Sterilization By Boiling In Water Under Pressure; Other Uses Of The Autoclave; After Sterilizing Solids - Sanoclav KL Operating Instructions Manual

2.6.3 Sterilization by boiling in water under
pressure
All steps and safety measures described in Section 2.6.1 must
be observed, together with the following additional require-
ments:
1. Fill the pressure vessel with tap water until the product that
is to be sterilized is adequately covered.
CAUTION: Never fill more than ¾ of the hight of pressure
chamber.
2. To avoid breakage, glass parts should be placed in the wa-
ter while it is still cold.

2.6.4 Other uses of the autoclave

1. The temperature must be below 100°C, and the valves must
remain open, when the autoclave is used for the cultivation
of nutrient media or as an incubator. The temperature must
be controlled by adjusting the heating source accordingly.
The autoclaves are not permitted to cultivate nutrient media,
which are used for In-vitro-diagnostics.
2. With oil-bath sterilization it is necessary to use appropriate
containers to hold the sterilization oil and the instruments
that are to be sterilized.
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WARNING: After sterilizing:
1. Never open the lid while the autoclave is under
pressure. The hot steam can cause serious inju-
ries to the hands and face.
2. For taking out the still hot sterilization products
always use gloves or a suitable pliers.
Indication: If there should be formed a vacuum during cooling
down, it can be removed by lifting whistle valve
cone. For autoclaves with MCS-controller the ex-
haust valve has to be opened.
A:

After sterilizing solids:

1. Ensure that emitting steam is not directed at a person when
the steam pressure is vented.
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