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ESPECTROFOTÓMETRO 4201/20 4201/20 SPECTROPHOTOMETER SPECTROPHOTOMÈTRE 4201/20 REF. / CODE / RÉF. HJB003 Este manual es parte inseparable del aparato por lo que debe estar disponible a todos los usuarios del equipo. Le recomendamos leer atentamente el presente manual y seguir rigurosamente los procedimientos de uso para obtener las máximas prestaciones y una...
ENGLISH LANGUAGE INDEX 1-21 Spanish 22-41 English 42-61 French TABLE OF CONTENTS 1 General information ......................24 1.1 Safety information ....................24 1.1.1 Danger and warning information ..............24 1.1.2 Prevention label .................... 24 1.2 Product information ....................25 1.2.1 Product appearance ..................25 1.2.2 Product description ..................
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ENGLISH 5 General operation ........................30 5.1 Getting started ......................30 5.1.1 Use the knob ....................31 5.1.2 Use the keyboard ...................31 5.1.3 Main interface ....................31 5.1.4 Common operations ..................32 6 Measurement ........................32 6.1 Important guidelines ....................32 6.2 Prepare and check ....................33 6.3 Absorbance measurement ..................33 6.4 Transmission measurement ..................34 6.5 The calibration standard sample measures the unknown sample concentration....35 6.6 Enter the equation coefficient to measure the concentration ..........36...
ENGLISH 1 GENERAL INFORMATION 1.1 Safety information Unpack and read this entire manual before installing or operating this equipment. Pay attention to all danger and warning instructions. Failure to do so may result in serious personal injury to the operator or damage to the equipment. To ensure that the protection provided by this equipment is not impaired, do not use or install this equipment in any way other than as specified in this manual.
ENGLISH 1.2 Product information 1.2.1 Product appearance 1 – LCD display 2 – Knob 3 – Membrane keyboard 4 – Sample room 5 – Pull rod Fig. 1-1 Appearance 1.2.2 Product description This model of spectrophotometer can be used for laboratory and field analysis. The instrument has basic photometric and concentration measurements.
ENGLISH 3 INSTALLATION 3.1 Unpack the instrument This model of spectrophotometer contains the following parts: • Spectrophotometer 1 PC • Instruction Manual 1 PC • 10 mm glass cuvette 4 PCS • 10 mm quartz cuvette 2 PCS (UV-1150 only) •...
ENGLISH 3.3 Connection 3.3.1 Power connection Check the instrument power switch to ensure that in the closed position, as shown in Figure 3-2, insert the female end of the power cord into the instrument’s power connector and the male end into the power outlet.
ENGLISH 3.3.3 Computer connection As shown in Figure 3-5, plug one end of the USB communication cable into the instrument’s USB port (Type B, square) and the other end into the computer’s USB port (Type A, flat). Note: The USB communication cable is an accessory of the computer application software (Optional configuration, need to be purchased separately) and is not configured with the instru- ment.
ENGLISH Fig. 4-1 Warm up Fig. 4-2 System initialization and calibration 5 GENERAL OPERATION 5.1 Getting started Fig. 5-1 Operation panel Page 30 Version 6, July 2024 User’s manual for visible spectrophotometer HJB003...
ENGLISH 5.1.1 Use the knob The knob of the instrument has two operation modes. • Rotating Rotate the knob to switch test mode in the main interface. Rotate the knob to change the parameter value in the parameter setting interface. •...
ENGLISH Absorbance measurement Measure the absorbance value of the sample. Transmissivity measurement Measure the transmittance of the sample. The calibration standard sample measures the unknown sample concentra- tion By measuring the absorbance value of a standard sample and entering the corresponding concentration, a standard curve is established with the origin, and the concentration of the unknown sample is measured using the standard curve.
ENGLISH • During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfec- tant) regarding the handling of sample material. • Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant local laboratory regulations.
ENGLISH 6.4 Transmission measurement • On the main screen, turn the knob to the “Transmission” on the screen and press “ENTER” to enter (Figure 6-3). Fig. 6-3 Transmissivity measurement 1. Press the knob to pop up the setting interface (Figure 6-4), rotate the knob to change the measu- rement wavelength, press “ENTER”...
ENGLISH 6.5 The calibration standard sample measures the unknown sample concentration • On the main screen, turn the knob to the “Concentration” and press “ENTER” to enter (Figure 6-5). Fig. 6-5 The calibration standard sample measures the unknown sample concen- tration 1 Press the knob to pop up the setting interface (Figure 6-6), rotate the knob to change the measu- rement wavelength, press “ENTER”...
ENGLISH Fig. 6-8 Measuring standard sample 4. Place the “reference” in the measurement channel, close the sample room, and press “ZERO” to do blank. 5. Place the “sample” in the measurement channel, close the sample room, and wait for the measured value to read the result stably.
ENGLISH 2. According to the prompt (Figure 6-11), enter the slope K and intercept B values of the equation C=K*A+B, and press “ENTER”to finish; Fig. 6-11 Input slope and intercept 3. Place the “reference” in the measurement channel, close the sample room, and press “ENTER” to do blank.
ENGLISH 1. Press the knob to pop up the setting interface (Figure 6-13), rotate the knob to change the mea- surement wavelength, press “ENTER” to confirm; Fig. 6-13 Set the measurement wavelength 2. Rotate the knob to set the gain of the amplifier (1, 2, 4 or 8 times); 3.
ENGLISH 7.2 Troubleshooting Problem Cause Solution 1 The power supply to the instrument is 1 Turn on the power supply Power on, not turned on 2 Improve connection no response 2 Power cord connection is not reliable 3 Replace fuse 3 Fuse destroyed Boot, light source positio-...
ENGLISH 7.3 Spare Parts Replacement 7.3.1Fuse replacement Danger! Be sure to turn off the power of the instrument and unplug the power cable before replacement! 1. Prepare a 3×75 flat blade screwdriver; 2. Turn off the device and unplug the power cord; 3.
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ENGLISH Fig. 7-3 Instrument case fixing screws 4. Unscrew the fixing screws of the lamp housing cover (Figure 7-4) and remove the lamp housing cover; Fig. 7-4 Open the lamp housing cover 5. Replace the tungsten lamp (Figure 7-5). Wear cotton gloves. Remove the old tungsten lamp, and insert the new tungsten lamp into the tungsten lamp holder;...
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