BioLAB BSDBU-201-B Operation Manual
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BioLAB BSDBU-201-B Operation Manual

Double beam uv visible spectrophotometer
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Operation
Manual
BSDBU-201-B
Double Beam UV Visible Spectrophotometer
Thank you for Choosing Biolab products. Please read the "Operating Instructions" and
"Warranty" before operating this unit to assure proper operation.

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Summary of Contents for BioLAB BSDBU-201-B

  • Page 1 Operation Manual BSDBU-201-B Double Beam UV Visible Spectrophotometer Thank you for Choosing Biolab products. Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper operation.

  • Page 2: Table Of Contents

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Index Safety ……………………………………………………………………...2 General ………………………………………………………………………..2 Electrical ……………………………………………………………………..2 Warning ………………………………………………………………………..3 Performance ..…………………………………………………………..3 Radio Interference …………………………………………………………..3 Introduction …………………………………………………………………..5 Working Principle …………………………………………………………..6 Unpacking Instructions ……………………………………………………..7 Specifications ……………………………………………..…………………..8 Installation ……………………………………………………………………..8 Operation ……………………………………………………..……….……..9 Prepare the Spectrophotometer …………………………………………..6 Analyse Sample ……………………………………………………………..

  • Page 3: Safety

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Safety The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. This instrument should be operated by appropriate laboratory technicians.

  • Page 4: Warning

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Warning Any interruption of the protective conductor inside or outside the apparatus or disconnection of the protective earth terminal is likely to make the apparatus dangerous. Intentional interruption is prohibited. Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation.

  • Page 5: Introduction

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Introduction This instrument (Fig 1) is a double beam, general purpose instrument designed to meet the needs of the Conventional Laboratory, This instrument is ideal for various applications, such as: Chemistry, Biochemistry, petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control and research.

  • Page 6: Working Principle

    Double Beam UV Visible Spectrophotometer BSDBU-201-B The Sample Compartment Working Principle The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps to supply the light; 2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation;...

  • Page 7: Unpacking Instructions

    Double Beam UV Visible Spectrophotometer BSDBU-201-B 3) A sample compartment to accommodate the sample solution; 4) Two detectors to receive the transmitted light and convert it to an electrical signal; and 5) A digital display to indicate absorbance or transmittance.

  • Page 8: Specifications

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Note: The printer and auto-cell holder mentioned in this manual are all optional accessories, they do not come standard with the instrument. Specifications: • Wavelength Range: 190-1100nm • Spectral Bandpass: 0.5/1/2/4nm • Wavelength Accuracy: ±0.3nm...

  • Page 9: Operation

    Double Beam UV Visible Spectrophotometer BSDBU-201-B 5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes before taking any readings. We suggest you then do the Calibrate System with the Search 656.1nm to set the wavelength to the deuterium lamp emission line.
  • Page 10 Double Beam UV Visible Spectrophotometer BSDBU-201-B • Description of keys LOAD Load data or curve saved before; 【 】 SAVE Save data or curve; 【 】 SETλ Set wavelength; 【 】 ZERO Blank or scan the user base line; 【...
  • Page 11 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 4 Fig 4A Fig 5 www.biolabscientific.com...
  • Page 12 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 6 Fig 7 • Basic operation Set Slit The default value of the Slit Width is 2.0nm, if you want to choose other Slit Width, press 【F1 】 first, then press arrow key to choose the one you want and press 【ENTER 】 to confirm.
  • Page 13 Double Beam UV Visible Spectrophotometer BSDBU-201-B Note 1. If the reference solution is too thick, “Energy Low…”will appear following the “Blanking…”on the screen (Fig 8).If “Energy too Low…” appears following the “Blanking…”,the test will be paused and “Warning…”will appear on the screen.(Fig 9) 2.
  • Page 14 Double Beam UV Visible Spectrophotometer BSDBU-201-B reference solution into the Reference Light Path and the sample cuvette with reference solution into the Sample Light Path, Press 【ZERO】to obtain the user baseline. Then take out the sample cuvette, replace the reference solution with sample solution after flushing the cuvette completely.
  • Page 15 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 12 ※ Load or delete data or curve (Take the “WL scan” test For example) Press 【3 】 in Fig.7 go into “WL scan”. After【LOAD】being pressed, the first file (ABC.wav)in memory will appear on the bottom line of screen .Showed as Fig 13. Press 【 】...
  • Page 16 Double Beam UV Visible Spectrophotometer BSDBU-201-B Table 1 Test File Type Quantitative Curve ***.fit Quantitative Test Result ***.qua WL Scan ***.wav Kinetics ***.kin DNA/Protein ***.dna Multi WL ***.mul WL Validity ***. wlv Accu. Validity ***.phv Save data or curve (Example: Save curve in “WL scan”) •...
  • Page 17 Double Beam UV Visible Spectrophotometer BSDBU-201-B Table 2 representing representing representing 0,+,-,* ,/ 1,#,?,:,I 2,A,B,C,= 3,D,E,F,% 4,G,H,I,{ 5,J,K,L,} 6,M,N,O,~ 7,P,Q,R,S, 8,T,U,V,“ 9,W,X,Y,Z +/-/. -,., Print test report (For example: Print the report in “Basic mode”,Fig16) Press the key【PRINT】to print the report (curve or data you have loaded or tested, Fig 17).

  • Page 18: Analyse Sample

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Analyze Sample For different user’s requirements, we provide different test methods. Basic Mode Push the blank cuvette into the Reference Light Path and Main Light Path. In main menu (Fig7),press【1 】 to enter “Basic mode” test . After automatically blanking, it will display as Fig 18 (automatic changer installed) or Fig 19 ( automatic changer uninstalled) and wait for the operator.
  • Page 19 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 20 1. Abs mode Push the blank cuvette into the Reference Light Path and Main Light Path. Press 【F2 】 to select Abs mode ,Press 【ZERO】for Blanking , and then Push the sample into Main Light Path to take reading(Fig 20) 2.
  • Page 20 Double Beam UV Visible Spectrophotometer BSDBU-201-B 4. Push the blank cuvette into the Reference Light Path and Main Light Path and press 【ZERO】for Blanking. There are now two choices for you to take: 4.1 Press【F3】to input known F value, Fig 23. Then push the sample into Main Light Path to take reading of concentration 4.2 Push sample of known concentration into the Main Light Path...
  • Page 21 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 25 Quantitative Press【2】in Main Menu for “Quantitative” Test (Fig 26). Press【ESC/STOP】to exit. Note: .If no automatic changer installed “cell #1” will disappear in Fig26. Fig 26 • How to operate 1. Press 【F1】to select unit of concentration (Fig 27).
  • Page 22 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 28 3. Press 【F2】in Fig 26 for more items to select .See Fig 29. Fig 29 3.1 Press 【F1】in Fig 29 to select fitting method. There are 4 methods for you to choose: Linear fit, linear fit through zero, square fit and cubic fit.
  • Page 23 Double Beam UV Visible Spectrophotometer BSDBU-201-B Square fit C=K0+K1×A+K2×A K0,K1,K2 Cubic fit C=K0+K1×A+K2×A +K3×A K0,K1,K2,K3 * r : regression co-efficients, default=1 3.3 Press 【F3】in Fig 29 to establish a standard curve by measuring a group of standard samples. See Fig 30.
  • Page 24 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 32 3.3.3 Pull the first sample cuvette of known concentration into the light path, Press the key【START】to get values of standard curve one by one (Fig 33). 3.3.4 Press【F4】to draw the curve. You can get a different curve by pressing【F1 】 to select a different fitting method.
  • Page 25 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 35 Square fit WL : 65 6 .1 n m Abs : 1 2 : 3 5 : 2 7 Cell -1 .0 Ab s 4 .0 Pres s (E SC) to retu r n . . .
  • Page 26 Double Beam UV Visible Spectrophotometer BSDBU-201-B Note: All sample results must be taken in screen Fig 26. 4.1 Push the blank cuvette into the Reference Light Path and Main Light Path and press 【ZERO】for blanking. 4.2 Pull the sample cuvette into Main Light Path, press the key【START】, the results will be displayed on the screen (Fig 38).
  • Page 27 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 41 • Scan sample 1. Press 【F1 】 to setup, input the start wavelength, and end wavelength by pressing the numeric keypad (Fig 42). Note: This instrument scans from high to low wavelength. Browse and select the items of scan step and scan speed by pressing 【...
  • Page 28 Double Beam UV Visible Spectrophotometer BSDBU-201-B scan the base line (Fig 44). Press the key 【ESC/STOP】to stop scanning; Fig 44 4. Put the sample cuvette into Main Light Path, press 【START】to scan the sample(Fig 45) 【ESC/STOP 】 to stop scanning. When scan has finished the beeper beeps 3 times (Fig 46).
  • Page 29 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 47 Fig 48 6. Press 【F3 】 to search the Abs/%T value of the scan. There are two ways for you to search (Fig 49). Fig 49 1. Peak to peak, press 【F1 】 to set “peak height” and input value by pressing the numeric keypad (Fig 50).
  • Page 30 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 50 Fig 51 2. Point to point, Press 【＞】 to search the point from left to right and press 【＜) to search from right to left. The search step interval is the same as the scan step. The value of every point searched will be displayed on the screen.
  • Page 31 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 52 Kinetics Press【4】in main menu for “Kinetics” (Fig 53). 【ESC/STOP】to exit. To load a previous kinetics result, press【LOAD 】 and select a previously stored result (.kin) www.biolabscientific.com...
  • Page 32 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 53 • Test 1. Press 【F1 】 to set “Total Time”, ”Delay Time”, ”Time interval”, and input the value by pressing the numeric keypad (Fig 54). Fig 54 2. Select the test mode (“Abs” or “%T”) by pressing【F2】(Fig 55).
  • Page 33 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 56 5. Press 【F3】to process the data, and enter “Begin Time”, ”End Time” and ”Factor” (Fig 57) and the value in I.U. will be calculated and displayed (Fig 58). The average straight line between the Begin Time and End Time will be calculated.
  • Page 34 Double Beam UV Visible Spectrophotometer BSDBU-201-B • Save Curve Press the key 【SAVE 】 to save curve. Note: Load/Save requires the first kinetics display page Fig. 56. Press ESC if in Search to return to the required page. • Print Test Report Press the key【PRINT】to print the curve you have loaded or scanned (Fig 59).
  • Page 35 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 60 To load previous DNA results, press (LOAD) and select a previously stored result (.dna) • Test 1. To use a simpler or different algorithm, you can enter your own values for f1-f4.
  • Page 36 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 63 3. Press 【F3】to select the unit of concentration (Fig 64). Fig 64 4. Push the blank cuvette into the Reference Light Path and Main Light Path , then press 【ZERO】for blanking .
  • Page 37 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 66 • Recall the default Press the key【F4】to recall the default of the f1-f4. • Save Data Press the key 【SAVE 】 to save data. • Print Test Report Press the key【PRINT】to print the test result (Fig 67).
  • Page 38 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 68 To load previous Multi Wavelength results, press (LOAD) and select previously stored results (.mul) • Test 1. Press 【F1】to setup a group of wavelengths for testing by pressing the numeric keypad followed by【ENTER】. (٨) or (٧) to modify the inputted data Fig. 69. Press【ESC/STOP】to finish setup and exit.
  • Page 39 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 71 5. If there is more than one sample, repeat step 4 for the next sample. Note: When the test has finished, the wavelength will go to the first WL. 6. Press 【＜)or【＞】for searching. Input the sample number, the result will be displayed on the screen.

  • Page 40: Setting And Calibration

    Double Beam UV Visible Spectrophotometer BSDBU-201-B Setting and Calibration Utility Press in Main menu for “Utility” (Fig 73). ESC/STOP to exit. 【 】 【 】 Fig 73 • WL Reset Press【1】to reset wavelength (Fig74). Fig 74 • Printer Press【2】to set printer (Fig 75). 【ESC/STOP】to exit.
  • Page 41 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 75 1. Press【1】in Fig 75 to Reset Printer. 2. Press【2】in Fig 75 to select print port (LPT or Comm., Fig 76). Fig 76 3. Press【3 】 in Fig 75 to select printer (HP PCL (1 colour cartridge), PCL (black mode), Epson ESC/P or Epson/P2 or above, Fig77).
  • Page 42 Double Beam UV Visible Spectrophotometer BSDBU-201-B 4. Press【4】in Fig 75 to select print mode. If you select “Print screen” mode, a little icon will be displayed on the top line of the screen (Fig 78), if you select “Print report” mode, the little icon will disappear.
  • Page 43 Double Beam UV Visible Spectrophotometer BSDBU-201-B 2. Press【2】in Fig 79 to reset usage time of D2(Fig 81). Press 【 】 ∧ or【 】 ∨ to select “Yes” or “No”, and then press 【ENTER】. Fig 81 3. Press【3】in Fig 79 to switch on/off W. The indication is also on the top right corner of the screen (Fig 82).
  • Page 44 Double Beam UV Visible Spectrophotometer BSDBU-201-B 5. Press【5 】 in Fig 79 to set the switch usage point of D2 and W lamp (Fig 84). Fig 84 • Clock Press 【4】In Fig73 to set the display mode and modify the clock (Fig 85). 【ESC/STOP】to exit.
  • Page 45 Double Beam UV Visible Spectrophotometer BSDBU-201-B 2. Press 【2】in Fig 85 to modify date by pressing the numeric keypad. 3. Press【3】in Fig 85 to set the date display on the top right corner of the screen. 4. Press【4】in Fig 85 to set the time display on the top right corner of the screen (Fig 87).
  • Page 46 Double Beam UV Visible Spectrophotometer BSDBU-201-B 1. Press 【SETλ 】 to set the wavelength. Press 【ENTER】to edit and input wavelength by pressing the numeric keypad (Fig 90). 【ESC/STOP】to finish inputting and exit. Fig 90 2. Press【F1】to set the standard value, Press 【ENTER】to edit and input by pressing the numeric keypad (Fig 91).
  • Page 47 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 93 5. Press【ZERO】for Blanking. 6. Put the sample (calibrated neutral density filter) into Main Light Path. Press 【START】to check. The results will be displayed on the screen (Fig 94). If the discrepancy between the results and the calibrated standards is not more than the tolerance, “pass”...
  • Page 48 Double Beam UV Visible Spectrophotometer BSDBU-201-B 1. Press 【F1】to set the standard peak. Press 【ENTER】to edit and input wavelength by pressing the numeric keypad (Fig96). 【ESC/STOP】to finish inputting and exit. Fig 96 2. Press 【F2】to select test mode (Abs or %T, Fig 97).
  • Page 49 Double Beam UV Visible Spectrophotometer BSDBU-201-B results. Otherwise, “fail” will be displayed. Fig 99 6.The result can be saved,loaded and printed by pressing【SAVE】,【LOAD】and 【PRINT】 • Connect to PC Press【8】in Fig 73 to connect to PC (Fig 100). If the instrument is controlled by PC, the screen displays as Fig 100A.

  • Page 50: Instrument Maintenance

    Double Beam UV Visible Spectrophotometer BSDBU-201-B • Delete entire saved files Press【F1】in Fig 73 to delete entire saved files. After the delete the files, double confirm need to do. • Restore default Press【F2】in Fig 73 to restore the default parameters.

  • Page 51: Spare Parts Replacement

    Double Beam UV Visible Spectrophotometer BSDBU-201-B 2.No Stable Reading Possible Cause Solution • No enough pre-warm Increase the pre-warm time • Glass cuvettes used in UV Range Use Silicon Cuvettes in UV Range • unstable Sample Improve the sample • Much higher sample concentration Dilute the sample •...
  • Page 52 Double Beam UV Visible Spectrophotometer BSDBU-201-B Step 4: Replace a new Fuse Pick out the Spare Fuse and replace it to the working position. Step 5: Reset the Fuse Seat Replace the Fuse Seat in the power socket Step 6: Switch on the power Plug the socket and switch on the power 2.Replace lamps...
  • Page 53 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 103 Step 5: Replace Lamps Fig 104 Top View of Lamp Chamber 1) Replace D2 Lamp Unscrew the 2 screws on the D2 Flange (Indicated in the Red Circles in Fig 105), unplug the power connector（Indicated in the Red Square in Fig.104）in the Power Board and remove...
  • Page 54 Double Beam UV Visible Spectrophotometer BSDBU-201-B Fig 105 2) Replace W lamp Remember the direction of the filament before pull out the W lamp. Be sure that the new lamp’s filament is in the same direction as before. Pull out the defected W lamp and draw on the Cotton Glove. Insert the new W lamp as deep as possible on the Lamp Seat.
  • Page 55 Double Beam UV Visible Spectrophotometer BSDBU-201-B If the facula deviate to Up and Down, then loosen the No.1 screws in Fig.107 and move the lamp seat Up and Down until the facula focus on the center of the slit. Then fix the No. 1 screws again.
  • Page 56 Double Beam UV Visible Spectrophotometer BSDBU-201-B Appendix A DNA/Protein Test Algorithm Test Name Method Wavelength Calculations Parameters Displayed Units DNA MEASUREMENT DNA/Protein Absorbance =62.9 DNA: 260nm difference concentration: =36.0 μg/ml 280nm Concentration (260,280) =1552 Protein:μg/ 320nm Protein =757.3 (optional) concentration...
  • Page 57 Double Beam UV Visible Spectrophotometer BSDBU-201-B selecting a reference wavelength at which the interfering compound exhibits the same absorbance as it does at the analytical wavelength. The absorbance at this reference wavelength is subtracted from the absorbance at the analytical wavelength, as shown in Figure A1.The residual absorbance is the true absorbance of the analyte.
  • Page 58 Double Beam UV Visible Spectrophotometer BSDBU-201-B BIOLAB SCIENTIFIC LTD. 3660 Midland Avenue, Suite 300, Toronto, Ontario M1V 0B8 Canada Email: contact@biolabscientific.com Tel: +1 707 533 1445 Website: www.biolabscientific.com www.biolabscientific.com...

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