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Summary of Contents for WPI MF200
- Page 1 WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL MF200 Microforge with 40× long working distance objective Serial No._____________________ www.wpiinc.com 030716...
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Page 3: Table Of Contents
Mounting the Filament Adjustment Assembly to the Microscope ......9 Positioning the Micropipette Holder ................10 Mounting the Heating Filament ..................10 Basic Operations for Using the MF200 ................11 Applications ..........................14 Fire Polishing the Patch Clamp Pipette ................14 Fire Polishing a Pipette Tip .....................15 Tip Size Reduction ......................16... - Page 4 World Precision Instruments...
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Page 5: About This Manual
Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—MF200 System World Precision Instruments... -
Page 6: Introduction
INTRODUCTION The MF200 Microforge is a versatile device designed specifically for the fabrication of glass micropipettes and other related tools. Originally designed by Dr. Ming Li of the Department of Pharmacology, University of South Alabama, it has been extensively improved to provide greater accuracy and ease of use. It is simple, durable and reliable. -
Page 7: Instrument Description
Spacer Ring for mounting on the 10X objective with 22mm OD (1) 75027 Spacer Ring for mounting on the 10X objective with 21mm OD NOTE: The spacer ring (WPI #300497 may not be necessary for objectives with larger outside diameters. It is use with objectives smaller than 23.0mm. Unpacking Upon receipt of this instrument, make a thorough inspection of the contents and check for possible damage. -
Page 8: Description
See page 19 for details. Optional accessories (including a 25X LWD objective for the W30S microscope) further expand the MF200 system functionality. Fig. 3— MF200 System (not shown: 12 V DC power converter for Microforge Control Unit). World Precision Instruments... -
Page 9: Positioning And Focus
Low heat capacity and low thermal expansion of the filaments are key design features of the MF200 Microforge. The low heat capacity of the filament allows it to reach fire-polishing temperatures without excessive heat. This permits the pipette tip to be brought close to the filament during polishing without fear of collapsing the pipette tip and eliminates the need for an auxiliary air cooling system. -
Page 10: Microscope
W30S 10X objective. Power Controller (Control Unit) The MF200 is powered by a 12VDC adapter to supply power to the Control Unit. The Control Unit is compact and lightweight, and its output power is electrically stable and reproducible. - Page 11 MF200 push-button polish switch on the Control Unit turns the heating filament on and off. An optional foot switch is available for complex fire polishing. Use of the optional foot switch leaves the hands free to move the pipette and control the variable heat adjustment on the Control Unit.
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Page 12: Operating Instructions
OPERATING INSTRUCTIONS Setting up the MF200 Four steps are required to set up the MF200. First, mount the LWD to the microscope. Then, mount the Filament Adjustment Assembly to the microscope. Next, position the micropipette holder on the microscope stage. Finally, install the heating filament. -
Page 13: Mounting The Filament Adjustment Assembly To The Microscope
MF200 Mounting the Filament Adjustment Assembly to the Microscope Using the 3/16 hex wrench provided, loosen the mounting screw on the Filament Adjustment Assembly to open the ring so that it will fit comfortably over the objective and the Spacing Ring (Fig. 7). -
Page 14: Positioning The Micropipette Holder
Connect the appropriate plug from the AC/DC converter into the 12VDC receptacle on the Microforge Control Unit, and then plug the converter into a wall outlet. Positioning the Micropipette Holder Position the Pipette Holder on the microscope stage as if it were a slide. Orient the Lucite block of the pipette holder as shown in Figure 8. -
Page 15: Basic Operations For Using The Mf200
Horizontal Adjustment, position the end loop of the filament to the far right side of the visual field. Power up the MF200 unit. To do this, connect the AC/DC converter to the power input jack on the Microforge Control Unit and the wall socket. A light in the POWER switch indicates that the unit is powered up. - Page 16 NOTE: The underside of the Microforge Control Unit and the AC/DC converter become warm to the touch during use. This is normal and no action is required. Position the heating filament and the pipette: With the Power on, depress the Polish button several times at various heat settings to see the expansion of the filament loop and determine approximately where the pipette should be positioned in relation to it.
- Page 17 MF200 Table 2: Filament Power Switch Filament LARGE (or LOW) Power Position SMALL (or HIGH) Power Posi- tion When polishing large pipette tips, Will rapidly decrease filament the H2 filament works best. In life as the Heat dial approach- most cases LOW power will per- es 100.
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Page 18: Applications
Applications Choice of filaments, power and heat settings for each application vary with the use. If the desired result is achieved, the choice of parameters is acceptable. Always use the least amount of heat possible in order to prolong the life of the filaments. The distance that should be maintained between the filament and the pipette tip during microforging varies depending on the tip bore, filament, power, heat settings and application. -
Page 19: Fire Polishing A Pipette Tip
MF200 Step 2: Fire Polish the Single-Channel and Whole Cell Patch Clamp Pipette Choose and install the desired filament. (See “Table 2: Filament Power Switch” on page 13.) Turn on the Control Unit power. Hold down the Polish button and adjust the Heat Dial from low to high while observing the expansion of the filament under the microscope. -
Page 20: Tip Size Reduction
Tip Size Reduction Tip size reduction creates a holding pipette by rounding the tip ends and reducing the length of the pipette tip (Fig. 11). Fig. 11—(Right) Tip size reduction Choose and install the desired filament. (See “Table 2: Filament Power Switch” on page 13.) Heat off Turn on the Control Unit power. -
Page 21: Tip Reduction Of Large Bore Pipettes
Frequently, a beveled large bore pipette is not sharp enough to penetrate a cell without causing damage to the surrounding area. With the MF200 and the H2 heating filament, a sharp point can be formed on a beveled tip to assist in the penetration of the cell with minimal damage, using a two step process. - Page 22 Adjust the microscope stage until the pipette is in position to allow the tip to touch the filament during expansion. Set the dial so the filament starts to glow red. Press Polish and coat the center of the filament with glass until a bead about twice the diameter of the filament is formed.
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Page 23: Instrument Maintenance
Store them in their original container when not in use. In general, it is advisable to keep the MF200 in an area with minimal dust and particulates as would be appropriate for any microscope or similar apparatus. -
Page 24: Specifications
SPECIFICATIONS AC POWER MODULE 100-240 VAC 50/60Hz H2, H3, H4 FILAMENTS (3) FILAMENT “ON” CONTROL Push button or Optional Foot Switch FILAMENT ADJUSTMENT ASSEMBLY Mounts on 40× and 25× Long-Working Distance Objectives OBJECTIVE 40× Long-Working Distance (3 mm) EYEPIECE 10× (pair) RETICLE (10×... -
Page 25: Index
MF200 INDEX accessories 19 large bore pipettes 17 air supply 14 LWD 6 applications 14 maintenance 19 base plate 9 micropipette holder burn out 12 positioning 10 changing filaments 12 patch clamp pipette 14 control unit 6 pipette holder 10 platinum/iridium 6 Filament Adjustment Assembly 3, 5, 6... -
Page 26: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 27: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 28 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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