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WPI UMP3T-1 Instruction Manual

With micro2t smartouch controller
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INSTRUCTION MANUAL
UMP3T-1
With MICRO2T SMARTouch™ Controller
www.wpiinc.com
080624
080624

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Summary of Contents for WPI UMP3T-1

  • Page 1 INSTRUCTION MANUAL UMP3T-1 With MICRO2T SMARTouch™ Controller www.wpiinc.com 080624 080624...

  • Page 3: Table Of Contents

    UMP3 with SMARTouch™ Controller CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Notes and Warnings ......................... 2 UMP3 ............................2 UMP3/MICRO2T Parts List ...................... 4 Unpacking ........................... 4 INSTRUMENT DESCRIPTION ......................5 UMP3 Description ........................5 MICRO2T Description ....................... 5 UMP3 Description ........................
  • Page 4 World Precision Instruments...

  • Page 5: About This Manual

    Fig. 1—The MICRO2T SMARTouch™ controller is a touch screen controller for use with the UMP3 and Nanoliter series pumps. The UMP3 shown here is mounted on WPI’s M3301 micromanipulator and TB-1 stand (not included). Mounted on the UMP3 is our signature NanoFil™...

  • Page 6: Introduction

    INTRODUCTION WPI’s SMARTouch™ controller may be used with the UltraMicroPump III (UMP3) syringe pump or the Nanoliter 2020 positive displacement pump. This manual will describe the use of the UMP3 and the Nanoliter 2020 pumps with the SMARTouch™ controller. Notes and Warnings CAUTION: DO NOT operate the UMP3 until you have programmed your end-stop position on the MICRO2T controller.
  • Page 7 An optional foot switch (WPI# 15867) can be plugged into an 3.5mm connector on the rear of the controller for hands free start /stop operation.

  • Page 8: Ump3/Micro2T Parts List

    The kits (UMP3-1, UMP3-2) include a controller. UMP3-1 includes one UMP3 pump, UMP3-2 includes 2. For a list of microsyringes available from WPI, see “Syringes” on page 37. Unpacking Upon receipt of this instrument, make a thorough inspection of the contents and check for possible damage.

  • Page 9: Instrument Description

    UMP3 with SMARTouch™ Controller INSTRUMENT DESCRIPTION UMP3 Description Fig. 4—System picture of the UMP3 with the controller. A typical setup includes a micromanipulator, a base stand and a syringe, which are not included. MICRO2T Description The MICRO2T is used to control one or two UMP3 syringe pump or Nanoliter 2020 positive displacement pumps.
  • Page 10 Configuration Menu Pump Information Display Pump Information Display Showing Two Pumps 2-Channel Command Selected Pump Command Buttons Configuration Menu Pump Information Display Fig. 6—The Command screen provides options for setting your inject/withdraw parameters per channel and allowing for the precise toggling of your selected pump(s). Selected Pump–Touch one of the pump channels in the Pump Information Display area to select a channel.
  • Page 11 UMP3 with SMARTouch™ Controller mode, delivery rate, selected syringe, motor drive mode, units of delivery and grouping status. (See “Setting Parameters” on page 14.) Pump Information Display–This area shows information about each pump connected to the controller. Fig. 7—The pump display shows information for each programmed pump attached to the controller.

  • Page 12: Ump3 Description

    Collar Stop. Always check the Collar Stop to verify that the syringe is held firmly. Default position is for WPI NanoFil syringe collar. If necessary, adjust the collar stop placement using the Phillips head screw. Adjustment allows for ease of removal without damage to glass syringes.

  • Page 13: Setting Up The System

    Positions for the Mounting Bar Fig. 10—Unscrew the mounting rod to reposition it. WPI’s UMP3 fits directly into most stereotaxic frames that accept standard probe or electrode holders. The UMP3 mounting bar diameter is 7.90mm (0.311 in.). For example, UMP3 fits directly into KOPF Standard 900 series frames (replacing of 1770 electrode holder).
  • Page 14 Connect the power cord to the power supply, and plug it into an electrical outlet. If the foot switch (WPI# 13142, not included) is needed, plug it into the foot switch port on the rear panel of the MICRO2T controller.

  • Page 15: Choosing A Syringe

    UMP3 with SMARTouch™ Controller Fig. 11—The UMP3 collar stop adjustment is located in the groove on top of the UMP3. With the #0 Phillips screwdriver, loosen this screw slightly (about 0.5 to 1 mm) to allow for a thicker collar. If necessary, grasp the collar stop and wiggle it backwards to move it.
  • Page 16 the minimum acceptable injectable volume from this 10µL (10,000 nL) syringe is 0.5276nL × 10 or 5.276nL. NOTE: Each type of syringe yields a different value depending on its inside diameter and the volume per step. The MICRO2T calculates the minimum volume and displays it on the Syringe Selection screen.
  • Page 17 ** The ILS005 5µL syringe must be defined as a custom syringe with a length of 28 mm. † The NL syringe type is reserved for WPI’s NANOLITER2020, a nanoliter injector for the 2-70 nL range. It comes with its own simple controller, but may also be driven by the MICRO2T. For more information, see the Nanoliter 2020 Instruction Manual.

  • Page 18: Setting Parameters

    Syringe Stroke Length The delivery of the UMP3 is based on 60 mm or 54.1 mm syringes. Please note which syringe length you are using. You may need to adjust the syringe length. Maker Syringe Stroke Length Use Type NanoFil™ 10µL, 100µL 60 mm Hamilton 1700 Series, 10µL...

  • Page 19: Accessing The Configuration Screen

    UMP3 with SMARTouch™ Controller Accessing the Configuration Screen Select the appropriate pump channel on the Command screen by tapping the desired channel on the display. Fig. 15—Channel 1 is selected, and PUMP1 is shown with a blue field behind it in the upper right corner of the display screen.
  • Page 20 running unattended. This prevents the possibility of binding the lead screw which can cause the pump to sieze. The calibration procedure involves using the MICRO2T controller to position the UMP3 into the fully withdrawn position. Press and hold the Set Stop option on your MICRO2T.
  • Page 21 UMP3 with SMARTouch™ Controller Setting Pump End of Travel Limits for the UMP3 To access the Configuration screen, select a channel by tapping on the display from the Command screen. Then, click Configure for your selected pump. See “Accessing the Configuration Screen” on page 15. Press Reset Pos to open the Syringe Stop Definition Screen.
  • Page 22 To access the Configuration screen, select a pump by tapping on the display from the Command screen. Then, click Configure for your selected pump. See “Accessing the Configuration Screen” on page 15. Tap Reset Pos to open the Syringe Stop Definition screen. Fig.
  • Page 23 UMP3 with SMARTouch™ Controller Fig. 19—This warning displays if the controller loses power while a pump is running. Press OK to continue operation. The message indicates which pump or pumps require attention. Reset the end stop and syringe limits for the displayed pump or pumps. Until the limits are reset, the message continues to display on power up.
  • Page 24 To update the volume target or the delivery rate, tap the text field. A keypad appears with 0.0 in the text field. Use the keypad to enter the desired value. Fig. 21—Use the keypad to change the Volume Target. A similar keypad appears when you press the Volume Counter or the Delivery Rate fields.
  • Page 25 UMP3 with SMARTouch™ Controller • Max Load yields a more pulsatile flow with increased delivery force. It allows the pump to deliver at the desired rate with the fewest number of microsteps per step. Smooth Delivery Max Load Smooth undesirable pressure pulses when Increased delivery force: injecting low-volume samples or for injections 15–30% increase over Smooth...

  • Page 26: Selecting System Options

    Selecting System Options The System Options screen gives you immediate access to basic parameters for your operations. To access the System Options screen: • Select System Options from the main menu, or • From the Pump Configuration screen, press Options. Fig.

  • Page 27: Setting Screen Brightness

    UMP3 with SMARTouch™ Controller Disabling Motors for Low Noise Recording For applications where a low electrical noise environment is necessary, you may turn off the drive electronics to the motors until you need to use them. When you return to any other screen, the motors automatically re-enable themselves.

  • Page 28: Defining A Custom Syringe For Use With The Ump3

    Defining a Custom Syringe for Use with the UMP3 Ten microsyringes with volumes ranging from 0.5µL to 250µL are already preset in the MICRO2T. These include syringe types 1 through 9 and NL (for the Nanoliter 2020). See “Syringe Types” on page 13. A microsyringe with a volume other than those preset may be entered as syringe type A, B or C.
  • Page 29 UMP3 with SMARTouch™ Controller Fig. 27—You can define custom syringes using the Syringe Selection screen. Select one of the three user-defined syringe types–A, B or C. Touch the syringe type you want to edit (A, B or C). This highlights the selected syringe.
  • Page 30 Use the keypad to enter a new value for the selected syringe parameter. • Press Enter to save the value and return to the previous screen. • Press Back to return to the previous screen without saving the data. If you are unsure of the length of your syringe, use the direct measurement method.

  • Page 31: Operating Instructions

    UMP3 with SMARTouch™ Controller OPERATING INSTRUCTIONS When the pump runs, an LED on the head of the UMP3 illuminates to indicate that the pump is receiving a signal from the controller. As the pump runs, the counter increments as an indication of the plunger’s motion. NOTE: Before operating an UMP3, it is important to establish the limits of travel.

  • Page 32: Sending Commands To A Pump

    Sending Commands to a Pump Fig. 31—Use the Command screen to control the pumps. • Run starts the programmed sequence for the selected pump. As soon as you press Run, the button changes to Stop on a red background and the Direction button changes to Pause.

  • Page 33: Calibrating A Syringe

    UMP3 with SMARTouch™ Controller Fig. 32—The Navigation screen lets you manually position the syringe prior to a delivery. Use this screen to inject or withdraw the pump at two different rates. (Fast and Slow). Withdraw Infuse Slow Fast The movements can be momentary or continuous. •...

  • Page 34: Sample Operational Preparations

    Volumetric Diameter Measurement Using Calibrated Scope Using a microscope and a calibrated reticle or stage micrometer, inject water into a hydrated oil droplet Using the reticle, measure the sphere. Calculate the volume of the sphere (V= [4/3] x πr ) in nanoliters using the equation: Volume = (4/3) ×...
  • Page 35 UMP3 with SMARTouch™ Controller Select Display All Channels to open the Command screen. Fig. 34—The Command screen provides real time data on the connected pumps. Select the desire pump by tapping the information display area for that pump. Then, press Configure to open the Configuration screen for the selected pump. Fig.
  • Page 36 • Remaining starts at the target volume and decrements to zero. See “Defining Pump Parameters” on page 20. 11. Use the round buttons to choose the delivery rate units, either nL/min or nL/sec. 12. Disabled pumps will not display. Grouped pumps start and stop concurrently when any pump in the group is commanded.

  • Page 37: Grouped Operations

    UMP3 with SMARTouch™ Controller 19. When changing direction, we recommend traveling a minimum of three times the minimum recommended volume shown in the syringe selection screen for the selected syringe type. This ensures that the injection will deliver the desired volume when commanded.

  • Page 38: Computer Control

    When you finish setting up the pump and syringe parameters, press Back to return to the Command screen. Fig. 38—Pump 2 is the Master pump, and Pump 1 is the slave in this example. Press Run to infuse the required volume of fluid for the injection or for multiple injections.
  • Page 39 UMP3 with SMARTouch™ Controller Command Syntax Notes Halt volume delivery (STOP) Cancels delivery in process Pause delivery Pauses delivery in process Set delivery units to nL/sec Set delivery units to nL/min Set active pump # is between 1 and 2 Set mode to non-grouped Set mode to grouped Set mode to disabled...
  • Page 40 Command Syntax Notes Beep F#### #### is length of beep in seconds/100 Response: BEEP (while beeping) OK (when finished beeping) Kill command Ends current command and cancels any pending commands Blocking start delivery Same as G command, but it stops accepting any new commands until the commanded delivery is finishd at which point it responds with OK.

  • Page 41: Maintenance

    Excessive dust can cause jams and inadvertent stops. ACCESSORIES Syringes UMP3 is designed to be used with glass syringes having barrel diameters from 5.5 to 9mm. WPI stocks the following syringes (with replaceable beveled needles): Syringes with Beveled Needles Stroke O.D.

  • Page 42: Additional Accessories

    Replacement Needles Order No. Description NF33BV/ For NanoFil™ Syringes, 33 ga BEVELED or BLUNT (40mm total fixture, NF33BL injection needle length 10mm), 137mm total length installed within syringe barrel excluding plunger NF34BV/ For NanoFil™ Syringes, 34 ga BEVELED or BLUNT (35mm total fixture, NF34BL injection needle length 5mm), 127mm total length installed within syringe barrel excluding plunger...

  • Page 43: Troubleshooting

    (a condition which can be caused by overtravel), the pump must which can be caused by overtravel), the pump must be returned to WPI for repair. be returned to WPI for repair. Selected pump is Selected pump is...
  • Page 44 If you require a large volume syringe (over 250µL), use a 250µL), use a liquid-tight plunger. liquid-tight plunger. NOTE: If you have a problem/issue that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or technicalsupport@wpiinc.com. World Precision Instruments...

  • Page 45: Specifications

    UMP3 with SMARTouch™ Controller SPECIFICATIONS This unit conforms to the following specifications: UMP3 Travel ............................62 mm Minimum Dispensing Volume ..........0.58 nL/step (syringe dependent) Linear Motion ......................6.35 µm/step Plunger Position Error ......................< 0.5% Pump Force ..........................400 g Syringe Diameters .....................5.5 to 9.0 mm Maximum Step Rate ............560 steps/sec (depending on syringe) Weight ........................325 g (11.4 oz.) Size ..................∅...
  • Page 46 Order Total Shank Bevel Tip O.D. Tip I.D. Length Length O.D. Length Material NF36BV 120 μm 35 μm 3 mm 33 mm 460 μm ≈156 µm Stainless Steel NFQ34-5 160 μm 100 55 mm 75 mm 460 μm n/a Quartz μm NF33BL Stainless...

  • Page 47: Appendix A: Using Teflon Tipped Syringes

    UMP3 with SMARTouch™ Controller APPENDIX A: USING TEFLON TIPPED SYRINGES Carefully remove the plunger and its Teflon tip by drawing it out of the syringe barrel. Before inserting the plunger tip into the syringe, pre-wet the Teflon plunger tip and the syringe body interior with water. CAUTION: Use care in inserting the plunger into the syringe, because the plunger rod may be easily bent.

  • Page 48: Declaration Of Conformity

    DECLARATION OF CONFORMITY World Precision Instruments...
  • Page 49 UMP3 with SMARTouch™ Controller World Precision Instruments...
  • Page 50 World Precision Instruments...
  • Page 51 UMP3 with SMARTouch™ Controller World Precision Instruments...

  • Page 52: Index

    INDEX delivered volume 7 manual 28 Symbols delivery rate 19 max load 20 13142 39 direction 6, 28 MICRO2T 5 65085 39 disabled 21 micrometer 30 65141 39 disable motors 23 misalignment 40 300630 39 disassemble 2 motor drive 20, 31 motors, disable 23 mounting a syringe 10 Accessory Kit 4 End Stop calibration 15...
  • Page 53 UMP3 with SMARTouch™ Controller screen brightness 23 withdraw 28 sending commands 28 serial commands 34 set syringe 16 SGE 38 SGE001RN 37 SGE0005RN 37 SGE025RN 37 SGE050RN 37 SGE050TLL 38 SGE100RN 37 SGE100TLL 38 SGE250TLL 38 solvents 2 sound 22 specifications 42 stall 41 stalled pump 16...

  • Page 54: Warranty

    WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product.
  • Page 55 UMP3 with SMARTouch™ Controller World Precision Instruments...
  • Page 56 W OR LD P RE C I SI O N I NSTR U M E N TS...

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