Related Manuals for Bico CYTENA C.WASH PLUS
Summary of Contents for Bico CYTENA C.WASH PLUS
- Page 1 C.WASH PLUS Practical guide (original version) Supporting Software Versions Firmware 0.1.130 and Ui App 1.0.13 or higher...
- Page 2 C.WASH PLUS | practical guide edition English 08/2024 Documentation © 2023/2024 cytena GmbH, Freiburg, Germany All rights reserved. Without the written consent of the publisher this manual or any part of it may not be in any form reproduced or distributed using electronic, mechanical or chemical processes. It is possible that the present manual still contains typographical or printing errors.
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Page 3: Table Of Contents
C.WASH PLUS | practical guide Content About this guide ........................4 General information ........................4 Setup the instrument ...................... 6 Starting the software ....................10 Getting started ......................11 Setup balance and working plate (format) ..............11 Dispensing system setup ....................12 3.2.1 Priming ........................ -
Page 4: About This Guide
C.WASH PLUS | practical guide About this guide Before activating the C.WASH PLUS device, carefully review the instructions provided in this guide and ensure to keep this document in a secure place. This comprehensive guide provides essential information about the device, its software, and its usability to ensure a smooth start for users. - Page 5 C.WASH PLUS | practical guide P a g e...
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Page 6: Setup The Instrument
C.WASH PLUS | practical guide 1 Setup the instrument 1. Unpack the instrument and tablet, then place them on a suitable bench surface. 2. Connect the power cables of both the instrument and tablet, ensuring they are securely plugged into a power outlet. 3. - Page 7 C.WASH PLUS | practical guide HINT: For dispensing valuable liquids, the dedicated inputs should be your preferred choice. By default, Input 8 is connected to the back dispense head, which features an 8- channel dispenser tailored for 96 well-plate formats. Figure 2: Representation of default dispenser configuration order: back 8 needle dispenser connects to output channel 8, middle 16 needle dispenser connects to output channel 1-6, front dispenser place holder not illustrated 5.
- Page 8 C.WASH PLUS | practical guide After assembly and tightening the screw caps to secure the tubes in place. HINT: If the tubes cannot be inserted through the screw caps, remove the caps before fitting the tubes as indicated in above figure. Repeat the procedure for all four screw caps and tubing elements or as many bottles as required.
- Page 9 C.WASH PLUS | practical guide 7. Wait for approximately 1-2 minutes, which is the required startup time for the device. During this period, the device will initiate the initialization process automatically. This process concludes when the carrier for the working plate is presented. At this point, the device is primed for operation via the C.WASH PLUS software.
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Page 10: Starting The Software
C.WASH PLUS | practical guide 2 Starting the software With the C.WASH PLUS fully configured and all solution bottles connected, it's time to delve into the software interface. The C.WASH PLUS GUI (Graphical User Interface) serves as the central interface on the PC, allowing users to operate the device in both Quick Access Mode and Custom Mode. -
Page 11: Getting Started
C.WASH PLUS | practical guide o Preinstalled Common Functions/Wizards: Offers quick initiation of commonly used functions such as "Liquid Removal," "Wash Plate," and "Dispense," along with quick access to custom protocols. • Protocols: This screen includes an overview of custom protocols, offering options to create, edit, delete, pin, and execute custom protocols. -
Page 12: Dispensing System Setup
C.WASH PLUS | practical guide Figure 8: Plate setup wizard Dispensing system setup While tubing connections were established during the general setup, it's crucial to confirm that the correct liquid reservoir is connected to the desired input. To prepare for printing with any of the dispensing units of the C.WASH PLUS, ensure that all tubes are primed with the respective liquid, at least up to the housing level. -
Page 13: Additional Precision System Functions
C.WASH PLUS | practical guide Hint: Ensure that the external tubes are fully primed with all liquids used in a protocol before initiating the protocol execution. Internal dead volume priming for the multiplexed and precision dispenser will be conducted automatically as part of your protocols whenever required. - Page 14 C.WASH PLUS | practical guide CAUTION: Ensure that you do not forget to clean your system as often as necessary to prevent issues such as needle clogging. Refer to section 13 for detailed instructions on cleaning procedures. Figure 10: Dispensing Wizard 1.
- Page 15 C.WASH PLUS | practical guide HINT: Different applications required different spinning intensities. The higher the spinning intensity the lower the residual volume but the greater forces act on your sample! 3. Washing a plate will incorporate both the liquid removal and dispensing steps, conveniently set up through the Wash Plate option.
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Page 16: Saving And Customizing Protocols
C.WASH PLUS | practical guide Figure 12: Wash Plate Wizard Protocol Generation Process Saving and Customizing protocols When saving a protocol, it can be found in the protocol tab on top right next to the “home” button. The Protocols Screen contains a list of all saved protocols. From here, protocols can be created, edited, deleted, and executed by clicking the three dots next to “run”. -
Page 17: Dispense Position And Volume Calibration
C.WASH PLUS | practical guide Figure 13: Protocols and editor screen Through the visual code editor, users can create C.WASH PLUS protocols by dragging and dropping the blocks into the sequence editor. Actions comprise predefined sequences of commands, where users only need to insert values for different parameters. Utilizing single commands to craft custom protocols can heighten complexity but also offers greater flexibility, making it ideal for more experienced users. - Page 18 C.WASH PLUS | practical guide 3. The plate can be moved in increments defined by the user by typing in the number of millimetres and pressing the arrows to adjust the plate position either inwards or outwards from the rotor chamber, thereby adjusting its position relative to the dispenser. Hint: For more gentle dispensing, it is recommended to adjust the water jet to dispense against the well wall as illustrated in...
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Page 19: Cleaning
C.WASH PLUS | practical guide 4 Cleaning Please refer to section 3 of the user manual for detailed information about safety. Please follow the cleaning instructions carefully. CAUTION: Use only specified cleaning agents and follow the instructions in this manual. The use of unspecified cleaning agents and cleaning procedures can lead to damage to the device or its failure. -
Page 20: Cleaning Of Rotor Chamber And Tubes
C.WASH PLUS | practical guide Cleaning of rotor chamber and tubes Follow the instructions on the screen and run cleaning programs on a regular basis. To do so, it is required to dilute the 10x cleaning concentration “Daily Clean”. Cleaning the housing CAUTION: Malfunction of the electronics. -
Page 21: Appendix A: Protocol Editor Example Washing A 96 Plate
C.WASH PLUS | practical guide Appendix A: Protocol Editor Example Washing a 96 plate Description of the protocol: 0. Starting point: Filled 96-well plate on plate carrier in outside position; band drive coupled with carrier 1. For liquid removal the plate moves into rotor and door closes. - Page 22 C.WASH PLUS | practical guide parameters while plate moves outside Plate moves to most outward position 22 | P a g e...
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