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2.
When a full field of view is observed through both tubes, and images blend into one, interpupillary
distance is corrected for your eyes. Check the interpupillary scale and note index reading for future
reference, in case other users will be changing this adjustment from time to time.
3.
Adjust the diopter scales, located on each eyepiece tube, to the same numerical value as indicated on
the interpupillary scale. This must be done in order to maintain parfocality of objective lenses. If
interpupillary distance is changed, adjust eyepiece diopters accordingly.
C.
Focusing the microscope.
1.
Position the 4x objective lens into the optical path, making sure that lens is properly indexed in its click-
stop position.
2.
Place standard specimen slide (cover slip up) on top of stage surface.
a.
Swing moveable finger on slide holder outward. Place specimen slide against fixed side of slide
holder. Slowly release moveable finger until it makes contact with specimen slide.
3.
Rotate coarse focusing controls until specimen comes into focus.
4.
Adjust fine focus controls until specimen is in sharp focus.
5.
Adjust diopter for difference in eyesight.
a.
Using right eye, peer into the right eyepiece tube. Adjust sharpness of image by utilizing fine focus
controls.
b.
Using left eye, peer into the left eyepiece tube. Adjust sharpness of image by turning diopter
adjustment located on left eyepiece tube.
6.
Adjusting the aperture (opening) of iris diaphragm.
Iris diaphragm should not be used to control the brightness of illumination, use light intensity control
knob to adjust light level. Iris diaphragms are designed to help achieve high resolution of specimen and
provide contrast in the image. Smaller apertures will deliver higher contrast to image. However, closing
aperture too much will reduce resolution. Experimentation is the best method of determining the correct
opening of diaphragm. Some suggested openings for iris diaphragm are:
OBJECTIVE
4x
10x
40x
100x
7.
Changing magnification.
a.
Rotate revolving nosepiece to position 10x objective into optical path.
b.
This microscope has been parfocalized, which allows changes from one objective to another while
requiring only a slight adjustment of the fine focus controls.
c.
When changing to the 40x and 100x objective lens, care must be exercised in order to prevent
damaging the front lens element and specimen slide.
d.
In order to obtain maximum resolution of the 100x oil immersion lens, it is necessary to apply
immersion oil between the coverglass of slide and front lens of the objective.
1)
Use of a very small amount of immersion oil is required.
DIAPHRAGM OPENING
From fully closed to 1/8 open
1/8 to 1/4 open
1/4 to 1/2 open
1/2 to 3/4 open
6
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