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Bico CYTENA C.WASH PLUS User Manual

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C.WASH Plus
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Summary of Contents for Bico CYTENA C.WASH PLUS

  • Page 1 C.WASH Plus User Manual (original version)
  • Page 2 C.WASH PLUS | User Manual edition English 08/2024 Documentation © 2020/2022 Cytena GmbH, Freiburg, Germany All rights reserved. Without the written consent of the publisher this manual or any part of it may not be in any form reproduced or distributed using electronic, mechanical or chemical processes. It is possible that the present manual still contains typographical or printing errors.

  • Page 3: Table Of Contents

    C.WASH PLUS | user manual Content Compliance Information ....................5 Manufacturer ........................5 Model reference ....................... 5 Serial number reference ....................6 CE declaration of conformity .................... 6 UKCA declaration of conformity ..................6 Applied standards ......................7 About this manual ......................7 Risk and safety information ....................
  • Page 4 C.WASH PLUS | User Manual C.WASH PLUS control PC requirements ................. 13 Materials & compatibilities .................... 13 10 Scope of delivery ......................14 11 Hardware overview ...................... 16 12 Installation requirements ..................... 19 12.1 Transport and lifting ...................... 19 12.2 Space requirements .......................

  • Page 5: Compliance Information

    C.WASH PLUS | user manual 1 Compliance Information Manufacturer Cytena GmbH Zollhallenstraße 5 D-79106 Freiburg GERMANY Reg. No.: HRB 711600 Model reference You have received the following model with the following properties. Available product models model picture C.WASH Plus Plate washer, designed for automated, rapid, and highly reproducible washing of entire microtiter plates.

  • Page 6: Serial Number Reference

    C.WASH PLUS | User Manual Serial number reference The type label can be found on the backside of each device and looks as follows: CE declaration of conformity As of the date of manufacture, this product has been tested and found to comply with the specifications for CE marking.

  • Page 7: Applied Standards

    C.WASH PLUS | user manual Applied standards Testing DIN EN 55011 :2016 + A1:2017 + A11:2020 DIN EN 61000-6-2 :2005 DIN EN 61010-1 :2010 IEC 61010-1:2010//AMD1 :2016 DIN EN 61000-3-2:2014 DIN EN 61000-3-3:2013 DIN EN IEC 63000:2018 Risk analysis EN ISO 12100 :2010 ISO/TR 14121-2 :2013 2 About this manual Before putting the C.WASH PLUS device into operation, please read the instructions given within...

  • Page 8: Risk And Safety Information

    C.WASH PLUS | User Manual The C.WASH PLUS device must only be operated by trained personnel. Cytena GmbH assumes no liability for damages that incurred due to improper handling of the device and the device components. For details, please refer to the terms and conditions (https://www.cytena.com/terms- conditions/).

  • Page 9: User Groups

    C.WASH PLUS | user manual Protective gloves When handling biological samples and using this product, it is recommended to wear appropriate gloves. Please make yourself familiar with your local, laboratory safety policies and wear the respective protective equipment. Contact your local safety officer for further information. 4 User groups Intended users This manual and the actions described within are meant to be performed by trained users.

  • Page 10: Ventilation And Air Flow

    C.WASH PLUS | User Manual proliferate within the device, when not being cleaned properly. We recommend the use of our cleaning solution at the end of the day to prevent contamination and to keep the instrument components intact. Please be aware that other solutions may be corrosive for the internal parts. Ventilation and air flow The device requires sufficient amount of surrounding air to cool its electrical components via the internal fans.

  • Page 11: Interlocks

    C.WASH PLUS | user manual Interlocks Do not defeat interlocks at any time. Do not modify or bypass interlocks. In any such cases, the operator can/will get injured severely. Only authorized and trained personnel is allowed to defeat interlocks for maintenance and service purposes. Embedded control electronics Do not connect the embedded micro-controller to a computer and/or manipulate the firmware of the micro-controller in any way.

  • Page 12: Technical Information

    C.WASH PLUS | User Manual UKCA mark NRTL mark The label (B) is the safety warning label which describes the requirements for the only personnel allowed to open the device and other particularities. The label (C) is the fuse indication label and is positioned at the power module. 9 Technical information General characteristics Dimensions...

  • Page 13: Basic Configuration

    C.WASH PLUS | user manual Internal dead volume waste 17.0 [ml] Liquid waste disposal Precision Dispense System Liquid Inputs Dispenser Needle Internal dead volume [ml] Centrifuge system 15 @ 3500 [g] @ rpm 20 @ 2000 Maximum imbalance 35 @ 1500 70 @ 1000 Centrifuge RPM 5 –...

  • Page 14: Scope Of Delivery

    C.WASH PLUS | User Manual Material compatibility matrix Legend Compatible Conditionally compatible Not compatible Not tested Matrix Stainless steel Aluminum Silicone Water Ethanol* Isopropanol* Acetone* UV (CWL 254 nm) Steam autoclave (121°C, vacuum, dry) Vaporized hydrogen peroxide (VHP gas) Bleach solutions *Tank capacity of flammable liquids must not exceed 200 ml.
  • Page 15 C.WASH PLUS | user manual Optional components Additional dispensers There are alternative carriers for standard well plates with a height of • 8 mm (CY.C2.ASS.102) suitable for some 1536 plates • 10.4 mm (CY.C2.ASS.103 ) suitable for some 1536 plates •...

  • Page 16: Hardware Overview

    C.WASH PLUS | User Manual 11 Hardware overview The following main components are relevant for the user. Figure 1: C.WASH Plus side view A. Tube connections B. Substrate carrier dock C. Front cover D. Housing Figure 2: Cable and tube connections 1.
  • Page 17 C.WASH PLUS | user manual 10 11 Figure 5: Tube Connection Ports Waste output (color code: black) 1 – 6 Inputs for dispenser 1: Ports 1-6 (color code: white) Input for dedicated dispenser 7 (color code: green) Input for dedicated dispenser 8 (color code: green) Pressure system output (color code: silver) 9 –...
  • Page 18 C.WASH PLUS | User Manual Whenever the order of dispenser placement is changed, recalibration of the dispenser position position is mandatory. Figure 6: Representation of default dispenser output and dispenser configuration order Caution: Only use the channels that have dispensers / dispense heads connected. Before running any protocol, wizard or instrument commands that operate any dispensing system (e.g.

  • Page 19: Installation Requirements

    C.WASH PLUS | user manual 12 Installation requirements Please refer to section 3 for detailed information about safety. 12.1 Transport and lifting The C.WASH PLUS device weighs 32 kg. Please always use two people to lift and carry the device. Grab the device underneath the housing, close to the feet frame.

  • Page 20: Place Of Installation

    C.WASH PLUS | User Manual 12.4 Place of installation The device must only be installed and operated indoors, ideally in a laboratory environment. It must be installed on an even, dry, flat surface that can carry the load and ensures stability. Stability in this context means that all device feet are in contact with the surface and it must be ensured that the device is firmly on the surface.

  • Page 21: Important Information Before You Start

    C.WASH PLUS | user manual Figure 8: Home screen of C.WASH Plus Software 12.6 Important information before you start Please note the following information before turning on the device for the first time! • When bringing in the device from outside, let it adapt to room temperature and conditions, before switching it on and bringing it into operation.
  • Page 22 C.WASH PLUS | User Manual When in regular use, cleaning of the device on a regular basis is recommended to avoid contamination and to ensure device integrity. Cytena GmbH recommends using the Daily Clean concentrate as daily cleaning agent after tenfold dilution. Additionally, Ethanol can be used and to spray and wipe the instrument surfaces with.

  • Page 23: Cleaning Of Rotor Chamber And Tubes

    C.WASH PLUS | user manual 13.1 Cleaning of rotor chamber and tubes Follow instructions and run cleaning programs. For more details see the practical guide. 13.2 Cleaning the housing CAUTION: Malfunction of the electronics Do not aim sprays directly at or into the instrument. •...
  • Page 24 C.WASH PLUS | User Manual The liquid input ports 1-6 and the waste may not be used with hermetically sealed bottles or liquid containers to prevent negative pressure. 3. Connect the power cable to a power outlet. 4. Connect the surface and the device by an USB-C to USB-C cable or Ethernet cable 5.

  • Page 25: Operate Device

    C.WASH PLUS | user manual CAUTION: During operation there must always be two plates + carrier in the device (working plate and balance plate). If unsure use the plate setup workflow to check and make sure there are two plate carriers + plates in the device (working and balance plate).

  • Page 26: Overview On Combination Of Plates And Carriers

    C.WASH PLUS | User Manual 14.3 Overview on Combination of Plates and Carriers The following table gives an overview of which plates and carriers to be combined with each other in the working and balance slot using C.WASH PLUS. Plate Type Carrier/Plate Combination in Working Carrier/Plate Combination in Balance Slot...

  • Page 27: Maintenance

    C.WASH PLUS | user manual 15 Maintenance 15.1 Removing the dispensers A. Flip up front cover: B. Remove the screw nut holding the dispensing heads. C. Pull the dispensers toward you from the pins. D. Unplug the Luer-Lock connection between device and dispenser you want to remove E.

  • Page 28: Customer Care Package

    C.WASH PLUS | User Manual 15.3 Customer Care Package The C.WASH PLUS devices are delivered with calibrated parameters and preinstalled software. The installation and initial start of the device can be conducted by the user. • Basic Customer Care Package (CCP) is an optional paid service that in a nutshell provides parts and labor reactive coverage much like the initial 12-month warranty.

  • Page 29: Behavior During Disturbance

    C.WASH PLUS | user manual White Moving dots Liquid Removal Blue Solid color Dispense Green Solid color (only when pump is running) Green Solid (when pump is running) Prime Green / Blue Fading Eject Plate Green / Blue Solid Ready State Solid Error State White...

  • Page 30: Error State And Corresponding Error Codes

    C.WASH PLUS | User Manual HINT: Protein-rich liquids tend to foam when being spun out. To gently remove liquid from your plate, the Decant feature can be used. Decant aims to mimics the natural swing movement with the wrist that can be used to remove liquid from the plate.

  • Page 31: General Terms And Conditions

    C.WASH PLUS | user manual Further, the error code is also mapped to the library causing the issue. This narrows down the hardware component causing the issue. Library Error code range Steprocker 100 – 199 ADC DAC library 200 - 299 IICIO Valve (standard) library 300 - 399 LED library...

  • Page 32: Legal Information

    C.WASH PLUS | User Manual 19.2 Legal information Cytena GmbH Email: info@cytena.com Zollhallenstraße 5 Website: www.cytena.com D-79106 Freiburg Tel: +49 0761 21632000 Germany District court Freiburg Managing Director / CEO: HRB 711600 Dr. Julian Riba VAT: DE295807841 19.3 Terms and conditions Please refer to the latest version of Cytena T&C under https://www.cytena.com/terms.html 32 |...

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