Slee Cryostat MEV Operating Instructions Manual
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Slee Cryostat MEV Operating Instructions Manual

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OPERATING
INSTRUCTIONS
INSTRUCTIONS
Cryostat
MEV
INS2000GB
2011-12-11
Instructions – MEV
1

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Summary of Contents for Slee Cryostat MEV

  • Page 1 OPERATING INSTRUCTIONS INSTRUCTIONS Cryostat INS2000GB 2011-12-11 Instructions – MEV...
  • Page 2 Instructions – MEV...

  • Page 3: Table Of Contents

    CONTENTS 1. INTENDED USE ............4 2. SYMBOLS ............4 3. SAFETY NOTES ............4 4. COMPONENTS ............8 5. SPECIFICATIONS ............9 6. UNPACKING AND INSTALLATION ............10 7. INITIAL OPERATION ............13 8. OPERATION OF MICROTOME ............19 9.

  • Page 4: Intended Use

    1. INTENDED USE The floor standing open top cryostat MEV is intended for rapid freezing and cutting thin sections of frozen specimens for professional use in routine and research laboratories in the fields of biology, medicine and industry. The system of knife advance operates very reliable from 0.5 to 100 µm with trimming function up to 300 µm.
  • Page 5 3.1 Hand wheel stop Always use the finger protection with the knife-/blade holder and put hand wheel in stop position • before working with the knife or specimen • before changing the specimen • during break time The hand wheel can be stopped in any position.
  • Page 6 3.2 FINGER PROTECTION Use the finger protection always • Before you start any work with the knife or specimen • Before changing the specimen • During work break Finger protection released. Finger protection activated. Instructions – MEV...
  • Page 7 3.3 ELECTRICAL POWER CONNECTION Do not use any extension lead. Make sure that electric power is constant: • This should be examined during installation of the unit by a competent person • Use a dedicated fuse for the unit • Do not connect another unit to the same power circuit. •...

  • Page 8: Components

    4. COMPONENTS The SLEE open top cryostat MEV is provided with the following standard components: • • • • Basic unit with refrigeration system • • • • Semi-automatic rotary microtome • • • • Operation manual 2x Specimen holder •...

  • Page 9: Specifications

    5. SPECIFICATIONS Operating temperature range +10 °C to +25 °C 0.5 - 100 µm Section thickness setting range Section thickness selection 0 - 2 µm in 0.5-µm steps 2 - 20 µm in 1-µm steps 20 - 50 µm in 2-µm steps 50 –...

  • Page 10: Unpacking And Installation

    6. UNPACKING AND INSTALLATION 6.1 UNPACKING THE INSTRUMENT In standard design you will receive the unit in a wooden box standing on a wooden pallet. It is equipped with four rollers and two feet, adjustable in height in the front. Remove the screws from the wooden box with the head screws screwdriver or battery screwdriver with insert for head screws.
  • Page 11 6.2 INSTALLATION SITE REQUIREMENTS The site for installation should meet the following requirements: • A maximum ambient temperature of 25 °C • No direct sunlight into the cooling chamber • A minimum distance between wall and rear of the instrument of 10-20 cm should be guaranteed •...
  • Page 12 6.3 COLLAPSIBLE HANDWHEEL LEVER The instrument is delivered with a ready to use hand wheel lever that is folded away for transport reasons. • Before you start any work unfold the hand wheel lever by simply pulling the lever into a horizontal position. •...

  • Page 13: Initial Operation

    7. INITIAL OPERATION 7.1 MAIN DISPLAY The main display will show the day and time, the TUE 13:05:05 cutting thickness, the trimming thickness, the current CUT: 7 m Trim: 19 m chamber temperature, the target chamber temperature, Cha: -20 °C (-22) QF: 0 the status of the quick-freeze unit (optional) and the Status:OK...
  • Page 14 7.3 SOFTWARE SETTINGS > Chamber temp -20 °C Press to open the Settings menu. Quickfreeze UV disinfection Light Auto To move further down in the main menu list, Retraction 5 µm Start defrost press Contrast > TIME TUE 13:05:05 To move further up in the main menu list, >...
  • Page 15 7.4 AUTOMATIC DEFROST, DISINFECTION AND POWER SAVING SETTINGS > Chamber temp -20 °C Press to open the Settings menu. Quickfreeze UV disinfection Light Auto TIME 13:05:05 Select Program settings and press to open the Language English menu for setting automatic defrost and power saving Display version times.
  • Page 16 Disinf. delay 12___ Select temperature increase days and set the days at Incr. start 21:00 that the automatic power saving mode will be active Incr. end 04:00 for 24 hours. > Incr. days ____56_ (e.g. Fridays and Saturdays in this example) 1-Monday 2-Tuesday 3-Wednesday 4-Thursday 5-Friday 6-Saturday 7-Sunday Settings for disinfection parameters: >...
  • Page 17 7.5 List of available software settings Chamber temperature -35 °C to ambient The recommended working temperature is -15 °C to -25 °C Object cooling (optional) ON / OFF If the instrument is equipped with the optional object cooling system, its function can be activated or deactivated.
  • Page 18 Chamber Increase For power saving, a higher temperature can be set for defined times and/or days. The selected temperature should be below 0 °C to avoid damage to the microtome by freezing moisture. 0 - 200 µm Retraction Start defrost Activate A manual defrost procedure is activated.

  • Page 19: Operation Of Microtome

    8. OPERATION OF MICROTOME 8.1 INSERTION AND ORIENTATION OF KNIFE Activate hand wheel lock. Loosen the knife fixation by turning two screws in the front of the knife holder counter clockwise Remove/Insert knife Adjust knife height by turning the wheels on the left and right side of the knife holder base.
  • Page 20 8.2 INSERTION AND ORIENTATION OF DISPOSABLE BLADE Activate hand wheel lock. Remove finger protection. Loosen the blade fixation by turning the right lever counter clockwise. Remove/Insert blade from one side. Tighten the blade fixation by turning the right lever clockwise. Loosening of blade fixation and insertion of new blade.
  • Page 21 For adjusting the cutting angle of the blade, loosen the blade holder fixation by turning the right lever on the blade holder base counter clockwise. Set new angle for cutting by turning the knob in the front of the blade holder or by manual tilting the blade holder.
  • Page 22 8.3 SPECIMEN MOUNTING The instrument is equipped with 24 freezing positions. The temperature is approx. 10 °C lower than the actual cryochamber temperature. In order to prepare a specimen for sectioning, place the specimen holder on a freeze point, add some drops of embedding medium (e.g.GSV1) on the holder and carefully press the object on top.
  • Page 23 8.5 SPECIMEN ORIENTATION ADJUSTMENT Turn hand wheel to its highest position and activate the hand wheel stop. For orientation of the specimen, open the fixing lever. For upward and downward orientation use the upper orientation wheel. For orientation to the left and right use the left orientation wheel.
  • Page 24 8.6 FAST APPROACH TO SPECIMEN Press to move the knife holder base towards the sample. Press to move the knife holder away from the sample. 8.7 SLOW APPROACH TO SPECIMEN Press to move the knife holder base slowly towards the sample. 8.8 THICKNESS ADJUSTMENT FOR TRIMMING To change the trimming settings, press continuously and press...
  • Page 25 8.11 MANUAL CUTTING Deactivate the hand wheel stop. Release the finger protection. Turn hand wheel evenly clockwise. A counter clockwise movement will also result in cutting and incremental advance of the specimen. Approach specimen either by trimming or slow approach. TUE 13:05:05 Set the required section thickness.
  • Page 26 8.12 ANTI-ROLL PLATE Each instrument is equipped with an anti-roll plate. To adjust the height of the anti-roll plate turn the wheel to the left or right. For pick up of the samples turn the anti-roll plate side wards with the help of the knob.
  • Page 27 8.13 TIPS AND TRICKS It is common knowledge that the temperature of the cryostat plays an important role in the quality of the tissue sections obtained. Each type of tissue needs a different temperature as shown in the following list. The temperature ranges indicated in the table below are approximate values and may require adjustments for individual tissues.

  • Page 28: Operation Of Cryostat

    9.2 QUICK FREEZE POSITION [only applicable to instruments equipped with this feature] For convenience of the user, the cryostat MEV can be equipped with a quick freeze shelf with two positions located in the object freezing shelf. Upon activation the installed peltier element will cool down the two quick freeze position to approximately -55 °C.
  • Page 29 9.3 HEAT EXTRACTOR [only applicable to instruments equipped with this feature] For a more rapid freezing process with improved structural preservation of tissues and a flattened block surface, the instrument can optionally be equipped with an heat extractor. Apply the heat extractor on top of the specimen during the freezing process.
  • Page 30 9.4 AUTOMATIC/MANUAL DEFROST For optimum performance of the cooling unit a low thermal contact resistance at the cooling lamella is required. Upon frequent use of the instrument moisture will condensate and freeze on the surface of the cooling lamella and increase the thermal contact resistance.
  • Page 31 9.5 COMPLETE DEFROST OF THE INSTRUMENT It is highly recommended to completely defrost the instrument at least every 6 months. If the instrument is used regularly in warm tropical climate a more frequent complete defrost might be necessary. Turn hand wheel to its highest position and activate the hand wheel stop.

  • Page 32: Cleaning And Maintenance

    10. CLEANING AND MAINTENANCE 10.1 CLEANING The recommended frequency of cleaning of the cryostat chamber depends on how frequently the instrument is used. Wear protective clothing and disposable gloves according to Good Laboratory Practices. Please take note of the safety aspects of the instrument. Do not use acetone or xylene for cleaning the unit.
  • Page 33 Check of temperature sensor in cryochamber. Monthly Cleaning of cryostat ventilation (see section 10.4). Yearly Complete Service (performed by authorized SLEE service technician) • check of all functions • complete defrost • removing of microtome • oiling of movable parts •...

  • Page 34: Service

    11. SERVICE Internal components should only be serviced by technicians authorized by SLEE. If technical service or spare parts are necessary, please contact your local SLEE Medical distributor. Please have the following information available: • Complete contact details • Type of instrument and serial number •...

  • Page 35: Optional Accessories

    12. OPTIONAL ACCESSORIES Standard Knife Holder #10156010 Disposable Blade Holder (low profile) #10157005 Disposable Blade Holder (high profile) #10158005 2x Quick freezing positions # 10159000 UV disinfection # 10169000 Cutting counter #10090020 Heat extractor #33000316 Object holder (Ø 22 mm) #30000002 Object holder (Ø...

  • Page 36: Warranty

    13. WARRANTY SLEE Medical GmbH guarantees that the product delivered has been subjected to a comprehensive quality control procedure, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. SLEE Medical GmbH guarantees that the instrument is manufactured under an ISO 9001 quality management system.

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