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7
Selecting a Condenser
Select a condenser optimal for the magnification of the objective and the microscopy procedure.
Objective's
Achromat
magnification
condenser
1x
2x
4x
10x to 100x
*1:
The entire field of view may not be covered if a UW eyepiece is attached.
*2:
Swing out the top lens before use.
*3:
For objectives of 10x or higher magnification, pull out the slide. For objectives with 2x or 4x magnification,
push in the slide to prevent vignetting.
*4:
Do not use the 2-100x slide achromat condenser with an achromat objective or a plan achromat objective.
Depending on the type of objective, the indicated numerical aperture of the objective may not be achieved.
For example, when an objective with a numerical aperture of 1.4 is used, the maximum aperture of the swing-out
condenser or the Abbe condenser will only be about 65% of the NA of the objective, even when the condenser's aperture
diaphragm is wide open.
Refer to Section 16 "Tips for the Phase Contrast Microscopy" for the phase turret condenser.
8
Adjusting the Field Diaphragm
Chap. 2
The field diaphragm is used to restrict illumination to the area of
the specimen being viewed.
Turning the field diaphragm dial changes the size of the field
diaphragm.
For normal observations, the size of the diaphragm should almost
circumscribe the field of view.
Proper size of the field diaphragm
Usually, the size is optimal when it almost circumscribes the field of view. Opening the field diaphragm too much and
illuminating a broader area than necessary will result in stray light entering the field of view, generating flare and
reducing the image contrast. In addition, the specimen will become decolorized over a wider area.
Adjustment timing for the field diaphragm
Be sure to adjust the aperture diaphragm each time you change the objective.
Chapter 2
Selecting the Magnification of the Objective and Condenser
Condenser (: Optimum, ○: Suitable, ×: Not suitable)
Swing-out
aplanatic
condenser
×
×
×
○*2
×
○
Individual Operations
Motorized
Achromat
swing-out
condenser
condenser
×
×
×
○*2
○*1
○
○
Field diaphragm dial
48
Swing-out
Abbe
condenser
condenser
1-100x
×
○*2
×
*2
○*1
○
0.9
0.8
0.5
0.4
0.3
0.2
0.1
Adjusting the field diaphragm
Slide achro
condenser
2-100x
*4
×
*3
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