Tips And Tricks - Cutting Artifacts - Slee CRYOSTAT MEV Operating Manual

9.15 Tips and tricks – cutting artifacts
Cutting artifact
Alternating thin and thick
cuts
Squeaking sound when
cutting, cuts show chatter-
ing
Formation of fractures in
frozen tissue
Feed takes place but no
sections emerge
Sections distort or roll up
Section cracks up
Cuts thaw during cutting
Frost on the blade
Section sticks to the anti-
roll blade
Section twists to one side
Section detaches from slide
Manual_MEV_MEV+_2024-06-001.00_EN.docx
Cause
wrong temperature of the cutting edge /
blade
wrong temperature of the cryo chamber
sample is not optimally fixed on specimen
holder
sample is not optimally fixed on specimen
holder, vibrates during cutting
freezing was too fast
sample is too big
loose blade
sample is not optimally fixed on specimen
holder
wrong blade angle
anti-roll plate is too far in the front
specimen is not frozen yet
gap of the anti-roll plate is too small
thickness of sections is too thin
blunt blade
sample is over frozen
destroyed or dirty blade / edge
cryostat or cutting temperature is too high
cryostat was open too long
gap of the anti-roll plate is too small
tissue or fat covers the anti-roll plate
inappropriate cryostat or blade tempera-
ture
deposits on cutting edge
gash in the blade, blunt blade
anti-roll plate is broken
cut fixed tissue without adhesive
no adhesive used
fat tissue
cartilaginous tissue
Remediation
select temperature according to the tissue
type
adjust temperature
remove sample and freeze again
remove sample and freeze again
freeze new sample, if possible
check clamping
remove sample and freeze again
correct angle
adjust anti-roll plate with the adjusting
screw
let tissue freeze longer
set up / adjust
increase thickness of sections
change blade and / or move laterally
freeze new sample, if possible
change anti-roll blade
correct temperature
close the cryo chamber
set up / adjust
clean anti-roll plate
correct temperature
clean blade / cutting edge
change blade and / or move laterally
change anti-roll blade
use adhesive
37