N-Storm Analysis - Nikon N-STORM Operation Manual

2.3

N-STORM Analysis

Analyze an acquired dataset, perform drift correction, and identify the positions of the fluorescent probe molecules.
1 Click [Analysis GUI] to display the analysis tool.
2 Click [File Open].
A dialog box for selecting a STORM image is displayed.
3 Select the image file (ND2 file) acquired for STORM
analysis and then click [Open].
Saving open data
In the N-STORM analysis window, it is not possible to
open two or more ND2 files at once. For this reason, if a
new file is opened, the currently open file is closed. If, at
this time, the file contains any information that is not
saved, a dialog box is displayed.
- If the currently open molecule list (STORM image) has
not yet been saved, the dialog box shown on the right
is displayed. To save the molecule list, select [Yes]. If
[No] is selected, the latest molecule list will be
discarded.
- If the STORM parameter(s) in the currently open ND2
file have not yet been saved, the dialog box shown on
the right is displayed. To save the parameter(s) to the
ND2 file, select [Yes]. If [No] is selected, any changes
made to the parameter(s) will be discarded.
Results of past analysis
If STORM analysis has already been performed on the
selected ND2 file such that a corresponding molecule list
file exists, a dialog like that shown on the right is
displayed. To perform a new STORM analysis, select
[No]. (If [Yes] is selected, the saved result of the previous
analysis is read.)
Chapter 2
STORM Microscopy Operation
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