BioLAB BSDBU-201-A Operation Manual
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BioLAB BSDBU-201-A Operation Manual

Double beam uv visible spectrophotometer
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Operation
Manual
BSDBU-201-A
Double Beam UV Visible
Spectrophotometer
Thank you for Choosing Biolab products. Please read the "Operating Instructions" and
"Warranty" before operating this unit to assure proper operation.

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Summary of Contents for BioLAB BSDBU-201-A

  • Page 1 Operation Manual BSDBU-201-A Double Beam UV Visible Spectrophotometer Thank you for Choosing Biolab products. Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper operation.

  • Page 2: Table Of Contents

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Index 01 Safety ................................03 02 General ..............................03 03 Electrical ..............................03 04 Warning ..............................04 05 Performance ............................04 06 Radio Interference ..........................04 07 Introduction ............................05 08 Working Principle ..........................06 09 Unpacking Instructions ........................07 10 Unpacking Instructions ........................08 11 Operation ...............................08...

  • Page 3: Safety

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Safety The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. This instrument should be operated by appropriate laboratory technicians.

  • Page 4: Warning

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Warning Any interruption of the protective conductor inside or outside the apparatus or disconnection of the protective earth terminal is likely to make the apparatus dangerous. Intentional interruption is prohibited. Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation.

  • Page 5: Introduction

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Introduction This instrument (Fig 1) is a double beam, general purpose instrument designed to meet the needs of the Conventional Laboratory, This instrument is ideal for various applications, such as: Chemistry, Biochemistry, petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control and research.

  • Page 6: Working Principle

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig1 Working Principle The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps to supply the light; 2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation; 3) A sample compartment to accommodate the sample solution; 4) Two detectors to receive the transmitted light and convert it to an electrical signal;...

  • Page 7: Unpacking Instructions

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition. Packing List Description Quantity • Spectrophotometer • Mains Lead •...

  • Page 8: Unpacking Instructions

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Unpacking Instructions 1. After carefully unpacking the contents, check the materials with the packing list (page 4) to ensure that you have received everything in good condition. 2. Place the instrument in a suitable location away from direct sunlight. In order to have the best performance from your instrument, keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high-frequency fields.
  • Page 9 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 3  Description of keys Load data or curve saved before; 【LOAD】 Save data or curve; 【SAVE】 Set wavelength; 【SETλ】 Blank or scan the user base line; 【ZERO】 Print test results or screen 【PRINT】...
  • Page 10 Double Beam UV Visible Spectrophotometer BSDBU-201-A 【∧】,【∨】 Change “y” scale; Search peak after scan; Scroll items for selecting; Change capital/small letter last typed in; Browse the items for selection; 【CELL】 Set cell position (Only available when Auto Changer used). 【HELP】 Reserved key for future Function Extending, not available now.
  • Page 11 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 4A Fig 5 Fig 6 Fig 7 www.biolabscientific.com...
  • Page 12 Double Beam UV Visible Spectrophotometer BSDBU-201-A • Basic operation ※ Blank There is a system baseline stored in the memory of the instrument. Usually user may not rebuild system baseline before test. Only putting the sample into the sample light path and the reference into the Reference Light Path, the result can be obtained.
  • Page 13 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 9 b. Take out the sample cuvette, replace the reference solution with sample solution after flushing the cuvette completely.Put the sample cuvette into the Sample Light Path, then the result will display on the screen automatically.
  • Page 14 Double Beam UV Visible Spectrophotometer BSDBU-201-A Use numeric keypad to input wavelength (Fig 11). Fig 11 Press 【ENTER】 to change the wavelength from 656.1nm to 450.0nm,and then blank; After blanking, the screen displays as Fig 12. Fig 12 Load or delete data or curve (Take the “WL scan” test For example) Press 【3】...
  • Page 15 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 13 Fig 14 Table 1 Test File Type Quantitative Curve ***.fit Quantitative Test Result ***.qua WL Scan ***.wav Kinetics ***.kin DNA/Protein ***.dna Multi WL ***.mul WL Validity ***. wlv Accu. Validity ***.phv Save data or curve (Example: Save curve in “WL scan”) •...
  • Page 16 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 15 Table 2 representing representing representing 0,+,-,* ,/ 1,#,?,:,I 2,A,B,C,= 3,D,E,F,% 4,G,H,I,{ 5,J,K,L,} 6,M,N,O,~ 7,P,Q,R,S, 8,T,U,V,“ 9,W,X,Y,Z +/-/. -,., Print test report (For example: Print the report in “Basic mode”,Fig16) Press the key【PRINT】to print the report (curve or data you have loaded or tested, Fig 17).
  • Page 17 Double Beam UV Visible Spectrophotometer BSDBU-201-A Before measurement • Make a blank reference solution by filling a clean cuvette (or test tube) half full with distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid.
  • Page 18 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 20 1. Abs mode Push the blank cuvette into the Reference Light Path and Main Light Path. Press 【F2 】 to select Abs mode ,Press 【ZERO】for Blanking , and then Push the sample into Main Light Path to take reading(Fig 20) 2.
  • Page 19 Double Beam UV Visible Spectrophotometer BSDBU-201-A 4. Push the blank cuvette into the Reference Light Path and Main Light Path and press 【ZERO】for Blanking. There are now two choices for you to take: 4.1 Press【F3】to input known F value, Fig 23. Then push the sample into Main Light Path to take reading of concentration 4.2 Push sample of known concentration into the Main Light Path...
  • Page 20 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 25 Quantitative Press【2】in Main Menu for “Quantitative” Test (Fig 26). Press【ESC/STOP】to exit. Note: .If no automatic changer installed “cell #1” will disappear in Fig26. Fig 26 • How to operate 1. Press 【F1】to select unit of concentration (Fig 27).
  • Page 21 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 28 3. Press 【F2】in Fig 26 for more items to select .See Fig 29. Fig 29 3.1 Press 【F1】in Fig 29 to select fitting method. There are 4 methods for you to choose: Linear fit, linear fit through zero, square fit and cubic fit.
  • Page 22 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fitting Method Fitting Equation constants linear fit through C=K1×A K1, r* zero Linear fit C=K0+K1×A K0,K1,r* square fit C=K0+K1×A+K2×A K0,K1,K2 cubic fit C=K0+K1×A+K2×A +K3×A K0,K1,K2,K3 * r : regression co-efficients, default=1 3.3 Press 【F3】in Fig 29 to establish a standard curve by measuring a group of standard samples.
  • Page 23 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 32 3.3.3 Pull the first sample cuvette of known concentration into the light path, Press the key【START】to get values of standard curve one by one (Fig 33). 3.3.4 Press【F4】to draw the curve. You can get a different curve by pressing【F1 】...
  • Page 24 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 35 square fit WL : 6 56 .1 n m Ab s : 1 2 : 3 5 : 2 7 Cell -1 .0 Ab s 4 .0 Pr es s (ESC) to r et u rn . . .
  • Page 25 Double Beam UV Visible Spectrophotometer BSDBU-201-A established using the method shown in 3.3. Note: All sample results must be taken in screen Fig26. 4.1 Push the blank cuvette into the Reference Light Path and Main Light Path and press 【ZERO】for blanking.
  • Page 26 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 41 • Scan sample 1. Press 【F1 】 to setup, input the start wavelength, and end wavelength by pressing the numeric keypad (Fig 42). Note: This instrument scans from high to low wavelength. Browse and select the items of scan step and scan speed by 【...
  • Page 27 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 43 3. Put the blank cuvette into the Reference Light Path and Main Light Path, press 【ZERO 】 to scan the base line (Fig 44). Press the key 【ESC/STOP】to stop scanning; Fig 44 4.
  • Page 28 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 46 5. If you want to change the scale, press 【＜】 or 【＞】 to change “x” scale (Fig 47), input upper limit and lower limit by pressing the numeric keypad . To change 【...
  • Page 29 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 49 1. Peak to peak, press 【F1 】 to set “peak height” and input value by pressing 【 】 to search the peak from left to right and the numeric keypad (Fig 50). Press ∧...
  • Page 30 Double Beam UV Visible Spectrophotometer BSDBU-201-A Press 【PRINT】to print the curve you have loaded or scanned (Fig 52). Note: The report always is printed in Fig 46 Fig 52 Kinetics Press【4】in main menu for “Kinetics” (Fig 53). 【ESC/STOP】to exit. To load a previous kinetics result, press【LOAD 】 and select a previously stored result (.kin)
  • Page 31 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 53 • Test 1. Press 【F1 】 to set “Total Time”, ”Delay Time”, ”Time interval”, and input the value by pressing the numeric keypad (Fig 54). Fig 54 Select the test mode (“Abs” or “%T”) by pressing【F2】(Fig 55).
  • Page 32 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 56 5. Press 【F3】to process the data, and enter “Begin Time”, ”End Time” and ”Factor” (Fig 57) and the value in I.U. will be calculated and displayed (Fig 58). The average straight line between the Begin Time and End Time will be calculated.
  • Page 33 Double Beam UV Visible Spectrophotometer BSDBU-201-A scan”. • Save Curve Press the key 【SAVE 】 to save curve. Note: Load/Save requires the first kinetics display page Fig. 56. Press ESC if in Search to return to the required page. • Print Test Report Press the key【PRINT】to print the curve you have loaded or scanned (Fig 59).
  • Page 34 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 60 To load previous DNA results, press (LOAD) and select a previously stored result (.dna) • Test 1. To use a simpler or different algorithm, you can enter your own values for f1-f4.
  • Page 35 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 63 Press 【F3】to select the unit of concentration (Fig 64). Fig 64 4. Push the blank cuvette into the Reference Light Path and Main Light Path , then press 【ZERO】for blanking . 5. Pull the sample cuvette into Main Light Path, press 【START 】 to test the sample.
  • Page 36 Double Beam UV Visible Spectrophotometer BSDBU-201-A 6. If there is more than one sample, repeat step 5 for the next sample. 7. Press the key 【＜)or【＞】for searching. Input the sample number (Fig 66), 【 】or ∨ 【 】to browse the result will be displayed on the screen. Press the key ∧...
  • Page 37 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 68 To load previous Multi Wavelength results, press (LOAD) and select previously stored results (.mul) • Test 1. Press 【F1】to setup a group of wavelengths for testing by pressing the numeric keypad followed by【ENTER】. (٨) or (٧) to modify the inputted data Fig.
  • Page 38 Double Beam UV Visible Spectrophotometer BSDBU-201-A 3. Push the blank cuvette into the Reference Light Path and Main Light Path, then press 【ZERO】for Blanking . 4. Pull the sample cuvette into Main Light Path, press【START 】 to test. The test results will be displayed on the screen (Fig 71).
  • Page 39 Double Beam UV Visible Spectrophotometer BSDBU-201-A Setting and Calibration Utility Press【7】in Main menu for “Utility” (Fig 73). 【ESC/STOP】to exit. Fig 73 WL Reset Press【1】to reset wavelength (Fig74). Fig 74 Printer Press【2】to set printer (Fig 75). 【ESC/STOP】to exit. Fig 73 www.biolabscientific.com...
  • Page 40 Double Beam UV Visible Spectrophotometer BSDBU-201-A 1. Press【1】in Fig 75 to Reset Printer. 2. Press【2】in Fig 75 to select print port (LPT or Comm., Fig 76). Fig 76 Press【3 】 in Fig 75 to select printer (HP PCL (1 colour cartridge), PCL (black mode), Epson ESC/P or Epson/P2 or above, Fig77).
  • Page 41 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig78 Lamp Press【3】to set lamp (Fig 79). 【ESC/STOP】to exit. Fig 79 Press【1】in Fig 79 to switch on/off D2. Fig 80. Fig 80 www.biolabscientific.com...
  • Page 42 Double Beam UV Visible Spectrophotometer BSDBU-201-A 【 】or ∨ 【 】to 2. Press【2】in Fig 79 to reset usage time of D2(Fig 81). Press ∧ select “Yes” or “No”, and then press 【ENTER】. Fig 81 Press【3】in Fig 79 to switch on/off W. The indication is also on the top right corner of the screen (Fig 82).
  • Page 43 Double Beam UV Visible Spectrophotometer BSDBU-201-A Press【5 】 in Fig 79 to set the switch usage point of D2 and W lamp (Fig 84). Fig 84 • Clock Press 【4】In Fig73 to set the display mode and modify the clock (Fig 85).
  • Page 44 Double Beam UV Visible Spectrophotometer BSDBU-201-A 2. Press 【2】in Fig 85 to modify date by pressing the numeric keypad. 3. Press【3】in Fig 85 to set the date display on the top right corner of the screen. 4. Press【4】in Fig 85 to set the time display on the top right corner of the screen (Fig 87).
  • Page 45 Double Beam UV Visible Spectrophotometer BSDBU-201-A Press 【SETλ 】 to set the wavelength. Press 【ENTER】to edit and input wavelength by pressing the numeric keypad (Fig 90). 【ESC/STOP】to finish inputting and exit. Fig 90 Press【F1】to set the standard value, Press 【ENTER】to edit and input by pressing the numeric keypad (Fig 91).
  • Page 46 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 93 5. Press【ZERO】for Blanking. 6. Put the sample (calibrated neutral density filter) into Main Light Path. Press 【START】to check. The results will be displayed on the screen (Fig 94). If the discrepancy between the results and the calibrated standards is not more than the tolerance, “pass”...
  • Page 47 Double Beam UV Visible Spectrophotometer BSDBU-201-A wavelength by pressing the numeric keypad (Fig96). 【 ESC/STOP 】 to finish inputting and exit. Fig 96 2. Press 【F2】to select test mode (Abs or %T, Fig 97). Fig 97 3. Press 【 F3 】 to set tolerance (Fig 98). Input the value by pressing the numeric keypad.
  • Page 48 Double Beam UV Visible Spectrophotometer BSDBU-201-A more than the tolerance, “pass” will be displayed after the test results. Otherwise, “fail” will be displayed. Fig 99 6.The result can be saved, loaded and printed by pressing【SAVE】, 【LOAD】and 【PRINT】  Connect to PC Press【8】in Fig 73 to connect to PC (Fig 100).

  • Page 49: Instrument Maintenance

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Press【9】in Fig 73 to turn on/off the beeper  Delete entire saved files Press【F1】in Fig 73 to delete entire saved files. After the delete the files, double confirm need to do.  Restore default Press【F2】in Fig 73 to restore the default parameters.

  • Page 50: Troubleshooting

    Double Beam UV Visible Spectrophotometer BSDBU-201-A Troubleshooting 1, No response after power on Possible Cause Solution  Bad contact in power supply Improve the contact  Fuse melt Replace a new fuse 2, No Stable Reading Possible Cause Solution  No enough pre-warm Increase the pre-warm time ...
  • Page 51 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 101 Step 4: Replace a new Fuse Pick out the Spare Fuse and replace it to the working position. Step 5: Reset the Fuse Seat Replace the Fuse Seat in the power socket...
  • Page 52 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig 103 Step 5: Replace Lamps Fig 104 Top View of Lamp Chamber 1) Replace D2 Lamp Unscrew the 2 screws on the D2 Flange (Indicated in the Red Circles in Fig 105), unplug the power connector（Indicated in the Red Square in Fig.104）in the Power Board and remove the D2 lamp.
  • Page 53 Double Beam UV Visible Spectrophotometer BSDBU-201-A Fig. 105 2) Replace W lamp Remember the direction of the filament before pull out the W lamp. Be sure that the new lamp’s filament is in the same direction as before. Pull out the defected W lamp and draw on the Cotton Glove. Insert the new W lamp as deep as possible on the Lamp Seat.
  • Page 54 Double Beam UV Visible Spectrophotometer BSDBU-201-A Switch on the power, observe the Entrance Facula, and it should focus on the center of the Slit. (Fig.107) If the Facula deviate to Left or Right, then loosen the No.2 screws in Fig.107 and move the lamp seat to Left or Right until it focus on the center of the slot.
  • Page 55 Double Beam UV Visible Spectrophotometer BSDBU-201-A Appendix A DNA/Protein Test Algorithm Test Name Method Wavelengt Calculations Parame Displayed h(s) ters Units DNA MEASUREMENT Absorbance =62.9 DNA: 260nm DNA/Protein difference concentration: =36.0 μg/ml 280nm (260,280) =155 Protein:μg 320nm Concentrati Protein (optional)
  • Page 56 Double Beam UV Visible Spectrophotometer BSDBU-201-A at the analytical wavelength, as shown in Figure A1.The residual absorbance is the true absorbance of the analyte. This technique is less reliable when the spectra of the analyte and of the interferent are highly similar. Moreover, it can correct for only one interference Fig A1 Isoabsorbance correction A.2 Three-point correction...
  • Page 57 Double Beam UV Visible Spectrophotometer BSDBU-201-A BIOLAB SCIENTIFIC LTD. 3660 Midland Avenue, Suite 300, Toronto, Ontario M1V 0B8 Canada Email: contact@biolabscientific.com Tel: +1 707 533 1445 Website: www.biolabscientific.com www.biolabscientific.com...

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