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Summary of Contents for revvity Bead Ruptor 19-8005
- Page 1 USER MANUAL Bead Ruptor ™ Cryo Cooling Unit Version: M Date: October 2024 Document Number: 19-8026 For research use only. Not for use in diagnostic procedures...
- Page 2 This product is warranted to be free from defects in material and workmanship for a period of ONE YEAR from the date of delivery. Revvity will repair or replace and return free of charge any part which is returned to its factory within said period, transportation prepaid by user, and which is found upon inspection to have been defective in materials or workmanship.
- Page 3 Copyright This manual is published by Revvity 935 Cobb Place Blvd, Kennesaw, GA 30144 USA. Copyright Revvity, Inc. All rights reserved, including but not limited to those rights to reproduce this publication or parts thereof. Reproduction of this publication or parts thereof, or the products it describes, by any means or in any form is expressly prohibited without the written permission of Revvity.
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Page 4: Table Of Contents
Table of Contents Table of Symbols ................................... 5 Specifications ..................................6 Site Requirements ................................. 6 System Overview .................................. 7 Installation ....................................8 General Operation ................................11 Liquid Nitrogen Pre Cooling ..............................11 Liquid Nitrogen Cooled Sample Homogenization ......................12 Dry Ice Pre Cooling ................................14 Dry Ice Cooled Sample Homogenization ......................... -
Page 5: Table Of Symbols
Table of Symbols Symbol Description Symbol Description Caution. Refer to the On (power). User documentation (IEC 60417-5007) (ISO 7000-0434B) Hazardous voltage; risk Off (power). of electric shock. (IEC 60417-5008) (IEC 60417-6042) Consult Instructions for Fuse. (IEC 60417-5016) Use. (ISO 7000-1641) Alternating current. -
Page 6: Specifications
Specifications Part Number 19-8005 Weight 15.9 lbs. (7.2 kg) Dimensions W: 9.0” (22.9 cm), D: 13.5” (34.3 cm), H: 11.5” (29.2 cm) Warranty 1 Year Cryo Chamber Volume Air Source Requirements Compressed Dry ISO8573 Class 2 or better (water < 5 ppm, total oil < 0.1 mg/m3 , vapor pressure dew point <... -
Page 7: System Overview
System Overview 1. Lid 5. Cold Air Outflow 2. Airflow Control Knob 6. Compressed Air Input 3. Insulated Cryo Hose 7. Compressed Air Hose 4. Bead Ruptor Cryo Lid 8. Thermometer The Bead Ruptor Cryo Cooling Unit consists of the following: Description Quantity Bead Ruptor... -
Page 8: Installation
Installation Locate Lid Hinges. Lift Lid and remove 4 screws using the provided Allen Key. Remove Lid Align black Cryo lid onto the Bead Ruptor Replace the four screws and tighten using the provided Allen Key. - Page 9 Position the thermometer probe under the Bead Ruptor lid as shown, using the clip to secure the thermometer probe wire under the Bead Ruptor lid. Place remaining clips as shown. Guide the thermometer probe wire through the clips. Plug the thermometer probe wire into the External Thermometer. NOTE: The Digital Thermometer has been preset to Celsius with alarms to be triggered if tempera- tures reach below -10oC or over 70oC NOTE: Compressed ISO8573 Class 2 or better air is required to use the Bead Ruptor...
- Page 10 Connect the hose from air supply to the rear of the Bead Ruptor Cryo unit. NOTE: An optional hose with a “quick connect” coupling has been included with the Bead Ruptor Cryo. An additional fitting is required to connect the hose to the air supply. Hose Barb - 3/8” or equivalent.
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Page 11: General Operation
General Operation Liquid Nitrogen Pre Cooling WARNING: Wear eye, face, hand and skin protection when working with liquid nitrogen. Operate in a well ventilated area. PRE-COOLING - follow pre-cooling procedure before homogenizing samples. NOTE: Do not load samples into the Bead Ruptor processing chamber prior to pre-cooling. Purge air lines by running dry air for 30 seconds at the MAX setting. -
Page 12: Liquid Nitrogen Cooled Sample Homogenization
Liquid Nitrogen Cooled Sample Homogenization Ensure that the knob of the Bead Ruptor Load samples into the Bead Ruptor Homogenizer. Cryo is in the off position and all hoses are Install Finger Plate connected correctly. Set the desired time, speed dwell and number of cycles on the Bead Ruptor Homogenizer. - Page 13 Turn Airflow control knob counter Allow air to flow until the external clockwise to begin cooling. thermometer reads 0oC. Once the temperature has reached 0oC, When the cycle has ended: press RUN on the Bead Ruptor to begin - Turn off compressed air supply homogenization.
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Page 14: Dry Ice Pre Cooling
Dry Ice Pre Cooling Liquid nitrogen will provide the best results. However, dry ice and ethanol may be used. WARNING: Wear eye, face, hand and skin protection when working with Dry Ice. Operate in a well ventilated area. PRE-COOLING - follow pre-cooling procedure before homogenizing samples. NOTE: Do not load samples into the Bead Ruptor processing chamber prior to pre-cooling. -
Page 15: Dry Ice Cooled Sample Homogenization
Dry Ice Cooled Sample Homogenization Ensure that the knob of the Bead Ruptor Load samples into the Bead Ruptor Homogenizer. Cryo is in the off position and all hoses are Install Finger Plate connected correctly. Set the desired time, speed dwell and number of cycles on the Bead Ruptor Homogenizer. - Page 16 Turn Airflow control knob counter Allow air to flow until the external clockwise to begin cooling. thermometer reads -5oC (23F). Once the temperature has reached 0oC, When the cycle has ended: press RUN on the Bead Ruptor to begin - Turn off compressed air supply homogenization.
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Page 17: Troubleshooting
• Contact Revvity. are defective. WARNING: Any service must be performed by a qualified service technician. This can be either an Revvity Technician or an end user resource. All parts need to be sourced from the Revvity service department. -
Page 18: Safety Notes
Safety Notes RISKS ASSOCIATED WITH LIQUID NITROGEN: It is recommended that the Bead Ruptor Cryo be operated with liquid nitrogen. Liquid nitrogen is a colorless, odorless, highly refrigerated gas (around -196oC). The main risks associated with the handling of this product are asphyxiation and burns. To protect against burns, the operator must wear equipment that protect the eyes, face and skin. -
Page 19: Appendix
Appendix This equipment is marked with the crossed-out wheeled bin symbol, to indicate that this equipment may not be disposed of as unsorted municipal waste. It’s your responsibility to correctly dispose of your equipment at life-cycle end, by handing it over to an authorized facility for separate collection and recycling of waste equipment. - Page 20 Users should exercise caution when handling materials as they may present unknown hazards. Revvity shall not be liable for any damages or losses resulting from handling or contact with the product, as Revvity cannot control actual methods, volumes, or conditions of use. Users are responsible for ensuring the product’s suitability for their specific application.
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