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Zeiss Axiotech Operating Manual

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Axiotech and Axiotech
Materials Microscopes
Operating Manual

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Summary of Contents for Zeiss Axiotech

  • Page 1 Axiotech and Axiotech Materials Microscopes Operating Manual...
  • Page 2 Carl Zeiss Axiotech and Axiotech Knowledge of this manual is required for the operation of the instrument. Please therefore make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 3: Table Of Contents

    Title page ..........................I Copyright ........................... II Table of contents........................III Notes on instrument safety ....................VII Notes on warranty ......................X Overall view of Axiotech 30 ....................XI Overall view of Axiotech 100 .................... XII vario Overall view of Axiotech 100..................XIII INSTRUMENT DESCRIPTION ..................
  • Page 4 Transmitted-light illumination equipment ............... 2-27 2.13.1 Change of 6 V 30 W halogen lamp in the stand base (Axiotech 30 and Axiotech 100) ..2-27 2.13.2 Connecting the 6 V 30 W halogen illuminator to the line (Axiotech 30 and Axiotech 100)2-28 vario 2.13.3...
  • Page 5 Carl Zeiss Page OPERATION ........................3-3 Overview of operation and function controls ..............3-3 3.1.1 Operation and function controls of the Axiotech 30 ............3-3 3.1.2 Operation and function controls of the Axiotech 100 ............3-4 vario 3.1.3 Operation and function controls of the Axiotech 100............
  • Page 6 Carl Zeiss Axiotech and Axiotech Page CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-3 Instrument care ....................... 4-3 Instrument maintenance....................4-4 4.2.1 Performing checks......................4-4 4.2.2 Changing the fuses ......................4-4 4.2.3 Check instrument voltage and change it, if required............4-5 Troubleshooting ......................

  • Page 7: Notes On Instrument Safety

    Carl Zeiss Notes on instrument safety vario The Axiotech and Axiotech microscopes were designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meet the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives.
  • Page 8 Carl Zeiss microscopy service for the repair of the instrument. The wide-range power unit which is integrated in the stand of the Axiotech 30 microscope permits the use of line voltages in the range between 100 and 240 V ± 10%, 50 - 60 Hz, without the need for the voltage to be changed at the instrument.
  • Page 9 The instruments must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application. The Axiotech and vario...

  • Page 10: Notes On Warranty

    This forfeits all the claims against warranty. With the exception of the work specified in this manual, no maintenance or repair of the Axiotech or vario Axiotech may be performed.
  • Page 11 Axiotech and Axiotech Carl Zeiss Overall view of the Axiotech 30 reflected-light microscope B 40-020 e 06/99...
  • Page 12 Carl Zeiss Axiotech and Axiotech Overall view of the Axiotech 100 reflected-light microscope B 40-020 e 06/99...

  • Page 13: Overall View Of Axiotech 30

    Axiotech and Axiotech Carl Zeiss vario Overall view of the Axiotech 100 reflected-light microscope B 40-020 e 06/99 XIII...
  • Page 14 Carl Zeiss Axiotech and Axiotech B 40-020 e 06/99...
  • Page 15 Axiotech and Axiotech Carl Zeiss INSTRUMENT DESCRIPTION Contents INSTRUMENT DESCRIPTION ..................1-3 Name and intended application..................1-3 Instrument description and main features.................1-4 Microscope components and modules ................1-5 Objectives........................1-11 Eyepieces........................1-13 Condensers ........................1-13 Stage micrometers and eyepiece reticles................. 1-14 Microscope stages and specimen holders ............... 1-16 Binocular tubes......................
  • Page 16 Carl Zeiss Axiotech and Axiotech B 40-020 e 06/99...

  • Page 17: Vario 100

    The Axiotech and Axiotech instruments fit as follows into the line of upright microscopes from Carl Zeiss: Axiolab A vario Axiotech 30, Axiotech 100 and Axiotech Axioplan 2 Axiophot 2 vario The Axiotech and Axiotech technical microscopes in a modular design feature a sturdy stand and a vario medium-size stand.

  • Page 18: Instrument Description And Main Features

    ICS line can also be used. Depending on the configuration, Axiotech microscopes are equipped with - or prepared for - a 30 W or 100 W halogen illuminator for reflected-light applications and with a 30 W halogen illuminator for transmitted-light applications.

  • Page 19: Microscope Components And Modules

    Axiotech and Axiotech Carl Zeiss Microscope components and modules Fig. 1-1 Microscope components and modules (sheet 1) B 40-020 e 06/99...

  • Page 20: Fig. 1-2 Microscope Components And Modules (Sheet 2)

    Carl Zeiss Axiotech and Axiotech Fig. 1-2 Microscope components and modules (sheet 2) B 40-020 e 06/99...

  • Page 21: Fig. 1-3 Microscope Components And Modules (Sheet 3)

    Axiotech and Axiotech Carl Zeiss D IC D IC Fig. 1-3 Microscope components and modules (sheet 3) B 40-020 e 06/99...

  • Page 22: Fig. 1-4 Microscope Components And Modules (Sheet 4)

    Carl Zeiss Axiotech and Axiotech H IP S 23 i s o l a t Fig. 1-4 Microscope components and modules (sheet 4) B 40-020 e 06/99...

  • Page 23: Fig. 1-5 Microscope Components And Modules (Sheet 5)

    Axiotech and Axiotech Carl Zeiss D IC S ta te rn Fig. 1-5 Microscope components and modules (sheet 5) B 40-020 e 06/99...
  • Page 24 Configurations 491003-9804-000 Axiotech 30 microscope for brightfield and darkfield in reflected light 000000-1061-197 Axiotech 30 microscope for brightfield and darkfield in reflected light and brightfield in transmitted light 491004-9804-000 Axiotech 30 microscope for brightfield, darkfield and DIC in reflected light...

  • Page 25: Objectives

    Epiplan 10×/0.20 HD ∞/- where 10× is the objective magnification, with a defined color ring on the objective being allocated to each magnification step (color coding system from Carl Zeiss) 0.20 is the numerical aperture stands for reflected light, brightfield and darkfield objective ∞...

  • Page 26: Objectives

    Carl Zeiss Axiotech and Axiotech vario The following objectives are available for Axiotech and Axiotech Objective Magnification/ Cover slip Free working Contrasting Cat. No. Num. aperture thickness D distance technique in mm in mm Epiplan Epiplan 19.8 HF, DIC 442920-0000-000 5×/0.13...

  • Page 27: Eyepieces

    444142-0000-000 Micrometer eyepiece 20× If required, eyecups for the eyepieces can be ordered under Cat. No. 444801-0000-000. Condensers The following condensers are available for Axiotech for combination with stage carrier D and the transmitted-light equipment: Condenser Cat. No. Application range Notes ABBE condenser 0.9/1.25 with...

  • Page 28: Stage Micrometers And Eyepiece Reticles

    Carl Zeiss Axiotech and Axiotech Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small selection of which is listed below: Illustration Description, Technical Data Cat. No. Stage micrometer for reflected light,...
  • Page 29 Axiotech and Axiotech Carl Zeiss Net micrometer 12.5 × 12. 5 / 5; 10 / 474068-0000-000 d = 26 mm area 12.5 × 12,5 mm, divided in fields of 5 × 5 or 10 × 10 454075-0000-000 Photo reticle MC 2.5× / d = 26 mm for 35 mm photography with an additional magnification of 2.5×...

  • Page 30: Microscope Stages And Specimen Holders

    Carl Zeiss Axiotech and Axiotech Microscope stages and specimen holders The microscopes in the Axiotech line can be equipped with the following microscope stages and specimen holders: Description Cat. No. Notes Reflected-light mechanical stage 453527-0000-000 contained in the delivery package of the 75 ×...

  • Page 31: Binocular Tubes

    Binocular tubes The binocular phototube 20°/23 with image erection and sliding prism is a standard feature of the microscopes in the Axiotech line. The following binocular tubes and intermediate tubes can also be used vario with the Axiotech and Axiotech Description Cat.

  • Page 32: Reflector Sliders And Microscopy Techniques

    Carl Zeiss Axiotech and Axiotech 1.10 Reflector sliders and microscopy techniques Description Cat. No. Major microscopy techniques Reflector slider HD 446343-0000-000 reflected-light brightfield, reflected-light darkfield, transmitted-light brightfield, transmitted-light darkfield, transmitted-light phase contrast Reflector slider HD DIC 446342-0000-000 reflected-light brightfield, reflected-light darkfield,...

  • Page 33: Technical Data

    Axiotech and Axiotech Carl Zeiss 1.11 Technical Data Dimensions (width × depth × height) Axiotech stand................... approx. 210 × 315 × 435 mm stand ..................approx. 412 × 452 × 375 mm vario Axiotech Axiotech footprint....................approx. 250 × 350 mm footprint ....................
  • Page 34 Line frequency........................50 to 60 Hz Power consumption using internal power supplies: Axiotech 30 (6 V 30 W)......................80 VA Axiotech 100 (12 V 100 W) ..................... 225 VA and external power supplies: power supply 6 V 10...40 W, 2 ports, switchable ............... 50 VA power unit for HBO 50, 220...240 V..................
  • Page 35 Axiotech and Axiotech Carl Zeiss HBO 50 mercury pressure short-arc lamp lamp voltage for lamp types L1 and L2............L1: 39...45 V/L2: 34...39 V lamp current for lamp types L1 and L2............... L1: 1.30 A/L2: 1.45 A output ............................50 W average light current ......................
  • Page 36 Carl Zeiss Axiotech and Axiotech Binocular tubes (selection): Binocular tube 30°/23; maximum field number ......................23 interpupillary distance............... can be set between 55 and 75 mm Viewing angle .......................... 30° Viewing port ......................tube factor 1× Binocular phototube 20°/23 with image erection and sliding prism (100:0/0:100);...

  • Page 37: Start-Up

    Attachment, change and centering of the microscope stage........... 2-12 2.8.1 Axiotech 30 and Axiotech 100 with stage carrier A............2-12 2.8.2 Axiotech 30 and Axiotech 100 with stage carrier D and adapter ........2-13 vario 2.8.3 Axiotech 100 with stage carrier H = 140 mm.............. 2-14 2.8.4...
  • Page 38 Carl Zeiss Axiotech and Axiotech 2.13.1 Change of 6 V 30 W halogen lamp in the stand base (Axiotech 30 and Axiotech 100) ..2-27 2.13.2 Connecting the 6 V 30 W halogen illuminator to the line (Axiotech 30 and Axiotech 100)..................2-28 vario 2.13.3...

  • Page 39: Unpacking The Microscope

    Unpacking the microscope 2.1.1 Axiotech 30 and Axiotech 100 • Open the transport case (2-1/1) and take out the accessories lying on top. • Take out the cardboard inserts (2-1/3).

  • Page 40: Setting Up The Microscope

    • The dust covers and sliders contained in the reflected-light equipment (2-3/1) remain in the instrument. Fig. 2-3 Setting up the microscope • Remove the rubber foam padding from below the stage of the Axiotech after moving the stage upwards. vario 2.2.1 Axiotech - Attaching the microscope body to column X95 •...

  • Page 41: Screw In Objectives

    Axiotech and Axiotech Carl Zeiss Screw in objectives • Remove dust covers (2-5/4) from the objectives inserted in the 5-position nosepiece. • Screw HD objectives (2-5/5) with M 27 × 0.75 thread in the nosepiece (2-5/3) starting with the lowest magnification factor.

  • Page 42: Attachment Of Intermediate Tube For Image Projection

    000) to remove the tube lens (2-7/2) from the binocular tube and store the tube lens in the case. • Insert dovetail (underside) of the adapter plate (2-7/10) into the stand port of the Axiotech and slightly tighten clamping screw (2-7/12). • Insert fixation...

  • Page 43: Attachment Of Optovar Intermediate Tube

    (2-8/2) from the binocular phototube (2-8/1) and store the tube lens in the case. • Insert dovetail (underside) of the adapter plate (2-8/9) into the stand port of the Axiotech and slightly tighten clamping screw (2-8/11). • Insert fixation...

  • Page 44: Insertion Of Eyepieces

    Carl Zeiss Axiotech and Axiotech Insertion of eyepieces All Axiotech microscopes are equipped with focusing eyepieces. An overview of available eyepieces is given in section 1.5. • Remove both protection caps (2-9/1) from the binocular phototube (2-9/2). • Insert focusing eyepieces (2-9/4) into the eyepiece tubes (2-9/3).

  • Page 45: Insertion Of The Eyepiece Reticle

    Axiotech and Axiotech Carl Zeiss 2.5.1 Insertion of the eyepiece reticle The eyepieces W-PL 10×/23 Br. foc. and E-PL 10×/20 Br. foc. are intended for use with eyepiece reticles (overview: section 1.7). The slight image shift caused by the additional...

  • Page 46: Compensation Of Ametropia When Eyepiece Reticles Are Used

    Carl Zeiss Axiotech and Axiotech 2.5.2 Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces, e.g. W-PL 10×/23 Br. foc., to enable compensation of ametropia. • Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used.

  • Page 47: Attachment Of Condenser

    Axiotech and Axiotech Carl Zeiss Attachment of condenser Fig. 2-13 Attachment of condenser • Remove condenser, e.g. swing-in version 0.9 Z Pol, from the packaging. • Carefully move microscope stage and stage carrier D to the uppermost stop position via the coarse drive (2-13/1).

  • Page 48: Attachment, Change And Centering Of The Microscope Stage

    • Fix condenser in position with the clamping screw (2-13/4) and move it close to the uppermost stop position via the condenser drive (2-13/2). Section 1.6 contains an overview of condensers available for the Axiotech. The maximum specimen height for use of stage carrier D is 39 mm.

  • Page 49: Axiotech 30 And Axiotech 100 With Stage Carrier D And Adapter

    Carl Zeiss 2.8.2 Axiotech 30 and Axiotech 100 with stage carrier D and adapter • Screw adapter for stage carrier D (2-15/1) to the Axiotech microscope stand with the semi- round recess showing upwards. For this purpose, tighten both hexagonal...

  • Page 50: Axiotech

    (modular dimensions) which allow not only the use of standard microscope stages (as included in the vario configurations) with the Axiotech but also stages from Märzhäuser and Mitutoyo. • Screw microscope stage (2-17/1) on the upper part of the stage carrier (2-17/2) through the...

  • Page 51: Centering Of Pol Rotary Stage

    Centering of Pol rotary stage vario The Axiotech and Axiotech microscopes permit use of the Pol rotary stage. This stage has been precentered in the factory, i.e. a centered object detail will remain – within certain tolerances - in the center of the field of view after stage rotation.

  • Page 52: Change Of Filters

    Carl Zeiss Axiotech and Axiotech Change of filters 2.9.1 Equip the filter slider with filters and push it in the beam path • Equip filter slider (2-20/3) with the filters (2-20/2) required for the relevant microscopy technique, e.g. with −...
  • Page 53 Axiotech and Axiotech Carl Zeiss The reflector sliders HD (2-21/1) and HD DIC (2-21/2) can only be pulled out if one of the dust covers (2-21/5) on the left or right stand side has been removed. For this purpose, unscrew the...

  • Page 54: Setting Of Reflected-Light Luminous-Field Diaphragm

    The luminous-field diaphragm can be centered using two centering screws (2-22/1). Fig. 2-22 Setting of reflected-light luminous- field diaphragm of the Axiotech 30 vario 2.10.2 Axiotech 100 and Axiotech • The luminous-field diaphragm is adjusted by turning the knurled wheel (2-23/2) which is marked with increments for rough orientation: −...

  • Page 55: Connection To The Line And Switching The Instruments On And Off

    Connection to the line and switching the instruments on and off 2.11.1 Connection of Axiotech 30 The Axiotech microscope features an integrated wide-range power unit, i.e. it can be used with line voltages ranging from 100 to 240 V 50 ... 60 Hz. The wide-range power unit sets itself automatically to the relevant line voltage.

  • Page 56: Connection Of Axiotech 100

    2.11.2 Connection of Axiotech 100 The Axiotech 100 microscope features an integrated stabilized 12 V 100 W power unit which uses the two switchable voltage ranges 100 ... 127 V AC or 220 ... 240 V AC. Before the instrument is switched on for the first time, it is therefore absolutely necessary to...

  • Page 57: Vario 100

    Axiotech and Axiotech Carl Zeiss vario 2.11.3 Connection of Axiotech vario The Axiotech 100 microscope has no integrated power unit , i.e. all the usable illuminators are operated via external power units. Before the instrument is switched on for the first time, it is therefore absolutely necessary to...

  • Page 58: Reflected-Light Illumination Equipment

    Carl Zeiss Axiotech and Axiotech 2.12 Reflected-light illumination equipment 2.12.1 Change of 6 V 30 W halogen lamp (Axiotech 30) • Switch off the On/Off switch (2-28/8) on the microscope stand disconnect instrument cable (2-28/7) from the line. • Allow hot halogen illuminator to cool down for approx.

  • Page 59: Attachment Of 12 V 100) W Halogen Illuminator, Adjustment And Change Of 12 V 100) W Halogen Lamp (Axiotech 100) And Axiotech 100))

    Attachment of 12 V 100 W halogen illuminator, adjustment change of 12 V 100 W halogen vario lamp (Axiotech 100 and Axiotech 100) Attachment of 12 V 100 W halogen illuminator • Loosen hexagonal screw (2-29/2) using ball- headed screwdriver (2-29/1) and remove dust protection cap (2-29/3).
  • Page 60 • Use 10x objective to focus on the specimen, search an empty object spot and check the illumination of the field of view. Detailed information on the operation and alignment is contained in operating manual G 42- 216 from Carl Zeiss, entitled " HAL Microscope Illuminator with Reflector". 2-24 B 40-020 e 06/99...
  • Page 61 Axiotech and Axiotech Carl Zeiss Change of 12 V 100 W halogen lamp • Switch off the lamp supply via the On/Off switch of the microscope (see section 2.11). • Disconnect the line cable from the line. • Allow the hot 12 V 100 W halogen illuminator to cool down for approx.

  • Page 62: Attachment And Connection Of N Xbo 75 Xenon Short-Arc Illuminator

    The N XBO 75 Xenon short-arc illuminator contains the XBO 75 gas discharge lamp with a high radiation intensity and daylight characteristics (5500 K). Electrical power is supplied by the N XBO 75 transformer. • The N XBO 75 Xenon short-arc illuminator can be connected to the Axiotech or Axiotech vario microscope stand via the mechanical dovetail mount.

  • Page 63: Transmitted-Light Illumination Equipment

    Carl Zeiss 2.13 Transmitted-light illumination equipment 2.13.1 Change of 6 V 30 W halogen lamp in the stand base (Axiotech 30 and Axiotech 100) In the Axiotech 30 instrument configuration for brightfield and darkfield in reflected light, the microscope stand...
  • Page 64 Carl Zeiss Axiotech and Axiotech 2.13.2 Connecting the 6 V, 30 W halogen illuminator to the line (Axiotech 30 and Axiotech 100) Power supply halogen illuminator in transmitted light is always performed via the separate power unit and not via the integrated...

  • Page 65: Change Of 6 V 30 W Halogen Lamp (Axiotech 100)

    Change of 6 V 30 W halogen lamp vario (Axiotech 100) vario The transmitted-light equipment of the Axiotech consists of the following components: • Transmitted-light equipment (2-34/1) • 6 V 30 W halogen lamp and • 6 V 10 ... 40 W power supply (2-34/2), either for 230 V or 120 V line voltage.

  • Page 66: Connection Of Power Supply For 6 V 30 W Halogen Illuminator (Axiotech 100)

    Axiotech and Axiotech vario 2.13.4 Connection of power supply for 6 V 30 W halogen illuminator (Axiotech 100) Before connecting and switching on the 6 V 10 ... 40 W power supply, check whether the primary power supply instrument voltage complies with the line voltage! If this is not the case, contact your nearest Carl Zeiss agency immediately.

  • Page 67: Operation

    Operation and function controls of the Axiotech 100............ 3-5 Switch on the microscope ....................3-6 3.2.1 Switch on the Axiotech 30 and Axiotech 100 ..............3-6 vario 3.2.2 Switch on the Axiotech 100 (separate SNT 12 V DC, 100 W power unit, stabilized) ..3-6 Setting the microscope (basic settings) ........
  • Page 68 Fig. 3-3 Operation and function controls of the Axiotech 100 ........... 3-5 Fig. 3-4 Switch on the Axiotech 30 and Axiotech 100 ..............3-6 vario Fig. 3-5 Switch on the Axiotech 100 HD ................... 3-6 Fig.

  • Page 69: Overview Of Operation And Function Controls

    Axiotech and Axiotech Carl Zeiss OPERATION Overview of operation and function controls 3.1.1 Operation and function controls of the Axiotech 30 1 Eyepieces On/Off switch with pilot lamp 2 Binocular phototube 20° Coaxial coarse and fine drive 3 Pushrod (to change beam path)

  • Page 70: Operation And Function Controls Of The Axiotech 30

    Carl Zeiss Axiotech and Axiotech 3.1.2 Operation and function controls of the Axiotech 100 1 Eyepieces 11 Reflected-light illuminance control 2 Binocular phototube 20° 12 On/Off switch with pilot lamp 3 Pushrod (to change the beam path) 13 Coaxial coarse and fine drive...

  • Page 71: Operation And Function Controls Of The Axiotech 30

    Axiotech and Axiotech Carl Zeiss vario 3.1.3 Operation and function controls of the Axiotech 1 Eyepieces 9 Pushrod for reflected-light aperture diaphragm 2 Binocular phototube 20° 10 Luminous-field diaphragm slider with 3 Pushrod (to change the beam path)) centering screws and knurled wheel for reflected light...

  • Page 72: Vario 100

    Switch on the microscope 3.2.1 Switch on the Axiotech 30 and Axiotech 100 • Switch on the Axiotech 30/100 via the On/Off switch (3-4/4): − the green pilot lamp in the switch must light up and − depending on the position of the illuminance control (3-4/3), at least one LED of the lamp voltage display (3-4/2) must light up.

  • Page 73: Setting The Microscope (Basic Settings)

    Setting the microscope (basic settings) The description of the steps required for the various illumination and contrasting techniques is based on the following microscope settings: • As described in chapter 2, the Axiotech or Axiotech vario microscope is ready for operation and switched on as described in section 3.2.
  • Page 74 Carl Zeiss Axiotech and Axiotech In reflected-light brightfield, the KÖHLER illumination principle requires the following settings of the luminous-field diaphragm and the aperture diaphragm: • Perform steps described in sections 3.2 and 3.3. • Move reflector slider HD DIC (3-7/5) or HD (3-7/6) in the brightfield position ( H mark visible on the reflector slider).
  • Page 75 Axiotech and Axiotech Carl Zeiss • Focus on the specimen via the coaxial drive (3-7/4). If possible, always move the specimen away from the objective to avoid a collision between the objective and the specimen. • Move aperture diaphragm (3-7/2) in center position (approx. half opened or half closed) by pulling out the pushrod.

  • Page 76: Setting Of Reflected-Light Darkfield

    Carl Zeiss Axiotech and Axiotech 3.4.2 Setting of reflected-light darkfield The reflected-light darkfield technique is ideal for the inspection of surfaces. The illumination light coming from the reflected-light illuminator is directed into the darkfield channel of the HD objective ( 3-9/4) via a component combined of a prism system (3-9/2) and a ring mirror ( 3-9/3).
  • Page 77 Axiotech and Axiotech Carl Zeiss Fig. 3-10 Setting of reflected-light darkfield • Remove any light and/or attenuation filter which might be in the beam path, i.e. pull out the filter slider (3-10/3) completely or switch to the free light path position.

  • Page 78: Setting Of Reflected-Light Interference Contrast

    An additionally inserted lambda plate ( 3-11/7a) changes the gray values into colors again. vario The Axiotech and Axiotech allow differential interference contrast to be performed with all objectives with magnification ≥ 5×. • Perform steps described in sections 3.2 and 3.3.
  • Page 79 Axiotech and Axiotech Carl Zeiss Version 1: • Remove any light/attenuation filters from the beam path, i.e. pull out the filter slider or switch to the free light path position. • Push HD DIC reflector slider into the reflected-light illuminator until the DIC position is active ( DIC mark visible on the reflector slider).
  • Page 80 Carl Zeiss Axiotech and Axiotech • Swing required objective in the beam path . • Focus on the specimen via the coaxial coarse/fine drive. • Turn large knurled knob of DIC prism slider until the field center appears as dark as possible.

  • Page 81: Setting Of Reflected-Light Polarization Contrast

    Axiotech and Axiotech Carl Zeiss 3.4.4 Setting of reflected-light polariza- tion contrast Reflected-light polarization contrast is the ideal technique for surface structures which change the state polarization during reflection. The illumination light ( 3-13/1) is linearly polarized by the polarizer ( 3-13/2) and, after passing the objective (3-13/4), hits the sample surface ( 3-13/5) where it is reflected.
  • Page 82 Carl Zeiss Axiotech and Axiotech Polarizer and analyzer are firmly integrated in the reflector slider with the appropriate orientation to each other. • Place specimen on the stage, set the required magnification, focus and view the specimen in the polarization contrast now present.

  • Page 83: Setting Of Epi-Fluorescence

    The epi-fluorescence technique is only described for the Axiotech 100 and the Axiotech 100, since only these models permit attachment of different illuminators for fluorescence. The following components are required for epi-fluorescence: •...
  • Page 84 Setting of epi-fluorescence using the FL filter slider • Perform steps described in sections 3.2 and 3.3. • If required, replace HAL 12 V 100 W halogen lamp ( 3-16/2) of the Axiotech 100 or Axiotech vario with the HBO 50 illuminator (also see operating manual G 42-160 entitled "Microscope Illuminator with HBO 50").
  • Page 85 Axiotech and Axiotech Carl Zeiss 3.4.5.2 Setting of epi-fluorescence using the 3 FL reflector slider • Perform steps described in sections 3.2 and 3.3. • The 3 FL reflector slider (3-17/3) replaces the reflector slider HD or HD DIC.
  • Page 86 Carl Zeiss Axiotech and Axiotech The 3 FL reflector slider has three stop positions, with the center position being equipped with the FT 395 chromatic beam splitter for reflected-light brightfield. This short-wave chromatic beam splitter permit "quasi" brightfield illumination. If required, this reflector slider position can be upgraded in a filter set for UV excitation by the insertion of filters.

  • Page 87: Change Of Filters In The Reflector Slider

    Axiotech and Axiotech Carl Zeiss 3.4.6 Change of filters in the reflector slider In its brightfield position, the HD ( 3-19/1) or HD DIC (3-19/2) reflector slider contains an integrated attenuation fi lter D = 0.25 (25 % transmission), dia.

  • Page 88: Setting Of Transmitted-Light Brightfield For Köhler Illumination

    Carl Zeiss Axiotech and Axiotech 3.4.7 Setting of transmitted-light brightfield for Köhler illumination Transmitted-light brightfield microscopy is the most usual of all the optical techniques, since it allows the easy and fast viewing of high-contrast or stained specimens. In transmitted-light brightfield, the KÖHLER illumination principle requires the following settings of the condenser, the luminous-field diaphragm and the aperture diaphragm: •...
  • Page 89 Axiotech and Axiotech Carl Zeiss • Remove the eyepiece, look into the tube and use the centering telescope to center the aperture diaphragm with the exit pupil opening using the two knurled screws ( 3-21/2). Then use the knurled ring (3-21/1) to set the aperture diaphragm to approx.

  • Page 90: Setting Of Transmitted-Light Polarization Contrast

    3.4.8.1 Transmitted-light polarization contrast with the reflector slider HD DIC on the Axiotech 30 and Axiotech 100 The rotary polarizer D ( 3-23/13) or the swing-in polarizer (3-23/6) are available to choose from for the Axiotech 30 and 100.
  • Page 91 Axiotech and Axiotech Carl Zeiss Fig. 3-23 Attach polarizer to stage carrier D • Attach selected polarizer to the underside of the stage carrier D with screws (3-23/5 and 8 or 10) and stop screw (3-23/11). • Place specimen to be examined on the microscope stage and fix it.
  • Page 92 ( 3-24/4) to be inserted in the polarization contrast with reflector reflector slider HD DIC until stop on the left vario slider HD DIC (Axiotech 100) side. The adjusting lever ( 3-24/5) of the ± 10°rotary analyzer slider permits the image contrast to be further optimized with crossed polars.
  • Page 93 3.4.8.3 Transmitted light polarization contrast using reflector slider without DIC position on the Axiotech 30 and Axiotech 100 Polarization contrast using the reflector slider without DIC position in combination with the transmitted-light equipment is possible with the ± 10° rotary analyzer slider, Cat. No. 453686- 0000-000.
  • Page 94 Carl Zeiss Axiotech and Axiotech 3.4.8.4 Transmitted-light polarization contrast using reflector slider without DIC position vario on the Axiotech Polarization contrast using the reflector slider without DIC position in combination with the transmitted-light equipment is possible with the ± 10° rotary analyzer slider, Cat. No. 453686- 0000-000.

  • Page 95: Setting Of Transmitted-Light Phase Contrast

    Configurations Depending on which condenser is used, the phase contrast technique requires the following modules and can therefore only be performed with the Axiotech 30 and the Axiotech 100. • For the ABBE condenser 0.9/1.25 with 5-position turret disk, Cat. No. 445303-0000-000:...

  • Page 96: Microscopy

    Make sure to avoid finger prints in particular. 3.4.10 3D Microscopy vario Detailed information on 3D microscopy using the Axiotech 100 and Axiotech 100 is contained in the following operating manuals (B) and brief instructions (K) from Carl Zeiss; Axiotech 100:...

  • Page 97: Attachment Of Photomicrography Equipment

    Carl Zeiss Attachment of photomicrography equipment vario The Axiotech and Axiotech microscopes can be switched from observation to photomicrography via a pushrod ( 3-29/7) on the phototube (photomicrography function: pushrod pulled out). Special adapters allow commercially available 35-mm SLR cameras and special microscope cameras (e.g. MC 80 ) to be attached to the camera port.
  • Page 98 T2 adapter for NIKON (F bayonet) 416009-0000-000 T2 adapter for PENTAX (KA bayonet) 416011-0000-000 For detailed information on SLR cameras, please see the new operating manual B 40-046 entitled "Photomicrography using 35 mm SLR Cameras" from Carl Zeiss. 3-32 B 40-020 e 06/99...

  • Page 99: Attachment Of Mc 80 Dx Microscope Camera (Large Format With 4" × 5" Large-Frame Attachment)

    (3-30/12). • Connect line cable of control panel (3-30/13) to the line. For detailed information on the MC 80 , please see operating manual B 40-036, MC 80 Microscope Camera, from Carl Zeiss. B 40-020 e 06/99 3-33...
  • Page 100 • Insert a film. • Connect cable (3-31/11) to the line. detailed information on the MC 80 please operating manual B 40-036, MC Microscope Camera, from Carl Zeiss. Fig. 3-31 Attachment of MC 80 microscope camera (large format) 3-34 B 40-020 e 06/99...

  • Page 101: Attachment Of Videomicroscopy Equipment

    Carl Zeiss Attachment of videomicroscopy equipment vario Videomicroscopy with the Axiotech and Axiotech microscopes can be performed using TV adapters and video zoom adapters. Video zoom adapters with continuously variable factors between 0.4 and 2.0× permit the standard magnification to be transferred to video prints.

  • Page 102: Selection And Assembly Of Adapters For Video Cameras

    Selection and assembly of adapters for video cameras The following video adapters and video zoom adapters with 60 mm interface permit the connection of 1-chip b/w and color CCD cameras and 3-chip color CCD cameras to the phototube of the Axiotech and vario...

  • Page 103: Attachment Of Video Camera

    Axiotech and Axiotech Carl Zeiss The connecting piece 60 - 44 also allows video adapters with 44 mm interface to be used with the vario phototube of the Axiotech and Axiotech with 60 mm interface. Video adapter Suitable for: Comments (Cat.

  • Page 104: Measuring, Counting And Comparing Techniques Using The Microscope

    • eyepiece crossline micrometer 10: 100, d = 26 mm An overview of stage micrometers and eyepiece reticles available from Carl Zeiss is given in chapter 1.6. Before the length measurement using the microscope can be performed, the micrometer or scale value of the used objective / eyepiece combination must be determined.

  • Page 105: Length Measurement Using The Digital Eyepiece Measurement Device

    Axiotech and Axiotech Carl Zeiss 3.7.2 Length measurement using the digital eyepiece measurement device The digital eyepiece measuring device is particularly suitable for measuring tasks requiring a higher accuracy than that obtained in comparison measurements using stage micrometers and crossline micrometers.
  • Page 106 Carl Zeiss Axiotech and Axiotech • Insert micrometer eyepiece with clamped integrated micrometer screw into the right tube and clamp it onto the tube using the clamping screw (3-35/9). • Focus the reticle figure by turning the eyelens on the micrometer eyepiece, then focus on the object.
  • Page 107 Axiotech and Axiotech Carl Zeiss 3.7.2.3 Measuring with the digital eyepiece measuring device Identical to measurements using eyepiece reticles (see section 3.7.1), the scale constant must also be calculated for the measurement of object sizes using the eyepiece measuring device. Only in the case of...

  • Page 108: Determination Of Microhardness Using The Mht-4 Microhardness Tester

    Carl Zeiss Axiotech and Axiotech • zero reset of the digital display at any point of the "0" measuring range, e.g. at the starting or end point of the measured distance, • measuring unit of the display in inch or mm "IN/M" with 1 inch = 25.4 mm.

  • Page 109: Determination Of Grain Sizes In Material Structures

    Axiotech and Axiotech Carl Zeiss 3.7.4 Determination of grain sizes in material structures 3.7.4.1 Measuring and counting technique The following reticles can be used to determine the grain parameters of standard and textured structures according to the line intersection technique: Eyepiece reticle 25/2 ×...
  • Page 110 Carl Zeiss Axiotech and Axiotech 3.7.4.2 Structure comparison reticles Three different structure comparison reticles according to ISO 643 -1983 and ASTM E 112 -88 can be used: Single grains (I) Single grains (I-n) Twin grains (II) grains without substructure...
  • Page 111 Axiotech and Axiotech Carl Zeiss 3.7.4.3 Determination of grain parameters or materials structures: • The grain size of the object imaged in the empty field must be compared to the grain sizes of the segments. • Determine the number of the sector, the grain size of which is nearly identical to that of the object image.
  • Page 112 Carl Zeiss Axiotech and Axiotech 3-46 B 40-020 e 06/99...

  • Page 113: Care, Maintenance, Troubleshooting And Service

    Axiotech and Axiotech Carl Zeiss CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Contents CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-3 Instrument care .......................4-3 Instrument maintenance ....................4-4 4.2.1 Performing checks ......................4-4 4.2.2 Changing the fuses......................4-4 4.2.3 Check instrument voltage and change it, if required ............4-5 Troubleshooting ......................4-6...
  • Page 114 Carl Zeiss Axiotech and Axiotech B 40-020 e 06/99...

  • Page 115: Instrument Care

    CARE; MAINTENANCE; TROUBLESHOOTING AND SERVICE Instrument care vario Maintenance of the Axiotech or Axiotech is limited to the following operations: • Switch off the instrument after every use, allow the illuminator(s) to cool down for approx. 15 min., and place instrument cover on the instrument to protect it from dust and humidity.

  • Page 116: Instrument Maintenance

    4.2.1 Performing checks • Make sure that the required line voltage is available, e.g. on the Axiotech 100 stand or the separate power unit SNT 12 V DC, 100 W. • Check the line cable and the plug for defects.

  • Page 117: Check Instrument Voltage And Change It, If Required

    Axiotech 100 microscope stand The stabilized 12 V, 100 W power unit is integrated in the Axiotech 100 microscope stand and operates in the two switchable line voltage ranges 100 V – 127 V AC or 220 ... 240 V AC. Before start-up, it is...

  • Page 118: Troubleshooting

    Carl Zeiss Axiotech and Axiotech Troubleshooting Problem Cause Remedy Vignetting or inhomogeneous The vis/phot pushrod on the Move the vis/phot pushrod in the image brightness in the field of camera tube is not in the correct correct (end) position, view;...
  • Page 119 Axiotech and Axiotech Carl Zeiss Problem Cause Remedy Image aberration Condenser not set correctly Set condenser correctly, see pp. 2-11 ff. and 3-22 ff. Nosepiece not correctly switched Correctly click-stop the nosepiece to click-stop position Specimen is not clamped on the...
  • Page 120 Carl Zeiss Axiotech and Axiotech Problem Reason Remedy The 12 V, 100 W halogen lamp Line cable not connected to the Connect line cable to the line and does not function, although line make sure to check the the On / Off switch is in "On"...

  • Page 121: Table Of Spares, Consumables And Tools

    000000-0127-019 protects power supply units from Axiotech 30: T 0.8 A; 250 V; 000000-0127-024 excessive load Axiotech 100: T 2.0 A/H; 250 V; 000000-0127-027 T 4.0 A/H; 250 V; Light filters: Interference wide-band filter green, d=32×4 467803-0000-000 for contrast enhancement in b/w photography and phase contrast Interference band filter green 546, d=32×3...

  • Page 122: Requesting Service

    Carl Zeiss Axiotech and Axiotech Requesting service All repairs of mechanical, optical and electronic components inside the instrument and of the Axiotech vario and Axiotech electronics system may only be performed by Carl Zeiss service staff or specially authorized personnel.
  • Page 123 Axiotech and Axiotech Carl Zeiss ANNEX List of Abbreviations........................A-3 Physical and Technical Units ......................A-4 Certification in Accordance with DIN EN ISO 9001/DIN EN 46001..........A-5 EC Conformity Declaration ......................A-7 B 40-020 e 06/99...
  • Page 124 Carl Zeiss Axiotech and Axiotech B 40-020 e 06/99...

  • Page 125: List Of Abbreviations

    Axiotech and Axiotech Carl Zeiss List of Abbreviations reflected light Alternating Current aspheric ASTM American Society of Testing Materials A-Plan achromatic objectives featuring improved image flatness (ICS line) suitable for eyeglass wearers Correction Blue (conversion filter) Charge Coupled Device...

  • Page 126: Physical And Technical Units

    Carl Zeiss Axiotech and Axiotech right (drive control on the right side of the mechanical stage) Single Lens Reflex Protection class wrench opening black-and-white slow-blow fuse type television T2-Adapter standardized adapter for 35 mm cameras Underwriters Laboratories magnification of the objective...

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