10x Genomics Chromium X Series User Manual
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User Guide | CG000396 | Rev C
Chromium X Series (X/iX)
with Readiness Test
For use with:
Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332
Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327
Chromium iX & Accessory Kit, 12-Month Warranty, PN-1000328
Chromium iX Accessory Kit, PN-1000323
Chromium iX Accessory Kit, 24-Month Warranty, PN-1000329
Chromium iX Accessory Kit, PN-1000323
10xgenomics.com
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Table of Contents
Related Manuals for 10x Genomics Chromium X Series
Summary of Contents for 10x Genomics Chromium X Series
- Page 1 User Guide | CG000396 | Rev C Chromium X Series (X/iX) with Readiness Test For use with: Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331 Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327 Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332 Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327 Chromium iX &...
- Page 2 © 2021 10x Genomics, Inc. (10x Genomics). All rights reserved. Duplication and/or reproduction of all or any portion of this document without the express written consent of 10x Genomics, is strictly forbidden. Nothing contained herein shall constitute any warranty, express or implied, as to the performance of any products described herein.
- Page 3 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Document Revision Summary Document Document Number CG000396 | Rev C Revision Summary Title Chromium X Series (X/iX) User Guide with Readiness Test Revision Rev B to Rev C...
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Page 4: Table Of Contents
CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Table of Contents Table of Introduction Introduction Contents Product Identification Label Product Specifications Safety & Compliance Information Safety Regulatory System Components System Components Accessory Kits System Operation Step 1: Installation... -
Page 5: Introduction
Introduction Introduction Product Identification Product Specifications... - Page 6 • Standard assay: 500-10,000 cells per channel 500-30,000 cells per channel with CellPlex The Chromium X Series is one instrument, available in two firmware options (Chromium X and Chromium iX). Both the standard Next GEM chip and holder (silver color) and the HT Next GEM chip and holder (black color) are compatible with the instrument.
- Page 7 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Introduction Product Identification Label Image of label for reference only Product Parameter Specifications Specifications Weight 41.4 lb (18.8 kg) Instrument Dimensions with: Tray close 12.2”[31.0 cm] × 18.6”[47.2 cm] × 10.2”[26.0 cm] Tray open 12.2”[31.0 cm] ×...
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Page 8: Safety & Compliance Information
Safety & Compliance Information Safety Regulatory... -
Page 9: Safety
Pinch risk: Ensure no obstructions or fingers present near closing tray. Warning: Avoid using the Chromium X/iX in a manner not specified by 10x Genomics. The Chromium X/iX has been designed to protect the user. If used improperly, the intended user protections can be impaired. -
Page 10: Regulatory
CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Safety & Compliance Information The Chromium X Series has been designed, tested, and certified to be in Regulatory compliance with the following standards: Certification Standards TUV Certification only for Chromium X/iX UL 61010-1:2012 and CAN/CSA C22.2 No. -
Page 11: System Components
System Components System Components Chromium X/iX Accessory Kits... - Page 12 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | System Components System Components Front Touchscreen Tray Tray Finger Access Area Indentations for chip holder feet Locater Pins Rim for chip holder placement Back USB Ports Power Supply...
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Page 13: Accessory Kits
Chip Gasket, HT, 5 Pack 3000614 10x Magnetic Separator HT 2000431 USB, 8 GB* 3000664 *The Chromium X Series instrument is compatible with VFAT and exFAT formatted USBs. Additional Items Product Part Number (Item) Shipping/Readiness Test Block 3000652 Chip Gasket, HT... -
Page 14: System Operation
System Operation Installation First-Time Use Readiness Test Main One-Touch Operation... -
Page 15: Step 1: Installation
Warning: Avoid using the Chromium X/iX in a manner not specified by 10x Genomics. The Chromium X/iX has been designed to protect the user. If used improperly, the intended user protections can be impaired. Link to TOC |... - Page 16 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | System Operation Step 1: Unbox Installation contd. DO NOT POWER ON UNTIL READY FOR FIRST-TIME USE SETUP Chromium X Chromium iX Upgrade Kit Accessory Kit (only present when X upgrade...
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Page 17: Step 2: First-Time Use
Walk through the Internet Connectivity Setup (optional) and the Date and Time Setup. d. On a networked device, login to your 10x Genomics account at activate.10xgenomics.com and register the instrument. Enter the serial number + instrument ID. Read and accept the legal agreement. -
Page 18: Step 3: Readiness Test
CG000396 | Rev C User Guide | Chromium X Series (X/iX) | System Operation The readiness test verifies that all systems are working optimally. The Step 3: first-time setup protocol will prompt the initial readiness test: Readiness Test a. Follow screen instruction to launch the readiness test. -
Page 19: Step 4: Main One-Touch Operation
CG000396 | Rev C User Guide | Chromium X Series (X/iX) | System Operation Step 4: The home screen indicates the instrument is ready for use. This is the most common state of the instrument. There are several key functions Main One-Touch that provide valuable information. - Page 20 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | System Operation j. At the end of a run, the button will display an eject symbol and a “Run Info” tab at the bottom of the screen. • Green indicates a successful completed run.
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Page 21: Chromium Next Gem Chip
Tips & Best Practices Chromium Next GEM Chip Chip Placement on Chromium X Tray 10x Genomics Cloud... - Page 22 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Tips & Best Practices Icons Tips & Best Practices section Signifies critical step Troubleshooting section HT specific protocol includes additional guidance requiring accurate execution includes additional guidance step updates Chromium For instuctions on the standard assay Next GEM chip, refer to the appropriate assay kit user guide.
- Page 23 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Tips & Best Practices Chromium Chip & Holder Assembly Next GEM Chip contd. Chip in Chromium X Secondary Holder Chromium X Secondary Holder Assembled with Gasket Chip Clip •...
- Page 24 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Tips & Best Practices 10x Gasket Attachment Chromium • Before reagents are loaded, attach the gasket by holding the tongue Next GEM Chip (curved end, to the right) and hook the gasket on the left-hand tabs of contd.
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Page 25: Menu Options
Menu Options Menu Options Firmware Update Procedure... - Page 26 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Menu Overview Menu Options Main mode of access to Logs, Connectivity, and System information. Power off the instrument by tapping the power icon at the bottom of the menu options screen.
- Page 27 Internet connectivity is not required, but is highly recommended so that the instrument may receive security/firmware updates and automatically upload system log files to the 10x Genomics Cloud. 10x Genomics has no remote access to the instrument. Users may separately configure connectivity settings related to firmware updates and log upload behavior.
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Page 28: Firmware Update Procedure
CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Menu Overview Step 1: Download and install the update Firmware Update Procedure Procedure with a network connection, auto-download enabled: a. A pop-up on the home screen will alert the user of available updates. -
Page 29: Maintenance
Maintenance System Maintenance Moving & Shipping the Instrument... - Page 30 Interior Maintenance The Chromium X Series tray and door areas have been designed to catch and contain drips and a small volume of liquid spills. Occasionally, use a soft lab towel to clean these areas with a mixture of mild detergent and distilled water.
- Page 31 CG000396 | Rev C User Guide | Chromium X Series (X/iX) | Maintenance System Environmental Requirements Maintenance It is the design intent of the Chromium X/iX that it be used in a typical contd. indoor laboratory environment. The instrument’s operating temperature is 18–28°C (64–82°F), Humidity 80% Max (Non-Condensing) Altitude...
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Page 32: Troubleshooting
Troubleshooting Error Screens... - Page 33 Support@10xgenomics.com with the error code displayed. Support will request log files for troubleshooting. Upload pertinent logs to 10x Genomics by navigating to the Logs menu option on screen. (See Logs section).
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