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WPI INV-101 Install Manual

Inverted microscope
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INV-101
Inverted Microscope
INSTALL GUIDE
Serial No._____________________
102811
World Precision Instruments

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Summary of Contents for WPI INV-101

  • Page 1 INV-101 Inverted Microscope INSTALL GUIDE Serial No._____________________ 102811 World Precision Instruments...
  • Page 2 INV-101 WORLD PRECISION INSTRUMENTS...

  • Page 3: Table Of Contents

    INV-101 CONTENTS ABOUT THIS MANUAL ..........................1 INTRODUCTION ............................2 Unpacking ..............................2 MICROSCOPE SETUP ........................... 3 Setting up the Base and Stage ......................3 Installing the Condenser and Lamp Housing ................7 Setting up the Stage and Phase Aperture ..................9 Installing the Trinocular Head Assembly ..................
  • Page 4 INV-101 WORLD PRECISION INSTRUMENTS...

  • Page 5: About This Manual

    Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1–INV-101 Inverted microscope WORLD PRECISION INSTRUMENTS...

  • Page 6: Introduction

    Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 28 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com.

  • Page 7: Microscope Setup

    The following instructions graphically show how to setup your inverted microscope. NOTE: If you have a problem/issue that falls outside the definitions of this manual, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc. com. Setting up the Base and Stage Remove the base of the microscope and set it up on a firm tabletop surface (Fig.
  • Page 8 INV-101 Fig. 4–Back of the microscope base showing power connection, serial number and fuse compartment Fig. 5–Power switch and lamp brightness control on the side of the microscope Locate the coaxial stage assembly. It is shown in Fig. 6. The captive mounting screws are located on the side of it (Fig.
  • Page 9 INV-101 Fig. 7–Captive mounting screws on the coaxial stage assembly Tip the microscope base over on its left side to reveal the location of the screw mounts. Then, position the coaxial stage (Fig.8). Fig. 9 shows a close up of the captive screws.
  • Page 10 INV-101 Locate the left extension stage plate bottom shown in Fig. 11 (bottom view) and Fig. 12 (top view). Fig. 11- Extension stage plate (bottom view) Fig. 12–Extension stage plate (top view) Push extension arm of the coaxial stage assembly inward so that you do not damage it when you mount the left extension stage plate (Fig.

  • Page 11: Installing The Condenser And Lamp Housing

    INV-101 Turn the microscope upright (Fig. 16). Fig. 16–Coaxial and left stage plate mounted Installing the Condenser and Lamp Housing Locate the condenser assembly and lamp housing (Fig. 17). Condenser Assembly Lamp Housing Fig. 17–Condenser assembly and lamp housing Lower the condenser assembly into the condenser mount (Fig. 18). It should slide easily into place.
  • Page 12 INV-101 Tighten hex screws to secure the condenser assembly into the condenser mount (Fig. 20). Fig. 20–Tighten hex screws on condenser head Plug the lamp housing into the lamp connector (Fig. 21). Fig. 21–Connect the lamp Fig. 22–Lamp connected Fig. 23–Install lamp Lower the lamp housing onto the mounting pin sockets in the top of the condenser head.

  • Page 13: Setting Up The Stage And Phase Aperture

    INV-101 Setting up the Stage and Phase Aperture Position the clear stage plate in the recess on the stage (Fig. 25). Fig. 25–Clear stage plate, installed Install the phase aperture frame (Fig. 26) in the slot on the condenser head from the right side.
  • Page 14 INV-101 Place the trinocular head on the head mount and tighten the side screw (Fig. 30). Fig. 30–Position head on the mount Locate the threaded C-mount, eye tube mount and trinocular extension tube (Fig. 31). Trinocular Extension Tube Eye Tube...

  • Page 15: Installing The Objectives

    INV-101 Depending on your application, either the eye tube mount or the C-mount can be installed. Choose one of the two options. • Mount the eye tube mount by inserting it into the extension tube (Fig. 34). • Mount the threaded C-mount by screwing it into the extension tube (Fig. 35).
  • Page 16 INV-101 Remove a single dust cover from the nosepiece (Fig. 38). When it is removed, an opening is revealed (Fig. 39). For ease of installation, that opening should point straight up through the opening on the microscope stage. Fig. 38–Removing dust cover to mount objective Fig. 39–Position for mounting objective Remove the desired objective from its shipping case (Fig.

  • Page 17: Installing Adapter Plates, Eyepieces And Filters

    INV-101 Rotate the nosepiece to access next mounting location (Fig. 43). Fig. 43–Rotated nosepiece Fig. 44–Four objectives mounted Repeat steps 1-4 to install the remaining objectives. Do not remove the dust covers until you are ready to install the next objective. This prevents any stray dust from be- ing trapped on the objective.
  • Page 18 INV-101 Insert the 35mm adapter plate into the rectangular plate (Fig. 47). Fig. 47–Assembled rectangular plate and 35mm insert Place the rectangular plate on the stage plate (Fig 48). Fig. 48–Placement location of the assembled stage plate Remove the eyepieces from the dust wraps (Fig. 49) and remove the dust covers from the eyepiece openings on the trinocular head (Fig.
  • Page 19 INV-101 Place the eyepieces in the microscope ocular openings (Fig 51). Adjust the diopter set- ting to zero. Fig. 51–Placement of eyepieces Locate the filters (Fig. 52). Fig. 52–Filters Remove the filter holder from the condenser head as shown in Fig. 53 and Fig. 54.
  • Page 20 INV-101 Place the desired filter in the filter holder (Fig. 55) and slide the holder back into the condenser head (Fig. 56 and 57). Fig. 55–Insert filter Fig. 56–Position holder Fig. 57–Slide holder in place TIP: If desired, the filter may alternatively be placed in the condenser annulus slider (Fig.

  • Page 21: Phase Contrast Alignment

    INV-101 Phase Contrast Alignment There are two phase contrast objectives included with the INV-101 microscope, the 10X and the 20X. On a simplified level, phase contrast imaging occurs by the interference of light that is created by special masks in the light path. There are two masks, each located in a different part of the light path, which must be positioned correctly relative to each another to create the phase contrast effect.
  • Page 22 INV-101 View the phase ring through the phase telescope. Adjust the focus on the phase telescope for a sharp image of the phase ring. The phase telescope focus is adjusted by screwing it in or out (Fig. 62). Fig. 62–Focus adjustment Slide the phase aperture fame into position with the 10X/20X condenser annulus in the condenser light path.

  • Page 23: Mounting A Camera

    Fig. 67–Annulus alignment Mounting a Camera A camera is not included with the microscope, but WPI carries a variety of cameras if one is necessary. In the example shown below the COLCAM closed circuit television (CCTV) camera is used. This camera can be connected to a television. Digital cameras are available that will connect with a computer or laptop.

  • Page 24: Direct C-Mount On A Threaded Triangular Attachment

    INV-101 Direct C-Mount on a Threaded Triangular Attachment Position the camera over the 1X threaded C-mount (Fig. 68) and screw it into position (Fig. 69). Upper Set Screw Fig. 68–Position camera Fig. 69–Screw into place Adjust an microscope for par focalization: •...

  • Page 25: Vertical Eye Tube Mounting

    INV-101 Vertical Eye Tube Mounting This option requires the use of the eyepiece lens from the eyepiece adapter kit (WPI #503097). It allows the camera to be mounted on the eyepiece tube. Install the eyepiece lens on the camera and position it over the vertical (trinocular) eyepiece tube (Fig.

  • Page 26: Eyepiece Plus C-Mount Mounting

    Eyepiece Plus C-Mount Mounting This option requires the use of the eyepiece lens, +30mm adapter and female C-mount of the eyepiece adapter kit (WPI #503097). Then, the camera can be mounted on the 1X C-mount. Install the eyepiece lens on the camera, slide it into the +30mm adapter (Fig. 74) Install the threaded female C-mount on the eyepiece tube 1X C-mount (Fig.

  • Page 27: Basic Microscope Operations

    INV-101 BASIC MICROSCOPE OPERATIONS Camera Shutter (Optical) Path To enable viewing through the trinocular extension tube, pull out (open position) the handle shown in Fig. 77. When this switch is in the closed position, nothing can be viewed through the trinocular extension tube. When no camera is mounted, this switch should be in the closed position to prevent excessive light entering the system.

  • Page 28: Micrometer Focus Control

    INV-101 Micrometer Focus Control The knob shown in Fig. 79 adjusts the height of the microscope stage and is used for coarse and fine focusing of the microscope. Rotate the entire knob for coarse adjustment and the inner dial for fine adjustment.
  • Page 29 INV-101 Locate the tiny notch on the back side of the tension knob and insert the tip of the tension adjustment tool in the notch (Fig. 81). Rotate the tool clockwise to tighten the tension or counter-clockwise to loosen it (Fig.
  • Page 30 INV-101 WORLD PRECISION INSTRUMENTS...

  • Page 31: Index

    INV-101 INDEX Symbols nosepiece 12 35mm insert 13 100mm round stage mount 13 503097 19, 21, 22 objectives 11 bulb 24 par focalization 20 phase aperture plate 9 camera 19 power 4 CCTV 19 C-mount 10, 20, 22 rectangular plate 13...

  • Page 32: Warranty

    WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product.

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