1. Manuals
  2. Brands
  3. Tianlong Manuals
  4. Laboratory Equipment
  5. Gentier X3E
  6. User manual

Tianlong Gentier X3E User Manual

Real-time pcr system
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Real-time PCR System
Gentier X3E/X3R/X3S/X3C
USER MANUAL

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Gentier X3E and is the answer not in the manual?

Questions and answers

Related Manuals for Tianlong Gentier X3E

Summary of Contents for Tianlong Gentier X3E

  • Page 1 Real-time PCR System Gentier X3E/X3R/X3S/X3C USER MANUAL...
  • Page 2 Real-time PCR System Real-time PCR System User Manual Created by Xi’an Tianlong Science and Technology Co. Ltd.
  • Page 3 Real-time PCR System Disclaimer and Copyright Copyright © 2023-2024 Xi’an Tianlong Science and Technology Co., Ltd. (hereinafter referred to as XATL Co., Ltd.). All rights reserved. All contents of this manual (including but not limited to text, trademark, logo, buttons icons, graphics, tables, data, etc.) are subject to the copyright and other intellectual property rights of...
  • Page 4 Real-time PCR System is a registered trademark of XI’AN TIANLONG SCIENCE AND TECHNOLOGY CO., LTD. Intended Use The Gentier real-time PCR system (hereinafter referred to as Gentier instrument) is based on polymerase chain reaction (PCR) principle to compound and amplify the specific gene with the matched detection reagent.
  • Page 5 Real-time PCR System Safety Labels on Transport Package Label Description Fragile: The items inside are fragile, please handle with care. This Side Up: It indicates the upward side of the transport package. Keep Dry: Keep the transport package away from rain or any liquid. IVD Instrument: The product belongs to In Vitro Diagnostic equipment.
  • Page 6 Real-time PCR System disinfect this area, keep necessary precautions during operation and take corresponding protective measures at the same time. High Temp.: It indicates that a certain area of Gentier instrument may produce high temperature. Remind users to carefully operate the instrument and caution against burns.
  • Page 7 Real-time PCR System Symbols Used in This Manual Symbol Description Caution: Remind user to pay attention to a certain operation. Operating Gentier instrument in any manner unspecified in this manual may result in instrument damage or abnormal function. Reminding: Provide important information regarding the operation and successful application of Gentier instrument, including the information explained in further detail elsewhere in this manual.
  • Page 8 Real-time PCR System Safety and Precautions The operation, maintenance and repair of Gentier instrument shall strictly follow the safety specifications listed in this section and through this manual. The design of Gentier instrument has fully considered its biological contamination protection, electrical safety protection and mechanical motion protection.
  • Page 9 Real-time PCR System Prohibit: Never move Gentier instrument while it is running. Caution: For protection against overheating hazards, the openings on the instrument are designed for ventilation. Please do not block these openings or cover the instrument with dust cover and other materials while it is running. Caution: The installation and transportation of Gentier instrument should be performed by professional engineer or under professional guidelines, or if any damage to instrument occurs, XATL Co., Ltd.
  • Page 10 Real-time PCR System III. Electrical Safety and Precautions Prohibit: The voltage of Gentier instrument can cause harms to human body. Please cut off the power supply before opening the instrument shell, and it is prohibited to replace any part of the instrument while it is electrified. Caution: Gentier instrument should be properly grounded, any damage of the internal or external grounding path may cause danger.
  • Page 11 Real-time PCR System IV. Environmental Safety and Precautions Caution: Gentier instrument is for indoor use. The room should be well ventilated and without corrosive gas. Prohibit: Never run Gentier instrument in places that have or may have flammable and explosive gas. Reminding: The working environment temperature of Gentier instrument should be 10°...
  • Page 12 Real-time PCR System VI. Electromagnetic Compatibility (EMC) Standard The electromagnetic compatibility of Gentier Instrument is classified into Class A and Group I. It shall be installed and used in accordance with the electromagnetic compatibility information in this section. 1) Gentier Instrument meets the emission and disturbance immunity requirements stipulated in IEC 61326-1 and IEC 61326-2-6.

  • Page 13: Table Of Contents

    Real-time PCR System Content A. OVERVIEW ............................. 1 1. A ..............................1 PPLICATION COPE 2. I ......................1 NSTRUMENT OMPONENT AND TRUCTURE 3. P ............................3 RODUCT NTRODUCTION 3.1 Instrument Parameters and Characteristics....................3 3.1.1 General Parameters of Instrument ....................3 3.1.2 Instrument Technical Parameters .....................4 3.1.3 Instrument Characteristics ........................6 3.2 Software Characteristics ............................
  • Page 14 Real-time PCR System 2.1 Menu Bar ..................................22 2.2 Tool Bar ..................................56 2.3 Home Page .................................. 56 2.3.1 Experiment Tab ............................57 2.3.2 Analysis Tab ............................. 61 2.3.3 Instrument Tab............................62 2.3.4 Item Tab ..............................64 2.3.5 Current Login User and Switch User ..................... 65 2.4 Operation Area .................................
  • Page 15 Real-time PCR System F. INSTRUMENT CLEANING AND MAINTENANCE ............. 161 1. I ..................161 NSTRUMENT LEANING PERATION NSTRUCTIONS 1.1 Clean Instrument Shell ............................161 1.2 Clean Touch Screen .............................. 162 1.3 Clean Loading Platform and Sample Block ....................162 2. I ................
  • Page 16 Real-time PCR System User Manual Modification Points between Versions Modification Point Current Version Page Date Manual Version; Modification Time Safety Labels IV/V User Manual Modification Points This page between Versions V1.1 10.01.2024 Software Run Environment Reminding Contact Information Note: This modification points are only for client reference. If you need modification details, please contact XATL Co., Ltd after-sale service referring to “G.

  • Page 17: Overview

    All instruments share the same appearance of sides and back while only the front and top are differentiated. Gentier X3E and Gentier X3R share the same appearance, as shown in figure A-1. Gentier X3S and Gentier X3C share the same appearance, as shown in figure A-2.
  • Page 18 Real-time PCR System Figure A-1. Gentier X3E and Gentier X3R Structure...

  • Page 19: Product Introduction

    Real-time PCR System Figure A-2. Gentier X3S and Gentier X3C Structure 1.Cabin Indicator Light 2. Sample Cabin 3. Instrument Switch 4. Detachable Cover 5. Air Vent 6. USB Port 7. Speaker 8. Tablet Cover 9. Sample Block 10. Tablet 11. Network Port 12.

  • Page 20: Instrument Technical Parameters

    Real-time PCR System Altitude: Below 4000m; Atmospheric Pressure: 56.0kPa~106.0kPa; Storage and Transportation Environment: ► Temperature: -20° C~55° C, with transport package; Relative humidity: 0%~93%, non-condensing; Atmosphere Pressure: 26.4kPa~106.0kPa; Running Noise: ► The maximum running noise shall not exceed 65 decibels. 3.1.2 Instrument Technical Parameters Thermology Parameters ►...
  • Page 21 ► Repeatability: CV ≤ 1%. ► Linear Correlation: │r│≥ 0.995, with no less than five magnitudes concentration gradients. Human-computer Interaction System Parameters ► Touch Screen: Gentier X3E and Gentier X3R are equipped with a tablet with 13.3-inch screen which is standalone. Prompt...

  • Page 22: Instrument Characteristics

    Real-time PCR System  RAM: 4GB or higher;  Storage Device: 256 GB or higher Caution: The main control computer of Gentier instrument is not designed for online use so connect it to internet may cause risks of computer virus or hacker attack. XATL Co., Ltd. is not be responsible for any damages caused by that risks.

  • Page 23: Software Characteristics

    Real-time PCR System 3.2 Software Characteristics ► Software Interface: Wizard-style interface, intuitive layout and program setting, easy for operation. ► Multiple Functions: The software possesses multiple analysis functions that can adapt to a variety of experimental requirements, such as absolute quantification analysis, relative quantification analysis, melting curve, high resolution melting (HRM), genotyping, end point fluorescence, etc.

  • Page 24: Gentier Instrument Installation

    Real-time PCR System B. Gentier Instrument Installation 1. Unpacking Instructions The Gentier instrument transport package is as shown in figure B-1. The Gentier instrument and its accessories are well preserved in a carton case. In order to prevent the collision and oscillation during transportation, the Gentier instrument is sealed with plastic bag and well supported by protective foams.

  • Page 25: Working Environmental Requirements

    Real-time PCR System Reminding: In order to prevent the formation of condensation water, please do not open the transport package until it reaches the room temperature. Reminding: Please check the package integrity before opening it. In case there is any damage, collision or water mark, please contact the transport department or XATL Co., Ltd.

  • Page 26: Power Requirements

    Real-time PCR System 6) For protection against overheating hazards, the openings on Gentier instrument are designed for ventilation. Please do not block or cover these ventilation openings while it is running. 7) Do not place Gentier instrument on soft surface, or the base may sink into the soft surface and block up the air inlet beneath.

  • Page 27: Instrument Installation

    Step: After removing the transport lock, please well reserve it for future use. Then install the fastener back to the original place on the instrument shell, as shown in figure B-2d. Figure B-2. Gentier X3E/Gentier X3R - Remove Transport Lock...
  • Page 28 Step: After removing the transport lock, please well reserve it for future use. Then install the fastener back to the original place on the instrument shell, as shown in figure B-3d. Figure B-3. Gentier X3E/Gentier X3R - Remove Transport Lock Caution: Gentier instrument should be installed by professionals. Unless it is noted in this manual, please do not loosen or remove any internal and external screws or other parts, otherwise, it will break the instrument warranty and result in instrument misalignment.

  • Page 29: External Device Connection

    Real-time PCR System 3.2 External Device Connection Please plug the corresponding power cords of Gentier instrument, control computer and printer into the power supply respectively. Then please confirm the connections of external devices, such as the control computer, display, keyboard, mouse, printer, etc. Reminding: Under normal circumstances, please use the instrument attached power cord.

  • Page 30: Unlock Gentier Instrument

    Real-time PCR System 3.4 Unlock Gentier Instrument At the first startup of Gentier instrument, the instrument system software will prompt user to unlock it, as shown in figure B-4: Figure B-4. System Software - Unlock Gentier Instrument 3.5 Computer Network Settings The network address of the control computer should be set according to the default network information of Gentier instrument so the control computer can be connected to the Gentier instruments under LAN.
  • Page 31 Real-time PCR System step: After the control computer network settings, user can connect Gentier instrument under LAN. For detailed operations, please refer to D-3.1 Menu Bar - Tool (T) submenu- Instrument Management. Figure B-5a. Network Information Window - Network Configuration Information Figure B-5b.
  • Page 32 Real-time PCR System  Subnet Mask: The control computer subnet mask should be same to Gentier instrument’.  Default Gateway: The control computer default gateway should be same to Gentier instrument’s.

  • Page 33: Preparation Before Experiment

    X3S and Gentier X3C, please directly press the Cabin On/Off button in front of instrument. The sample block will go out. Step: Please load the prepared consumables into the sample block, for Gentier X3E and Gentier X3R, tap < Cabin In/Out > function key to close the cabin. For Gentier...
  • Page 34 Real-time PCR System X3S and Gentier X3C, press the Cabin On/Off button in front of instrument to close the cabin.

  • Page 35: Application Software Operation

    Real-time PCR System D. Application Software Operation This section introduces Gentier instrument’s basic functions, including experiment creation, user account management, experiment item settings, experiment run monitoring, experiment data analysis, etc. 1. Application Software Startup After successfully installing the application software on the control computer, user can double click the icon on the desktop or click the application software file on the start menu to start the Gentier instrument application software.
  • Page 36 Real-time PCR System Reminding: User could select not to change the password and directly close the window. Also, user could sign up a new account. Click < Sign Up > and enter the username, password and effective duration to sign up a new account, as shown in figure D-3. After sign-up, user could user the sign-up username and password to log in the system software.

  • Page 37: Main Interface

    Real-time PCR System Figure D-4. Welcome Screen 2. Main Interface After starting the application software, the welcome screen will automatically switch to the main interface which is composed of Menu Bar, Tool Bar and Home Page, as shown in figure D-5.

  • Page 38: Menu Bar

    Real-time PCR System Menu Bar Tool Bar Home Page Figure D-5. Main Interface 2.1 Menu Bar The menu bar of application software includes File, LIS, Option, View and Help. File Sub-menu Options and Function Description 1. New Experiment from Item: User could select a saved experiment item template and create an experiment file with same experiment settings as the selected template.
  • Page 39 Real-time PCR System 7. Save: The edited experiment file can be saved to the default file path. 8. Save as: According to user requirements, save the opened data file to the specified catalog. 9. Report Management: Here could open the corresponding report management system and report file in the experiment file interface.
  • Page 40 Real-time PCR System Figure D-5.2 LIS Configuration Option Sub-menu. Options and Function Description 1. Access Management: Click Access Management option in the Option sub-menu and the application software will automatically pop up < User Management > and < Role Management >. Reminding: This access management function is only viewed and edited by admin account.
  • Page 41 Real-time PCR System ► User: Here displays the added user names in the system. User could click to check this user’s accesses. ► User Information: After user clicking the user name in left side, User Information will be displayed in right side automatically, including username, Admin or not, Expiry Date, Last Login Time, Logins, Note and Associated Role.
  • Page 42 Real-time PCR System Figure D-5.4 Access Management-Role Management ► Role: Here displays the added roles in the system. User could click to check this role’s accesses. ► Role Information: After user clicking the role in left side, Role Information will be displayed in right side automatically, including Role Name, Note and Associated User.
  • Page 43 Real-time PCR System Figure D-5.4a Role Management-Add Role  < Delete Role >: User could delete the role by clicking this function key after selecting a role.  < Revise Role >: User could revise the role information by clicking this function key after selecting a role.
  • Page 44 Real-time PCR System Figure D-5.5 Option Sub-menu - Configuration Management Interface Introduction and Function Description ▶ Instrument List: Here displays the instrument that can be connected, including Instrument Name, Model, SN, IP Address, Status, Experiment Name, Remaining Time and Note. There is <...
  • Page 45 Real-time PCR System Figure D-5.5b Instrument Scanning  < Delete >: User could click this function key to delete the selected instrument.  < View Switch >: User could click this function key to switch instrument view, as shown in figure D-5.6. Figure D-5.6 Instrument View ...
  • Page 46 Real-time PCR System 3. Experiment Data Transmission: Click Experiment Data Transmission option in the Option sub-menu and the application software will automatically pop up the experiment data transmission interface, as shown in figure D-5.7. Figure D-5.7 Experiment Data Transmission The experiment data transmission interface consists of Instrument List, Instrument Experiment File and Local Experiment File.
  • Page 47 Real-time PCR System Figure D-5.8 PANA/Other Device In the PANA/Other device list displays the device Name and IP Address and also the function key of < Add > and < Delete > is in lower right corner.  < Add >: User could click this function key to add a PANA/Other device in its interface after entering PANA/Other device’s IP Address, Name, Username and Password, as shown in figure 5-8a.
  • Page 48 Real-time PCR System Figure D-5.9 Item Management In the item management interface where is item list area and item edit area displays the item file(s) saved in application software. User could import, export, create, delete and edit item files in item management interface. ▶...
  • Page 49 Real-time PCR System Figure D-5.9a Item List Area  < Create >: User could click this function key to create an item file.  < Delete >: User could click this function key to delete the selected item file.  < Import >: User could click this function key to import the item file under local path to item list.
  • Page 50 Real-time PCR System Figure D-5.9b Item Edit Area Description of Created Item File User could click < Create > to enter New Item interface which is composed of General Information and Parameter Analysis and displays General Information tab by default. User could set the parameters and information of new item file here, as shown in figure D-5.9c.
  • Page 51 Real-time PCR System Figure D-5.9c General Information Interface Interface Introduction and Function Description  Item Name: User could enter the new item name here.  Reaction System: User could enter the reaction system for the current item.  Hot Lid: User could enter the hot lid temperature for the current item and check the box to open hot lid.
  • Page 52 Real-time PCR System Figure D-5.9d Parameter Analysis Interface ▶ Analysis Method: User could click key and select the analysis method in the drop-down list, including Absolute Quantitative, Relative Quantitative, Genotyping, End-Point Fluorescence, Melting Curve and High Resolution Melting (HRM) Analysis. The parameter analysis interface will be changed automatically according to the selected analysis method and its parameter settings is are be introduced below.
  • Page 53 Real-time PCR System Figure D-5.9e Parameter Analysis-Absolute Quantitative Interface Interface Introduction and Function Description ▶ Analysis Mode: User could select the analysis mode in light of the experiment type and data analysis requirement, including Baseline Gain Calibration, Isothermal Mode, Hypersensitive Mode, Curve Inversion and Fluorescence Drift Correction. ▶...
  • Page 54 Real-time PCR System Figure D-5.9f Parameter Analysis-Advanced Judgement The advanced judgement tab will be added in the item edit interface after user checking the box of Advanced Judgement, where user could set the parameters of Experiment Contrast and Detection Item, as shown in figure D-5.9g.
  • Page 55 Real-time PCR System Figure D-5.9g Advanced Judgement Interface ▶ Experiment Contrast: The experiment contrast consists of the parameters of Fluorescence, Target Gene, Use, Data Type, Positive Contrast Threshold, Negative Contrast Threshold, Description of the Passed Positive Contrast, Description of the Failed Positive Contrast, Description of the Passed Negative Contrast and Description of the Failed Negative Contrast.
  • Page 56 Real-time PCR System Figure D-5.9h Parameter Analysis-Relative Quantitative Interface Interface Introduction and Function Description ▶ Analysis Mode: User could select the analysis mode in light of the experiment type and data analysis requirement, including Baseline Gain Calibration, Isothermal Mode, Hypersensitive Mode, Curve Inversion and Fluorescence Drift Correction. ▶...
  • Page 57 Real-time PCR System Figure D-5.9i Parameter Analysis- Genotyping Interface Interface Introduction and Function Description ▶ Analysis Mode: User could check baseline subtraction in light of the experiment analysis requirement. ▶ Baseline Settings: There consists of Fluorescence, Target Gene, Manual Baseline, Baseline Initial Value, Baseline End Value.
  • Page 58 Real-time PCR System Figure D-5.9j Parameter Analysis-End-point Fluorescence Interface Interface Introduction and Function Description ▶ Analysis Mode: User could check baseline subtraction in light of the experiment analysis requirement. ▶ Channel Gain: The function and use method channel gain here is same to the one of absolute quantitative.
  • Page 59 Real-time PCR System Figure D-5.9k Parameter Analysis-Melting Curve Interface Interface Introduction and Function Description ▶ Peak Type: User could check positive and negative peak in light of the experiment reagent features and analysis requirement. ▶ Test Target: There consists of Fluorescence, Initial Temperature, End Temperature, Min. -dF/dT and Max.
  • Page 60 Real-time PCR System Figure D-5.9l Parameter Analysis- HRM analysis Interface Interface Introduction and Function Description ▶ Reference Item: User could check Type of Reference Standard, Reference Well and Baseline Group in light of the experiment analysis requirement. ▶ Normalization Parameter: There consists of Fluorescence, target gene, method, Manual Normalization, Start Temperature of Pre-melting, End temperature of Pre-melting, Start Temperature of Post-Melting and End Temperature of Post-Melting.
  • Page 61 Real-time PCR System 6. Standard Curve: Click Standard Curve option in the Option sub-menu and the application software will automatically pop up the standard curve management interface where consists of Standard Curve List and Standard Curve Display Area, as shown in figure D-5.10. Figure D-5.10 Standard Curve Management ▶...
  • Page 62 Real-time PCR System ▶ < Import >: User could click this key to import the standard curve file under local path to the standard curve list. ▶ < Export >: User could click this key to export standard curve to local path. ▶...
  • Page 63 Real-time PCR System 8. Export Graph: Click Export Graph option in the Option sub-menu and the application software will automatically pop up the export graph interface where consists of Export Type, Optional Chart and Export Path, as shown in figure D-5.12. Figure D-5.12 Export Graph Introduction of Parameter and Function Key ...
  • Page 64 Real-time PCR System Figure D-5.13 Export All Data 10. General Settings: Click General Settings option in the Option sub-menu and the application software will automatically pop up the general settings interface where consists of Navigation Page, Password Management, Theme Color, Language, Login Options, Details Settings and Auto Export, as shown in figure D-5.14.
  • Page 65 Real-time PCR System Introduction of Parameter and Function Key ▶ Navigation Page: User could manage the loading of the navigation page under the new experiment item of the application software. User could click the Navigation Page option, then check the Access Navigation Page check box on the right details page to enable the navigation page, and then load the navigation window under the new experiment item, as shown in Figure D-5.14a.
  • Page 66 Real-time PCR System Figure D-5.14b Change Password ▶ Theme Color: User can set the theme color of software interface. Click the Theme Color option and the < Theme Color > key on the right details page, the application software will automatically pop up the Theme Settings window.
  • Page 67 Real-time PCR System Figure D-5.14c Theme Settings Page ▶ Language: The application software provides two system languages: Chinese and English by default, and user can click the Language option and set the system language by checking the corresponding check box. This setting will take effect on the next start of the application software, as shown in figure D-5.14d.
  • Page 68 Real-time PCR System Figure D-5.14d Language Page - General Settings Login Options: User can set the login mode when the software is started. User click the Login Options option and check the Automatic login and Remember Password check box on the right details page to set the login mode as automatic login when the software is started, as shown in Figure D-5.14e.
  • Page 69 Real-time PCR System ▶ Details Settings: User can set the display items of the experimental data and analysis result data page in the Details Settings option, as shown in Figure D-5.14f. User can check the Details Settings check box to select all the details sub-items, or uncheck the Details Settings check box and check the details required to display in the details sub-item list.
  • Page 70 Real-time PCR System Figure D-5.14g Auto Export - General Settings Introduction of Parameter and Function Key  Automatic Export of Experimental Results: User can check the corresponding check box to select the export type of the experimental results, including two types: xlsx and csv. User can export the experiment results to the local specified directory, use the application software default directory or customize the export directory.
  • Page 71 Real-time PCR System Figure D-5.15. Operation Logs Reminding: The Log option appears only when the admin account is logged in. 2. Toolbar: Users can check the Toolbar option, the application software will automatically display the toolbar or uncheck the toolbar, the application software will automatically hide the toolbar.

  • Page 72: Tool Bar

    Real-time PCR System LIS Instruction Manual: Open the export LIS file description for this application software. 3. Software Update: Set up the software online upgrade function. Application software has two update options: Manual Update and Auto-check. Click Manual Update the application software will immediately detect the upgrade version and conduct online upgrade operation;...

  • Page 73: Experiment Tab

    Real-time PCR System user and Switch User shortcut key. User can conduct new experiments, data analysis, instrument management, item management and user switching operations on the home page, as shown in Figure D-6. Figure D-6. Home page Interface 2.3.1 Experiment Tab The Experiment Tab includes four shortcut keys: New Expt.
  • Page 74 Real-time PCR System Experiment Tab ▶ New Expt. from Item : Click this function key, the application software will automatically pop up New Expt. from Item window , the user can enter the Experiment Name, select the Storage Path, select the experimental instrument and the corresponding item file, then click <...
  • Page 75 Real-time PCR System experiment with the same parameters based on the selected experiment file, as shown in Figure D-6.1b. Figure D-6.1b. New Expt. from Existing Expt. File ▶ Based on PANA / Other Expt : Click this function key, the application software will automatically pop up Based on PANA / Other Expt window , the user can enter the Experiment Name, select the Storage Path, select the experimental instrument and the corresponding PANA / other experiment file, then click <...
  • Page 76 Real-time PCR System Reminding: If no PANA / other devices are added in the application software, the application software will pop up adding the relevant PANA devices prompt message first. Click < OK > to enter the PANA / other device management page to add devices. ▶...

  • Page 77: Analysis Tab

    Real-time PCR System Figure D-6.1e. Experimental Navigation- -Add Experiment Program Figure D-6.1f. Experimental Navigation- -Add Sample Information 2.3.2 Analysis Tab The Analysis Tab includes the Open... and Recent experiment files shortcut keys, as shown in Figure D-6.2.

  • Page 78: Instrument Tab

    Real-time PCR System Figure D-6.2. Analysis Tab ► Open...: Click this function key, the application software will automatically pop up the experimental data files selection window. The user can select experimental data file under the saving path of experimental data files, click < Open > to view and analyze the selected experimental data file.
  • Page 79 Real-time PCR System Figure D-6.3. Instrument Tab Instrument Tab ▶ Instrument List: Display the relevant information of the added instrument, including: instrument model, name, and status. ▶ Management: Click this shortcut key, the application software will automatically pop up the Instrument Management window, and the user can add or delete the instrument, as shown in Figure D-6.3a.

  • Page 80: Item Tab

    Real-time PCR System Figure D-6.3a. Instrument Management 2.3.4 Item Tab The Item Tab includes the Item List and the item Management shortcut key, as shown in Figure D-6.4. Figure D-6.4. Item...

  • Page 81: Current Login User And Switch User

    Real-time PCR System Item Tab ▶ Item List: Display the added item files, the user can slide up and down to view all the item files, and double-click the name of the item file to create a new experimental based on this item file. ▶...

  • Page 82: Operation Area

    Real-time PCR System Figure D-6.5a. Switch User Prompt 2.4 Operation Area After creating a new experiment file or open an experiment file, the operation area on the main interface of the application software will be activated, as shown in figure D-7. The new / opened experiment file will be displayed in the experiment file tab below the main interface operation area.

  • Page 83: Experimental Setting

    Real-time PCR System setting in the Experiment Program, Sample Info and Experiment Info functional areas; Real- time Curve and Details functional area monitor running process and analyze the result data after the experimental run. 2.4.1 Experimental Setting Experiment Program The Experiment Program tab is displayed by default in the lower left corner of the application software operation area.
  • Page 84 Real-time PCR System Reminding: User can hover mouse over the target temperature and constant temperature time of a certain step in the temperature program area, and the application software will display the details of this step.  User can double click any step box in the experiment program area and edit the current step in the pop-up Step Settings dialog box.
  • Page 85 Real-time PCR System and Cooling. User can choose the corresponding predefined stage in the stage type window and click < Add > to add the selected stage; or double click any stage to add directly.  User could select Custom Stage and click < Add > to define the stage setting according to specific experiment requirements.
  • Page 86 Real-time PCR System of each cycle, as shown in figure D-7.2a. The experiment temperature procedure will start from the start cycle and each cycle gradually changes the temperature according to the set delta temperature until the step temperature reaches the set target temperature. Reminding: The setting range of Initial Temp and Target Temp is 0.0°C~100.0°C.
  • Page 87 Real-time PCR System Figure D-7.2b. Long Step Model d. Gradient Step Model: Gradient Step Model is divided into two setting models: standard setting and customized setting. The standard setting model is displayed by default, user needs to set the Ramp, Temp. Center and Temp. Offset for the Gradient Step Model, the system will then automatically calculate the corresponding gradient temperatures, as shown in Figure C-7.2c- 1.
  • Page 88 Real-time PCR System  Details: User can click < Details > to view the temperature calculated by system or block temperature customized by user and the gradient temperature distribution map, as shown in Figure D-7.2d. Figure D-7.2d. Gradient Temperature Details e.
  • Page 89 Real-time PCR System Reminding: The setting range of Increment is 0.1° C~5.0° C. f. Continuous Melting: If the current stage is the continuous melting stage, user can double-click the last step of the continuous melting stage and edit this melting step, as shown in Figure D- 7.2f.
  • Page 90 Real-time PCR System ▶ Sample Well Setting Area: User can select the sample wells to set parameters in the sample well setting area, as shown in Figure D-7.3a. Figure D-7.3a. Sample Well Setting Area Introduction to Sample Well Setting Area ▶...
  • Page 91 Real-time PCR System discontinuous columns;  Press [Ctrl] on the computer keyboard, click the row number to select the wells of multiple discontinuous rows;  Select multiple wells of consecutive rows and columns by dragging the mouse on the corresponding rows and columns, drag the mouse on the column number to select the wells of multiple consecutive columns, and drag the mouse on the row number to select the wells of multiple consecutive rows;...
  • Page 92 Real-time PCR System  When user creates a blank experiment and the sample type of the selected well is Non- standard, the fluorescence and Target Gene need to be set, as shown in Figure D-7.3c. Figure D-7.3c. Non-standard Sample Settings - Create Blank Expt. ...
  • Page 93 Real-time PCR System  Concentration Auto-settings: For a series of standard samples diluted by multiple concentration, user can select multiple wells of the standard sample series in the sample well setting area, and use the application software to automatically calculate and set the corresponding standard concentration value.
  • Page 94 Real-time PCR System  When user creates a new experiment from item and the sample type of the selected well is Standard, the Standard Concentration also needs be set, as shown in Figure D-7.3f. Figure D-7.3f. Standard Sample Settings - New Expt. from Item ...
  • Page 95 Real-time PCR System  User can click < Cancel > and the application software will abandon the current standard curve concentration setting and automatically return to the sample information interface. ▶ Clean: User could click this key to clear the related properties settings of the sample of selected wells.
  • Page 96 Real-time PCR System User can set and view experiment information in the Experiment Info tab of operation area, as shown in Figure D-7.4. Figure D-7.4. Experimental Information ▶ Reaction System: User can enter the reaction volume for current experiment in the Reaction System input box and the range is 0μl~100μl.

  • Page 97: Run Monitoring

    Real-time PCR System ▶ User can click the < Fold > or < Unfold > icon to fold or unfold Experiment Info tab. 2.4.2 Run Monitoring After starting experiment, Real-time Curve, Experiment Program, Heat Map and Running Status in operation area is displaying real-time status. 1.
  • Page 98 Real-time PCR System Figure D-7.6. Experiment Program 3. Heat Map The heat map displays the real-time fluorescence heat map of current experiment, as shown in figure D-7.7. Figure D-7.7. Heat Map 4. Running Status Running status displays the instrument information of current experiment, including IP address, instrument name, status and the stage, cycle, step and remaining time of current experiment, as shown in figure D-7.8.

  • Page 99: Result Analysis

    Real-time PCR System Figure D-7.8. Running Status 2.4.3 Result Analysis 2.4.3.1 Absolute Quantification The absolute quantification experiment is intended to quantify the unknown concentration of nucleic acid of sample. Samples with unknown initial nucleic acid quantities are amplified as well as a series of diluted standard samples of known concentrations.
  • Page 100 Real-time PCR System Figure D-8. Absolute Quantification Analysis Reminding: User can select the functional module displayed in this area in the function tag bar above the four areas. Reminding: User can click < Full Screen > icon on the top right corner of each area to display the current functional module in full screen.
  • Page 101 Real-time PCR System ▶ Parameter Introduction:  X-axis represents the cycle number of the current experiment;  Y-axis represents the Relative Fluorescence Unit (RFU); Reminding: User can hover the mouse over a certain amplification curve, the curve will be highlighted and its corresponding well coordinate and endpoint fluorescence value will be displayed on the right of cursor.
  • Page 102 Real-time PCR System IV. Experiment Information: Here displays the related information of current experiment, as shown in figure D-8d. Figure D-8d. Experiment Information V. Standard Curve: Standard curve displays the linear relation between the logarithm value of standard sample’s cycles and the Ct value, as shown in figure D-8e. When quantifies the unknown sample, user could obtain the unknown sample concentration from standard curve in light of Ct value tested from unknown sample.
  • Page 103 Real-time PCR System  Efficiency: It displays the amplification efficiency of standard curve;  R^2: It displays the linear regression coefficient square value of standard curve;  Y-axis intercept: It displays the Y-axis intercept of standard curve; ▶ Function Key Introduction: ...
  • Page 104 Real-time PCR System VII. Raw Curve: Display the diagram of raw amplification curve without subtract baseline, as shown in figure D-8h. The parameters and function keys of raw curve functional module are same with those of amplification curve functional module. Figure D-8h.
  • Page 105 Real-time PCR System Heat Map: Display the Fluorescence heat map, Ct heat map, Concentration heat map, as shown in figure D-8j. Figure D-8j. – Heat Map  Fluorescence: User can select Fluorescence option and select the cycle number from drop- down list;...
  • Page 106 Real-time PCR System Figure D-8k. – Absolute Quantification Analysis Setting ▶ Parameter and Function Key Introduction:  Display Channel: Display all fluorescence channels set in the sample information module by default and user can check the needed fluorescence channels to display. ...
  • Page 107 Real-time PCR System pop up Detailed Analysis Settings interface and user can set the current experiment Analysis Mode and Analysis Method, as shown in figure D-8l. Figure D-8l. – Detailed Analysis Settings ▶ Analysis Mode: Absolute quantification detailed analysis settings interface consists of five analysis modes: Baseline Gain Calibration, Isothermal Model, Hypersensitive Model, Curve Inversion and Fluo.
  • Page 108 Real-time PCR System ▶ Baseline: User can check the different boxes of Baseline Settings Type based on experiment condition, including Gene Group and Well. a. Gene Group: User can set analysis baseline by the gene group; each gene group needs to set a baseline analysis parameter.

  • Page 109: Relative Quantification

    Real-time PCR System Figure D-8o. – Analysis Method  Click <OK> to save current settings after completion.  < Reanalyze >: After setting analysis parameters, user can click < Reanalyze > to reanalyze according to the parameter settings in experiment result analysis interface. 2.4.3.2 Relative Quantification The relative quantification experiment aims to compare the expression level of two or more genes within a same individual.
  • Page 110 Real-time PCR System Reminding: The parameter descriptions of Real-time Curve, Amplification Curve, Experiment Program, Experiment Info, Details, Raw Curve and Sample Info functional modules are same with those introduced in “2.4.3.1 Absolute Quantification”. Interface Introduction and Parameter Description I. Histogram: Display the relative ratio relationship between target gene & sample and reference gene &...
  • Page 111 Real-time PCR System Figure D-9b. Display Settings  Y axis: If user select Linear option, the Histogram functional module will display the linear coordinates of Y axis; if user select Logarithm option, the Histogram functional module will display the logarithm coordinates of Y axis. ...
  • Page 112 Real-time PCR System Figure D-9c. Statistic Result III. Analysis Settings Area: User can set the relevant analysis parameters of relative quantification analysis, as shown in figure D-9d.
  • Page 113 Real-time PCR System Figure D-9d. Relative Quantification Analysis ▶ Parameter & Function Key Introduction:  Display Channel: Display all fluorescence channels set in sample information by default, user can check the needed fluorescence channels to display.  Item Name: Display current experiment relevant item name. User can switch item by clicking drop down list.
  • Page 114 Real-time PCR System  Gene Contrast: User can check the check box of Gene Contrast to load efficiency.  Sample Contrast: User can check the check box of Sample Name to set Reference Sample.  < Detailed Analysis Settings >: User click this key, application software will automatically pop up Detailed Analysis Settings interface.

  • Page 115: Melting Curve

    Real-time PCR System 2.4.3.3 Melting Curve Melting curve shows that the melting degree of dsDNA varies with the rising temperature. Different dsDNA has different melting temperature T (the temperature at which, 50% of the DNA has dissociated from double-stranded to single-stranded) owing to different base sequence, fragment length and GC content.
  • Page 116 Real-time PCR System Figure D-10a. Melting Curve Parameter Introduction:  X axis represents the temperature value;  Y axis represents the Relative Fluorescence Unit (RFU); Reminding: User can hover mouse over a certain melting curve, the curve will be highlighted and its corresponding channel and well coordinate will be displayed on the right of cursor.
  • Page 117 Real-time PCR System III. Analysis Settings area: User can set melting curve relevant parameters, as shown in the figure D-10c. Figure D-10c. Melting Curve Analysis Settings ▶ Parameter & Function Key Introduction:  Display Channel: Display all fluorescence channels set in sample information by default, user can check the needed fluorescence channels to display.

  • Page 118: High Resolution Melting (Hrm) Curve

    Real-time PCR System up Detailed Analysis Settings interface and user can set the current experiment Peak Type and Peak Area, as shown in figure D-10d. Figure D-10d. – Detailed Analysis Settings ▶ Peak Type: User can check the corresponding check box to set the peak type as Positive Peak or Negative Peak.
  • Page 119 Real-time PCR System Normalized Peak Curve, Difference Curve, Details, Normalized Melting Melting, Sample Info and Analysis Settings. The high resolution melting analysis interface is divided into five areas, which displays five functional modules (High Resolution Melting Curve, Difference Curve, Details, Sample Info and Analysis Settings) by default, as shown in figure D-11. Figure D-11.
  • Page 120 Real-time PCR System Figure D-11a. High Resolution Melting Curve ▶ Parameter Introduction:  X axis represents the temperature;  Y axis represents the Relative Fluorescence Unit (RFU); By default, the application software will automatically determine the normalized area of melting curve. The two vertical sliders on the left are the Pre-melting Range threshold lines, which are used to specify the pre-melting temperature range;...
  • Page 121 Real-time PCR System III. Normalized Peak Curve: Display the normalized melting peak curve according to the high resolution melting curve analysis setting, as shown in figure D-11c. Figure D-11c. Normalized Peak Curve Normalized Melting Curve: Display the normalized melting curve according to the high resolution melting curve analysis setting, as show in in figure D-11d.
  • Page 122 Real-time PCR System Figure D-11e. High Resolution Melting Curve Analysis Settings ▶ Parameter & Function Key Introduction:  Display Channel: Display all fluorescence channels set in sample information by default, user can check the needed fluorescence channels to display.  Item Name: Display current experiment relevant item name. user can switch item by clicking drop down list.
  • Page 123 Real-time PCR System  Temp. Compensation: Check the Temp. Compensation, the current experiment will be with temperature compensation; check the Auto Temp. Compensation, the current experiment will be with Auto Temp. Compensation. User can uncheck Auto Temp. Compensation and input manual temp. compensation in the Compensation Threshold edit box below. ...

  • Page 124: Genotyping

    Real-time PCR System according to the parameter settings in experiment result analysis interface. 2.4.3.5 Genotyping The Genotyping analysis adopts two sequence-specific probes labeled with different dyes to identify the wild type and mutation alleles, respectively. After running the experiment program, the application software will automatically detect the end point fluorescence and distinguish different genotypes based on the distribution of two dyes on the scatter plot.
  • Page 125 Real-time PCR System figure D-12a. Figure D-12a. Genotyping Analysis Interface - Scatter Plot Functional Module ▶ Parameter Introduction:  X axis and Y axis represent the Relative Fluorescence Unit (RFU) of different fluorescein respectively;  Each point represents a sample; different icons represent different genotypes or interpretation results.
  • Page 126 Real-time PCR System Figure D-12b. Genotyping Analysis Interface – Analysis Settings ▶ Parameter and function key introduction:  Display Channel: Display all fluorescence channels set in the sample information by default, user can check the needed fluorescence channels to display. ...
  • Page 127 Real-time PCR System threshold will be auto negative threshold. User can uncheck auto negative threshold and input manual negative threshold in following X negative threshold edit box and Y negative threshold edit box to analyses.  Quality threshold: User can set Quality Threshold of sample, the higher quality of sample is, the more reliable of the grouping result is.

  • Page 128: End Point Fluorescence

    Real-time PCR System Figure D-12d. Genomic Baseline Settings b. Well: User can set analysis baseline according to well, each well needs to set a baseline analysis parameter. User can check all rows to keep baseline analysis parameter of all well the same.
  • Page 129 Real-time PCR System divided into five areas, the application software displays five functional modules (Scatter Plot, Experiment Program, Details, Example Info and Analysis Settings) by default, as shown in figure D-13. Figure D-13. End Point Fluorescence Analysis Reminding: The Parameter descriptions of Real-time Curve, Amplification Curve, Experiment Program, Experiment Info, Details, Raw Curve and Example Info functional modules are same with those introduced in “D-3.4.3.1 Absolute...
  • Page 130 Real-time PCR System ▶ Parameter introduction:  X axis represents the sample well index of the current experiment samples;  Y axis represents the End Point Fluorescence (EPF) intensity;  Each point represents a sample; different icons represent the negative or positive interpretation results.
  • Page 131 Real-time PCR System  Florescence: Display current experiment relevant florescence, user can switch florescence by clicking drop down list.  Automatic Baseline: check the Automatic Baseline, the current experiment baseline analysis will be automatic baseline. User can uncheck automatic baseline and set manual baseline analysis in <...
  • Page 132 Real-time PCR System  Click < OK > to save the current settings after the settings are complete.  Reanalyze: After setting analyses parameters, user can click Reanalyze to reanalyze according set parameters in experiment result analysis interface.

  • Page 133: Instrument Software Operation

    Real-time PCR System E. Instrument Software Operation The Gentier X3E and Gentier X3R instrument can run independently from the control computer. User can use the touch screen to operate the instrument system software, to realize the current running and relevant experiment operations. Switch on the power switch and touch control power, the touch screen will display instrument software boot screen accompany with instrument self-inspection.

  • Page 134: Experiment Management

    Real-time PCR System 2. Experiment Management Instrument software operation interface displays experiment management interface by default. Experiment management is consisted of Experiment Management area, Main Operation bar and Running Status bar, as shown in figure E-2. Figure E-2. Experiment Management 2.1 Experiment Management Area Experiment management area is consisted of file display area and seven operation function keys.
  • Page 135 Real-time PCR System Figure E-3. Experiment Management – Running Settings ▶ < New >：User can create a new experiment form item template file or create a new empty experiment.  New Experiment form Item Template File： User can select any item template file; click New and select <...
  • Page 136 Real-time PCR System E-4b. Experiment Management – New – Experiment Name After confirming to create a new experiment, system software switch to Running Settings interface by default. The Running Settings interface for New experiment form item template file and New empty experiment are shown in figure E-3 and E-4c respectively. E-4c.
  • Page 137 Real-time PCR System ▶ < Export/Import >：User can connect USB device to instrument and click icon to export/import item template file or experiment data file, as shown in figure E-6. E-6. Experiment Management – Export/Import  Export File: Export the selected item template file or experiment data file from the instrument system software to the USB device.
  • Page 138 Real-time PCR System Reminding: The system search method is keyword search; the search results display all experiment files that contain keyword input by default. Reminding: If user needs system to display all experiment files after searching, user can clear the contents in search bar and click < Search > to re-display all experiment files. ▶...

  • Page 139: Main Operation

    Real-time PCR System E-9. Experiment Management – Result Analysis 2.2 Main Operation Main operation bar is consisted of Menu Bar, Warning, Experiment Function Keys and Instrument Status. ▶ < Menu Bar >：System software menu bar is consisted of Experiment Management, Running Settings, General Settings, About, Logout and Shutdown, as shown in figure E-...
  • Page 140 Real-time PCR System E-10. Experiment Management – Menu Bar  < Experiment Management >: User clicks Experiment Management and the system software will switch to experiment management interface. See details in “2.1. Experiment Management”.  < Running Settings >: User can click Running Settings to set experiment stage, steps, channels, reaction system and hot lid of experiment program.

  • Page 141: Experiment Running Status

    Real-time PCR System  Experiment Function Keys are consisted of Run Experiment, Pause Experiment Open/Close.  < Run Experiment >: It runs the experiment selected by user in Experiment Management.  < Pause Experiment >: Stop the current experiment.  < Open/Close >: Open or close the loading platform according to the current instrument status.

  • Page 142: Running Settings

    Real-time PCR System E-11a. No Experiment E-11b. Same Experiment E-11c. Different Experiments ： Reminding < Hot Lid > shows the current hot lid status. The flashing icon indicates that the experiment is in heating or cooling status. The normal icon indicates that the experiment is in temperature holding status.

  • Page 143: Temperature Settings

    Real-time PCR System Reminding: After user creates new experiment in Experiment Management interface, system will display running settings interface. When creates new experiment, the experiment stage and step are all blank so user needs to touch function key to add. Reminding ：...
  • Page 144 Real-time PCR System E-13. Running Settings-Temperature Settings Interface Introduction & Function Descriptions 1. Temperature Program Area: Display the current temperature program that is setting, as shown in figure-E14. The upper stage box displays the type and cycles corresponding to each stage.
  • Page 145 Real-time PCR System E-15. Temperature Settings-Stage Settings Box Property Columns & Function Keys Descriptions:  Step: Display each step type of temperature program.  Cycles: Display each step cycles of temperature program.  < Move Up >: Move up the chose step. ...
  • Page 146 Real-time PCR System  < Move down >：Move down the chose step.  < Edit >：Set the parameters for chose step.  < Delete >：Delete the chose step.  < Add >：Add a new step. Temperature Program Settings Step 1: Add Stage User can click <...
  • Page 147 Real-time PCR System Step 2: Edit Stage ▶ The added stage will be shown in Stage Settings box, user can choose any stage to delete it or change its order. ▶ User can choose any stage in Stage Settings box, click < Edit >...
  • Page 148 Real-time PCR System Reminding：user can set only one fluorescence reading step in one stage. Step 5: Edit Step User can select a certain step in the Step Settings box, click < Edit > and the system software will automatically pop up the step edit window, as shown in figure E-19. ▶...
  • Page 149 Real-time PCR System E-19a. Temperature Settings- Step Edit-Standard Step Parameters Settings Reminding: The target temperature setting range is 0.0° C ~100.0° C. Reminding: The constant temperature time setting range is 1s~60min. Reminding: The target speed rate setting range is 0.1° C/s~6.1° C/s. 2) If the Fall step is selected, the step edit window will be shown as figure E-20.
  • Page 150 Real-time PCR System temperature. User can click < Edit > on the right of each parameter and input relevant parameters in input box, as shown in figure E-20a. E-20a. Temperature Settings- Step Edit-Fall Step Parameter Settings Reminding: The start and target temperature setting range is 35.0° C ~100.0° C. Reminding: The temperature change setting range is 0.1°...
  • Page 151 Real-time PCR System E-21. Temperature Setting- Step Edit-Long Step Mode ▶ User needs to set Start Duration and Target Duration for constant temperature duration of long step and set Duration Change of each cycle and Start Cycles of constant temperature Duration.
  • Page 152 Real-time PCR System 4) If the Gradient step mode is selected, the step edit window will be shown as figure E-22a. Gradient Step Model is divided into standard mode and custom mode, standard mode is displayed by default. User can switch modes by clicking the toggle button. E-22a.
  • Page 153 Real-time PCR System ▶ User can switch to custom modes by clicking the toggle button, step edit window is shown in figure E-22c. E-22c. Temperature Settings- Step Edit-Gradient Step Mode-Custom Mode ▶ Custom gradient step mode can adjust temperature of three cabins by six temperature blocks, User can click <...
  • Page 154 Real-time PCR System E-22d. Temperature Settings- Step Edit-Gradient Step Mode- Parameters Setting of Custom Mode Reminding: The block temperature setting range is 0.5° C~99.5° C. Reminding: The other parameter settings are same with the standard step mode. 5) If the selected step is the last step of melting stage, the step will use single fluorescent read mode by default, as shown in figure E-23.
  • Page 155 Real-time PCR System E-23a. Temperature Settings- Step Edit-Melting Step Parameter Settings Reminding: The step temperature setting range is 0.1° C~5.0° C. Reminding: The other parameter settings are same with the standard step mode. 6) If the selected step is the last step of melting stage, the step edit box is shown as figure E- E-24.

  • Page 156: Fluorescence Settings

    Real-time PCR System E-24a. Temperature Setting- Step Edit-continuous melting Step Parameter Setting Reminding: The sampling times setting range is 2 times/° C~15 times/° C. Reminding: The other parameter settings are same with the standard step mode. 3.2 Fluorescence Settings Fluorescence settings interface is consisted of Hot Lid, Reaction System and Fluorescence Settings area.
  • Page 157 ▶ Fluorescence Settings: User can set testing channel in fluorescence setting area.  User can check the box of the channel in Channel column to choose the respective channel. (Channel 1~6 can be checked for Gentier X3E/ Gentier X3S while channel 1~4 can be checked for Gentier X3R/ Gentier X3C.).

  • Page 158: Experiment Monitoring

    Real-time PCR System menu.  User could view Excitation/Detection wavelength under Excitation/Detection column. ▶ After finishing all running settings, user can click < Run > to run the current experiment. 4. Experiment Monitoring After starting experiment, the system will enter the experiment monitoring interface automatically which is consisted of Experiment Monitoring option bar, Experiment Running Status bar and Experiment Running Monitoring diagram, as shown in figure E-26.
  • Page 159 Real-time PCR System  User can press the Temperature option to monitor real-time temperature program of the current experiment 2. Experiment Running Status Bar ▶ Here displays Remaining time, Stage, Cycle and Step of the current experiment. User can check real-time running status of the current experiment. 3.
  • Page 160 Real-time PCR System Figure E-27a. Experiment Running Monitoring Diagram-Heat Map  The heat map displays the 96 sample wells corresponding to the sample block. Cabin I corresponds to 32 wells in columns 1 to 4, cabin II corresponds to 32 wells in columns 5 to 8 and cabin III corresponds to 32 wells in columns 9 to 12.
  • Page 161 Real-time PCR System c. Melting Curve: User can click < Melting > tab on the left of the run monitoring diagram to monitor the real-time Melting curve of the current experiment, as shown in figure E-27c. Figure E-27c. Experiment Running Monitoring Diagram- Real-time Melting Curve ...
  • Page 162 Real-time PCR System Figure E-28b. Experiment Monitoring- Run Monitoring Diagram-Temperature program...

  • Page 163: Result Analysis

    Real-time PCR System 5. Result Analysis User can choose any experiment data file and click < Result Analysis > in experiment management interface to enter result analysis interface where is consisted of Result option bar, Experiment Information bar and Running Result Diagram, as shown in figure E-29. Figure E-29.
  • Page 164 Real-time PCR System 3. Running Result Diagram ▶ After opening experiment data file, user can click Fluorescence in result option bar and click Heat Map, Amplification curve button to check corresponding experiment result data, as shown in figure E-30. When melting experiment is contained in the experiment data opened, user can click Melt on the left of screen to check the corresponding melting curve.
  • Page 165 Real-time PCR System  The fluorescence heat map displays the 96 sample wells corresponding to the sample block, capsule I corresponds to 32 wells in columns 1 to 4, capsule II corresponds to 32 wells in columns 5 to 8, capsule III corresponds to 32 wells in columns 9 to 12; ...
  • Page 166 Real-time PCR System Figure E-30c. Result Analysis- Running Result Diagram-Cabin Display c. Melting Curve: User can click < Melting > on the left of screen when opened experiment data file contains melting experiment, running result diagram will display melting curve of the current experiment data file, as shown in figure E-30d.
  • Page 167 Real-time PCR System  User can click any dye under the Fluorescence option in the run monitoring option bar to check the corresponding real-time melting curve. ▶ Running result diagram will display the temperature program of the current selected experiment data file when user click Temperature option in experiment result bar, as shown in figure E-31.

  • Page 168: General Settings

    Real-time PCR System  The list of Ct value displays the Well information, corresponding fluorescence Channel and Ct Value of the current selected experiment data file.  User can click cabin on the top of Ct value list to check the Ct value of the sample which in this cabin.

  • Page 169: Configuration

    Real-time PCR System Figure e-34. General Settings-Instrument Name Edit Window ▶ Self-Inspection: Display the latest self-inspection result of the current instrument, user can click < Details > to view detailed self-inspection information. ▶ Warning: Display warning information occurred during the self-inspection and experiment running, user can click <...
  • Page 170 Real-time PCR System Figure E-35. General Settings-Network Configuration  User can view all the network parameters and set the corresponding parameter in input box.  User can click < Confirm > to save the current network parameters.  User can press < Close > to chancel edit and turn back to the configuration tab. ▶...

  • Page 171: Service

    Real-time PCR System displayed on the instrument.  User could also click each setting box to input time displayed on the instrument.  User can click Format drop down menu to choose the format of year, month and day displayed on the instrument;...
  • Page 172 Real-time PCR System Figure E-38. General Settings-Add Access After adding access, user should input permission password firstly in experiment management interface and then continue operating, as shown in figure E-38a. Figure E-38a. Experiment Management -Password User can close this permission. User click < Close > in add access and input password in pop up window, click <...
  • Page 173 Real-time PCR System ▶ User Management: The User Management function corresponds to both common user account and Admin account.  Common User Account: User could click < Open > and the common user can change the username and password of the current account by clicking < Edit > and input username and password to be changed in the box, as shown in figure E-39.
  • Page 174 Real-time PCR System  Admin Account: Under the Admin account, the user could modify the password of the current account or add/delete new user by clicking < Open >. The user can click < Edit > button and input password to be modified in box. After input, please click < Save > to save the current modification, as shown in figure E-41, E-42.
  • Page 175 Real-time PCR System Figure E-43. General Settings-User Management-Admin Account-Advanced User Management The Admin user could select and delete any common user by clicking < Delete > or edit the common username and password by clicking < Edit >. After input, please click < Save > to save the current modification, as shown in figure E-44.
  • Page 176 Real-time PCR System Figure E-45. General Settings-User Management-Admin Account-New user ▶ Debug Mode: Click < Enter > and input the password to enter debugging interface. Reminding: No person except the professional engineers from XATL Co., Ltd. is allowed to enter this mode for parameter debugging.

  • Page 177: Instrument Cleaning And Maintenance

    Real-time PCR System F. Instrument Cleaning and Maintenance Under proper use condition, the Gentier instrument requires little maintenance. However, the Gentier instrument should be cleaned and maintained on a regular schedule for long time and constant use. This section includes the information on cleaning and maintenance of the Gentier instrument.

  • Page 178: Clean Touch Screen

    Real-time PCR System Prohibit: Do not spray the detergent directly on the instrument, as malfunctions of the electronics or may occur. Caution: Please do not use organic or strong detergent to clean the instrument shell, which may ruin the surface coating. 1.2 Clean Touch Screen step: Switch off the instrument and unplug the power cord.

  • Page 179: Instrument Maintenance Operation Instructions

    Real-time PCR System 2. Instrument Maintenance Operation Instructions 2.1 Maintain Sufficient Air Flow The placing area of Gentier instrument should be checked regularly, as it requires sufficient air flow to precisely reach the correct target temperature. Please ensure that the air flow is unrestricted and other items around the instrument do not interfering with the air flow.

  • Page 180: Replace Fuse Tube

    Real-time PCR System 2.4 Replace Fuse Tube The fuse tube (Type F10AH250V) of Gentier instrument is located in the fuse tube box near the power outlet at rear of the instrument. Before replacing the fuse tube, please switch off the instrument and unplug the power plug, use a screwdriver to pry open the fuse tube box.

  • Page 181: Gentier Instrument Transportation Locking

    Please load empty PCR 8-tube strip evenly in the sample block, as shown in figure F-2. Figure F-2. Transport Locking Step (1) Step: Touch < Cabin In/Out > function key (Gentier X3E / Gentier X3R) again or press the physical button (Gentier X3S / Gentier X3C) to close the cabin.
  • Page 182 Real-time PCR System Step: For Gentier X3E/Gentier X3R instrument, please touch < Lock > in Service sub-tab under General Settings interface and the system software will conduct “Transport Lock” operation automatically, as shown in figure F-3. For Gentier X3S/Gentier X3C instrument, the after-sale engineer from XATL Co., Ltd.
  • Page 183 Real-time PCR System Figure F-4. Gentier X3E/Gentier X3R Transport Locking Step (3)- Lock Installation...
  • Page 184 Real-time PCR System Figure F-5. Gentier X3S/Gentier X3C Transport Locking Step (3)- Lock Installation Caution: Please first shut down and unplug the instrument and then install the transport lock. Caution: Unless it is noted in this manual, please do not loosen or remove any internal and external screws or other parts of the instrument, otherwise it will break the instrument warranty and result in instrument misalignment.

  • Page 185: Product Packaging

    Real-time PCR System 3.3 Product Packaging Please use the original packaging materials to properly pack the Gentier instrument and its accessories in order to prevent the collision and oscillation during transportation. The specific packing steps of Gentier instrument are shown in figure F-6. Figure F-6.

  • Page 186: Troubleshooting

    Real-time PCR System Reminding: The well packaged Gentier instrument can be transported by general means of transportation (with awnings). 4. Troubleshooting In general, corrective instructions will be display along with the error messages by the application or instrument software. And under normal circumstances the software running errors can be solved by restarting the computer or the instrument system.
  • Page 187 Real-time PCR System  Any housing damage caused by accidentally drop of the instrument;  Obvious functional changes of instrument. Reminding: For a patient/user/third party in the European Union and in countries with similar regulatory regime (Regulation 2017/746/EU on IVD Medical Devices); if, during the use of this device or as a result of its use, a serious incident has occurred, please report it to the manufacturer and/or its authorized representative and to your national regulatory authority.

  • Page 188: Contact Information

    Real-time PCR System G. Contact Information Xi’an Tianlong Science and Technology Co., Ltd. No.4266, Shanglin Road, Weiyang District, Xi'an, 710021, Shaanxi, P.R. China Postcode:710021 Company Tel: +86-29-82682132 Company Fax: + 86-29-82216680 Email Address: inquiry@medtl.com Website Address: www. medtl.net SUNGO Europe B.V.

  • Page 189: Appendix 1 - Product Accessories List

    Real-time PCR System Appendix 1 - Product Accessories list Packing List List Qty. Replacement Period Remark Instrument Power cord Depends on service status 1.5m Network cable Depends on service status 3.0m Application Software USB Depends on service status and FAT32 Flash Disk OEM application update Paper file...
  • Page 190 Xi'an Tianlong Science and Technology Co.,Ltd. Add: No.4266, Shanglin Road, Weiyang District, Xi'an, 710021, Shaanxi, P.R. China Tel: +86-29-8268 2132 Fax: +86-29-8221 6680 https: //www.medtl.net...

This manual is also suitable for:

Gentier x3rGentier x3sGentier x3cGtx3cGtx3sGtx3r ... Show all

Table of Contents