revvity chemagic 360 User Manual
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revvity chemagic 360 User Manual

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USER MANUAL
2024-9020-03
June 19, 2023
2024-0020
chemagic 360

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Summary of Contents for revvity chemagic 360

  • Page 1 USER MANUAL 2024-9020-03 June 19, 2023 2024-0020 chemagic 360...
  • Page 3 2024-0020 chemagic 360 Valid for instruments with software version VR6.3.0.3 Manufacturer: Wallac Oy, Mustionkatu 6, FI-20750 Turku, Finland. Tel: 358-2-2678 111. Fax: 358-2-2678 357. Website: www.revvity.com...
  • Page 5 This equipment must be installed and used in accordance with the manufacturer's recommendations. Installation and service must be performed by personnel properly trained and authorized by Revvity. Failure to follow these instructions may invalidate your warranty and/or impair the safe functioning of your equipment.

  • Page 7: Table Of Contents

    Starting the chemagic Software ..................9 Login and Rod Head Selection ..................9 The Main Program Window ................... 10 Loading/Unloading the chemagic 360 instrument with racks and/or consumables ..11 Switching off procedure ....................11 Starting a Protocol with the chemagic 12 Rod Head............. 12 Running a Protocol with the chemagic Dispenser installed ...........
  • Page 8 Table of contents Checking and priming of the dispenser manifolds ............39 Cleaning and disinfection of the chemagic 360 instrument ..........39 Cleaning of the chemagic Rod Heads ................40 Cleaning of the drop cover ....................40 Moving the instrument ....................40 Error Messages ........................

  • Page 9: General Information

    General Information General Information The chemagic 360 system can be used with broad variety of sample material and sample volumes to extract nucleic acid for research use only applications and it is not for use in diagnostic procedures. Fig. 1 Overview of the chemagic 360 instrument and its main components: 1.

  • Page 10: Principles Of Operation

    General Information Principles of Operation The instrument is used in combination with various chemagic Kits delivering high yield of pure DNA/RNA. Protocols can be added to the system by loading the appropriate protocol in to the PC controlling the instrument. The magnetic separation is achieved by metal rods, magnetized by an electromagnet.

  • Page 11: Installation And Calibration

    Installation and calibration Installation and calibration The chemagic 360 instrument installation and calibration is only to be performed by personnel trained and authorized by Revvity. Note! Instrument needs to be connected to grounded electrical outlet. Note! The instrument needs to placed on clean, dry, level, and stable surface able to bear the weight of 140kg.

  • Page 12: Specifications

    Specifications Specifications Technical specifications Width: 900mm (1500mm with display attached and door open) Depth: 820mm (1000mm with door open) Height: 900mm Weight: 140kg Input voltage: 100 - 120 / 200 - 240 V Power frequency: 50/60 Hz Power consumption: 1700 VA Noise: <...

  • Page 13: Information For Safety

    Information for safety This section describes the main safety precautions (electrical, mechanical and biological) to be taken while operating the chemagic 360 instrument as well as the main hazards involved. Please read this section very carefully before using the chemagic 360 instrument and ensure that you understand the described topics.
  • Page 14 For instructions on reagent handling, refer to the corresponding kit insert. Treat all waste as potentially infectious and dispose of it according to your local guidelines. Computer precautions Only the PC that is delivered with the chemagic 360 instrument including the original chemagic Software may...

  • Page 15: Electromagnetic Information And Precautions

    Information for safety be used to control the chemagic 360 instrument. The chemagic Software also needs to be licensed for the use in combination with a liquid handling system. Extraction protocols must not be changed by operators or anyone else who has not been trained and authorized in these matters by Revvity.
  • Page 16 Information for safety No pacemaker around the instrument. Any person with a pacemaker must keep a safety distance of at least two (2) meters from the instrument any time it is powered up. Caution: Hot surface. Do not touch. Allow for cooling before service.

  • Page 17: The Chemagic Software

    Installing the chemagic Software The chemagic 360 system is delivered with a PC on which the chemagic Software has been preinstalled. By default a folder “chemagic_360” exists in the root directory of the operating system. In this folder a file “ChemagicModule_360.exe” must be present. This is the executable for the chemagic Software.

  • Page 18: The Main Program Window

    Subsequent to the selection/detection of the chemagic Rod Head the Main Program Window will open (Fig. 4) and both axes of the chemagic 360 instrument will move into their reference positions. If this process finishes without any complications, the red lights in the “Instrument Status”...

  • Page 19: Loading/Unloading The Chemagic 360 Instrument With Racks And/Or Consumables

    During external steps the chemagic 360 instrument will pause the running protocol e.g. allowing you to access the rack carrier to add a buffer, take out a rack for a heating step etc.

  • Page 20: Starting A Protocol With The Chemagic 12 Rod Head

    Program Window (Fig. 4B) and click the green [Insert IDs] button. Depending individual configuration of your chemagic 360 system, you will have different setup Fig. 6 Instructions for connecting the buffers steps to follow: Running a Protocol with the chemagic Dispenser installed Note: If you do not have the chemagic Dispenser installed, please refer to section “Running a Protocol without the chemagic Dispenser installed”.

  • Page 21: Running A Protocol Without The Chemagic Dispenser Installed

    Starting a Protocol with the chemagic 12 Rod Head If you have less than 12 samples, you can select the exact number of samples from the drop-down menu (Fig. 7B). Please keep in mind that due to its design the chemagic Dispenser always fills entire rows i.e.

  • Page 22: Reading Ids From A File

    Starting a Protocol with the chemagic 12 Rod Head The process of sample registration is started subsequently to entering the lot number of the kit to be used (Fig. 7C). A new window which is separated into 4 different areas will open (Fig.

  • Page 23: Entering Elution Ids

    Starting a Protocol with the chemagic 12 Rod Head format and the Sample IDs have to be entered one per row. To read the IDs from a file click the [Read IDs from File] button (Fig. 8B). A dialogue window will open allowing you to select the file you have prepared earlier.

  • Page 24: Assigning Ids To The Tube Racks

    (Fig. 12B). To start the isolation process, click the [Start] button and the chemagic 360 instrument will immediately start working. During the run the Main Program Window will be active (Fig.

  • Page 25: Fig. 13 The Main Program Window During An Isolation Procedure

    Starting a Protocol with the chemagic 12 Rod Head Fig. 13 The Main Program Window during an Isolation Procedure A running process can be aborted anytime by clicking the [Stop] button. Opening one of the doors during a running process will be indicated by turning the ‘Door closed’ light to red.

  • Page 26: Fig. 15 Final Message On Completion Of A Run

    Starting a Protocol with the chemagic 12 Rod Head Fig. 15 Final message on completion of a run...

  • Page 27: Starting A Protocol With The Chemagic 24 Rod Head

    Main Program Window (Fig. 13A) and click the green [Insert IDs] button. Depending on the individual configuration of your chemagic 360 system, you will have different setup steps to follow: Running a Protocol with the chemagic Dispenser installed Note: If you do not have the chemagic Dispenser installed, please refer to section “Running a Protocol without the chemagic Dispenser installed”.

  • Page 28: Running A Protocol Without The Chemagic Dispenser Installed

    Starting a Protocol with the chemagic 24 Rod Head Fig. 18 Setting up the chemagic Dispenser for the chemagic 24 Rod Head If you have a number of samples that is not divisible by 6 you can select the exact number of samples from the drop-down menu (Fig.

  • Page 29: Entering Sample Ids (Chemagic 24 Rod Head)

    Starting a Protocol with the chemagic 24 Rod Head After clicking the [Insert IDs] button you can enter the lot number of the kit (Fig. 19) before you can continue with registering the IDs of your samples (see section below). Entering Sample IDs (chemagic 24 Rod Head) The chemagic Software offers a feature to register the IDs of your samples and their respective eluates which will be stored in the log files generated during the isolation...

  • Page 30: Reading Ids From A File

    Starting a Protocol with the chemagic 24 Rod Head Fig. 21 Entering the Sample IDs You can directly start entering your Sample IDs with the barcode scanner or by typing them into the provided input field (Fig. 21B). After entering the Sample ID (and clicking [ID entered] or hitting [Enter] for the manual input) the color changes from red to green and the ID appears in the adjacent text field.

  • Page 31: Assigning Ids To The Plates Or Tube Racks

    Fig. 24 Final instructions before starting a run To start the isolation process, click the [Start] button and the chemagic 360 instrument will immediately start working. During the run the Main Program Window will be active (Fig. 25) showing the progress of the running protocol – e.g. the elapsed time, the estimated remaining time and the time until the next external step has to be done.

  • Page 32: Fig. 25 The Main Program Window During An Isolation Procedure

    Starting a Protocol with the chemagic 24 Rod Head Fig. 25 The Main Program Window during an Isolation Procedure A running process can be aborted anytime by clicking the [Stop] button. Opening one of the doors during a running process will be indicated by turning the ‘Door closed’ light to red.
  • Page 33 Starting a Protocol with the chemagic 24 Rod Head Once a run is successfully completed, a status window will open (Fig. 27) and you can choose to view the log file of the run by clicking [Yes]. Click [No] to return to the Main Program Window.

  • Page 34: Starting A Protocol With The Chemagic 96 Rod Head

    Main Program Window (Fig. 4B) and the [Insert IDs] button will turn green. Depending on the individual configuration of your chemagic 360 system you will have different setup steps to follow: Running a Protocol with the chemagic Dispenser installed Note: If you do not have the chemagic Dispenser installed, please refer to the section “Running a Protocol without the chemagic Dispenser installed”.

  • Page 35: Running A Protocol Without The Chemagic Dispenser Installed

    Starting a Protocol with the chemagic 96 Rod Head to its design the chemagic Dispenser always fills entire rows i.e. manifolds of 12. Wells without samples in partially filled rows will be marked in orange to indicate that those positions will be filled with buffer. In case you do not use 96 well plates make sure to place empty tubes into those orange positions to avoid any spillage.

  • Page 36: Fig. 31 Positions Of The Samples On The Plates And Ids Of The Disposables

    Starting a Protocol with the chemagic 96 Rod Head The process of sample registration is started subsequently to entering the lot number of the kit to be used (Fig. 30B). A new window which is separated into 4 different areas will open (Fig.

  • Page 37: Reading Ids From A File

    Starting a Protocol with the chemagic 96 Rod Head Fig. 32 Entering the Sample IDs You can directly start entering your Sample IDs with the barcode scanner or by typing them into the provided input field (Fig. 32B). After entering the Sample ID (and clicking [ID entered] or hitting [Enter] for the manual input) the color changes from red to green and the ID appears in the adjacent text field.

  • Page 38: Assigning Ids To The Plates

    Fig. 35 Final instructions before starting a run To start the isolation process, click the [Start] button and the chemagic 360 instrument will immediately start working. During the run the Main Program Window will be active (Fig. 36) showing the progress of the running protocol – e.g. the elapsed time, the estimated remaining time and the time until the next external step has to be done (Fig.

  • Page 39: Fig. 36 The Main Program Window During An Isolation Run

    Starting a Protocol with the chemagic 96 Rod Head Fig. 36 The Main Program Window during an isolation run A running process can be aborted anytime by clicking the [Stop] button (Fig. 36C). Opening one of the doors during a running process will be indicated by turning the ‘Door closed’...

  • Page 40: Fig. 38 Final Message On Completion Of A Run

    Starting a Protocol with the chemagic 96 Rod Head Fig. 38 Final message on completion of a run...

  • Page 41: System Settings

    In the System Settings window you can setup the chemagic Software as well as the chemagic 360 instrument. To have access to all functions, please ensure that the chemagic Software is running without any warnings and that the chemagic 360 instrument is switched on and has successfully finished the calibration process of its axes.

  • Page 42: Edit User Settings

    System Settings Fig. 40 System Settings Menu Whenever you do not have sufficient rights to access a certain function a warning window will open, in which you can choose to change to a user account with Admin privileges – always provided that you know the corresponding login data (Fig. 41). Fig.

  • Page 43: Display Log Files

    Admin: Users belonging to the Admin group can additionally edit the Parameters and the User Settings and have access to the Jog Mode (Fig. 40G) which allows calibrating the axes and thus the setup of the chemagic 360 instrument. Admin users cannot edit protocols.

  • Page 44: Fig. 43 Displaying Log Files

    System Settings Fig. 43 Displaying Log files To view log files, open the System Settings Menu from the Main Program Window and click on the [Display Log Files] button.

  • Page 45: Service And Maintenance

    Fig. 45 Splash screen with software information Changing the chemagic Rod Heads The chemagic 360 instrument usually comes with an installed chemagic Rod Head. To remove a chemagic Rod Head from the system, open the front door and loosen the 4...

  • Page 46: Fig. 46 Installation Of The Chemagic Rod Heads

    Service and Maintenance in a straight angle without the risk of tilting (Fig. 46). Newer versions of the chemagic Rod Heads will have guiding brackets that minimize the risk of tilting. Fig. 46 Installation of the chemagic Rod Heads The chemagic Rod Heads have a panel with golden contact pins that connect to their counterparts on the z-axis flange on which the heads are installed (Fig.

  • Page 47: End-User Maintenance And Controls

    Cleaning and disinfection of the chemagic 360 instrument A complete cleaning of the chemagic 360 instrument is recommended once a week and whenever necessary. Always wear the appropriate protective equipment when cleaning and disinfecting the chemagic 360 instrument (laboratory coat, gloves, and safety glasses).

  • Page 48: Cleaning Of The Chemagic Rod Heads

    Service and Maintenance disinfection allow an incubation time of 1 hour prior to cleaning of the treated surfaces with a wipe soaked with deionized water. Subsequent to the Kohrsolin treatment removal of nucleic acids can be done with e.g. DNA-Exitus or similar formulations – followed by cleaning with deionized water (wipe).

  • Page 49: Error Messages

    Error Messages Error Messages Error messages during startup of software Error number 001: The Program is already running  The program is already running and has not been closed yet. Error number 002: Unable to open Parameter File.  The parameter file 'Parameter.msm' could not be opened (e.g. file not available in program directory).

  • Page 50: Error Messages During Reading Or Saving Protocol File

    Error Messages Error number 036: Unable to save Axis Parameter File.  The parameter file 'AxisParameter.msm' could not be saved. Error number 038: Value for Axis Parameter --- not valid or out of range.  The entered value for the axis parameter is invalid or outside of the allowed range.

  • Page 51: Error Messages While Editing Protocol File

    Error Messages Error while reading data from protocol file in line ---, the speed value exceeds setup maximum speed. Error number 046: Error in Pattern, Step No. ---. One of the Speed Parameters exceeds the max Speed Limit. One of the speeds entered at the dialog ‘Edit Protocol’ ijns step --- exceeds the setup maximum speed.

  • Page 52: Error Messages While Reading Data From Dialog Edit Fields

    Error Messages Error number 063: Error read External Process Data from Protocol.  The data for external steps from the info text area of the protocol file is invalid. Warning number 063: External Process Data could not be read from Protocol. ...

  • Page 53: Error Messages / Warning Log-Files

    Error Messages  The selected function is not allowed for the actual user level. When selecting 'Yes' the login dialog is opened to log-in with a different user name. Warning number 094: Function not allowed for actual User Level.  The selected function is not allowed for the actual user level. Error number 095: Error read User Data Settings from File.

  • Page 54: Error Messages / Warnings Drop Cover

    Error Messages Error messages / warnings Drop Cover Warning number 213: Drop Cover must be open before moving Stripper down.  The drop cover must be open before the stripper can be moved to the lower position (Jog-Modus). Warning number 214: Drop Cover must be open before moving Z-Axis down. ...
  • Page 55 Error Messages Error number 243: Axis ---: Disable axis failed with error ---.  Error disable axis ---. Error number 244: Axis ---: Change axis parameters failed with error ---.  Error while transferring axis parameter for axis --- to motor card. Error number 245: Axis ---: Homing axis failed with error ---.

  • Page 56: Error Messages Communication Io-Modules / Axes

    Error Messages Error messages communication IO-Modules / Axes Error number 300: A Communication Timeout occurred in Communication to IO Module with Address --- (Result = ---). Select another Com-Port and check Supply Voltage and Communication Line to the Modules on the RS485 IO Bus. Check also whether you attached several RS485 IO Modules with the same Communication Address at the Bus.
  • Page 57 Error Messages Internal Error number 406: Undefined Error Step detected in Production Thread.  Internal software error: should not occur under normal conditions.

  • Page 58: Troubleshooting

    Possible Reason Solution Make sure the power chord is The power chord is not The chemagic 360 instrument connected to the chemagic 360 connected to the chemagic cannot be switched on instrument and plugged into the 360 instrument power socket...

  • Page 59: Table Of Figures

    Table of figures Table of Figures FIG. 1 OVERVIEW OF THE CHEMAGIC 360 INSTRUMENT AND ITS MAIN COMPONENTS: FIG. 2 SPLASH SCREEN WITH SOFTWARE INFORMATION FIG. 3 LOGIN SCREEN AND ROD HEAD SELECTION FIG. 4 THE MAIN PROGRAM WINDOW FIG. 5 REMINDER FOR INSTALLING THE DROP COVER FIG.

  • Page 60: Weee Instructions For Revvity Products

    Customer Care USA: call 1-800-762-4000 Customer Care Rest of the World: call (+1) 203-925-4602 Products from other manufacturers may also form a part of your Revvity system. These other producers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive.

  • Page 61: Index

    Installing the chemagic Software, 9 Automated isolation procedure, 11 Instrument Status, 10 Changing the chemagic Rod Heads, 37 Intended use of the chemagic 360, 1 chemagic 12 Rod Head, starting a Protocol with the, Kit lot number, 13, 20, 27 License window, 9...

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2024-0020

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