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2
Focusing on the Specimen (Vertical Stage Movement)
Notes on controlling the focus knobs
Avoid the following actions, which can cause equipment
malfunctions.
Rotating the right and left focus knobs in opposite directions.
Rotating the coarse focus knob past the limit.
Turn the coarse or fine focus knob to raise or lower the stage and
shift the focus onto the specimen.
Using an objective of high magnification may cause the specimen
to be pushed against the objective, damaging the objective.
Follow the procedure below to focus on the specimen to avoid
breaking the cover glass or damaging the objective.
(1)
Bring the 10x objective into the optical path and turn the
coarse focus knob away to raise the stage to the upper limit.
(2)
When using the trinocular tube or ergonomic tube, push in
the optical path switching lever to distribute 100% light to the
binocular section.
(3)
Look into the eyepiece, and adjust the brightness of the field
of view by turning the dia-illumination brightness control
knob.
(4)
Look into the eyepiece and turn the coarse focus knob slowly
to lower the stage and focus on the specimen. Release your
hand from the coarse focus knob once it has been focused.
Chap. 2
(5)
Turn the fine focus knob to adjust the focus more accurately.
Raising the stage with the coarse focus knob
When moving the stage with the coarse focus knob, move your eyes away from the eyepiece and operate the
microscope while looking at it from the side.
Performing coarse operation while looking into the eyepiece
When working with the coarse focus knob while looking into the eyepiece, you should only turn the knob in the direction
for lowering the stage.
Switching to an objective of higher magnification
First use an objective of low magnification to adjust the focus, then switch to an objective of higher magnification.
Operating distance
As a 10x or 4x objective has a wider operating distance, the specimen never touches the tip of the objective even if the
stage is raised to the upper limit, provided that a slide and a cover glass of standard thickness are used. (Standard
thickness being 1.2 mm for the slide glass, and 0.17 mm for the cover glass.)
Chapter 2
Individual Operations
40
0.9
0.8
0.7
0.6
0.4
0.3
POWER
Don't rotate the knobs in opposite directions!
Darker
(1)
Select the 10x objective
and use the coarse
focus knob to raise the
stage to the upper limit.
Bringing the 10x objective into the optical path,
raising the stage to the upper limit
0.9
0.8
POWE R
Using the coarse focus knob to focus→Using the
fine focus knob for more accurate focus
0.1
0.2
10x
0.1
0.2
0.6
0.5
0.7
0.9
0.8
POWE R
Brighter
(3)
Adjust the brightness
of the field of view.
0.1
0.2
0.5
0.7
0.6
(4)
Focusing with the
coarse focus knob
(5)
Focusing with the
fine focus knob for
more accurate
focus
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