modes (Standard, Wafer/IC Chip, Metal Ceramic, Circuit Board, FPD) Custom setting 4 settings Aperture Resolution-priority mode / DOF-priority mode Exposure mode Program AE/Shutter-priority AE/Manual Metering method Average photometry/peak-hold photometry with AE lock functionality...
  • Page 112: Power Supply

    P-400R Digital Microscope Instructions Illumination optics Light source Eight white LEDs Illumination method Ring illumination method Illumination area Diameter: 20 mm, four-way (top/bottom/left/right) polarized illumination possible Illumination intensity Off, 10 levels (1 to 10, 10 is a maximum.) control LCD monitor LCD panel 2.66-in.
  • Page 113 P-400R Digital Microscope Instructions Applied Standards This product complies with FCC Part 15B Class A. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
  • Page 114: Ac Adapter

    P-400R Digital Microscope Instructions 11.2 AC Adapter Model name EA1050E-120 Manufacturer EDAC POWER Electronics Co., Ltd. Rated input voltage 100-240 VAC, 50/60 Hz, 1.8 A, max. Rated output voltage 12 VDC, 3.5 A Max. Power cord For regions where the power supply is 100 to 120 VAC: Use a UL-listed detachable power cord set.
  • Page 115: Supported Standards

    P-400R Digital Microscope Instructions 11.4 Supported Standards  DCF (Design rule for Camera File system): This recording system allows image files recorded by digital cameras from different manufacturers to be used mutually.  Exif (Exchangeable image file format) Version 2.2: The goal of this standard is to enhance the linkage between digital cameras and printers to easily obtain high-quality print output.

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