Optical Data - Olympus CHS Instructions Manual

Biological microscopes
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7
OPTICAL UATA
:
D Achromat
I
I
I
Objective
Type
o
Achromat
Magnifiction
4X
JOX
40X
100X'
N.A.
0.10
0.25
0.65
1.30
1,11/.0. (mm)
18.23
7.18
0.63
0.20
- -
Focal length (mm)
30.03
1690
4.58
166
--
Resolving pO'.ver
(p) • •
3,4
1.3
0.52
0.26
Eyepiece
Remarks
Spring-loaded
-
Total magndicalion
40X
WOX
400X
1000X
CWHK10X
Focal depth
(,u)
172.5
27.60
3.03
0.66
(Field number
18)
-
Field of view (rnm)
4.5
1.8
0.45
0.18
E D Achromat
Objective
Type
ED Achromat
Magnification
4><
lOX
40X
100X'
N.A.
0.10
0.25
0.65
1.25
W.o. (mm)
29.00
6.30
0.53
0.20
Focal length (mm)
31.05
16.45
4.59
1.90
Rt"$Olving -power
(P)'.
3.4
1.3
0.52
0.26
Eyepiece
Remarks
Spr; ng-loaded
Total magnification
40X
100X
400X
l00<..,X
CWHK10X
Focal depth
(,u)
172.5
27.60
3.03
0.67
(Field number 18)
Field of view (mm)
4.5
1.8
0.45
0.18
"Immersion obiectives
"The resolving power is obtained v-lith L1le fully opened aperture diaphragm.
Glossary
Working distance:
Numerical aperture:
The distance from the specimen or cover glass to the nearest point of the objec-
tive.
The N.A. represents a performance number which could be compared
to
the
relative aperture (f-number) of a camera lens. The quantity of light which the
objective receives from the object increases with the square of the performance
number.
Field number:
Focal depth:
Resolving power:
The resolving power of a lens is measured by its ability to separate tvvo points.
The distance between the upper and lower limits of sharpness in the image
formed by an optical system. As you stop down the aperture iris diaphragm,
the focal depth becomes deeper. The larger the N.A. of the objective the
shallower the focal depth.
A number that represents the diameter in mm of the image of the field dia-
phragm that is formed by lens in fron t of it.
Field-of-vi8'N diameter: The actual size of the field of view in mm.
II
o
"'0
-l
()
»
r
o
~
»
Total magnifiC<ltion.
Equals the objective magnification multiplied by the eyepiece magnification.
- - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 1 1

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