Microm HM 325 Instruction Manual
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Microm HM 325 Instruction Manual

Rotary microtome
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Rotary Microtome HM 325
ROTARY MICROTOME
HM 325
INSTRUCTION MANUAL
MICROM International GmbH
Robert-Bosch-Str. 49
69190 Walldorf
387821 - English

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  • Page 1 Rotary Microtome HM 325 ROTARY MICROTOME HM 325 INSTRUCTION MANUAL MICROM International GmbH Robert-Bosch-Str. 49 69190 Walldorf 387821 - English...
  • Page 2 Rotary Microtome HM 325 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 387821 - English...

  • Page 3: Warranty

    This MICROM product is warranted against defects in material and workmanship for a period of 1 year. Parts which prove to be defective during the warranty period will be repaired or replaced free of charge by MICROM International GmbH. No other warranty is expressed or implied.
  • Page 4 MICROM serial number: ............... Please check the MICROM Ser. No. on the type plate, which is placed on the rear side of your instrument and enter this number here. This way, questions and service can be handled faster.
  • Page 5 Description Rotary Microtome HM 325 Accessories 2-2-1 Standard equipment 2-2-2 Additional equipment (optional) Technical data Rotary Microtome HM 325 PART 3 OPERATING INSTRUCTIONS Setting up the microtome Cutting movement and retraction Locking and unlocking the handwheel Section thickness setting Section counter...
  • Page 6 Rotary Microtome HM 325 Specimen clamping 3-9-1 Universal cassette clamp 3-9-2 Universal cassette clamp, adjustable 3-9-3 Standard specimen clamp 3-9-4 Insert for round specimens, V insert and V distance piece 3-9-5 Foil clamp 3-10 Knife carrier 3-10-1 Disposable blade carrier ER...
  • Page 7 Rotary Microtome HM 325 EC Certificate of Conformity Name and address of MICROM International GmbH the manufacturer: Robert-Bosch-Straße 49 D-69190 Walldorf Product designation: Rotary Microtome Type reference: HM 325 -2 Notification to Competent Authorities: These medical devices have been registered with the German authority as “Microtomes”...
  • Page 8 Rotary Microtome HM 325 Hans Heid Walldorf, 16 June 2005 Managing Director MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 387821 - English...
  • Page 9 Rotary Microtome HM 325 PART 1 IMPORTANT INFORMATION WARNING SIGNALS AND SYMBOLS The installation and routine use of the HM 325 is easy and safe if the instructions in this manual are being observed. Note: Special instructions regarding operation of the instrument.
  • Page 10 Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. MICROM International GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements.

  • Page 11: Waste Disposal

    Rotary Microtome HM 325 HAZARD OF INFECTION Use the appropriate safety and disinfection measures when working with infectious specimens. According to the rules and regulations concerning the handling of infectious/radioactive contaminated material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.

  • Page 12: Intended Use

    Rotary Microtome HM 325 INTENDED USE The Rotary Microtome HM 325 from MICROM International GmbH is a highly efficient instrument which can be used for paraffin sectioning in biology, medicine and industry. Only skilled or specially trained personnel must operated the microtome, i.e. clamping the specimen, trimming, sectioning and taking off the sections from the instrument.
  • Page 13 The HM 325 will cut sections in a range from 0,5 µm up to 60 µm. For the protection of knife and specimen, the instrument retracts the specimen at the end of the cut.
  • Page 14 Rotary Microtome HM 325 Total overview Fig. 1 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 387821 - English...

  • Page 15: Additional Equipment (Optional)

    Rotary Microtome HM 325 ACCESSORIES 2-2-1 STANDARD EQUIPMENT The microtome HM 325 is supplied with the following accessories: 1 Hex head wrench 6 mm 1 Cover 1 Para Gard, 100 ml 1 Instruction manual 2 Cover plates 2-2-2 ADDITIONAL EQUIPMENT (OPTIONAL) 2-2-2-1 KNIFE CARRIER Cat.

  • Page 16: Optical Accessories

    Rotary Microtome HM 325 2-2-2-3 OPTICAL ACCESSORIES Cat. no.: Large field magnifier, 220 V 760160 Large field magnifier, 120 V 760170 Stereomicroscope Stemi 2000 755210 Adapter for Stemi 2000 532090 KL 1500 with ring illumination 230 V, 50 - 60 Hz...

  • Page 17: Additional Accessories

    Rotary Microtome HM 325 2-2-2-5 ADDITIONAL ACCESSORIES Fast freezing unit K 34 100 V/50 - 60 Hz 115 V/60 Hz 230 V/50 Hz 240 V/50 Hz Section transfer system STS 100 V/50 - 60 Hz 115 V/60 Hz 230 V/50 Hz...
  • Page 18 Rotary Microtome HM 325 TECHNICAL SPECIFICATIONS HM 325 Microtome: Section thickness range ..........0,5 - 60 µm Resolution: ..........0,5 µm for 0,5 - 2 µm ................1 µm for 2 - 10 µm ............... 2 µm for 10 - 20 µm ...............

  • Page 19: Setting Up The Microtome

    Rotary Microtome HM 325 OPERATING INSTRUCTIONS SETTING UP THE MICROTOME Note: The kind of the used examination materials and all special conditions for their processing, pre-treatment and, if necessary, storage as well as instrument controls correct safe operation responsibility of the operator.
  • Page 20 Rotary Microtome HM 325 Note: Remove the section waste tray to move or carry the instrument. The section waste tray can be pulled out of its proper position. • Before starting section, instrument, knife carrier and section waste tray should be treated with a commercially available paraffin repellent.

  • Page 21: Cutting Movement And Retraction

    Rotary Microtome HM 325 CUTTING MOVEMENT AND RETRACTION The cutting movement of the microtome is generated by turning the handwheel. • To start the cutting movement, turn the handwheel. • As the specimen moves down, sectioning is carried out (cutting movement, fig. 6.2).

  • Page 22: Section Thickness Setting

    Rotary Microtome HM 325 SECTION THICKNESS SETTING • The selected section thickness is set on the turning knob (fig. 5.1) on the right or left side. • The setting is indexed. Intermediate values can also be set. • The selected value is readable on the window (fig.
  • Page 23 Rotary Microtome HM 325 SPECIMEN FEED 3-6-1 COARSE FEED For the fast forward and backward travel between specimen and knife edge, the microtome has a coarse feed system. • By means of the coarse feed wheel (fig. 6.1) specimen holder moved horizontally backwards and forwards.

  • Page 24: Trimming And First Cuts

    Rotary Microtome HM 325 3-6-2 TRIMMING AND FIRST CUTS The feed can also be carried out in graduated trimming stages. Trimming stages of 10 µm and 30 µm are available. • For trimming stage 1 (10 µm), turn the trimming lever (fig. 7.1) until a first resistance can be felt and turn the handwheel at the same time.
  • Page 25 Rotary Microtome HM 325 ACOUSTIC SIGNAL FOR FRONT END POSITION OF THE SPECIMEN MOVEMENT • About 1 mm before the front end position of the specimen feed an acoustic signal sounds during each rotation of the handwheel! Warning: When the signal sounds, a feed range of no more than 1000 µm is available...

  • Page 26: Specimen Orientation

    Rotary Microtome HM 325 ADAPTERS FOR SPECIMEN CLAMPING 3-8-1 ADAPTER, NON-ORIENTING This adapter serves non-orienting fastening of the specimen clamps directly on the cylinder of the instrument. 3-8-2 SPECIMEN ORIENTATION • Using the specimen orientation to fasten the specimen clamps on the cylinder head. This allows the specimen to be aligned in relation to the knife.
  • Page 27 Rotary Microtome HM 325 3-8-3 CHANGING AND/OR CLAMPING SPECIMEN CLAMPS The available specimen clamps are all clamped or removed in the same way. • To change the specimen clamping system, press clamping lever (fig. 11.1) downwards and pull it sideways.

  • Page 28: Readjusting Specimen Clamps

    Rotary Microtome HM 325 3-8-4 READJUSTING SPECIMEN CLAMPS Frequent use of the clamping lever (fig. 12.1) can lead to the fact that the specimen clamps cannot be clamped optimally anymore. If the necessary readjustments are not carried out, it might even be possible that the specimen clamp cannot be clamped.

  • Page 29: Specimen Clamping

    Rotary Microtome HM 325 SPECIMEN CLAMPING To clamp specimens, different specimen clamping systems are available. With the orienting adapter it is simple to align the specimen properly in relation to the knife. 3-9-1 UNIVERSAL CASSETE CLAMP The universal cassette clamp (fig. 14) represents a quick change system.

  • Page 30: Standard Specimen Clamp

    Rotary Microtome HM 325 3-9-2 STANDARD SPECIMEN CLAMP The standard specimen clamp (fig. 16) is used for rectangular and square paraffin and plastic blocks. • Insert the specimen against the fixed jaw (fig. 16.2) first. • Then turn the knob (fig. 16.3) to tighten jaw (fig.
  • Page 31 Rotary Microtome HM 325 3-9-3 INSERT FOR ROUND SPECIMEN, V-INSERT AND V-DISTANCE PIECE To cut round specimens, the insert for round specimens (fig. 17.A) with defined diameters of 6, 15 and 25 mm (special sizes on request) or the V- insert (fig.

  • Page 32: Foil Clamp

    However, it is possible to turn in Z-axis 60° in either direction. • Then insert the foil clamp. • According to the various specimens, it might be helpful to use in addition MICROM's sandwich supporting material (cat. 176010) on the right and left side between specimen and clamping jaw.

  • Page 33: Disposable Blade Carrier Er

    Rotary Microtome HM 325 3-10 KNIFE CARRIERS Hazard of hand injuries: Due to moving parts in connection also with the microtome knife, a danger area arises, which might lead to hand injuries in case of non-compliance with the safety features of the microtome and when disregarding the instruction manual.
  • Page 34 Rotary Microtome HM 325 Using high profile blades: When using high profile blades, first remove the spacer strip (fig. 19.3). • For this, turn the clamping lever (fig. 19.7) to the front until the stop. • The press the stop pin into the knife carrier and further turn the clamping lever (fig.

  • Page 35: Disposable Blade Carrier E

    Rotary Microtome HM 325 3-10-2 DISPOSABLE BLADE CARRIER E Inserting the blade: • The disposable blade carrier E is designed to take all commercially available high and low profile blades. • Insert the blade 20.5). • When using high profile blades, first loosen the two screws (fig.
  • Page 36 Rotary Microtome HM 325 Note: By experience, usable cuts are only achieved at a clearance angle of 10° or more. • If the clamping lever (fig. 20.3) is loosened, the upper part of the knife carrier can be moved 1 cm to the left or right side.

  • Page 37: Knife Carrier C

    Rotary Microtome HM 325 3-10-3 KNIFE CARRIER C Inserting the knife: • To insert the knife, the clamping screws (fig. 21.3) must be unscrewed slightly so the knife can be pushed in from the side. • The height of the knife is adjusted with the two knurled nuts (fig.
  • Page 38 Rotary Microtome HM 325 Moving the knife carrier on the console: • Loosen the clamping lever (fig. 21.5) on the left side of the knife carrier to move the carrier forwards and backwards on the dovetail guide. • This allows a rough adjustment of knife and specimen.
  • Page 39 Rotary Microtome HM 325 3-10-4 READJUSTING KNIFE CARRIERS Frequent use of the clamping levers can lead to the fact that the knife carriers cannot be clamped optimally anymore. If the necessary readjustments are not carried out, it might even be possible that the knife carriers cannot be clamped anymore.

  • Page 40: Section Waste Tray With Integrated Arm Rest

    Rotary Microtome HM 325 3-11 SECTION WASTE TRAY WITH INTEGRATED ARM REST The section waste tray with integrated arm rest can easily be cleaned. • The section waste tray surrounds the knife carrier area and allows non-tiring working with the microtome.

  • Page 41: Large Field Magnifier

    Rotary Microtome HM 325 3-12 LARGE FIELD MAGNIFIER The large field magnifier (fig. 26.1) is for looking at section production. • The light is connected to the power outlet with the power cord (fig. 26.2) and turned on and off with the switch (fig. 26.3).

  • Page 42: Part 4 Working With The Microtome

    Rotary Microtome HM 325 PART 4 WORKING WITH THE MICROTOME Hazard of hand injuries: Due to moving parts in connection also with the microtome knife, a danger area arises, which might lead to hand injuries in case of non-compliance with the safety features of the microtome and when disregarding the instruction manual.
  • Page 43 As the facet has a divergence from the knife main surface of approx. 5°, the difference is approx. 5°. Set the correct angle on MICROM instruments at 10°. Fig. 30 MICROM International GmbH Robert-Bosch-Str.
  • Page 44 Rotary Microtome HM 325 HOW TO AVOID MALFUNCTIONS Specimen preparation: • When preparing specimens, be sure that a suitable embedding medium, fixation, dehydration and infiltration time are chosen. Specimen temperature: • Sectioning carried ambient temperature (excluding frozen sections). • If the temperature is too high, the paraffin softens.

  • Page 45: Possible Sources Of Errors - Cause And Removal

    Rotary Microtome HM 325 POSSIBLE SOURCES OF ERRORS – CAUSE AND REMOVAL Problem Cause Removal − − Thick-thin-sections Blunt knife/blade. Move knife and/or insert a new one. − − Knife angle, therefore, clearance angle unfavorable clearance angle. adjustments, until an optimal angle can be found.

  • Page 46: Maintenance And Care

    Rotary Microtome HM 325 PART 5 MAINTENANCE AND CARE CLEANING AND CARE Cleaning and care of the microtome should be carried out daily. • Lock the handwheel. • Remove the knife from the knife carrier. Clean it and store it in a knife case! •...

  • Page 47: Maintenance

    For more information, please contact the nearest MICROM sales office. Note: We strongly recommend to not carry out repair by yourself. All warranties and guarantees would then be null and void! Repair work must only be carried out by an authorized service technician.

  • Page 48: Returning The Instrument For Repair Or Routine Maintenance

    Repair or maintenance work are normally carried out at the site of installation. If this is not possible for some specific reasons, the instrument can be returned to MICROM. The contact address can be found at the beginning of this instruction manual. •...

  • Page 49: Disposal Of The Instrument After Final Shutdown

    Rotary Microtome HM 325 For transportation outside closed buildings, please observe the following measures: • Lock the handwheel. • Remove knife or blade and store it in a safe way. • Remove section waste tray. • To lift the instrument, use the two recessed grip on the lower side of the instrument.

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