Hach Standard Method Procedure - Hach BODTrak II User Manual

BODTrak™ II procedures

5.4 Hach Standard Method procedure

Required apparatus:
Thermometer
BODTrak II bottle
Blender (optional)
Graduated cylinder
Tensette pipet and pipet tips
Wash water bottle
Required reagents:
2 nutrient buffer pillows
Additional nutrient or buffer (optional)
Deionized water
Before starting the test:
Use the sample volume table to choose the correct sample size
If seeding samples, prepare a seed blank before preparing a sample. Treat the seed blank
the same as any other sample and omit step 5.
Refer to section 5.7 on page 33
Table 6 Hach Standard Method sample volumes
BOD range
(mg/L)
0 to 35
0 to 70
0 to 350
0 to 700
Note: If seed strength is unknown, use 20 mL. Adjust seed volume as necessary to achieve
optimum results.
24
for special considerations.
Sample Seed volume
volume (mL)
370
305
110
45
Final volume
(mL)
10 to 35
10 to 35
10 to 35
10 to 35
(Table 6).
Dilution
(mL) factor
420 1.14
355 1.16
160 1.45
95 2.11