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PHOTOMICROGRAPHY
(The
Biological
Microscope
LABOPHOT
IS
designed mainly for observation.)
1. Combination of CF Objectives and
CF
PL
Projection lens
The combined use of the CF objectives and CF
PL Projection lens is essential.
For
the same total
magnification,
select a
combination
of the highest possible objective
power
and
lowest
possible projection
lens
power to achieve the utmost image definition
and contrast.
2. Checking the Illumination
Uneveness in the illumination
will
show up
more conspicuously in photomicrography
than
in observation. Consequently, before taking a
photograph, recheck the correct adjustment of
the condenser.
3. Selection of Voltage and Filter
1) When using a daylight type color fil m
Set the brightness control dial to 5.5 and use
the NCB 10 fi Iter
*-'
Adjustment
of the image brightness should be
made by means of the NO filters.
2) When using a monochrome
film
Remove the NCB 10 fi Iter. Contrast fi Iters such
as X-1 green are usable.
*-'
The NCB 10 filter is most suitable for a
standard film. Depending upon the make
of the film different color renditions may
result. It is recommended that in addition
to the NCB 10 filter a color compensation
filter
(CC filter),
available from the film
manufacturer, be used.
4. Shutter Speed
Desirable shutter speeds for least vibration are
1/4~
1/15sec.
Adjustment
of the image brightness for color
photomicrography
should be made by means of
the NO filters.
Some specimens require, on
account of their insufficient
brightness, longer
exposure times, and consequently
poor color
reproducibility
owing to the "Reciprocity
Law
16
Failure"
of film may result. So, when taking
picture of such specimens, it is recommended
to use the Nikon Biological microscope OPTI-
PHOTo
5. Manipulation of Field and Aperture
Diaphragms
In photomicrography,
the adjustment
of the
field diaphragm is important for the purpose of
limiting extraneous light which causes flare in
the microscope
image. Stop down the dia-
phragm so as to get an illuminated area slightly
larger than that of the picture field. By adjust-
ing the aperture diaphragm, a change of depth
of focus, contrast and resolution
of image is
attainable. Select a size suited to the purpose.
Generally speaking, the aperture diaphragm, is
properly stopped down to 70 ~ 80% of the
aperture of the objective being used.
6. With Regard to Condensers
For
photomicrography,
it
is generally
rec-
ommended
to use the Swing-out Achromat
condenser. When using 2X objective, however,
preferab Iy remove the condenser.
7. Focusing
Focusing is to be accomplished by means of the
ocular finder on the photomicrographic
attach-
ment, or binocular observation tube with mask
eyepiece on the trinocular eyepiece tube.
Table 3.
Focusing
Type of
Focusing with
Focusing with
eyepiece
10x or 4 x or
tube
higher objective
lower objective
Use
Use
Focusing
"F" tube
Ocular
Ocular
+
magnifier
finder
fi nder
Use
"T"
observation
Use
tube
or
observation
Focusing
or
+
magnifier
"UW" tube
tube
Ocular
finder
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