10x Genomics Chromium X Series User Manual

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User Guide | CG000396 | Rev K

Chromium X Series Instrument

with Readiness Test

For use with:

Chromium X & Accessory Kit

12-Month Warranty, PN-1000331 | 24-Month Warranty, PN-1000332

Chromium iX & Accessory Kit

12-Month Warranty, PN-1000328 | 24-Month Warranty, PN-1000329

Chromium Xo & Accessory Kit, 12-Month Warranty, PN-1000812

Chromium X Series Refurbished

PN-1000326R | PN-1000818R

10xgenomics.com

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Page 1 User Guide | CG000396 | Rev K Chromium X Series Instrument with Readiness Test For use with: Chromium X & Accessory Kit 12-Month Warranty, PN-1000331 | 24-Month Warranty, PN-1000332 Chromium iX & Accessory Kit 12-Month Warranty, PN-1000328 | 24-Month Warranty, PN-1000329 Chromium Xo &...
Page 2: Document Number
© 2024 10x Genomics, Inc. (10x Genomics). All rights reserved. Duplication and/or reproduction of all or any portion of this document without the express written consent of 10x Genomics, is strictly forbidden. Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any products described herein.
Page 3: Revision Date
Discontinued kit tables were moved to the Appendix (p. 45). • Updated screenshots to include the firmware version number in the upper left corner. • Updated the name of the instrument throughout to read “Chromium X Series instrument” for consistency. Link to TOC | 10xgenomics.com...
Page 4: Table Of Contents
CG000396 | Rev K User Guide | Chromium X Series Instrument | Table of Contents Table of Introduction Introduction Contents Product Identification Label Product Specifications Safety & Compliance Information Safety Regulatory System Components System Components Accessory Kits System Operation Step 1: Installation...
Page 5: Introduction
Introduction Introduction Product Identification Product Specifications...
Page 6 CG000396 | Rev K User Guide | Chromium X Series Instrument | Introduction The Chromium X Series is the next generation of instrumentation, Introduction purposefully designed to enable high-throughput experiments. Chromium X Series instruments include: • Advanced sensors, improved pressure control systems, and precise temperature control on the tray.
Page 7 CG000396 | Rev K User Guide | Chromium X Series Instrument | Introduction A comparison of the various capabilities of the Chromium X Series Introduction instrument firmware licenses: contd. Assay GEM-X Single Cell 3’ v2.2.0 v2.0.0 v2.0.0 Upgrade to X GEM-X Single Cell 5’...
Page 8: Product Specifications
CG000396 | Rev K User Guide | Chromium X Series Instrument | Introduction Product Identification Label Image of label for reference only Product Parameter Specifications Specifications Weight 41.4 lb (18.8 kg) Instrument Dimensions with: Tray close 12.2”[31.0 cm] × 18.6”[47.2 cm] × 10.2”[26.0 cm] Tray open 12.2”[31.0 cm] ×...
Page 9: Safety & Compliance Information
Safety & Compliance Information Safety Regulatory...
Page 10: Safety
CG000396 | Rev K User Guide | Chromium X Series Instrument | Safety & Compliance Information Before operation, ensure that all potential users have received: Safety • Instruction in general safety practices for laboratories. • Instruction in specific safety practices for the instrument.
Page 11: Regulatory
CG000396 | Rev K User Guide | Chromium X Series Instrument | Safety & Compliance Information The Chromium X Series instrument has been designed, tested, and Regulatory certified to be in compliance with the following standards: Certification Standards TUV Certification only for Chromium X Series instrument UL 61010-1:2012 and CAN/CSA C22.2 No.
Page 12: System Components
System Components System Components Accessory Kits...
Page 13 CG000396 | Rev K User Guide | Chromium X Series Instrument | System Components System Components Front Touchscreen Tray Tray Finger Access Area Indentations for chip holder feet Locater Pins Rim for chip holder placement Back USB Ports Power Supply...
Page 14: Accessory Kits
USB, 8 GB ** 3000664 * Blank gaskets have no holes. Use only with shipping/readiness test block. Not compatible with the chip holder. ** The Chromium X Series instrument is compatible with VFAT, FAT32, and exFAT formatted USBs. GEM-X Transition Kit Product...
Page 15: Additional Items
CG000396 | Rev K User Guide | Chromium X Series Instrument | Introduction Additional Items Product Part Number Shipping/Readiness Test Block* 3000652 Chip Gasket, Blank X Series** 3002180 Interchangeable with 3001663 Shipping Lock 1000657 Located inside the tray door during transportation to secure and stabilize; removed during installation and stored apart from the instrument.
Page 16: System Operation
System Operation Step 1: Installation Step 2: First-Time Use Step 3: Readiness Test Main One-Touch Operation...
Page 17: Step 1: Installation
Warning: Avoid using the Chromium X Series instrument in a manner not specified by 10x Genomics. The instrument has been designed to protect the user. If used improperly, the intended user protections can be impaired. General Power Safety Grounding is required to prevent electric shock.
Page 18 Two-person lift required. Use the tote bag included in the packaging to lift the instrument itself (41.4 lb [18.8 kg]). The Chromium X Series Accessory Kit (PN-1000821) shown in the diagram is comprised of the components formerly listed in both the Chromium iX Accessory Kit (PN-1000323) and the Chromium X Upgrade Kit (PN-1000327).
Page 19 Within the Accessory Kit box, locate the universal power cord and plug adaptors. Attach the correct country adaptor to the universal adaptor. d. Plug the Chromium X Series instrument into a properly grounded outlet of proper voltage. DO NOT power on yet.
Page 20: Step 2: First-Time Use
CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation Step 2: The instrument will walk through a first-time setup protocol. Setup requires the following: First-Time Use • 10x Genomics Account Onscreen instruction will guide account setup. Alternatively, visit cloud.
Page 21 CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation d. Register the instrument. Step 2: First-Time Use Navigate to register.10xgenomics.com using the onscreen QR code on a contd. computer or phone with internet access. Create or log in to a 10x Genomics account.
Page 22: Step 3: Readiness Test
CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation If either setting is turned off, the following warnings will appear: Step 2: First-Time Use contd. See the Firmware Update Procedure section for more details. Troubleshooting section if an error occurs during the first-time setup protocol.
Page 23 CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation c. Remove the blank, blue gasket from the shipping/readiness test block. Step 3: Readiness Test contd. d. Hold the block from one side and gradually apply force to lift the block from the tray.
Page 24: Step 4: Main One-Touch Operation
CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation Step 4: The home screen indicates the instrument is ready for use. There are several key functions that provide valuable information. Main One-Touch Operation Clock Status Icons 16:55 Firmware v2.1.0...
Page 25 CG000396 | Rev K User Guide | Chromium X Series Instrument | System Operation e. At the end of a run, the button will display an eject symbol and a Step 4: “Run Info” tab at the bottom of the screen. Green indicates a Main One-Touch successful completed run.
Page 26: Best Practices
Tips & Best Practices Chromium Next GEM Chip Chip Placement on Chromium X Tray 10x Genomics Cloud...
Page 27: Tips & Best Practices
CG000396 | Rev K User Guide | Chromium X Series Instrument | Tips & Best Practices Icons Tips & Best Signifies critical step Troubleshooting HT specific protocol GEM-X specific Practices section requiring accurate section includes step updates protocol includes additional...
Page 28 CG000396 | Rev K User Guide | Chromium X Series Instrument | Tips & Best Practices Chip Handling Chromium X Series Chip Holders contd. • Chromium X Series Chip Holders encase both Chromium Next GEM Chips used for HT (high throughput assay) and Chromium GEM-X Chips.
Page 29 CG000396 | Rev K User Guide | Chromium X Series Instrument | Tips & Best Practices Chip Loading Chip Handling contd. • Place the assembled chip and holder flat (gasket attached) on the bench with the lid open. • Dispense at the bottom of the wells without introducing bubbles.
Page 30: Chip Placement On Instrument Tray
CG000396 | Rev K User Guide | Chromium X Series Instrument | Tips & Best Practices Indentations and locater pins assist in proper placement of the Chip Placement on assembled chip and holder on the instrument tray. Instrument Tray Finger Access...
Page 31: Menu Options
Menu Options Menu Options Firmware Update Procedure...
Page 32 CG000396 | Rev K User Guide | Chromium X Series Instrument | Menu Overview Menu Options The menu options page can be used to access run logs, configure network connectivity, and access system settings. 16:55 Firmware v2.1.0 16:55 Firmware v2.1.0 16:55 Firmware v2.1.0...
Page 33 CG000396 | Rev K User Guide | Chromium X Series Instrument | Menu Overview To review run logs with 10x Genomics Technical Support: Menu Options contd. • Email Support@10xgenomics.com with any applicable error codes. • Upload pertinent logs directly to 10x Cloud or via USB.
Page 34: Network & Security
Genomics support team to quickly respond to issues, Diagnostics is enabled after software updates. Network & Security Allow outbound TCP port 443 for 10x Genomics Cloud access. As an additional (optional) level of security, the instrument can be put on its own network VLAN with Internet-only access.
Page 35 CG000396 | Rev K User Guide | Chromium X Series Instrument | Menu Overview Menu Options System contd. The system runs on a Linux platform. The instrument’s hardware and software have been securely implemented to prevent modification and to only execute trusted 10x code. There is no end-user configuration or access to the internal hardware, OS, or applications.
Page 36: Firmware Update Procedure
CG000396 | Rev K User Guide | Chromium X Series Instrument | Menu Overview Step 1: Download and install the update Firmware Update Procedure Procedure with an instrument connected to the internet and auto-download enabled (recommended): Automatic downloads of updates are enabled after firmware updates to ensure the instrument is up-to-date with the latest assay products, features, and bug fixes.
Page 37: Maintenance
Maintenance System Maintenance Moving & Shipping the Instrument...
Page 38: Environmental Requirements
10x Genomics service personnel. Environmental Requirements It is the design intent of the Chromium X Series instrument that it be used in a typical indoor laboratory environment. The instrument’s operating temperature is 18–28°C (64–82°F), Humidity 80% Max (Non- 10xgenomics.com...
Page 39 Turn off the instrument completely using the power switch on the back of the instrument. e. Remove the power cord. DO NOT turn on the Chromium X Series instrument again after inserting the shipping/readiness test block. It will raise the manifold into a position unsafe for shipping.
Page 40 CG000396 | Rev K User Guide | Chromium X Series Instrument | Maintenance Moving & Shipping Step 3: Packaging Diagram (for shipping) the Instrument contd. Chromium X Series Instrument Inner Box Inner Box Outer Box Link to TOC | 10xgenomics.com...
Page 41: Troubleshooting
Troubleshooting Error Screens Pressure Release Valve Fluidics Flush Mode...
Page 42 Support@10xgenomics.com with the error code displayed. Support will request log files for troubleshooting. Upload pertinent logs to 10x Genomics by navigating to the Logs menu option on screen. (See Logs section).
Page 43: Pressure Release Valve
Valve unresponsive. DO NOT open the Chromium X Series instrument. There are no user- serviceable parts inside. Refer all servicing to qualified 10x Genomics service personnel. Link to TOC |...
Page 44: Appendix
Appendix Discontinued Kits...
Page 45 The following kits have been discontinued. Their components have Discontinued Kits the same functionality and can be used interchangeably with those in the Chromium X Series Accessory Kit (PN-1000821) and the GEM-X Transition Kit (PN-1000707). Verify by part number. The Chromium X Series Accessory Kit (PN-1000821) was formerly known as the Chromium X/iX Accessory Kit (PN-1000709), with updated item names.

This manual is also suitable for:

Chromium x Chromium ix Chromium xo Pn-1000331 Pn-1000332 Pn-1000328 ... Show all