Applied Biosystems QuantStudio 3D Digital PCR Manuals

Manuals and User Guides for Applied Biosystems QuantStudio 3D Digital PCR. We have 1 Applied Biosystems QuantStudio 3D Digital PCR manual available for free PDF download: User Manual

Applied Biosystems QuantStudio 3D Digital PCR User Manual (128 pages)

for use with: QuantStudio 3D Digital PCR Instrument ProFlex 2x Flat PCR System or Dual Flat Block GeneAmp PCR System 9700 QuantStudio 3D Digital PCR Chip v2 or QuantStudio 3D Digital PCR Chip QuantStudio 3D Digital PCR Master Mix v2 or QuantStudio 3D Digi

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 4 MB

Table of Contents
3
About this Guide
8
Revision History
8
Purpose
9
Prerequisites
9
CHAPTER 1 Product Information
10
About the Quantstudio ™ 3D Digital PCR System
10
Instruments, Kits, Consumables, and Accessories
11
Chapter 1 Product Information Materials Required but Not Provided
13
Materials Required but Not Provided
13
Power Line Regulator
14
Surge Protector
14
About the Instrument
14
Data Collection
15
Supported Dyes
15
About the Software
15
System Requirements
15
About the Chips
16
CHAPTER 2 Getting Started
17
Operational Workflow
17
Connect the Quantstudio ™ 3D Instrument to a Network
18
Connect the Quantstudio 3D Analysissuite
18
Software
18
Choosing a Thermal Cycler
18
Compatible Thermal Cyclers
18
Chapter 2 Getting Started Choosing a Thermal Cycler
19
Guidelines for Loading and Sealing Chips
20
Prepare the DNA Samples
21
Quality of DNA
21
Quantity of DNA
21
Quantitation Methods
22
Sample Dilution
22
Determine the Optimal Dilution When the Target Is Known
22
Determine the Optimal Dilution When the Target Is Unknown
23
Prepare the Digital PCR Reactions
23
Required Materials
23
Guidelines for Dpcr Sample Preparation
23
Chapter 3 Prepare the Digital PCR Reaction and Load the Chips Prepare the Digital PCR Reactions
24
Recommended DNA Volume and Concentration
24
Prepare the Dpcr Reaction MIX
24
Load the Chips Using the Quantstudio ™ 3D Digital PCR Chip Loader
25
Materials Required
25
Chip Loader Status Light
26
Prepare the Chip Loading Workspace
26
Prepare the Chip Sealant (V1 Chip Lids Only)
27
Prepare the Syringe Containing the Immersion Fluid
27
Load and Seal the Chips
28
Fill the Chip Case with Immersion Fluid
32
Seal the Chip Case with the Label (V2 Chip Lids Only)
33
Seal the Chip Case with Chip Sealant (V1 Chip Lids Only)
34
CHAPTER 4 Thermal Cycle the Chips
36
Materials Required
36
Prepare the Thermal Cycler
36
Guidelines for Handling Loaded Chips
39
Perform the PCR
39
Chapter 4 Thermal Cycle the Chips Perform the PCR
40
Unload the Thermal Cycler
42
CHAPTER 5 Image and Analyze the Chips
44
About Imaging and Primary Analysis
44
Quantstudio ™ 3D Digital PCR System Data Flow
44
About the Instrument Interface
45
Chip Imaging
46
Materials Required
46
Guidelines for Imaging Multiple Chips
46
Specify the Chip Well Volume
46
Specify the Data Destination
47
Image the Chips
48
Primary Analysis
51
About Imaging Data and Primary Analysis
51
About the Results Screen
51
Chapter 5 Image and Analyze the Chips Primary Analysis
52
Data Quality Flags
53
About Data Files
53
Transfer the Results
54
Reanalyze Well Volumes
55
Using the Quantstudio ™ 3D Analysissuite ™ Software
56
Access the Software from a Cloud Account
57
Obtain Information from the Help System
57
CHAPTER 6 Troubleshooting
58
Troubleshooting Chip Images Using the Chip View
58
APPENDIX A Install the Quantstudio ™ 3D Digital PCR System
65
Time Required for Installation
65
Materials Required
65
Before You Begin
65
Appendix A Install the Quantstudio Plan the Laboratory Layout
66
Plan the Laboratory Layout
66
Choose the Instrument Locations
66
Install the Quantstudio ™ 3D Digital PCR Instrument
67
Install the Proflex ™ 2X Flat PCR System
72
Install the Geneamp
78
PCR System 9700
78
APPENDIX B Specifications and Layout
85
Component Dimensions and Weights
85
Appendix B Specifications and Layout Quantstudio ™ 3D Digital PCR Instrument Layout and Connections
86
Instrument Clearances
86
Electrical Specifications
87
Environmental Requirements
88
Network Requirements
89
APPENDIX C Maintenance
90
Cleaning the Chip Tray and Sample Block
90
Materials Required
90
Appendix C Maintenance Cleaning the Chip Tray and Sample Block
91
Clean the Chip Tray or Sample Block
91
Configure the Instrument Settings
92
(Optional) Set the Instrument Name
92
Set the Date and Time
93
Check the Instrument Firmware
93
Update the Instrument Firmware
94
Returning Your Instrument for Service
95
Replace the Instrument Fuses
96
Materials Required
96
Replace the Fuses
96
Calibrate the Touchscreen
97
View the Instrument Log
98
APPENDIX D Networking
99
Networking Overview
99
About the Network Port and Wireless Adaptor
99
Appendix D Networking Connect the Instrument to the Network
100
Networking Guidelines and Best Practices
100
Connect the Instrument to the Network
100
Network Setup Workflow
100
Materials Required
101
Required Network Information
101
Connect the Quantstudio ™ 3D Instrument to a Network
102
Create a Network Destination to Receive Run Data
107
APPENDIX E Load Chips Manually
110
Guidelines for Loading and Sealing Chips
110
Appendix E Load Chips Manually Materials Required
111
Materials Required
111
Prepare the Chip Sealant (V1 Chip Lids Only)
112
Prepare the Syringe Containing the Immersion Fluid
112
Load and Seal the Chips Manually
113
Fill the Chip Case with Immersion Fluid
116
Seal the Chip Case with the Label (V2 Chip Lids Only)
117
Seal the Chip Case with Sealant Using the UV Stylus (V1 Chip Lids Only)
118
APPENDIX F Safety
120
Symbols on this Instrument
120
Conformity Symbols
121
Safety Alerts on this Instrument
122
Location of Safety Alerts on the Instrument
122
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
123
Instrument Safety
123
General
123
Electrical
123
Cleaning and Decontamination
124
Safety and Electromagnetic Compatibility (EMC) Standards
124
Safety
124
Emc
124
Appendix F Safety Chemical Safety
125
Environmental Design
125
Chemical Safety
125
Biological Hazard Safety
126
Documentation and Support
127
Customer and Technical Support
127
Limited Product Warranty
127

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Applied Biosystems QuantStudio 3D Digital PCR User Manual (128 pages)

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