Nikon N-STORM Operation Manual page 35

7 Check the minimum intensity of bright points
(1) Click [Use Peak Statistics].
(2) Select the darkest bright point of all those to be
identified as molecules, and move the mouse
pointer onto its center. Read and write down the
value of [Peak Height]. (This value is used as the
minimum intensity for the identification of bright
points in the next step.)
8 Configure the settings for the identification of bright
points.
Click [Identification Settings].
In the dialog box, configure the settings as follows.
[Minimum Height]:
Specify the minimum intensity, checked in (2) of
the previous step.
[Maximum Height]:
[CCD Baseline]:
Select the [3D] checkbox.
Click [>>] to configure the following detailed settings.
[Minimum Width (nm)]: 200
[Maximum Width (nm)]: 700
[Initial Fit Width (nm)]:
[Max Axial Ratio]:
[Max Displacement (pix)]: 0 (This allows bright points
recorded in multiple frames to be analyzed as
separate molecules, increasing the accuracy of
calibration.)
Click [OK].
Restore the Max Displacement setting
When performing analysis except for calibration, reset
[Max Displacement (pix)] to the default value of 1.
Chapter 2
STORM Microscopy Operation
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7-(1)
7-(2)