Tianlong GeneRotex 48 User Manual
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Tianlong GeneRotex 48 User Manual

Automatic nucleic acid extractor
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  • Page 2 Automatic Nucleic Acid Extractor Automatic Nucleic Acid Extractor User Manual Created by Xi’an Tianlong Science and Technology Co. Ltd.
  • Page 3 Automatic Nucleic Acid Extractor Disclaimer and Copyright Copyright ©(2015-2022) Xi’an TianLong Science and Technology Co., Ltd. (hereinafter referred to as XATL Co., Ltd.). All rights reserved. All contents of this manual (including but not limited to text, trademark, logo, buttons, icons, graphics, tables, data, etc.) are subject to the copyright and other intellectual...
  • Page 4 Automatic Nucleic Acid Extractor Trademark Registration Statement: is the registered trademark of XI’AN TIANLONG SCIENCE AND TECHNOLOGY CO., LTD. Intended Use Statement: hereinafter referred to The intended use of GeneRotex Automatic Nucleic Acid Extractor ( GeneRotex Extractor) is to complete the automatic extraction and purification of nucleic acid in various samples by using magnetic bead nucleic acid extraction reagent.
  • Page 5 Automatic Nucleic Acid Extractor Safety Symbols and Markings A. Safety Labels on Transport Package Label Description Fragile: the items inside are fragile, please handle with care. This Side Up: indicates the upward side of the transport package. Keep Dry: keep the transport package away from rain or any liquid. IVD Instrument: the product belongs to In Vitro Diagnostic equipment.
  • Page 6 Automatic Nucleic Acid Extractor High Temp.: indicates that a certain area of the GeneRotex Extractor may produce high temperature. Reminding users to pay attention and be careful for burns. Moving Parts: indicates that a certain moving part of the GeneRotex Extractor may cause personal injury.
  • Page 7 Automatic Nucleic Acid Extractor C. Symbols Used in This Manual Symbol Description Caution: reminding user pay attention to a certain operation. Operating the GeneRotex Extractor in any manner unspecified in this manual may results in instrument damage or abnormal function. Reminding: providing important information regarding the operation and successful application of GeneRotex Extractor, including the information explained in further detail elsewhere in this manual.
  • Page 8 Automatic Nucleic Acid Extractor Safety and Precautions The operation, maintenance and repair of GeneRotex Extractor shall strictly follow the safety specifications listed in this section and through this manual. The design of GeneRotex Extractor has fully considered its biological contamination protection, electrical safety protection and mechanical motion protection.
  • Page 9 Automatic Nucleic Acid Extractor Caution: in case of the following situations, immediately cut off the power supply and contact the dealer or ask the Company’s professional maintenance personnel for processing.  Any liquid has entered into the GeneRotex Extractor;  Abnormal sound or smell appears while the GeneRotex Extractor is running; ...
  • Page 10 Automatic Nucleic Acid Extractor III. Electrical Safety and Precautions Prohibit: the voltage of GeneRotex Extractor can cause harms to human body, please cut off the power supply before opening the instrument shell, and it is prohibited to replace any part of the instrument while it is electrified. Caution: the GeneRotex Extractor should be properly grounded, any damage of the internal or external grounding path may cause dangerous.
  • Page 11 Automatic Nucleic Acid Extractor IV. Environmental Safety and Precautions Caution: the GeneRotex Extractor is for indoor use only, the room should be well ventilated and without corrosive gas. Prohibit: never run the GeneRotex Extractor in places that have or may have flammable and explosive gas.

  • Page 12: Table Of Contents

    Automatic Nucleic Acid Extractor Contents 1. INTRODUCTION ........................1 1.1 I ........................1 NTENDED PPLICATION IELDS 1.2 O .............................2 PERATING RINCIPLE 1.3 I ..................3 NSTRUMENT PPEARANCE AND OMPOSITION 1.3.1 Instrument Structure..........................3 1.4 P ..........................5 1.3.2 Experiment Cabin Structure........................4 RODUCT NTRODUCTION 1.4.1 General Parameters of Instrument .....................
  • Page 13 Automatic Nucleic Acid Extractor 3.1 I ..........................17 NSTRUMENT TART 3.2 S ........................17 YSTEM OFTWARE PERATION 3.2.1 Main Interface............................17 3.2.2 Experiment File Tab..........................20 3.2.3 Run Monitoring Tab..........................26 3.2.4 General Setting Tab..........................29 4. INSTRUMENT CLEANING AND MAINTENANCE ............34 4.1 I ................34 NSTRUMENT...
  • Page 14 Automatic Nucleic Acid Extractor 6.3 L ........................45 6.2.2 Reagent and Sample Preparation.....................45 OADING OF LATE 6.4 E ....................46 DIT AND XPERIMENT ROGRAM 6.5 S ..............................47 6.4.1 Edit Experiment Program........................46 HUTDOWN 6.6 M ..........................47 ATTERS FOR TTENTION 7. CONTACT INFORMATION ....................49...

  • Page 15: Introduction

    Automatic Nucleic Acid Extractor 1. Introduction 1.1 Intended Application Fields The GeneRotex Extractor can be widely used in scientific research, clinical diagnosis, disease control, food safety, forensic medicine and other fields. The user only needs to add samples and magnetic bead nucleic acid extraction reagents and load reaction consumables, and then the instrument will complete all nucleic acid extraction operations according to the experimental program and rapidly extract DNA and RNA from animal and plant tissues, blood, body fluids, criminal materials and other samples by using different magnetic bead...

  • Page 16: Operating Principle

    Automatic Nucleic Acid Extractor 1.2 Operating Principle The GeneRotex Extractor is designed as a high-tech product to extract the nucleic acid through magnetic beads method. It is characterized as high degree of automation, fast extraction speed, reliable results and easy operation. By utilizing of 48 deep well plates, it is capable of simultaneously deal with 1 to 48 samples.

  • Page 17: Composition

    Automatic Nucleic Acid Extractor 1.3 Instrument Appearance and Composition 1.3.1 Instrument Structure Figure 1-3. GeneRotex Automatic Nucleic Acid Extractor - Front View Figure 1-4. GeneRotex Automatic Nucleic Acid Extractor - Rear View...

  • Page 18: Experiment Cabin Structure

    Automatic Nucleic Acid Extractor 1.3.2 Experiment Cabin Structure Figure 1-5. GeneRotex Extractor - Experiment cabin 1. Operation screen 2. Experiment cabin lifting window 3. Experiment cabin overhead door 4. Heat dissipation opening 5. Power switch 6. Fuse tube holder 7. Power socket 8.

  • Page 19: Roduct Ntroduction

    Automatic Nucleic Acid Extractor 1.4 Product Introduction 1.4.1 General Parameters of Instrument Dimension:490mm(L) × 510mm (W) × 480mm (H); ▶ Instrument Specification: Weight: 45kg; Dimension: 700mm(L) × 660mm (W) × 580mm (H); ▶ Package Specification: Weight: 55kg; Voltage: AC 100V-240V; ▶...

  • Page 20: Performance Parameters Of Instrument

    Automatic Nucleic Acid Extractor 1.4.2 Performance Parameters of Instrument Well 1 is 50μL to 3000μL; Well 2-5 is 50μL to 1500μL; Well 6 is 30μL to 1000μL. ▶ Processing Volume: 1-48 fluxes; ▶ Sample Flux: CV <3% (For identical concentration samples that are extracted in identical process). ▶...

  • Page 21: Software System Characteristics

    Automatic Nucleic Acid Extractor 30-60 minutes per time (depending on the reagent used), at most 48 samples can be ▶ Rapid Extraction: extracted simultaneously. The extraction protocol can be optimized according to the reagents for more precise ▶ Extraction Efficiency: incubation time.

  • Page 22: Reagent Characteristics

    Automatic Nucleic Acid Extractor Multiple languages can be dynamically switched, and the default languages are ▶ System Language: Chinese and English. 1.4.5 Reagent Characteristics Open Platform: various magnetic bead nucleic acid extraction reagents can be used. ▶ 1.4.6 Consumable Specification 48 wells: 48-deep well plate, stirring sleeve.

  • Page 23: Unpacking And Installation

    Automatic Nucleic Acid Extractor 2. Unpacking and Installation 2.1 Unpacking Instructions The GeneRotex Extractor and its accessories are well preserved in a carton case. In order to prevent the collision and oscillation during transportation, the GeneRotex Extractor is sealed with plastic bag and well supported by protective foams. Reminding: in order to prevent the formation of condensation water, please do not open the transport package until it reaches the room temperature.

  • Page 24: Unpacking Steps

    Automatic Nucleic Acid Extractor 2.1.1 Unpacking Steps Step: Transfer the GeneRotex packaging to the work place, as shown in Figure 2-1a. Step: Open the upper cover of the packing box, take out the instrument accessories and documents accompanied by the instrument, and remove the protective foam on the top of the instrument, as shown in Figure 2-1b and Figure 2-1c.
  • Page 25 Automatic Nucleic Acid Extractor Figure 2-1. GeneRotex Extractor - Instrument Unpacking...

  • Page 26: Orking Nvironmental Equirements

    Automatic Nucleic Acid Extractor 2.2 Working Environmental Requirements 2.2.1 Space Requirements of Instrument The GeneRotex Extractor should be placed in the room with low humidity (≤80%RH) and appropriate temperature (between 10°C~30°C). The room should be well ventilated and without corrosive gas. The GeneRotex Extractor should be placed on steady lab work bench and leave enough space for placing the attachments, cables and reagent bottles, etc.
  • Page 27 Automatic Nucleic Acid Extractor The power specifications of GeneRotex Extractor are listed in Parameters”, use improper “A3.1 Instrument power may damage the circuit system and cause fire, it is recommended to use UPS Parameters and Characters - 3.1.1 Instrument General power supply.

  • Page 28: Nstrument Nstallation

    Automatic Nucleic Acid Extractor 2.3 Instrument Installation 2.3.1 Remove Transport Lock After unpacking, please do not directly power on the GeneRotex Extractor, it is fixed by transport lock during transportation. Please remove the transport lock in accordance with the instructions as below before running the GeneRotex Extractor. Figure 2-2.

  • Page 29: Instrument Self Inspection

    Automatic Nucleic Acid Extractor Step: Please open the experiment cabin. There are two transport locks fixed under the movable loading platform, as shown in Figure 2-2. Step: Remove two screws on each transport lock with a screwdriver and remove the transport lock, as shown in Figure 2-2.
  • Page 30 Automatic Nucleic Acid Extractor Step: before carrying out the experiment, it is suggested to Run the “demo experiment program” first, in order to observe whether the instrument can run normally. After it is normal, the user experiment program can be run. Caution: before power on the GeneRotex Extractor, please ensure: ...

  • Page 31: Instrument Operation Instructions

    Automatic Nucleic Acid Extractor 3. Instrument Operation Instructions The GeneRotex Extractor can be operated independently with the instrument operation screen and requires no computer. This section includes the function descriptions of the GeneRotex Extractor and its operational process. Please carefully read the instructions provided herein to achieve related experimental operations.
  • Page 32 Automatic Nucleic Acid Extractor Figure 3-1. GeneRotex System Software - Main Interface Interface Introduction & Parameter Descriptions Status Bar: display the system status, current experiment file name, instrument status and system time. System Status: display the current instrument system status. ...
  • Page 33 Automatic Nucleic Acid Extractor Operation Area: the operation area of the system software includes three tabs: Experiment File, Run Monitoring and General Setting. Users could perform relevant operations and set the corresponding contents according to the specific experimental requirements. Experiment File: display experiment file icons and provide the relevant experiment file operations;...

  • Page 34: Experiment File Tab

    Automatic Nucleic Acid Extractor 3.2.2 Experiment File Tab The main interface of the instrument system software displays experiment file tab by default. The experiment file interface will display file name, file icon and operation function buttons, as shown in Figure 3-2. 1.png Figure 3-2.
  • Page 35 Automatic Nucleic Acid Extractor Figure 3.2b Experiment File Interface - Experiment file display area Reminding: the GeneRotex System Software include following two types of files: Lamp>: this icon indactes the UV disinfection file, which is provided by default and cannor deleted by user. The user can click this icon to edit the corresponding UV ...
  • Page 36 Automatic Nucleic Acid Extractor < Scan >: the GeneRotex Extractor instrument can be connected to the two-dimensional code scanner through the USB Interface. Before starting the ▶ experiment, click < Scan > to scan the two-dimensional code of the reagent kit, for detail operation please refer to “6.2.1 Scan Reagent Kit Code”.
  • Page 37 Automatic Nucleic Acid Extractor Figure3.2d Experiment File Interface - Keyboard 3.2.2.2 Edit Experiment File The user can click < Edit > key to edit the selected experiment file, and then the system software will automatically enter the experiment edit interface, as shown in Figure 3-3. Figure 3.3 GeneRotex System Software - Experiment File Edit Interface Reminding: <...
  • Page 38 Automatic Nucleic Acid Extractor Interface Introduction & Parameter Descriptions Experiment Step Parameters: display the parameter information of all steps of the current experiment program, including: ▶  Step: displays the sequence of current step.  Name: displays the name of the current step. ...
  • Page 39 Automatic Nucleic Acid Extractor Figure 3.3b Experiment File Edit Interface - Well Setting Figure 3.3c Experiment File Edit Interface Figure 3.3d Experiment File Edit Interface Time Setting Stirring Speed Setting Figure 3.3e Experiment File Edit Interface Figure 3.3f Experiment File Edit Interface Volume Setting Temperature Setting Reminding: the wells in the experimental procedure step can be set to 1-6 wells;...

  • Page 40: Run Monitoring Tab

    Automatic Nucleic Acid Extractor Reminding: the setting range of volume of the experiment step is 30-1000μL; Reminding: the setting range of lysis or eluting temperature of the experiment step is 30-120℃; User can click any step number to select the corresponding experiment step, and click <...
  • Page 41 Automatic Nucleic Acid Extractor Figure 3-4. Run Monitoring Tab - Experiment Information Interface Introduction & Parameter Descriptions Real-time Status: displays the real-time status of the current experiment.  Current Step: displays the current running step number;  Step Name: displays the current running step number; ...
  • Page 42 Automatic Nucleic Acid Extractor Running Information: displays the progress of the current running experiment in the form of percentage progress bar. Experiment Information: Click < Expt. Info > the run monitoring interface will display the remaining experiment time of the current experiment file, the experimental procedure, the parameters of each procedure step and other corresponding experiment information, as shown in Figure 3-4a.

  • Page 43: General Setting Tab

    Automatic Nucleic Acid Extractor 3.2.4 General Setting Tab 3.2.4.1 Instrument Setting Sub-tab When the user clicks General Setting tab on the system software interface, the system software will automatically enter the general setting interface and display the relevant information and system parameters of the current instrument. The general setting interface consists of Instrument and Service two sub-tabs, and displays the Instrument sub-tab by default, as shown in figure 3-5.
  • Page 44 Automatic Nucleic Acid Extractor Date and Time: users can press < Set > to set the date and time displayed on the current GeneRotex Extractor, the date/time setting window will pop up as shown in ▶ Figure 3.5a. Figure 3.5a Instrument Setting Sub-tab -- Date/Time Setting ...
  • Page 45 Automatic Nucleic Acid Extractor 3.2.4.2 Services Setting Sub-tab Click Services sub-tab in the general setting interface, and the system software will automatically enter the services setting sub-tab, as shown in Figure 3-6. Services Details Details Figure 3.6 General Setting Tab -- Services Setting Sub-tab Interface Introduction &...
  • Page 46 Automatic Nucleic Acid Extractor Scan Log: user can click < Details > to view the detailed scan log of the current instrument, as shown in Figure 3.6b. ▶ Figure 3.6b Services Setting Sub-tab - Details of Instrument Scan Log Cumulated Disinfection Time: displays the cumulated disinfection time of the current instrument.
  • Page 47 Automatic Nucleic Acid Extractor users can click < Clean >, and the stirring sleeve will descend to the lowest position, ▶ Stirring Sleeve: which is convenient for users to clean the stirring sleeve. After finishing the cleaning,  users can click < Confirm > to make the stirring sleeve reset to the original position automatically.

  • Page 48: Instrument Cleaning And Maintenance

    Automatic Nucleic Acid Extractor 4. Instrument Cleaning and Maintenance Under proper use condition, the GeneRotex Extractor requires little maintenance. However, the GeneRotex Extractor should be cleaned and maintained on a regular schedule for long time and constant use. This section includes the information on cleaning and maintenance of the GeneRotex Extractor.

  • Page 49: Clean Instrument Shell

    Automatic Nucleic Acid Extractor 4.1.1 Clean Instrument Shell step: switch off the instrument and unplug the power cord; step: clean the instrument shell with a piece of damp, soft cloth, and if needed, please rinse the cloth with mild commercial detergent for cleaning. Prohibit: do not spray the detergent directly on the instrument, as malfunctions of the electronics or may occur.

  • Page 50: Nstrument Maintenance Operation Instructions

    Automatic Nucleic Acid Extractor step: clean magnetic bar, magnetic bar frame and stirring sleeve support in the experiment cabin with absorbent cotton or cotton cloth dipped in SDS solution, 75% ethanol or soapy water. Caution: do not clean the magnetic bar with metal instruments to protect the bar from damage.

  • Page 51: Transport And Return For Maintenance

    Automatic Nucleic Acid Extractor Caution: Although the instrument has passed the safety certification and test, when the experiment cabin is cleaned, it is strictly forbidden for the cleaning personnel to clean the instrument while it is running or in standby state, so as to avoid damaging the instrument or injuring the cleaning personnel.

  • Page 52: Uv Disinfection

    Automatic Nucleic Acid Extractor ▶ After wiping with disinfectant and drying the disinfectant inside the experiment cabin, clean the inside of the experiment cabin with 75% ethanol again, and clean the surface of the instrument with conventional cleanser. ▶ After the ethanol in the experiment cabin and the cleanser on the surface of the instrument are dried, disinfect the instrument with UV for 2 hours as suggested.

  • Page 53: Installation Of Transport Locks

    Automatic Nucleic Acid Extractor Cancel Figure 4-1 UV Disinfection Time Setting 4.3.3 Installation of Transport Locks When the instrument is required to be transported again, please pack and transport the instrument in strict accordance with the requirements for instrument transportation in order to avoid or reduce instrument damage in the process of transportation.
  • Page 54 Automatic Nucleic Acid Extractor Figure 4-2 Installation of Transport Locks Caution: unless it is noted in this manual, please do not loosen or remove any internal and external screws or other parts, otherwise it will break the instrument warranty and result in instrument misalignment.

  • Page 55: Product Packaging And Return For Maintenance

    Automatic Nucleic Acid Extractor 4.3.4 Product Packaging and Return for Maintenance If the instrument is required to be returned to the agent or after-sales service department, please pack and return it according to the following procedures: 1) Please thoroughly clean the instrument. 2) Please fully disinfect the interior of the experiment cabin according to instructions mentioned in this user manual and provide disinfection certificate.

  • Page 56: Troubleshooting

    Automatic Nucleic Acid Extractor 5. Troubleshooting In general, corrective instructions will be display along with the error messages by the application or instrument software. And under normal circumstances the software running errors can be solved by restarting the computer or the instrument system. This section describes the main possible errors of the GeneRotex Extractor together with possible causes and corrective instructions.
  • Page 57 Automatic Nucleic Acid Extractor The magnetic bar Contact our company or agent drops system Misuse or misoperation Cut off the power supply and restart halted or out of the instrument control Others Contact our company or agent Reminding: The fuse tube of GeneRotex Extractor is a F10AH250V fuse tube and it is installed between the power socket and the switch at the backside of the instrument.

  • Page 58: Experiment Operation Guide

    Automatic Nucleic Acid Extractor 6. Experiment Operation Guide 6.1 Overview of Experiment Operation Guide The purpose of this experiment operation guide is to guide users to combine the use of the Automatic GeneRotex Extractor with its reagents, quickly understand and be skilled at the operation procedure.

  • Page 59: Reagent And Sample Preparation

    Automatic Nucleic Acid Extractor Before starting the experiment, click < Scan > and the main interface will pop up the dialog box, as shown in Figure 6.2. User can use the code scanner to scan the QR code on the nucleic acid extraction reagent kit.

  • Page 60: Edit And Run Experiment Program

    Automatic Nucleic Acid Extractor Figure 6.3 Deep Well Plate 6.4 Edit and Run Experiment Program 6.4.1 Edit Experiment Program 1) Please connect the power supply and turn on the power switch. 2) Please click Experiment File tab on the main interface to enter the experiment file interface and create a new experiment file on the experiment file interface.

  • Page 61: Hutdown

    Automatic Nucleic Acid Extractor 6.5 Shutdown The power switch is located on the back of the instrument. The user can switch the power switch to the end marked with “O” to turn off the GeneRotex Extractor Do not cut off the power supply of the instrument if there is no accident or failure Reminding: during the experiment, so as not to affect the experiment.
  • Page 62 Automatic Nucleic Acid Extractor When using the GeneRotex Extractor for the first time, do not power on the instrument directly. Please remove the transport locks according to the instructions before starting the operation. For detailed methods, please refer to in the manual . “...

  • Page 63: Contact Information

    Automatic Nucleic Acid Extractor 7. Contact Information Xi’an Tianlong Science and Technology Co., Ltd. No. 389, Zhuhong Road, Xi’an, Shaanxi Province, P. R. China. Postcode: 710018 Company Tel: +86-29-82218051/83204255 Company Fax: + 86-29-82216680 After-sale Service: + 86-29-82683675 Email Address: XATL@medtl.com Website Address: www.
  • Page 64 Add:No.389, Zhuhong Road, Xi’an, Shaanxi Province, P.R.China...

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