CWE MM-100 Instruction Manual
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CWE MM-100 Instruction Manual

Metabolic monitoring system
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Instruction Manual
MM-100
Metabolic Monitoring System
Read instructions carefully before operating this device.
This device is not to be used for Human Life Support applications.
To avoid possible electrical shock, do not operate this device if is wet or has had
liquids spilled onto it.
Service or calibration procedures should only be performed by qualified
personnel familiar with the electrical hazards of line-powered devices.
Ardmore PA 19003 U.S.A.
(610)642-7719
info@cwe-inc.com
www.cwe-inc.com

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  • Page 1 Instruction Manual MM-100 Metabolic Monitoring System Read instructions carefully before operating this device.  This device is not to be used for Human Life Support applications.  To avoid possible electrical shock, do not operate this device if is wet or has had liquids spilled onto it.
  • Page 2 LIFE SUPPORT POLICY Instruments manufactured by CWE, Incorporated are not authorized for use as critical components in human life support devices or systems. "Life support devices or systems", as used herein, are devices or systems whose failure to perform, whether through misuse, failure, or proper operation, can reasonably be expected to result in significant injury to the operator or subject persons.

  • Page 3: Table Of Contents

    MM-100 Metabolic Monitor Instruction Manual TABLE OF CONTENTS 1.0 INTRODUCTION ....................... 5 2.0 QUICK START GUIDE ....................6 3.0 DETAILED SETUP (SINGLE CHAMBER SYSTEM) ..........8 3.1 DETAILED SETUP (MULTI CHAMBER SYSTEM) ..........10 4.0 OVERALL OPERATION ..................12 5.0 MAINTAINING THE DESICCANT (CHAMBER OR TUBE) ........14 6.0 FRONT PANEL DISPLAY AND CONTROLS ............
  • Page 4 Figure 2: Mouse cage showing airflow & sample connections & thermistor probe attachment........................8 Figure 3: Side view of MM-100 showing desiccant tube. Required in-line filter to the right. The Dri-Tech Desiccant Chamber connects in the same manner to the two ports. 9 Figure 4: MM-100 single-animal tubing connection diagram ........

  • Page 5: Introduction

    When expanded to multi-animal monitoring (using the MMX-8 expansion unit), the gas measurements are still made by the host MM-100. Air flow through each cage is measured in the expansion unit. This plan ensures reproducibility and direct comparison between animals, since all measurements are made with the same set of calibrated sensors.

  • Page 6: Quick Start Guide

    Connect CWE’s Dri-Tech Desiccant Chamber to the MM-100 using the enclosed Dri-Tech Instruction Manual. Alternatively, connect a fresh desiccant tube on the side of the MM-100. Be sure that the small Acrodisk hydrophobic filter is connected between the desiccant chamber or tube and the side panel tubing port (near the rear panel).
  • Page 7 COM PORT number. The textbox dropdown menu will show all active COM ports on your computer, so be sure to select the one connected to the MM-100. The program will not work if the correct COM port is not selected! 8.

  • Page 8: Detailed Setup (Single Chamber System)

    5. Plug the provided YSI-402 Thermistor Probe into the jack on TEMPERATURE PROBE the rear panel of the MM-100. The probe tip should be inserted in its holder on the lid of the monitoring chamber. This provides ambient temperature measurement for the metabolic calculations.
  • Page 9 MM-100 system. OR, in place of the Dri-Tech Desiccant Chamber, you can install a fresh desiccant tube on the side of the MM-100. It is recommended that a spare desiccant tube be kept handy for replacement as needed.

  • Page 10: Detailed Setup (Multi Chamber System)

    (all are assumed to be at the same temperature). 6. Be sure that a fresh Dri-Tech Desiccant Chamber OR a fresh desiccant tube is connected on the side of the MM-100. It is recommended that a spare desiccant tube be kept handy for replacement as needed.
  • Page 11 MM-100 Metabolic Monitor Instruction Manual 8. After the unit has warmed up and completed its initial calibration, adjust the CAGE (about 650ml/min for mouse, 1500ml/min for rat) for each animal being FLOW monitored. This can be accomplished by switching the MMX to...

  • Page 12: Overall Operation

    Startup: After the MM-100 has just been powered on, it will automatically wait for the flow and gas sensors to warm up and stabilize. This normally takes between five and ten minutes.
  • Page 13 CHECK SENSOR FLOW will blink. The most common cause for this is a blocked hydrophobic disk filter. It is a good idea to have some spare disk filters available when the MM-100 is in operation. Software: The supplied MM-CommVS software is used to monitor current values of the gasses, air flow, temperature, etc., that are sent once-per-second from the MM-100.

  • Page 14: Maintaining The Desiccant (Chamber Or Tube)

    MM-100 Metabolic Monitor Instruction Manual 5.0 MAINTAINING THE DESICCANT (CHAMBER OR TUBE) For accurate measurements, it is essential that the gas sample be dry. This is accomplished with the external Dri-Tech Desiccant Chamber (or a desiccant drying tube) that is connected to the side of the instrument. One Dri-Tech Desiccant Chamber with fresh desiccant medium will typically last approximately 10 –...

  • Page 15: Indicator Lights

    MM-100 Metabolic Monitor Instruction Manual The first two lines display the current O and CO values. The third line shows the barometric pressure in millibars and the chamber temperature in degrees Centigrade. The bracketed number at top right shows the measurement chamber being monitored.

  • Page 16: O2 And Co2 Calibration Pushbuttons

    REFERENCE AIR INLET readings and can permanently damage the gas sensors. ALWAYS allow the MM-100 to draw in the calibration gas at its own rate from a stream of gas. This can easily be accomplished with a simple “T” arrangement at the sample port.

  • Page 17: Co2 Low Calibration

    MM-100 Metabolic Monitor Instruction Manual 6.311 CO2 LOW CALIBRATION is used to set the low calibration point for CO measurement. Typically, this is set to about 0.044% when sampling atmospheric air. 6.312 CO2 HIGH CALIBRATION is used to set the high calibration point for CO measurement.

  • Page 18: Setting The Number Of Cages

    3 minutes (180 seconds) is adequate. 6.324 CAL / MONITOR SWITCH switch is used to manually select calibration mode. MONITOR Pressing this switch causes the MM-100 to enter calibration mode, which is indicated by the blinking blue LED. A message is also displayed CALIBRATING...

  • Page 19: Cage Airflow Adjust

    MM-100 Metabolic Monitor Instruction Manual via the knob. The room air values are stored and used in the ADJUST metabolic calculations as “ambient air” values. 6.4 CAGE AIRFLOW ADJUST This knob adjusts the needle valve that controls the fresh airflow through the animal measurement chamber.

  • Page 20: Analog Output Jacks

    MMX to the SAMPLE OUTLET SAMPLE INLET the MM-100 rear panel. The gas sample path is thus from the animal cages to the MMX expansion unit, where valves direct it to the main sample inlet on the MM-100 for measurement.

  • Page 21: Expansion Port (Db25)

    MM-100 Metabolic Monitor Instruction Manual Control bit 4 is a master remote control bit. Setting this high sets the MM-100 into remote-control mode. Control bits 0 - 3 are used to supply a binary digital number that controls which cage to monitor. For example, 0000 = cage 1, 0001 = cage 2,…...

  • Page 22: Restore Factory Settings

    MM-100 Metabolic Monitor Instruction Manual 9.1 RESTORE FACTORY SETTINGS This function returns all the calibration and setup settings to the original factory values. This can be useful, for example, if a gas calibration has been dialed well outside its normal range. If this function is used, a complete gas calibration must be done.

  • Page 23: Specifications

    MM-100 Metabolic Monitor Instruction Manual 10.0 SPECIFICATIONS Oxygen analyzer type ................paramagnetic Linearity (0 - 100%) ..................+0.1% O Repeatability ..................< 0.01% O /hour Zero stability ................... +0.002% O /hour Response time ..................2.0 seconds Operating temperature .................. 0 - 65 Maximum pressure ...................

  • Page 24: Mm-Commvs Software

    11.0 MM-CommVS SOFTWARE The MM-CommVS software provided with the system computes the metabolic measurements from the gas and flow values provided by the MM-100 and records the measurements to disk files for further analysis. Assuming the software has been successfully installed, the following description will outline its various functions.
  • Page 25 MM-100 Metabolic Monitor Instruction Manual Menu Commands File menu – Use this to Open or Close a data file. Data files can be named anything you wish but will always be saved with a .txt extension. If a previously existing file is selected the new data will be appended to the end of the file. The...
  • Page 26 MM-100 Metabolic Monitor Instruction Manual Configuration menu Show weights – Selecting this will bring up a new window where the user can enter the weight in grams for all animals in the study (up to 8). With a single- chamber system only Animal #1 is relevant. These weights allow the program to calculate standardized values for VO , heat production, etc.
  • Page 27 COM PORT – This drop-down text box is used to select the COM port to be used for communicating with the MM-100 monitor. When MM-CommVS starts, all the available COM ports are identified and listed in this drop-down control. If you have more than one active COM port, be sure to select the one connected to the MM-100.
  • Page 28 MM-100 Metabolic Monitor Instruction Manual Checkbox Controls Show RER – When checked, RER values are displayed on the graphics screen with the VO2 and VCO2 traces. Show gas deltas – When checked, the differences between input and output gas concentrations are displayed with the VO2 and VO2 traces. This provides an indication of appropriate fresh air flow through the measurement chamber;...

  • Page 29: Ordering Information

    MM-100 Metabolic Monitor Instruction Manual 12.0 ORDERING INFORMATION Part No. Description ========= ================================================ 11-20000 MM-100 system for one animal 11-23000 MMX-8 Eight animal expansion unit 11-20100 Mouse chamber 11-20110 Rat chamber 11-20222 Dri-Tech drying chamber kit, includes 1lb Drierite™ 11-20211 Drierite™, 1lb (0.45kg) granular desiccant...

  • Page 30: Appendix A: Oxygen Consumption Calculations

    MM-100 Metabolic Monitor Instruction Manual Appendix A: Oxygen Consumption Calculations The MM-100 relies on the well-known open-circuit method of measuring oxygen consumption (VO ) and carbon dioxide production (VCO ). This method requires the measurement or derivation of six variables:...

  • Page 31: Appendix B: Tables

    MM-100 Metabolic Monitor Instruction Manual Appendix B: Tables Table 1: The principal components of atmospheric air: Constituent Formula Percent by volume dry air Water vapor variable Nitrogen 78.084 Oxygen 20.948 Carbon Dioxide 0.0315 Argon 0.934 Helium 0.000524 Neon 0.001818 Table 2: Typical metabolic measurements for small animals:...

  • Page 32: Preparing The Dri-Tech Desiccant Chamber

    MM-100 Metabolic Monitor Instruction Manual Appendix C: Desiccant chambers and tubes The Dri-Tech Desiccant Chamber and desiccant tubes supplied with the MM-100 system serve an important purpose: to remove water vapor from the metabolic gas sample. This process is essential for accurate metabolic measurements, which require a “dry”...
  • Page 33 MM-100 Metabolic Monitor Instruction Manual migration of the indicator into the interior of the granule and sublimation of the indicator, but it will still retain its water-absorbing properties. It is a good idea to have one or more freshly charged desiccant tubes handy for quick replacement of a spent tube.

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