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Summary of Contents for WPI PIMT201
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL PIMT201 Inverted Microscope Serial No._____________________ www.wpiinc.com 022321...
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Page 3: Table Of Contents
PIMT201 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Unpacking ........................... 2 MICROSCOPE SETUP ........................3 Setting up the Base and Stage ....................3 Installing the Condenser and Lamp Housing ..............7 Setting up the Stage and Phase Aperture ................10 Installing the Trinocular Head Assembly ................10... - Page 4 World Precision Instruments...
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Page 5: About This Manual
Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—PIMT201 Inverted microscope World Precision Instruments... -
Page 6: Introduction
INTRODUCTION This document details the assembly of the PIMT201 inverted microscope. It includes optional installation of a camera. All cameras and mounting adapters are sold separately. Unpacking Upon receipt of this instrument, make a thorough inspection of the contents and check for possible damage. -
Page 7: Microscope Setup
The following instructions graphically show how to setup your inverted microscope. NOTE: If you have a problem/issue that falls outside the definitions of this manual, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc. com. Setting up the Base and Stage Remove the base of the microscope and set it up on a firm tabletop surface (Fig. - Page 8 Fig. 4—Back of the microscope base showing power connection, serial number and fuse compartment Fig. 5—Power switch and lamp brightness control on the side of the microscope Locate the coaxial stage assembly. It is shown in Fig. 6. The captive mounting screws are located on the side of it (Fig.
- Page 9 PIMT201 Fig. 7—Captive mounting screws on the coaxial stage assembly Tip the microscope base over on its left side to reveal the location of the screw mounts. Then, position the coaxial stage (Fig.8). Fig. 9 shows a close up of the captive screws.
- Page 10 Locate the left extension stage plate bottom shown in Fig. 11 (bottom view) and Fig. 12 (top view). Fig. 11—Extension stage plate (bottom view) Fig. 12—Extension stage plate (top view) Push extension arm of the coaxial stage assembly inward so that you do not damage it when you mount the left extension stage plate (Fig.
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Page 11: Installing The Condenser And Lamp Housing
PIMT201 Turn the microscope upright (Fig. 16). Fig. 16—Coaxial and left stage plate mounted Installing the Condenser and Lamp Housing Locate the condenser assembly and lamp housing (Fig. 17). Condenser Assembly Lamp Housing Fig. 17—Condenser assembly and lamp housing Lower the condenser assembly into the condenser mount (Fig. 18). It should slide easily into place. - Page 12 Fig. 18—Condenser in mounting position Fig. 19—Condenser rotated 90º CCW Tighten hex screws to secure the condenser assembly into the condenser mount (Fig. 20). Fig. 20—Tighten hex screws on condenser head World Precision Instruments...
- Page 13 PIMT201 Plug the lamp housing into the lamp connector (Fig. 21). Fig. 21—Connect the lamp Fig. 22—Lamp connected Fig. 23—Install lamp Lower the lamp housing onto the mounting pin sockets in the top of the condenser head. (Fig. 23). The assembled condenser and lamp housing is shown in Fig.
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Page 14: Setting Up The Stage And Phase Aperture
Setting up the Stage and Phase Aperture Position the clear stage plate in the recess on the stage (Fig. 25). Fig. 25—Clear stage plate, installed Install the phase aperture frame (Fig. 26) in the slot on the condenser head from the right side. - Page 15 PIMT201 Place the trinocular head on the head mount and tighten the side screw (Fig. 30). Fig. 30—Position head on the mount Locate the threaded C-mount, eye tube mount and trinocular extension tube (Fig. 31). Trinocular Extension Tube Eye Tube...
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Page 16: Installing The Objectives
Depending on your application, either the eye tube mount or the C-mount can be installed. Choose one of the two options. • Mount the eye tube mount by inserting it into the extension tube (Fig. 34). • Mount the threaded C-mount by screwing it into the extension tube (Fig. 35). Fig. - Page 17 PIMT201 Remove a single dust cover from the nosepiece (Fig. 38). When it is removed, an opening is revealed (Fig. 39). For ease of installation, that opening should point straight up through the opening on the microscope stage. Fig. 38—Removing dust cover to mount objective Fig.
- Page 18 Rotate the nosepiece to access next mounting location (Fig. 43). Fig. 43—Rotated nosepiece Fig. 44—Four objectives mounted Repeat steps 1-4 to install the remaining objectives. Do not remove the dust covers until you are ready to install the next objective. This prevents any stray dust from being trapped on the objective.
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Page 19: Installing Adapter Plates, Eyepieces And Filters
PIMT201 Installing Adapter Plates, Eyepieces and Filters Locate the 100mm round stage mount, rectangular plate and 35mm insert (Fig. 46). 50mm/1x3” Slide Rectangular Adapter Lab Jar Adapter 35mm Dish Adapter Fig. 46—100mm round stage mount, rectangular plate and 35mm insert Insert the 35mm adapter plate into the rectangular plate (Fig. - Page 20 Remove the eyepieces from the dust wraps (Fig. 49) and remove the dust covers from the eyepiece openings on the trinocular head (Fig. 50). Fig. 49—Eyepieces Fig. 50—Remove dust covers from trinocular head Place the eyepieces in the microscope ocular openings (Fig 51). Adjust the diopter setting to zero.
- Page 21 PIMT201 Locate the filters (Fig. 52). Fig. 52—Filters Remove the filter holder from the condenser head as shown in Fig. 53 and Fig. Fig. 53—Filter holder position Fig. 54—Filter holder removed Place the desired filter in the filter holder (Fig. 55) and slide the holder back into the condenser head (Fig.
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Page 22: Phase Contrast Alignment
Fig. 59—Condenser annulus slider in position Phase Contrast Alignment There are two phase contrast objectives included with the PIMT201 microscope, the 10X and the 20X. On a simplified level, phase contrast imaging occurs by the interference of light that is created by special masks in the light path. There are two masks, each located in a different part of the light path, which must be positioned correctly relative to each another to create the phase contrast effect. -
Page 23: Adjusting The Phase Contrast Alignment
PIMT201 Notice that there are two condenser annulus masks on the phase aperture frame. (See Fig. 26, page 10.) One is for use with the 10X and 20X phase contrast objectives, and the larger one is for a 40X phase contrast objective (not included). - Page 24 Slide the phase aperture fame into position with the 10X/20X condenser annulus in the condenser light path. (Fig. 63) NOTE: If you are using a 40X phase contrast objective (not included), use the 40X condenser annulus on the phase aperture frame. Fig.
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Page 25: Mounting A Camera
Fig. 67—Annulus alignment Mounting a Camera A camera is not included with the microscope, but WPI carries a variety of cameras if one is necessary. In the example shown below the COLCAM closed circuit television (CCTV) camera is used. This camera can be connected to a television. Digital cameras are available that will connect with a computer or laptop. -
Page 26: Vertical Eye Tube Mounting
Once the rotation is set, lock the lower screw. Vertical Eye Tube Mounting This option requires the use of the eyepiece lens from the eyepiece adapter kit (WPI #503097). It allows the camera to be mounted on the eyepiece tube. -
Page 27: Eyepiece Plus C-Mount Mounting
Eyepiece Plus C-Mount Mounting This option requires the use of the eyepiece lens, +30mm adapter and female C-mount of the eyepiece adapter kit (WPI #503097). Then, the camera can be mounted on the 1X C-mount. Install the eyepiece lens on the camera, slide it into the +30mm adapter (Fig. 74) Install the threaded female C-mount on the eyepiece tube 1X C-mount (Fig. -
Page 28: Basic Microscope Operations
Position the camera over the eyepiece tube and slide the camera unit into place. When properly assembled, it looks like Fig. 76. Fig. 76— Camera in position BASIC MICROSCOPE OPERATIONS Camera Shutter (Optical) Path To enable viewing through the trinocular extension tube, pull out (open position) the handle shown in Fig. -
Page 29: Coaxial X-Y Control
PIMT201 Coaxial X-Y Control The knob shown in Fig. 78 controls the x and y movement of the microscope stage. Fig. 78—Coaxial X-Y Control Micrometer Focus Control The knob shown in Fig. 79 adjusts the height of the microscope stage and is used for coarse and fine focusing of the microscope. -
Page 30: Focus Tensioning Adjustments
Focus Tensioning Adjustments The tension on the focus knob can be adjusted (if desired) by using the tension adjustment tool that came in the spare parts and tool kit with the microscope. NOTE: The phase telescope comes with spare fuses, a spare bulb and a tool kit (Fig. 80). -
Page 31: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 32 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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