Certification Hach Company certifies this instrument was tested thoroughly, inspected and found to meet its published specifications when it was shipped from the factory. The BODTrak II has been tested and is certified as indicated to the following instrumentation standards:...
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Disconnect power from the BODTrak II instrument to verify the instrument is the source of the interference. • If the BODTrak II is plugged into the same outlet as the device with which it is interfering, try another outlet. •...
Section 2 General information 2.1 Safety information Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment. Make sure that the protection provided by this equipment is not impaired, do not use or install this equipment in any manner other than that specified in this manual.
Degassing can cause negative errors when heat is applied to a sample to achieve experimental temperature. The BODTrak II adjusts for this occurrence. The BODTrak II does not start the test until the temperature gets to equilibrium. 2.2.1 Oxygen transfer to sample Bacteria in the sample use oxygen while consuming organic matter in the sample bottles.
2.2.2 Pressure sensor function The BODTrak II is sealed to prevent external atmospheric pressure changes in the test bottle. Pressure sensors monitor air pressure in the sample bottles. When oxygen is consumed, the pressure in the bottle head space drops. The pressure drop correlates directly to BOD.
A 230 VAC harmonized power cord with a continental European plug • Power supply, auto–switching between 115 V and 230 V • 6 seal cups • 6 BODTrak II amber sample bottles • 6 BODTrak II magnetic stir bars • Spatula scoop •...
Installation 3.2 Electrical installation The power adaptor supplies AC power to the IEC universal connector (Figure The power switch powers the instrument on and off. Figure 1 External connections 1 Power switch 3 RS232 connector 2 IEC universal connector...
Section 4 Operation 4.1 Operational controls The BODTrak™ II operator controls are shown in Figure Figure 2 Operational controls 1 Display keys ON/OFF 2 Channel selection keys 5 Power indicator 3 Arrow keys The ON/OFF keys start and stop a test. They do not power the instrument on and off.
Operation 4.1.1 Channel selection keys Push the related channel selection key to show data for one of the 6 bottles. The channel selection keys are also used in the instrument setup menu to choose a parameter to be edited (Table Table 3 Channel key setup parameters Channel Parameter...
The BODTrak II instrument is equipped as Data Communication Equipment (DCE). The BODTrak II operates at 9600 baud with 8 data bits, no parity and one stop bit. The computer or printer will not receive complete transmissions if the device cannot continuously receive at 9600 baud.
Name the file and click SAVE In the Capture Text window click START Power on the BODTrak II. Push the applicable channel key for the data to be downloaded. 10. Type GA in the HyperTerminal window, then push . The transfer is ENTER complete when the screen stops adding new data.
Operation 4.5.2 Data format When a result array is downloaded to HyperTerminal, all data from the test are sent without pause. The data flow cannot be stopped or paused. Figure 3 shows channel number, start date, start time, and the format of the downloaded data.
5 seconds at any time during a test. This moves the test results from the BODTrak II to the printer. The instrument will send a copy of the graphical display and a truncated data stream (127 data points).
0 to 25 mg/L BOD. Adjustment is not necessary for other test ranges. Refer to section 5.7 on page 33 for special considerations including sample seeding and pretreatment. Use only BODTrak II stir bars and bottles. They are designed specifically for use with the BODTrak II.
BODTrak™ II procedures 5.2 Simplified procedure Required apparatus: BODTrak II bottle Thermometer Blender (optional) Graduated cylinder Required reagents: 2 nutrient buffer pillows Table 4 Simplified sample volumes BOD range mg/L Sample volume mL 0 to 35 0 to 70 0 to 350 0 to 700 1.
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5. Transfer the contents of 6. Continue to the nutrient buffer pillows to the graduated cylinder to completion steps for all the graduated cylinder. a BODTrak II bottle. procedures (section 5.5 Repeat steps 1 to 5 for on page 27).
On a standard containing 150 mg/L each of glucose and glutamic acid, a single analyst using 6 BODTrak II instruments and testing 44 samples obtained a mean of 235 mg/L BOD with a 95% Confidence Limit of Distribution of 11 mg/L or a range of 224 to 246 mg/L BOD after 5 days.
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1. Add approximately 2. Use a volumetric pipet 3. Add the contents of 2 30 mL of deionized water to transfer 8.0 mL of Hach nutrient buffer pillows to to a 200 mL graduated GGA solution to the the graduated cylinder.
If seeding samples, prepare a seed blank before preparing a sample. Treat the seed blank the same as any other sample and omit step 5. Refer to section 5.7 on page 33 for special considerations. Table 6 Hach Standard Method sample volumes BOD range Sample Seed volume Final volume...
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BODTrak™ II procedures 1. Heat or cool the 2. Homogenize the 3. Choose the correct sample to 19 to 21 ºC sample in a blender if it sample size for the (66 to 70 ºF). contains large settleable sample range (Table 6 on or floatable solids.
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(section 5.5 deionized wash water BODTrak II bottle. on page 27). bottle (Table 6 on page Note: Repeat steps 1 to 8 for additional samples.
BOD incubator Seal cup Stir bar Required reagents: 2 potassium hydroxide pellets 1. Put a BODTrak II stir 2. Put a seal cup into the 3. Use a spatula scoop bar into the bottle. neck of the bottle. to add 2 potassium hydroxide pellets to the seal cup.
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BODTrak™ II procedures 4. Put the bottles on the 5. Put the instrument in 6. Plug in and power on BODTrak II chassis. the incubator. The the instrument. Make Connect the applicable incubator temperature sure all stir bars are tube to the sample bottle must be 20 ±...
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BODTrak™ II procedures 10.To start the test, push 11.Push the key. The 12.Use the arrow keys to the channel number range selection menu is choose the test range. applicable to the bottle. shown. Note: Use the left arrow key for the 0 to 35 and 0 to 70 mg/L ranges.
Hach Standard Method. 5.5.1.1 Simplified sample results The Simplified Procedure results are shown on the BODTrak II display. Push the applicable channel selection key to show the results. Note: If the sample was pre-diluted, apply a dilution factor to the instrument reading (section 5.7.1 on page...
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BODTrak™ II procedures When samples are seeded, calculate the results using this equation and the corrected results: ⎛ ⎞ ------- - BOD mg/L – ⎝ ⎠ Where: A = corrected BOD of the seeded sample B = corrected BOD of the seed blank SA = volume of seed in sample (sample can also be GGA) SB = volume of seed in seed blank Example:...
BODTrak™ II procedures 5.6 Typical curves Typical curves through a 10 day test period are shown in Figure 4. For incorrect curves refer to Figure 5 on page Figure 4 Typical curves 1 Typical with substrate variation 3 Typical with time lag 2 Typical...
Standard Methods for the Examination of Water and Wastewater, 21st Ed., 2005, 5210 D recommends an incubation temperature of 20 ±1 °C (68 °F) for the BOD test. Put the BODTrak II instrument in an incubator that is adjusted to 20 ±1 °C. An applicable BOD incubator is available from Hach (section 8.1 on page...
BODTrak™ II procedures 5.7.5 Chlorine Any chlorine in the sample must be removed prior to testing. Keep the sample at room temperature for 1 to 2 hours before a test to dissipate low chlorine concentrations. If any chlorine remains after sitting for 2 hours or if the chlorine concentration is high, add sodium thiosulfate to remove the chlorine: In a 250 mL erlenmeyer flask add 100-mL of sample.
Section 6 Maintenance DANGER Only qualified personnel should conduct the tasks described in this section of the manual. 6.1 Cleaning the instrument Clean spills on the BODTrak™ II instrument with a soft cloth which has been dampened with deionized or distilled water. 6.1.1 Sample bottles After each test, empty the sample bottles and flush them thoroughly with hot water.
Section 7 Troubleshooting Incorrect BOD curves through a 10 day test period are shown in Figure 5. For typical curves refer to Figure 4 on page Figure 5 Incorrect BOD curves 1 High oxygen demand 4 Initial sample temperature below º...
Control nitrification problems with Hach Nitrification Inhibitor. Dispense the inhibitor powder into an empty sample bottle and then add the sample. With the Hach Dispenser cap, dispense 6 shots (approximately 0.48 grams) into the empty bottle. Refer to replacement parts and...
Troubleshooting 7.5 Bottle leak Curve 5 (Figure 5 on page 37) shows a bottle leak. A bottle leak can also cause no response from the system. If such a response occurs, check the seal cup and bottle cap for contamination or damage.
8.1 Replacement parts Description Quantity Item Number BODTrak™ Instrument, 115/230 VAC 2952400 Bottle, BODTrak II, amber (6x) 714421 Power cord, 18/3 SVT 7.5’, 10A-125 VAC for North 2959200 American 115 VAC use Power Cord, 8’, with continental European plug for 230...
8.3 Optional reagents (continued) Description Quantity Item Number Sulfuric acid, 1.0 N standard solution, 127053 1000 mL Voluette ampule standard for BOD, 3000 mg/L for 1486610 manometric, 10-mL/ampule, 16 ampules 8.4 Accessories Description Quantity Item Number Ampule breaker kit for voluette ampules 2196800 Bottle, wash, 500 mL 62011...
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Replacement parts and accessories 8.4 Accessories (continued) Description Quantity Item Number Thermometer, Mercury, -20 to 110 °C 56601 Thermometer, non-Mercury, -20 to 110 °C 2635702 Water Still, 120 V 2615900 Water Still, 220 V 2615902 Water System, Ultrapure, Millipore Direct -Q 3 2512100 DQ3 purification pack 2512201...
HACH Company World Repair Service in the Repair Service in Headquarters United States: Canada: P.O. Box 389 HACH Company Hach Sales & Service Loveland, Colorado Ames Service Canada Ltd. 80539-0389 U.S.A. 100 Dayton Avenue 1313 Border Street, Unit 34 Tel (800) 227-HACH...
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Contact Information HACH LANGE S.L.U. HACH LANGE LDA HACH LANGE SP.ZO.O. Edif. Arteaga Centrum Av. do Forte nº8 ul. Opolska 143 a C/Larrauri, 1C- 2ª Pl. Fracção M PL-52-013 Wrocław E-48160 Derio/Vizcaya P-2790-072 Carnaxide Tel. +48 (0)71 342 10-83 Tel. +34 94 657 33 88 Tel.