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Microm Rotary Microtome HM 325 Service Manual

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Rotary Microtome HM 325
SERVICE MANUAL
ROTARY MICROTOME
HM 325
from Ser. No. 21265 up
MICROM International GmbH
Robert-Bosch-Str. 49
D-69190 Walldorf
391 140 - English

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Summary of Contents for Microm Rotary Microtome HM 325

  • Page 1 Rotary Microtome HM 325 SERVICE MANUAL ROTARY MICROTOME HM 325 from Ser. No. 21265 up MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 2 Rotary Microtome HM 325 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...

  • Page 3: Table Of Contents

    Rotary Microtome HM 325 Table of contents Part 1 Introduction Part 2 Description of the Rotary Microtome HM 325 Accessories 2-1-1 Standard equipment 2-1-2 Additional equipment, optional Part 3 Technical specifications HM 325 Part 4 Theory of design and operation...
  • Page 4 Rotary Microtome HM 325 Part 9 Exchanging and adjusting assemblies and spare parts Section waste tray Specimen clamps and specimen clamping Handwheels and handwheel brake Coarse feed Trimming function, knob for setting section thickness Housing Retraction mechanism Shaft with bearing, link block µm-setting...

  • Page 5: Introduction

    This MICROM product is warranted against defects in material and workmanship for a period of 1 year. Parts which prove to be defective during the warranty period will be repaired or replaced free of charge by MICROM International GmbH. No other warranty expressed implied.

  • Page 6: Description Of The Rotary Microtome Hm 325

    Part 2 Description of the Rotary Microtome HM 325 The Rotary Microtome HM 325 from MICROM International GmbH is a highly efficient instrument which can be used for paraffin sectioning as well as for the hard sectioning technique in biology, medicine and industry.

  • Page 7: Accessories

    Rotary Microtome HM 325 Accessories 2-1-1 Standard equipment The microtome HM 325 is supplied with the following accessories: 1 Hex head wrench 6 mm 1 Cover 1 Para Gard, 100 ml 1 Instruction manual 2-1-2 Additional equipment (optional) 2-1-2-1 Knife carrier Cat.
  • Page 8 Rotary Microtome HM 325 2-1-2-3 Optical accessories Cat. No. Large field magnifier, 220 V 760160 Large field magnifier, 120 V 760170 Stereomicroscope Stemi 1000 755080 Adapter for stereomicroscope Stemi 1000 532080 Illumination devices: KL 750 with ring light 230 V/50 - 60 Hz...
  • Page 9 Rotary Microtome HM 325 2-1-2-5 Additional accessories Cat. No. Fast freezing unit K 300 100 V/50 - 60 Hz 770080 115 V/60 Hz 770070 230 V/50 Hz 770030 240 V/50 Hz 770090 Automatic approach system 604120 Storage unit 520450 Section transfer system with knife carrier T...

  • Page 10: Technical Specifications Hm 325

    Rotary Microtome HM 325 Part 3 Technical specifications HM 325 Microtome: Section thickness range: 0,5 - 60 µm Resolution: 0,5 µm for 0,5 - 2 µm 1 µm for 2 - 10 µm 2 µm for 10 - 20 µm 5 µm for 20 - 60 µm...

  • Page 11: Theory Of Design And Operation

    Theory of design and operation Mechanical design The Rotary Microtome HM 325 is designed as a bench unit (fig. 1) with removable section waste tray (fig. 1.2) and an enclosed housing (fig. 1.1) made of polyurethane. The section waste tray (fig.

  • Page 12: Functional Description

    Rotary Microtome HM 325 Fig. 2 RESET pin for section counter Section counter Carrier plate Trimming key 4-2 Functional description 4-2-1 Microtome with retraction To start the cutting movement of the microtome, turn the handwheel. To balance the vertically moving mass, leaden counterweights are installed in the handwheel. The rotary drive movement is converted into a vertical movement of the horizontal cylinder (fig.
  • Page 13 Rotary Microtome HM 325 The selected thickness is delivered at the upper reversal point of the vertical movement. The horizontal cylinder together with the specimen moves forwards by the value of the section thickness, which can be selected on the knob (fig. 1.7). This value is shown on the indexed wheel (fig.
  • Page 14 Rotary Microtome HM 325 With the upward movement of the monobloc (fig. 5.2), the setting arm (fig. 5.11), which is placed on the rear end, touches the cam disk (fig. 5.3) and is then moved downwards by a certain angle. If the setting arm (fig. 5.11) with holding ring is moved downwards, the rollers (fig.
  • Page 15 Rotary Microtome HM 325 Fig. 3 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 16 Rotary Microtome HM 325 Fig. 4 1 = Bolt 4 = Slide hook 2 = Link block 5 = Crank arm 3 = Monobloc 6 = Driving axle handwheel MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 17 Rotary Microtome HM 325 Fig. 5 1 = Frame 12 = Toothed wheel (54 teeth) 2 = Monobloc 13 = Positioning pin 3 = Cam disk 14 = Micro switch 4 = Shaft with bearing 15 = Adjusting pin 5 = Pressure spring...

  • Page 18: Specimen Coarse Feed And Trimming Stages

    Rotary Microtome HM 325 Fig. 6 Micrometer spindle Micrometer nut 4-2-2 Specimen coarse feed and trimming stages After changing the specimen, moving the knife or knife carrier, it is necessary to adjust the specimen to the knife edge again. This can easily be done by means of the specimen coarse feed with trimming stage.
  • Page 19 Rotary Microtome HM 325 The movement of the handwheel (fig. 5.19) for the coarse feed cannot be used as a criterium for perfect feed settings, as the toothed wheels and links have play. The movement of the coarse feed is limited. When the front or back end position of the specimen feed is reached, clear resistances can be noticed.

  • Page 20: Functional Description Of The Trimming Function

    Rotary Microtome HM 325 4-2-3 Functional description of the trimming function The feed can be carried out in graduated trimming stages. Trimming stages of 10 µm and 30 µm are available. For trimming stage 1 (10 µm), press the trimming key (fig. 1.4) down until a first resistance can be felt and turn the handwheel clockwise at the same time.

  • Page 21: Maintenance Methodology

    Maintenance level I can be performed by the user himself and is exactly described in the instruction manual. Maintenance level III must be performed in the factory of MICROM International GmbH in Walldorf. For this reason, it is necessary to return the rotary microtome in its original packing.

  • Page 22: Field Maintenance Technique

    Rotary Microtome HM 325 Field maintenance techniques Service and maintenance work listed in level II are carried out by a trained service technician. A routine maintenance should be performed once a year: 5-2-1 Works on the microtome: Check the movable parts for good surface quality, dimensional accuracy and wear and tear.

  • Page 23: Assembly And Spare Parts List

    Rotary Microtome HM 325 Part 6 Assembly and spare parts list The below listed assemblies and parts may only be replaced by trained service technicians. Detailed descriptions of the replacement work can be found in part 8 and 9 of this service manual.
  • Page 24 Rotary Microtome HM 325 Fig. no. Description Cat. No. ______________________________________________________________________________ 3.26 Bearing arm 438120 3.27 Pinion for feed setting 466230 3.28 Bearing flange (see 3.9, shaft with bearing) Bolt Link block 421050 Monobloc (horiz. cylinder with guidance) 518240 Slide hook...
  • Page 25 Rotary Microtome HM 325 Fig. No. Description Cat. No. ______________________________________________________________________________ 12.7 Eccenter 405080 12.8 Groove 12.9 a. 12.10 Insertion with handle 424800 12.11 Fastening screw M4x25 13.1 Fastening housing 13.2 Slide feet 454080 13.3 Hole fastening adjusting screw handwheel brake 13.4...
  • Page 26 Rotary Microtome HM 325 Fig. No. Description Cat. No. ______________________________________________________________________________ 17.9 Thread for fastening dial gauge holder 17.10 Cover ribbon, complete 510170 17.11 Deflection pulley 18.1 Carrier plate 410310 18.2 Section counter board 616010 18.3 Threaded bolt and fastening nut M2 18.4...
  • Page 27 Rotary Microtome HM 325 Fig. No. Desription Cat. No. ______________________________________________________________________________ 20.25 Cylinder pin 2,5m6x16(14) 20.26 Toothed wheel (54 teeth) 466070 20.27 Threaded bush 14x19 414050 21.1 Horizontal cylinder (horiz. cylinder with guidance) 518240 21.2 Fastening dial gauge holder 21.3 Retaining washer 2,3 21.4...
  • Page 28 Rotary Microtome HM 325 Fig. No. Description Cat. No. ______________________________________________________________________________ 23.9 Hole to adjusting screws 23.10 Plastic ring 24.1 Cover ribbon, complete 510170 24.2 Horizontal cylinder 24.3 Tension spring 24.4 Spring hook 428050 24.5 Insert 24.6 Deflection pulleys 444190 24.7...
  • Page 29 Rotary Microtome HM 325 Necessary spare parts, which are not included in a figure. Cat. No. Description ______________________________________________________________________________ 350010 Oil, 100 ml 350140 ISO Flex grease 350190 Loctite Nr. 638, green, 10 ml 350200 Loctite Nr. 222e, pink, 10 ml 350210 Loctite Nr.

  • Page 30: Tools And Equipment

    Rotary Microtome HM 325 Part 7 Tools and equipment To carry out repair or replacement work, the below-mentioned tools as well as a set of standard tools are required. Description Cat. No. ___________________________________________________________________________ Torque wrench 362060 Dial gauge 362070 Dial gauge holder...
  • Page 31 Rotary Microtome HM 325 Fig. 7 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...

  • Page 32: Fault Diagnostics

    Rotary Microtome HM 325 Part 8 Fault diagnostics This part includes some general guidelines and hints on how to define a service problem and to replace the defective assembly or part. General techniques To determine malfunctions, good knowledge and understanding of the architecture of the rotary microtome is required.

  • Page 33: Error Code Diagnostics

    Rotary Microtome HM 325 Error code diagnostics Faults Cause Removal Ref. Thick-thin-sections sharpness of knife check, resharpen knife angle check, adjust Clampings on knife carrier check, adjust Clampings on specimen clamping check, adjust micrometer play adjust micrometer nut 9-11 cross roller bearings...
  • Page 34 Rotary Microtome HM 325 Faults Cause Removal Ref. Inaccurate feed cross roller bearings check, clean, lubricate, vertical adjust, 6 Ncm free-wheel not exact check, clean 9-11 play between nut and adjust micrometer nut 9-11 spindle play in spindle bearing check springs in thrust ring,...

  • Page 35: Adjustment Of Knives

    Rotary Microtome HM 325 Adjustment of knives According to the facette of C and D knives select a clearance angle of 5 - 15°and note the correct setting of the knife height. For disposable blades select a clearance angle of 10 - 12°.

  • Page 36: Knife Carrier S

    Rotary Microtome HM 325 8-4-2 Knife carrier S Readjusting the clamping lever of the knife carrier S can also become necessary. If the clamping lever is in an unfavourable position, i.e. not in a 45° position, remove the knife carrier. Loosen or tighten the set screw on the bottom of the knife carrier to change the clamping position of the lever.
  • Page 37 Rotary Microtome HM 325 Adjusting the cross roller bearings The adjustment of the cross roller bearings is carried out via the rail fastened on the left side of the frame. However, the other three guide rails must be inserted properly and the screws must securely be tightened.

  • Page 38: Link Block

    Rotary Microtome HM 325 Link block To clean the link block, remove the shaft with bearing (see part 9-8) and the handwheel (see part 9-3). After having removed the shaft with bearing, the link block can either be dismounted from the crank pin (fig.

  • Page 39: Section Waste Tray

    Rotary Microtome HM 325 Part 9 Exchanging and adjusting assemblies and spare parts Section waste tray The section waste tray (fig. 1.2) is made of polyurethane. Polyurethane is a robust material, which can be damaged by heavy impacts from outside.
  • Page 40 Rotary Microtome HM 325 Fig. 11 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 41 Rotary Microtome HM 325 Handwheels and handwheel brake When doing service on the rotary microtome HM 325 and it is necessary to remove the housing of the instrument, the handwheels for the cutting drive as well as for the coarse feed and the operating lever for the handwheel brake must be dismounted.
  • Page 42 Rotary Microtome HM 325 Fig. 12 Handle 6 = Pressure spring 11 = Fastening screw Adjusting screw 7 = Eccenter Thrust bolt 8 = Groove Rubber buffer 9 = Insertion for handle Plain washer 10 = Handle MICROM International GmbH Robert-Bosch-Str.
  • Page 43 Rotary Microtome HM 325 Coarse feed To carry out service work on the coarse feed, first remove the housing (fig. 1.1) (see part 9-6). Loosen the fastening screw of the bearing support (fig. 5.22) from below. Pull the bearing support (fig. 5.22) with link axle coarse feed (fig. 5.9) and kelly off the kelly sparing of the extension arm (fig.
  • Page 44 Rotary Microtome HM 325 Housing The housing (fig. 1.1) is made of polyurethane. Polyurethane is a robust material, which can be damaged by heavy impacts from outside. To remove the housing (fig. 1.1), first dismount the section waste tray (fig. 1.2) (see part 9-1), the specimen clamp with specimen clamping (fig.
  • Page 45 Rotary Microtome HM 325 Retraction mechanism To carry out service work on the retraction mechanism, first remove the housing (fig. 1.1) (see part 9-6): Removal: Loosen the allen screw (fig. 14.10) and remove the brake spring (fig. 14.6). Loosen the threaded pin (fig.
  • Page 46 Rotary Microtome HM 325 Fig. 14 1 = Stop pin 6 = Brake spring 11 = Pin 2 = Leverage head 7 = Monobloc 3 = Cylinder pin 8 = Threaded pin M3x4(5) 4 = Switch segment 9 = Eccentric bush...
  • Page 47 Rotary Microtome HM 325 Shaft with bearing, link block To carry out service work on the shaft with bearing, first remove the handwheel (fig. 1.8) (see part 9-3). First unhinge the spring (fig. 15.15) for the first trimming stage. Loosen the two fastening screws of the stop lever (fig.
  • Page 48 Rotary Microtome HM 325 Fig. 15 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 49 Rotary Microtome HM 325 Fig. 16 1 = Crank arm 6 = Flange sleeve 2 = Ball bearing 7 = Clamping pin 3 = Flange 8 = Clamping pin 4 = Shaft 9 = Hole 5 = Ball bearing 10 = Crank pin MICROM International GmbH Robert-Bosch-Str.
  • Page 50 Rotary Microtome HM 325 µm-setting To carry out service work on the µm-setting, first remove the housing (fig. 1.1) (see part 9-6). Loosen the fastening screws of the bloc (fig. 3.26). Then remove the bloc (fig. 3.26) together with the pinion for feed setting (fig. 3.27) and the stop spring (fig. 3.25). Loosen the threaded pins on the cam disk (fig.
  • Page 51 Rotary Microtome HM 325 Fig. 17 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...

  • Page 52: Control Panel

    Rotary Microtome HM 325 9-10 Control panel To carry out service work on the control panel, first remove the housing (fig. 1.1) (see part 9-6). Unscrew the five nuts from the threaded bolts (fig. 18.5) and remove the control panel from the housing.
  • Page 53 Rotary Microtome HM 325 Fig. 19 1 = Carrier plate 7 = Tension spring 2 = Section counter, complete 8 = Fastening housing on microtome 3 = Housing 9 = Connection micro switch 4 = Lithium battery 3 V 10 = Connection micro switch...
  • Page 54 Rotary Microtome HM 325 9-11 Micrometer mechanism To dismount the micrometer mechanism, unhinge the tension spring (fig. 5.17) from the groove pin with gorge (fig. 24.18) on the setting arm (fig. 20.17). Keep the micrometer spindle (fig. 20.10) with the special key (fig. 7, cat. no. 362100) and loosen the threaded bush (fig. 20.27) with the special key Fig.
  • Page 55 Rotary Microtome HM 325 Fig. 20 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 56 Rotary Microtome HM 325 Fig. 21 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 57 Rotary Microtome HM 325 9-12 Guide wedges and horizontal cylinder To avoid turning the horizontal cylinder (fig. 22.2), guide wedges are inserted in the monobloc (fig. 22.1). These guide wedges grip into the groove (fig. 22.3) of the horizontal cylinder. Two guide wedges are placed one behind the other and are slightly laterally offset.
  • Page 58 Rotary Microtome HM 325 Fig. 22 1 = Monobloc 2 = Horizontal cylinder 3 = Groove 4 = Rear guide wedge 5 = Front guide wedge 6 = Thread 7 = Spring 8 = Peg 9 = Pin MICROM International GmbH Robert-Bosch-Str.
  • Page 59 Rotary Microtome HM 325 9-13 Cross roller bearings The cross roller bearings can only be exchanged in pairs. Disassembly: To dismount the cross roller bearings, first remove the cover ribbon (see part 9-14). The cross roller bearings consist of 2 x 2 rails each with a roller cage with rollers. One rail of each cross roller bearing pairs is screwed on the vertical sledge and on the frame.
  • Page 60 Rotary Microtome HM 325 Fig. 23 1 = Cover ledge (1 part) 2 = Deflection pulley 3 = Axle 4 = Safety washer 5 = Allen screw M3x6 6 = Cover ribbon, compl. 7 = Hook 8 = Frame 9 = Hole to adjusting screws...
  • Page 61 Rotary Microtome HM 325 Fig. 24 1 = Cover ribbon 7 = Cover strip 2 = Horizontal cylinder 8 = Not used 3 = Tension spring 9 = Frame 4 = Spring hook 10 = Monobloc 5 = Insert 11 = Holes to the adjusting screws...
  • Page 62 Rotary Microtome HM 325 9-14 Cover ribbon To exchange the cover ribboon (fig. 24.1), first remove the housing (fig. 1.1) (see part 9-6). Each end of the cover ribbon (fig. 24.1) has an insert (fig. 24.5), on which a spring (fig. 24.3) is fastened.
  • Page 63 Rotary Microtome HM 325 9-15 Acoustic signal of the front end position To carry out service work on the acoustic signal of the front end position (fig. 25), first remove the housing (fig. 1.1) (see part 9-6). To dismount the leverage support (fig. 25.3) with trigger hook (fig. 25.2), loosen the threaded pin (fig.
  • Page 64 Rotary Microtome HM 325 9-16 Adapter for stereomicroscope First check if the housing of the instrument is designed for the adaptation of a stereomicroscope. If this is not the case, exchange the housing against a new one (housing for adaptation, cat. no.
  • Page 65 Rotary Microtome HM 325 Fig. 26 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 66 Rotary Microtome HM 325 Fig. 27 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 67 Rotary Microtome HM 325 9-17 Adapter for large field magnifier First check if the housing of the instrument is designed for the adaptation of a large field magnifier. If this is not the case, exchange the housing against a new one (housing for adaptation, cat.
  • Page 68 Rotary Microtome HM 325 Fig. 28 MICROM International GmbH Robert-Bosch-Str. 49 D-69190 Walldorf 391 140 - English...
  • Page 69 Part 10 Packing and dispatch Screw the rotary microtome HM 325 from below onto the wooden plate (cat. no. 354120), belonging to the original packing, by means of two screws M10x60 with each a plain washer. Put it into the original packing and use the eight edges made of foamed material. Then place the cardboard plate on top of the instrument.

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