10x Genomics Chromium X Series User Manual
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10x Genomics Chromium X Series User Manual

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User Guide | CG000396 | Rev G
Chromium X Series (X/iX)
with Readiness Test
For use with:
Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium iX & Accessory Kit, 12-Month Warranty, PN-1000328
Chromium iX Accessory Kit, PN-1000323
Chromium iX & Accessory Kit, 24-Month Warranty, PN-1000329
Chromium iX Accessory Kit, PN-1000323
Chromium iX/X Refurbished, PN-1000326R
10xgenomics.com

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Summary of Contents for 10x Genomics Chromium X Series

  • Page 1 User Guide | CG000396 | Rev G Chromium X Series (X/iX) with Readiness Test For use with: Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331 Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327 Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332 Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327 Chromium iX &...
  • Page 2 © 2023 10x Genomics, Inc. (10x Genomics). All rights reserved. Duplication and/or reproduction of all or any portion of this document without the express written consent of 10x Genomics, is strictly forbidden. Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any products described herein.
  • Page 3 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Document Revision Summary Document Document Number CG000396 | Rev G Revision Summary Title Chromium X Series (X/iX) User Guide with Readiness Test Revision Rev F to Rev G...

  • Page 4: Table Of Contents

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Table of Contents Table of Introduction Introduction Contents Product Identification Label Product Specifications Safety & Compliance Information Safety Regulatory System Components System Components Accessory Kits System Operation Step 1: Installation...

  • Page 5: Introduction

    Introduction Introduction Product Identification Product Specifications...
  • Page 6 • Standard assay: 500-10,000 cells per channel 500-30,000 cells per channel with CellPlex The Chromium X Series is available in the X and iX configurations. Both the standard Next GEM chip and holder (silver color) and the HT Next GEM chip and holder (black color) are compatible with the instrument.
  • Page 7 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Introduction Product Identification Label Image of label for reference only Product Parameter Specifications Specifications Weight 41.4 lb (18.8 kg) Instrument Dimensions with: Tray close 12.2”[31.0 cm] × 18.6”[47.2 cm] × 10.2”[26.0 cm] Tray open 12.2”[31.0 cm] ×...

  • Page 8: Safety & Compliance Information

    Safety & Compliance Information Safety Regulatory...

  • Page 9: Safety

    Pinch risk: Ensure no obstructions or fingers present near closing tray. Warning: Avoid using the Chromium X/iX in a manner not specified by 10x Genomics. The Chromium X/iX has been designed to protect the user. If used improperly, the intended user protections can be impaired.

  • Page 10: Regulatory

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Safety & Compliance Information The Chromium X Series has been designed, tested, and certified to be in Regulatory compliance with the following standards: Certification Standards TUV Certification only for Chromium X/iX UL 61010-1:2012 and CAN/CSA C22.2 No.

  • Page 11: System Components

    System Components System Components Chromium X/iX Accessory Kits...
  • Page 12 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Components System Components Front Touchscreen Tray Tray Finger Access Area Indentations for chip holder feet Locater Pins Rim for chip holder placement Back USB Ports Power Supply...

  • Page 13: Accessory Kits

    * Blank gaskets are meant for use only with shipping/readiness test block and are not compatible with the secondary chip holder. † The Chromium X Series instrument is compatible with VFAT, FAT32, and exFAT formatted USBs. ‡ A shipping/readiness test block and blank gasket assembly is located on the tray within the instrument during shipping.

  • Page 14: System Operation

    System Operation Step 1: Installation Step 2: First-Time Use Step 3: Readiness Test Main One-Touch Operation...

  • Page 15: Step 1: Installation

    Warning: Avoid using the Chromium X/iX in a manner not specified by 10x Genomics. The Chromium X/iX has been designed to protect the user. If used improperly, the intended user protections can be impaired. General Power Safety Grounding is required to prevent electric shock.
  • Page 16 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation Step 1: Unbox Installation contd. DO NOT POWER ON UNTIL READY FOR FIRST-TIME USE SETUP Chromium X Upgrade Kit Chromium iX (only present Accessory Kit when X upgrade...
  • Page 17 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation Step 1: Remove Shipping Material from Tray Installation The instrument may now include a shipping lock that further secures and stabilizes internal contd. components to prevent damage during shipping. See...

  • Page 18: Step 2: First-Time Use

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation Step 2: The instrument will walk through a first-time setup protocol. Setup requires the following: First-Time Use • 10x Genomics Account Onscreen instruction will guide account setup. Alternatively, visit cloud.
  • Page 19 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation d. Register the instrument. Step 2: First-Time Use • Navigate to register.10xgenomics.com using the onscreen QR code on a contd. computer or phone with internet access. Create or log in to a 10x Genomics account.
  • Page 20 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation After the instrument updates, a recommended configuration screen Step 2: will appear. On this screen, the ability of the instrument to First-Time Use automatically upload diagnostic data as well as automatically contd.

  • Page 21: Step 3: Readiness Test

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation The Readiness Test verifies that all systems are working optimally. The Step 3: first-time setup protocol will prompt the initial readiness test: Readiness Test DO NOT start the Readiness Test until all shipping materials, including the shipping lock, block, and tape, have been removed.

  • Page 22: Step 4: Main One-Touch Operation

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation f. Reinstall blank gasket over the block to prepare for the Readiness Test. Step 3: Readiness Test • Ensure that the gasket is properly attached (see 10x Gasket Attachment).

  • Page 23: Tips & Best Practices

    CG000396 | Rev G User Guide | Chromium X Series (X/iX) | System Operation Step 4: Main Operation Main One-Touch a. Press eject button on the touchscreen to eject the tray. A chime will Operation sound. contd. b. Load an assembled chip and holder (see Tips &...

  • Page 24: Chromium Next Gem Chip

    Tips & Best Practices Chromium Next GEM Chip Chip Placement on Chromium X Tray 10x Genomics Cloud...
  • Page 25 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Tips & Best Practices Icons Tips & Best Practices section Signifies critical step Troubleshooting section HT specific protocol includes additional guidance requiring accurate execution includes additional guidance step updates Chromium For instuctions on the standard assay Next GEM chip, refer to the appropriate assay kit user guide.
  • Page 26 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Tips & Best Practices Chromium Chip & Holder Assembly Next GEM Chip contd. Chip in Chromium X Secondary Holder Chromium X Secondary Holder Assembled with Gasket Chip Clip •...
  • Page 27 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Tips & Best Practices 10x Gasket Attachment Chromium • Before reagents are loaded, attach the gasket by holding the tongue Next GEM Chip (curved end, to the right) and hook the gasket on the left-hand tabs of contd.

  • Page 28: Menu Options

    Menu Options Menu Options Firmware Update Procedure...
  • Page 29 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Menu Overview Menu Options The menu options page can be used to access run logs, configure network connectivity, and access system settings. Power off the instrument by first performing a soft power down, by holding the power icon at the bottom of the menu options screen.
  • Page 30 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Menu Overview To review run logs with 10x Genomics Technical Support: Menu Options contd. • Email Support@10xgenomics.com with any applicable error codes. • Upload pertinent logs via USB (no network connection) or directly to 10x Cloud if you are connected to the Internet.
  • Page 31 Network & Security Menu Options contd. Allow outbound TCP port 443 for 10x Genomics Cloud access. As an additional (optional) level of security, the instrument can be put on its own network VLAN with Internet-only access. For connectivity to work correctly, the network should allow unrestricted outbound access to the following hosts: •...

  • Page 32: Firmware Update Procedure

    Firmware updates are linked to specific instruments. If the chosen account is linked to multiple instruments, select the correct download link for the desired serial number. The Chromium X Series instrument is compatible with VFAT, FAT32, and exFAT formatted USBs.
  • Page 33 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Menu Overview Step 2: Reinitialize the instrument Firmware Update Procedure After installing an update, run a Readiness Test. See Readiness Test section for further contd. guidance. a. Retrieve the shipping/readiness test block with attached blank gasket.

  • Page 34: Maintenance

    Maintenance System Maintenance Moving & Shipping the Instrument...
  • Page 35 Interior Maintenance The Chromium X Series tray and door areas have been designed to catch and contain drips and a small volume of liquid spills. Occasionally, use a soft lab towel to clean these areas with a mixture of mild detergent and distilled water.
  • Page 36 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Maintenance The Chromium X/iX utilizes a shipping mode for safe instrument Moving & Shipping transportation. Shipping mode can be launched through the Alternate the Instrument Modes menu option. The shipping/readiness test block and a blank gasket are required for transporting the Chromium X/iX in shipping mode, even for short distances (e.g., from one bench to another).
  • Page 37 CG000396 | Rev G User Guide | Chromium X Series (X/iX) | Maintenance Moving & Shipping Step 3: Packaging Diagram (for shipping) the Instrument contd. Chromium X Series Instrument Inner Box Inner Box Outer Box Link to TOC | 10xgenomics.com...

  • Page 38: Troubleshooting

    Troubleshooting Error Screens...
  • Page 39 Support@10xgenomics.com with the error code displayed. Support will request log files for troubleshooting. Upload pertinent logs to 10x Genomics by navigating to the Logs menu option on screen. (See Logs section).

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