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Microm HM 505 E Instruction Manual

Microtome cryostat
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Microtome Cryostat HM 505 E
MICROTOME CRYOSTAT
HM 505 E
INSTRUCTION MANUAL
MICROM International GmbH
1
Robert- Bosch-Str. 49
D- 69190 Walldorf
386240 - English

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Summary of Contents for Microm HM 505 E

  • Page 1 Microtome Cryostat HM 505 E MICROTOME CRYOSTAT HM 505 E INSTRUCTION MANUAL MICROM International GmbH Robert- Bosch-Str. 49 D- 69190 Walldorf 386240 - English...
  • Page 2 Microtome Cryostat HM 505 E MICROM International GmbH Robert- Bosch-Str. 49 D- 69190 Walldorf 386240 - English...

  • Page 3: Warranty

    This instruction manual will be supplied together with each instrument. Further copies can be ordered at the nearest MICROM sales office by giving the serial number of the instrument, the number of the instruction manual and the date of issue.

  • Page 4: Intended Use

    MICROM Ser. No. :..........Please check the MICROM Ser. No. on the type plate of your instrument and enter this number here. This way, questions and service can be handled faster.
  • Page 5 Microtome Cryostat HM 505 E Intended Use Table of Contents EC Certificate of Conformity Safety Precautions Part 1 Introduction Description of the Cryostat HM 505 E Technical specifications Part 2 Operating Instructions Setting up the cryostat Initial turn-on Cooling of microtome chamber...
  • Page 6 Microtome Cryostat HM 505 E 2-10 Knife carriers 2-10-1 Standard knife carrier 2-10-2 Disposable blade carrier EC 2-11 Error code indication 2-11-1 Definition of the error code 2-12 Accessories 2-12-1 Standard equipment 2-12-2 Additional equipment (optional) Part 3 Theory of operation...
  • Page 7 DIN EN 61326/A1:1999-05 VDE 0843 Teil 20/A1:1999-05 The VDE Prüf- und Zertifizierungsinstitut (EU reference no. 0366), Merianstr. 28, D-63069 Offenbach has checked and certified the product. The marks licences entitle MICROM International GmbH to use the VDE labels opposite. Marks licence no.: Marks licence no.:...

  • Page 8: Safety Precautions

    Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. MICROM International GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements.
  • Page 9 Microtome Cryostat HM 505 E HAZARD OF FROSTBITE Avoid permanent touching of metal parts inside the cryostat microtome chamber. Frostbite at unprotected hands and arms may occur at working temperatures down to -35°C. HAZARD OF RADIOACTIVE RADIATION When working with radioactive specimens observe all applicable radiation safety procedures.
  • Page 10 Further optional accessories for the HM 505 E is the heat extractor as well as the active deep freezing device.
  • Page 11 Microtome Cryostat HM 505 E 1 - 2 TECHNICAL SPECIFICATIONS HM 505 E Cooling of microtome temperature range ............... +5°C - -35°C chamber (at +20°C ambient temp.) ................... resolution 1°C automatic defrosting .............. 1 each 24 h Microtome: Section thickness range ............1 - 500 µm Fine section thickness range .............
  • Page 12 Microtome Cryostat HM 505 E Fig. 1 PART 2 OPERATING INSTRUCTIONS The installation site must be chosen so that enough ventilation for the cooling system is guaranteed. The distance between wall and rear panel should be approx. 10 - 15 cm.

  • Page 13: Initial Turn-On

    Microtome Cryostat HM 505 E 2-2 INITIAL TURN-ON CAUTION! Before switching instrument for the first time, please check if the power requirements indicated on the type plate correspond to the power supply voltage being used. No other instruments should be connected to...
  • Page 14 Microtome Cryostat HM 505 E COOLING OF MICROTOME CHAMBER Fig. 2 2-3-1 ACTUAL AND SET VALUE OF 2-3-2 DEFROSTING THE TEMPERATURE EVAPORATOR The cooling of the microtome chamber can be Every 24 hours the evaporator in the rear part controlled by the corresponding part of the of the microtome chamber is defrosted operating controls.

  • Page 15: Cutting Movement And Retraction

    Microtome Cryostat HM 505 E The real time can be read on the display (fig. CUTTING MOVEMENT AND 2.1) if the button (fig. 2.5) is pressed in RETRACTION addition to the already pressed button DEFR TIME (fig. 2.4). To correct the real time at...
  • Page 16 Microtome Cryostat HM 505 E LOCKING AND UNLOCKING THE HANDWHEEL The function <handwheel brake> allows to lock the handwheel. A pin is situated on the rim of the handwheel, which can be pressed backwards. The handwheel is locked in the upper reversal point.
  • Page 17 Microtome Cryostat HM 505 E Fig. 4 SETTING SECTION THICKNESS AND TRIMMING THICKNESS The desired section thickness can be selected by means of the buttons (fig. 4.2) on the The graduation of the trimming thicknesses is operating controls and the trimming thickness divided into 4 ranges: by means of the buttons (fig.

  • Page 18: Specimen Coarse Feed

    Microtome Cryostat HM 505 E SPECIMEN FEED NOTE! At the beginning of a sectioning 2-7-1 SPECIMEN COARSE FEED series, it is advisable to use the coarse feed to move the specimen to the back third of the For the fast forward and backward travel...

  • Page 19: Trimming And First Cuts

    Microtome Cryostat HM 505 E Use the handwheel to make sure that the most 2-7-3 TRIMMING AND FIRST CUTS protruding position of the specimen is opposite the knife edge. After the specimen and the knife are adjusted, further gradual feed for trimming can be Press button (fig.

  • Page 20: Section Counter

    Microtome Cryostat HM 505 E 2-7-4 FINE FEED 2-8-1 SECTION COUNTER After having adjusted knife and specimen as The section counter adds up the sections well as having trimmed the specimen, produced. After each downward movement of sectioning can be started.

  • Page 21: Specimen Holder

    Microtome Cryostat HM 505 E SPECIMEN HOLDER AND Briefly press the DOWN button (fig. 2.3) SPECIMEN ORIENTATION twice to start the active deep freezing device with Peltier cooling element. The letter P is shown on the display (fig. 2.1), while the...

  • Page 22: Specimen Orientation

    Microtome Cryostat HM 505 E 2-9-3 SPECIMEN HOLDER USING 2-9-4 SPECIMEN ORIENTATION A HEAT EXTRACTOR (OPTIONAL EQUIPMENT) In many cases, the orientation of the specimen in relation to the cutting edge would be Prepare and freeze the specimen as described advantageous.
  • Page 23 The standard knife carrier of the Cryostat turn the knob (fig. 8.4) in a counter-clockwise HM 505 E is easy to use, can be moved direction. To adjust the knife edge in relation forwards or backwards and is equipped with...
  • Page 24 Microtome Cryostat HM 505 E CAUTION! The knife carrier is equipped with two knife guards (fig. 8.7) that can be moved sideways. They should be pushed together in the middle when the knife and specimen are adjusted. In this way, the danger of injury from the knife can considerably be diminished.
  • Page 25 Microtome Cryostat HM 505 E 2-10-2 DISPOSABLE BLADE CARRIER EC Fig. 9 With the disposable blade carrier EC, all Turn the knob (fig. 9.5) to move the anti-roll commercially available low profile blades of plate (fig. 9.10) forwards and backwards. The 80 x 8 mm and a facet angle of approx.

  • Page 26: Error Code Indication

    Microtome Cryostat HM 505 E Fig. 10 2-11 ERROR CODE INDICATION The instrument has an error code indication to The error code E-02 is also shown on the left define faster and better possible malfunctions. display (fig. 10.1) and indicates that the battery for the stored data is empty.
  • Page 27 Microtome Cryostat HM 505 E To avoid that the wet microtome is cooled The error code E-06 is also shown on the right down and consequently the guides freeze display (fig. 10.2) in case the actual solid, the error code E-03 is shown on the temperature is warmer than -5°C.

  • Page 28: Standard Equipment

    Microtome Cryostat HM 505 E 2-12 ACCESSORIES 2-12-1 STANDARD EQUIPMENT The microtome cryostat HM 505 E is equipped with the following accessories: Cat. No. 2 specimen stage, 30 mm round 715110 1 specimen stage, 40 mm round 715120 6 Cover caps for freezing stations...

  • Page 29: Additional Equipment (Optional)

    Microtome Cryostat HM 505 E 2-12-2 ADDITIONAL EQUIPMENT (OPTIONAL) Cat. No. Heat extractor 524500 Specimen stages: ∅ 30 mm 715110 ∅ 40 mm 715120 50 x 50 mm 715170 55 x 55 mm 715180 60 x 55 mm 715190 70 x 55 mm...
  • Page 30 Microtome Cryostat HM 505 E PART 3 THEORY OF OPERATION OF THE CRYOSTAT COOLING OF CHAMBER AND DEFROSTING The chamber of the cryostat is cooled by a To carry out the defrosting process, let hot refrigeration machine. In the rear upper area...
  • Page 31 Microtome Cryostat HM 505 E MICROTOME WITH RETRACTION The microtome of this cryostat is a rustproof The sum of sections made can also be seen on rotary microtome. The cutting movement is the display. For this, trimming value and carried out either manually by turning the sectioning values are added up.
  • Page 32 Microtome Cryostat HM 505 E SPECIMEN COARSE FEED AUTOMATIC APPROACH AND TRIMMING STAGES SYSTEM After changing the specimen, moving the The automatic approach system is used for the knife or knife carrier, it is necessary to adjust fast and exact approach of the specimen the specimen to the knife edge again.
  • Page 33 Microtome Cryostat HM 505 E PART 4 WORKING WITH THE CRYOSTAT PREPARING THE FREEZING-ON THE MICROTOME CHAMBER SPECIMEN Before sectioning, the microtome chamber The specimen is frozen-on to the specimen should be at a stable temperature around the stage with clinging groves with a freezing desired cutting temperature.
  • Page 34 Microtome Cryostat HM 505 E TEMPERATURE LIST FOR CRYO-SECTIONING The optimal cutting temperature of a specimen depends on the respective characteristics of the tissue especially on the fat content. The following table won by experience recommends cutting temperatures for some typical applications: Range A -10 to -20°C...
  • Page 35 Microtome Cryostat HM 505 E ORIENTATION AND SECTIONING AND TAKING TRIMMING OF SPECIMEN OFF SECTIONS Clamp the specimen stage together with the For sectioning, pre-select the desired section frozen specimen into the specimen holder. thickness by means of the buttons on the Tighten the orienting lever (fig.
  • Page 36 Microtome Cryostat HM 505 E HOW TO AVOID MALFUNCTIONS To cut usable sections, the following points - Moreover, the specimen stage as well as are of utmost importance: the knife edge and the microtome base must have a certain conductivity.
  • Page 37 Microtome Cryostat HM 505 E PART 5 MAINTENANCE OF THE CRYOSTAT Turn out the socket head screw (fig. 11.1) SHUTTING-OFF FOR in the middle of the front part of the microtome base plate with the hex head CLEANING wrench 5 mm; then slightly lift the base Cleaning of the cryostat depends on how plate (fig.
  • Page 38 Microtome Cryostat HM 505 E - Insert accessory shelves, accessories and knife carrier into the instrument. - Connect the mains plug to the power outlet. Turn on the mains switch. - Close the sliding window. Fig. 11 NOTE! To maintain the cooling power of the instrument, clean the fins of the condenser at least once a year, if necessary more often.

  • Page 39: Changing The Fluorescent Lamp

    Microtome Cryostat HM 505 E CLEANING AND CARE OF THE MICROTOME Carefully clean and dry the dismounted NOTE! examination microtome. As there is condensated humidity adjustment of the microtome a routine inside the microtome dry it very carefully. A maintenance should be performed by a trained drying closet at temperatures up to +60°C can...
  • Page 40 Microtome Cryostat HM 505 E VACUTOME FOR CRYOSTAT HM 505 SERIES STRETCHING AND SUCTION DEVICE INSTRUCTION MANUAL MICROM International GmbH Robert- Bosch-Str. 49 D- 69190 Walldorf 386240 - English...
  • Page 41 Microtome Cryostat HM 505 E MICROM International GmbH Robert- Bosch-Str. 49 D- 69190 Walldorf 386240 - English...
  • Page 42 Validity This instruction manual will be supplied together with each instrument. Further copies can be ordered at the nearest MICROM sales office by giving the serial number of the cryostat, the number of this instruction manual and the date of issue.
  • Page 43 Microtome Cryostat HM 505 E INTENDED USE Dear customer, Before operating the instrument, please read these instructions carefully to familiarize yourself with its proper operation and functions. Only skilled or specially trained personnel must work with this instrument. Please observe the listed and marked safety messages as well as the regulations of your respective lab.
  • Page 44 Microtome Cryostat HM 505 E Table of contents Safety precautions Part 1 Introduction Description of the Vacutome Technical specifications Part 2 Operating instructions Initial turn-on Setting the vacuum for stretching sections and disposing of section waste Blade carriers 2-3-1 Magnetic disposable blade carrier MV...
  • Page 45 Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. MICROM International GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements.
  • Page 46 Microtome Cryostat HM 505 E HAZARD OF INFECTION Use appropriate safety and disinfection measures when working with infectious specimens. HAZARD OF MALFUNCTION To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic environment. This means, that transmitters such as mobile phones must not be operated in their close vicinity.
  • Page 47 Microtome Cryostat HM 505 E PART 1 INTRODUCTION DESCRIPTION OF THE VACUTOME The unit consists either of a special magnetic disposable blade carrier MV or of a disposable blade carrier EV both of which are equipped with a vacuum anti-roll hood for the stretching and/or suction process.

  • Page 48: Technical Specifications

    Microtome Cryostat HM 505 E TECHNICAL SPECIFICATIONS Max. specimen size: 28 mm Max. section thickness to be disposed of: 80 µm Max. section thickness to be stretched: 80 µm Min. section thickness to be stretched: 1 µm Cryostat blades for magnetic disposable blade carrier MV:...
  • Page 49 Further information on the initial turn-on of a cryostat can be obtained from the enclosed instruction manual Microtome- Cryostat HM 505 N or HM 505 E. To start working with the Vacutome, first mount the blade carrier into the dovetail...
  • Page 50 Microtome Cryostat HM 505 E SETTING THE VACUUM FOR STRETCHING SECTIONS AND DISPOSING OF SECTION WASTE Fig. 1 The suction power of the required vacuum upper edge of the specimen, i.e. that the can be selected on the operating knob (fig.
  • Page 51 With the magnetic disposable blade To move the blade to a new position, put carrier MV, MICROM blades of 60 x 19 on the blade slider (fig. 3.2) onto the blade mm and a facet angle of approx. 33° can be and shift it.
  • Page 52 Microtome Cryostat HM 505 E Fig. 3 The knurled screw (fig. 2.6) allows the The lever (fig. 2.7) is used to fix or change adjustment of the edge of the vacuum anti- the clearance angle adjustment. roll hood in relation to the blade edge.
  • Page 53 Microtome Cryostat HM 505 E 2-3-2 DISPOSABLE BLADE CARRIER EV Fig. 4 With the disposable blade carrier EV, all Turning the grip (fig. 4.5), the vacuum commercially available low profile blades anti-roll hood (fig. 4.4) can be turned of 80 x 8 mm and a facet angle of approx.
  • Page 54 Microtome Cryostat HM 505 E Fig. 5 Lift the vacuum anti-roll hood (fig. 5.4), During breaks or while working on the the flap (fig. 5.6) beneath falls back in specimen or the specimen holder, the knife place and the slot (fig. 5.7) gets closed.
  • Page 55 Microtome Cryostat HM 505 E ACCESSORIES 2-4-1 STANDARD EQUIPMENT The Vacutome for the microtome cryostat HM 505 series is supplied with the following accessories: Cat. No. 1 Blade carrier (type as ordered) 1 Reusable filter insert 468050 5 Disposable paper bags...

  • Page 56: Theory Of Operation

    Microtome Cryostat HM 505 E PART 3 THEORY OF OPERATION The Vacutome includes a special blade carrier with which a vacuum can be applied just below the blade edge for stretching sections or the disposal of section waste. For this, the blade carrier features a slot that can be opened and closed by means of the vacuum anti-roll hood through an inserted flap.
  • Page 57 Microtome Cryostat HM 505 E PART 4 WORKING WITH THE VACUTOME PREPARING THE ORIENTATION AND VACUTOME TRIMMING OF SPECIMENS First insert the blade carrier onto the With the clamping lever loose, you have dovetail guide as already described in part the possibility to adjust the specimen 2-1 and clamp it with the clamping lever.
  • Page 58 Microtome Cryostat HM 505 E SECTIONING AND TAKING OFF SECTIONS For sectioning with a cryostat HM 505 E, pre-select the desired section thickness on the corresponding buttons of the operating panel (see part 2-6, Instruction Manual Microtome Cryostat HM 505 E).
  • Page 59 (see part 4-2, blade and exactly adjust the vacuum anti- Instruction Manual Microtome Cryostat roll hood in relation to the blade edge. HM 505 N or HM 505 E). - Select the appropriate freezing com- pound. CAUTION! If the specimen was frozen with liquid nitrogen, the specimen must adapt itself to the cutting temperature.
  • Page 60 Microtome Cryostat HM 505 E PART 5 MAINTENANCE OF THE VACUTOME SHUTTING-OFF FOR CAUTION! Turn the handwheel handle CLEANING into the lower position, i.e. the specimen holder is also in the lower reversal point. Cleaning of the Vacutome depends on how...
  • Page 61 Microtome Cryostat HM 505 E CAUTION! The handwheel handle must be in the lower position when the microtome is engaged into the clutch. - Insert accessory shelves, accessories and blade carrier into the instrument. - Connect the filter housing first with the tube on the suction side and then via the connecting bow with the blade carrier.

  • Page 62: Changing And Cleaning Filters

    Microtome Cryostat HM 505 E CLEANING AND CARE 5 - 3 CHANGING AND OF THE CRYOSTAT WITH CLEANING FILTERS VACUTOME Carefully clean and dry the disconnected To change or clean the filters, unscrew the microtome. As there is condensated screw-type cap (fig. 7.4) from the housing humidity inside the microtome dry it very (fig.
  • Page 63 Microtome Cryostat HM 505 E Fig. 7 1 = Connecting piece 5 = Connecting bow 2 = Housing 6 = Re-usable filter 3 = Disposable filter bag 7 = Connecting piece 4 = Screw-type cap MICROM International GmbH Robert- Bosch-Str. 49...

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