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Summary of Contents for WPI PicoPump PV830
- Page 1 PV830 Pneumatic PicoPump INSTRUCTION MANUAL Serial No._____________________ 042712 World Precision Instruments...
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Page 3: Table Of Contents
PV830 Pneumatic PicoPump CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 General ............................. 1 Notes and Warnings ........................1 Unpacking ............................2 INSTRUMENT DESCRIPTION ......................3 Setup ..............................5 Vacuum Input ..........................6 Pressure Input..........................6 Eject Pressure Port (front panel) ....................6 Vacuum Port ........................... - Page 4 World Precision Instruments...
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Page 5: About This Manual
PV830 Pneumatic PicoPump ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. -
Page 6: Unpacking
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 23 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. -
Page 7: Instrument Description
The Startup Kit (WPI #3316) includes: (1) 0.25” NPT Fitting for nitrogen tank regulators (2) 0.25” quick connect fit barbs (use on vacuum side, where applicable) (1) 5’... - Page 8 Fig. 3—(Left) The PV830 Startup kit includes tubing and several fittings. Fig. 4—(Right) The threaded fitting is for a nitrogen regulator. Depress the plastic collar to remove tubing. Fig. 5—The PicoPump Pressure and Vacuum ports are located on the rear panel of the PV830.
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Page 9: Setup
PV830 Pneumatic PicoPump Setup Firmly insert tubing into the quick-connect fittings on the rear of the instrument. To remove tubing, press in on the surrounding collar while pulling the tubing out. Plastic tubing can be disconnected easily, while metal tubing may require more effort. For a diagram of the setup, see Fig. -
Page 10: Vacuum Input
Connect the Vacuum Input (located at the back of the instrument) to a suitable source of vacuum, such as a vacuum pump (WPI #LM-500865) or aspirator, using 0.25˝ OD hard tubing and the soft plastic connector (supplied). Vacuum may be anywhere in the range of 0–30 in. - Page 11 When using a MPH6S handle, a firmer hold on the glass can be achieved by using two gaskets in the micropipette holder. NOTE: Capillary holders can be obtained from WPI which contain Ag/AgCl half-cells. These holders (MPH6S, MPH6R) can be easily mounted upon amplifier headstage probes so that potential and/or current, as well as pressure, can be measured or dispensed through the capillary tip.
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Page 12: Vacuum Port
(2) 5’ tubing (4) 1.0mm pipette gaskets (green) (4) 1.2mm pipette gaskets (black) (4) 1.5mm pipette gaskets (red) (4) 1.65mm pipette gaskets (white) Handle Tubing Body Gasket Tubing connected to the barb Pipette Fig. 9—The 5430-ALL (version 2) must be properly assembled. Before use, the superthane tubing must be slid through the handle and connected to the barb of the body. -
Page 13: Operating Instructions
PV830 Pneumatic PicoPump OPERATING INSTRUCTIONS Front Panel Fig. 10—PV830 Front Panel. CAUTION: The valves always pass some air. Do NOT apply too much pressure when turning the knobs. They will not reach zero while the PV830 is running. Over-tightening the knobs will damage the regulators. Pressure Adjustment–Adjust the pressure by turning the Regulator knobs located next to the pressure gauges. -
Page 14: Rear Panel
the regulating mechanism is self- venting, vacuum is automatically released when the regulator setting is decreased. Although the vacuum gauge cannot be read accurately at very low vacuum settings (below 2 in. Hg), reproducible settings may still be obtained by means of the 20-turn dial regulator knob. Vacuum–When the Vacuum switch is in the vac position, the vacuum output port is connected to the regulated vacuum line. -
Page 15: Techniques In Microinjection
PV830 Pneumatic PicoPump Techniques In Microinjection One of the more demanding tasks for which the PicoPump has been designed is the microinjection of fluid into cells. Difficulties encountered will not usually involve the mechanics of the PicoPump, but rather the manufacture of suitable micropipettes. Both care and steady hands are needed in making pipettes to take full advantage of the features of the PicoPump. -
Page 16: Micropipette Manufacture
the pipette. However, a 0.1–0.2 PSI pressure imbalance will not cause a significant problem if the tip is small enough. The gravity of the fluid and the flow resistance caused by friction from the glass wall will both help to stop the solution flow at this pressure level. - Page 17 Fig. 11—(Left) The volume of a droplet increases as the diameter increases. See “Appendix” on page 16. Fig. 12—(Right) WPI’s MicroFil is helpful in backfilling glass pipette tips. Syringe filters (available separately) help prevent clogged micropipette tips. The following table is useful for converting between different units of volume.
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Page 18: Multibarrel Microinjection
When pumping electrolytes, some indication of flow may be obtained by monitoring the resistance or the voltage of the micropipette. If, for example, the micropipette is filled with 3M KCl and the sample fluid is 0.1M KCl, a significant decrease in resistance of the micropipette occurs when pressure is applied. -
Page 19: Accessories
PV830 Pneumatic PicoPump ACCESSORIES Part Number Description 3260 Foot Switch 2932 Rack Mount Kit, 31 2-in. high (PV800 & PV820) 2933 Rack Mount Kit, 51 4-in. high (PV830) 5430-10 PicoNozzle 1 Kit (MPH6S for 1.0 mm pipette & 5-ft tubing assembly) 5430-12 PicoNozzle 1 Kit (MPH6S for 1.2 mm pipette &... -
Page 20: Troubleshooting
Turn off the Hold pressure valve before assembly and disassembly of the injection pipette. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. World Precision Instruments... -
Page 21: Specifications
PV830 Pneumatic PicoPump SPECIFICATIONS This unit conforms to the following specifications: Pressure Pressure Input (rear) ..............150 PSI (1000kPa) maximum Pressure Output ....................0–90 PSI (600kPa) Hold Pressure Output ................0.2–10 PSI (1.4 to 69kPa) Lowest Regulated Pressure ................12 in. water (3kPa) * Regulator Accuracy .................. -
Page 22: Physical Specifications
Physical Specifications Power .95-130 VAC or 190-260 VAC, switch-selectable, single-phase, 50/60 Hz, 20VA Temperature..................10ºC (50ºF) to 40ºC (104ºF) Dimensions ....3.5 x 17 x 9.5 in. (89 x 432 x 242 mm) Mountable in standard ANSI/EIA RS310C 19 in. rack. World Precision Instruments... - Page 23 PV830 Pneumatic PicoPump APPENDIX Use the chart below to guage the volume of a droplet and the table to determine the volume a micropipette can hold. 215µm 1mL = 1.24cm 10nL = 267µm 4.64mm 100µm 100µL = 5.76mm 1nL = 1.24µm 2.15mm 46µm...
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Page 24: Bibliography
BIBLIOGRAPHY R.E. McCaman, D.G. McKenna and J.K. Ono, A Pressure System for Intracellular and Extracellular Ejections of Picoliter Volumes , Brain Research 136 141-147 (1977) T.W. Stone, Microintophoresis and Pressure Ejection (1985) Y. K llstrom and S. Lindstr m, A Simple Device for Pressure Injections of Horseradish Peroxidase into Small Central Neurons , Brain Research 156 102-105 (1978) R.E. - Page 25 PV830 Pneumatic PicoPump World Precision Instruments...
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Page 26: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 27: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 28 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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