Locki~ Of The Pre-Focusing Lever; Use Of Immersion Objectives - Olympus CHS Instructions Manual

Biological microscopes
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z
o
I-
~
0::
UJ
(/)
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Fig. 26
II
locking of the pre-focusing lever
This lever
(I)
is jJrovided to prevent possible conlact between speci,
men arid objeclive as well as to simplifv coarse locusing The
le~'€'r
is
locked in the direction of the arrow in Fig. 26, after coarse focus
has
been
cC'o plished,
Thi~
l<.
convenient for liquid
apol
cation or
change of specirnens, roo. since
it'
prevenl5 further upward lravel 01
,he
stage bV means oj the coarsl3 adjUst ent knobs, and provides a
I'rnitlng stop if the
St;:ly8
is lowered and then raised again. The pTe-
focusing lever does not restrict fin.e foCUsing.
*
Unlock this lever when not in use.
If)
Use of immersion objectives
1) To utilize the full numerical aperture of an irnmersion objective [wilh
inscription "oil"), the
ob~ec;tive
alld spEo:cim n i:!re
Imr'llSHed
in an im-
mersion oil in a lollowing procedure:
2) Focus on the specimen with a low power objective.
3)
Put a drop of immersion oil on the sJ:ecimen slide and the front lens
of the immersion objective.
4} Turn the nosepiece
to
bring the immersion objeclive inlO the light path,
and focus 'I,-,ith the Hne adjuslment knobs.
*
Use of the pre·focusing lever facilitates steps 2) through 4) above.
*
Care should be taken to prevent oil bubbles from forming in the oil
film; jf any. re-apply immersion oil, since these bubbles greatly
deteriorate the lens performance.
*
Be careful not to stain other objectives with immersion 'oil, and
after use, carefully wipe off the immersion oil on the objective, etc.
completely.

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