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ACCQPrep HP125
Section 4 Operation
4.3.6 Fraction Collection
with the AutoSampler
4-12
volume of the sample probe. Since the loop is still in the
separation position, the wash solvent is sent to waste.
10. The loop is switched to the load position.
11. The programmed injection volume is aspirated into the
sample loop. Once the programmed amount of sample is
aspirated into the probe, the probe is lifted and a small
amount of air is drawn into the sample probe. This mini-
mizes mixing of the sample with the bracketing solvent
which is aspirated next
12. The probe is dipped in the wash solvent to rinse the exte-
rior of the probe, then raised and shaken. This minimizes
sample contamination of the bracket solvent.
13. The probe moves to the bracket solvent sample container.
(0.05 mL of the bracket solvent is aspirated into the probe.
14. The probe is raised into the air and the fluid in the loop is
aspirated into the loop along with ~.05 mL of air to ensure
all off the sample and bracket solvent is loaded.
15. The loop is switched to the separate position.
16. The sample is now loaded and the separation will begin.
17. The next injection of the same sample is accomplished by
repeating the above process.
After the completion of the final injection of a sample, the system
washes the probe with the following sequence:
1. The inject valve will be moved to bypass to prevent
contamination of the loop during the cleaning process.
2. Air is drawn into the probe to eliminate any remaining
solvent in the probe.
3. The probe is placed into the wash station. The wash station
pump will flush wash fluid over the exterior of the probe
while 10 mL of wash fluid is drawn into the probe to wash
the interior flow path. The probe syringe pump will use
half strokes to improve the rinsing of any tiny amounts of
compound that may be present due to the wash process.
4. Once again, the probe is moved up and down over the wash
station to shake off any excess liquid and to prevent the
contamination of other samples or collection tubes.
5. Air is drawn into the probe to eliminate the strong wash
solvent from the sample flow path.
The fraction collection racks in the AutoSampler double the
fraction collection ability of the ACCQPrep. They are treated as
an extension of the racks present in the ACCQPrep. In general,
if racks are placed in both the ACCQPrep and AutoSampler,
available racks will used in the following order: ACCQPrep left
rack (A), ACCQPrep right rack (B), AutoSampler left rack (C),
AutoSampler right rack (D). If any racks are missing or iden-
tified as containing fractions from a previous separation, the
system moves to the next available rack in the order mentioned
above.
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Troubleshooting
