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Sherwood Scientific 410 Industrial Operator's Manual

Flame photometer
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Model 410 Industrial
&
Model 410 Clinical
Flame Photometer
Operator
Manual
Copyright 2012 Sherwood Scientific Ltd.
All rights reserved.
th
410 91 001
Issue 7
24
March 2012
ECN 736
G:\DOCUMENT CONTROL\Manuals\Flame\410\41091001 M410 & 410C with Interface Operator Manual Issue 7.doc

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Summary of Contents for Sherwood Scientific 410 Industrial

  • Page 1 Model 410 Industrial & Model 410 Clinical Flame Photometer Operator Manual Copyright 2012 Sherwood Scientific Ltd. All rights reserved. 410 91 001 Issue 7 March 2012 ECN 736 G:\DOCUMENT CONTROL\Manuals\Flame\410\41091001 M410 & 410C with Interface Operator Manual Issue 7.doc...

  • Page 2: Intended Use

    The information contained in this manual was correct at the time of going to print. However, Sherwood Scientific’s policy is one of continuous product improvement and the right to change specifications, equipment and maintenance procedures at any time, without notice, is reserved.

  • Page 3: Table Of Contents

    Model 410 & 410C Flame Photometer with Interface Contents Page Intended Use ............................ 2 1 Introduction ..........................6 Introduction ......................6 Summary of the Test ....................6 Principles of Operation .................... 7 Reagents........................7 Dilutions……………………………………………………………………………...7 Storage……………………………………………………………………………..…7 Purification ......................7 2 Installation ...........................
  • Page 4 Model 410 & 410C Flame Photometer with Interface Contents Page 4 Operating Instructions ........................ 18 Front Panel Controls and Indicators (See Fig 5.1)........... 18 Power on ....................... 18 Flame on ....................... 18 Blank ........................18 Sensitivity Fine and Coarse ................... 18 Fuel ........................
  • Page 5 Model 410 & 410C Flame Photometer with Interface Contents Page 7 Troubleshooting ......................... 39 Power on LED not illuminated ................39 Flame on LED not illuminated ................39 General ......................... 39 Air Supply ......................39 Fuel Supply ......................39 Unable to set display to zero ..................

  • Page 6: Introduction

    Na, K, and Lithium (Li) in diluted clinical samples. An automatic sample dilutor, Model 805 is available. This manual chiefly refers to the Model 410 Industrial, but much is common to the Model 410 Clinical. For ‘Model 410’ please assume the text applies to both models unless specifically indicated that the information is relevant to the Model 410 Industrial only.

  • Page 7: Principles Of Operation

    Reagents Sherwood Scientific supplies a wide range of reagents, including standards, diluent and maintenance solutions, for use with the Model 410 Flame Photometer. Please refer to Section 8.3 and Appendix B for a complete list of the reagents.

  • Page 8: Installation

    NB The positioning of Gas cylinders should conform to National and local regulations. A supply of clean, dry, oil-free air at 1kg/cm² (14psig), flow rate 6 litres per minute. A suitable Sherwood Scientific air compressor is listed in Section 8.2. Waste Container A sink or waste container sited to the right of the instrument will ensure the minimum length of waste tubing.

  • Page 9: Site Conditions

    Model 410 & 410C Flame Photometer with Interface Installation continued Site Conditions WARNING Under no circumstances install the instrument beneath overhanging cupboards. There must be at least 1 metre of clear space above the chimney. For optimum performance, this instrument should be installed in accordance with the following conditions: - The environment must be clean and free from dust.

  • Page 10: Unpacking

    NOTE The Model 410 weighs 9.5kg, follow safe lifting techniques. Check all items for damage. Check that all the items on the Accessory List have been delivered. Contact your Sherwood Scientific distributor if you have any problems. The Model 410 is shipped with the following items: Accessory List CAT.

  • Page 11: Assembly

    Model 410 & 410C Flame Photometer with Interface Installation continued Assembly EQUIPMENT REQUIRED: - ‘Pozidriv’ (cross head) screwdriver 1PT; Flat blade screwdriver. To assemble the Model 410, proceed as follows: Lift off the chimney cap from the chimney assembly and lift out the glass chimney.
  • Page 12 Model 410 & 410C Flame Photometer with Interface Installation continued Assembly continued Connect the length of rubberised fuel tubing, (001 72 114) supplied, between the inlet connector on the Propane regulator, (item 5, Figure 4.2) and the cylinder regulator outlet connector. Secure with ’Unex’ tubing clamps, supplied. Ensure that the connector at the regulator inlet is tightened to 12nm torque.
  • Page 13 Model 410 & 410C Flame Photometer with Interface Installation continued Assembly continued Make sure that the constant head drain (Gas Trap) is positioned so that it is pushed fully down on its retaining screws. Use a wash bottle (item 1, Figure 2.4) to fill the ‘U’...

  • Page 14: Connecting A Printer Or Computer

    Model 410 & 410C Flame Photometer with Interface Installation continued Assembly continued Fit a suitable 3-pin plug to the a.c. supply lead. Check that the correct colour coded leads are connected to the plug terminals. Follow the plug manufacturer’s fitting instructions. CAUTION Ensure that the green/yellow earth connector in the a.c.

  • Page 15: Performance Characteristics And Specification

    Model 410 & 410C Flame Photometer with Interface Performance Characteristics and Specification Readout 3½-digit light emitting diode (LED) display, 12.5mm high. Display range 0 to 1999 (199.9 with decimal point on). NOTE If negative values are displayed the instrument is operating outside of the recommended measurement range.

  • Page 16: Warm Up

    Model 410 & 410C Flame Photometer with Interface Performance Characteristics and Specification continued Warm Up To achieve the stated specification the flame must be alight for a minimum of 30 minutes, with diluent being aspirated. Sample Requirement Type Dependent on application. However, samples should be water-based and not highly viscous nor non-homogeneous.

  • Page 17: 3.10 Power Requirements

    Model 410 & 410C Flame Photometer with Interface Performance Characteristics and Specification continued 3.10 Power Requirements Voltage 90V to 132V or 198V to 264V, 50/60Hz. Fuses Two 200mA slo-blo fuses are fitted for all voltage ranges. Power 20VA. 3.11 Fuel High-grade Propane, Butane or Propane/Butane mixture.

  • Page 18: Operating Instructions

    Model 410 & 410C Flame Photometer with Interface Operating Instructions Front Panel Controls and Indicators (See Fig 4.1). Power on (item 10) LED which illuminates when the instrument is switched on. Flame on (item 11) LED which illuminates when the flame is alight. Blank (item 5) This control is used to set the display to zero while aspirating a blank solution.
  • Page 19 Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Front Panel Controls and Indicators continued Figure 4.1 Front Panel 1. Filter selector. 2. Digital display. 3. Inspection flap. 4. Fuel adjustment control. 5. Blank control. 6. Coarse sensitivity control. 7. Fine sensitivity control. 8.

  • Page 20: Printer/Computer Connection

    Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Front Panel Controls and Indicators (See Fig 4.1.1) continued Printer/Computer Connector (item 1) Four pin socket for connecting the instrument to a serial printer or computer. Selector Button (item 2) Each press of the select button will cause the buzzer to sound and the next LED in the sequence B, C, S, R to illuminate.
  • Page 21 Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Front Panel Controls and Indicators continued Figure 4.1.1 Interface Panel 1. Printer/Computer connector. 2.Selector button. 3.Print button. 4.Lineariser LED. 5. Blank LED. 6. Calibration LED. 7. Sample LED. 8. Repeat LED. 9. Print LED. 410 91 001 Issue 7 March 2012...
  • Page 22 Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Rear Panel Controls and Connectors Figure 4.2 Rear Panel 1. Power connector and voltage selector. 2. Gas inlet connector. 3. Data output sockets. 4. Air inlet connector. 5. Air pressure gauge and air regulator adjuster. 6.

  • Page 23: Power

    Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Rear Panel Controls and Connectors (See Fig 4.2) continued IEC symbol advising user to refer to accompanying documentation. Serial Plate ~symbol denotes equipment suitable for alternating current only. Power (item 1) Three pin connector for the a.c.

  • Page 24: Initial Adjustment

    Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Initial Adjustment NOTE The following instructions assume that the air compressor is switched on and supplying the instrument with air. Check that the air pressure gauge (on the rear panel) indicates a reading between 11 and 13psig.

  • Page 25: Operating Instructions

    Operating Instructions NOTE For greatest accuracy and stability Sherwood Scientific recommend that a batch of diluent is made up from 1 part Diluent Concentrate and 999 parts deionised or good quality distilled water. Using diluent for setting blank and for dilution of samples and standards means that all solutions presented to the instrument contain the stability promoting ingredients contained in the diluent.
  • Page 26 Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Operating Instructions continued Figure 4.3 Typical Calibration Curve Check calibration standards and blank readings. Dilute the unknown solutions with diluent to give a concentration of the element under test within the range of the calibration standards. Several attempts might be necessary to determine the correct dilution ratio.

  • Page 27: Shutdown Procedure

    If this procedure is carried out regularly no difficulties should be experienced. Applications and Method Sheets are available from your Sherwood Scientific distributor or www.sherwood-scientific.com. A guide to Flame Photometry is available from your Sherwood Scientific Distributor. 410 91 001 Issue 7...

  • Page 28: Interface Operation

    Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Interface Operation With A Printer Connect the printer to the instrument with the cable provided and switch on the printer. Make sure that the printer is set to: - 9600 baud 8 bit No parity...

  • Page 29: Switching The Linearisation Function On And Off

    Model 410 & 410C Flame Photometer with Interface Operating Instructions continued Interface Operation continued With A Computer Connect the computer serial port to the instrument with the cable provided and switch on the computer. Open HyperTerminal on the computer (see Appendix C) and use the following for the communication settings: - 9600 baud 8 bit...

  • Page 30: Operational Precautions And Limitations

    Always use soap solution when checking for leaks in fuel or air lines. Do not allow fuel to flow in the presence of unguarded flames, e.g. cigarettes. Always use genuine Sherwood Scientific replacement parts. Do not, for example, replace the ‘U’ tube with one of different material, bore or length as this will cause a deterioration in the instrument’s performance.

  • Page 31: Maintenance

    Model 410 & 410C Flame Photometer with Interface Maintenance General Under the Daily, Weekly, Monthly and Six-Monthly Maintenance headings are summaries of the work and equipment required. The tasks are detailed from Section 6.6 onwards. For maintenance of the air compressor, dilutor and chart recorder, if used, refer to the instructions supplied with the equipment.

  • Page 32: Six-Monthly Maintenance

    If it is too low, continue with paragraph 6. If it is too high contact your Sherwood Scientific Distributor. Do not attempt to adjust the nebuliser, as the capillary position is fixed during manufacture, and is not adjustable.
  • Page 33 Model 410 & 410C Flame Photometer with Interface Maintenance continued Cleaning the Nebuliser continued Figure 6.1 Mixing Chamber 1. Nebuliser retainer. 2. Nebuliser. 3. End cap. 4. Fuel tubing. 5. Air tubing. 6. End cap securing screw. 7. ‘U’ tube. Fit a new length of nebuliser inlet tubing to the nebuliser.

  • Page 34: Cleaning The Mixing Chamber, Burner And Drain

    Model 410 & 410C Flame Photometer with Interface Maintenance continued Cleaning the Mixing Chamber, Burner and Drain EQUIPMENT REQUIRED Flat blade screwdriver, blade width 8 mm; Deproteinising Solution; Tissues. Check that the flame on LED is off, fuel is turned off at source, and the instrument and compressor are switched off.

  • Page 35: Cleaning The Optical Filters And Glass Chimney

    Model 410 & 410C Flame Photometer with Interface Maintenance continued Cleaning the Optical Filters and Glass Chimney EQUIPMENT REQUIRED Soft lint free cloth; Methanol; Cotton buds; Tissues; Cleaning Solution. Check that the flame on LED is off, fuel is turned off at source, and the 410 and compressor are switched off.
  • Page 36 Model 410 & 410C Flame Photometer with Interface Maintenance continued Cleaning the Optical Filters and Glass Chimney continued Figure 6.2 Filter Selector Soak the glass chimney in a 1 in 100 dilution of Cleaning Solution, agitating it periodically. Rinse the glass chimney thoroughly in deionised water and dry with clean tissues. Replace the glass chimney with the clear strip at the bottom.

  • Page 37: Voltage Selection And Fuse Replacement

    Model 410 & 410C Flame Photometer with Interface Maintenance continued Voltage Selection and Fuse Replacement EQUIPMENT REQUIRED Fuses. WARNING For continued protection against fire hazard use only the same type and rating of fuse that was fitted originally to the M410 refer to instrument rear panel.

  • Page 38: 6.10 Deproteinising Or Disinfecting Procedure

    Model 410 & 410C Flame Photometer with Interface Maintenance continued 6.10 Deproteinising or Disinfecting Procedure NOTE To deproteinise the system use Deproteinising Solution, to disinfect the system use Tubing Disinfectant. EQUIPMENT REQUIRED Deproteinising Solution or Tubing Disinfectant. Light the flame as detailed in Section 4.4. Present a beaker of Deproteinising Solution or Tubing Disinfectant to the nebuliser for 10 minutes.

  • Page 39: Troubleshooting

    Check the fuel control is set correctly, refer to Section 4.3. If the fuel restrictor (propane/butane only) is blocked, clean with the nebuliser cleaning wire, refer to Figure 2.1. If fault persists contact your Sherwood Scientific Distributor. 410 91 001...

  • Page 40: Unable To Set Display To Zero

    6.7. If using a chart recorder check that the data output connections are correct. If fault persists contact your Sherwood Scientific Distributor. Unable to set display to standard reading Check the concentration of the standard being aspirated, and that the dilution ratio of this standard is correct.

  • Page 41: Non-Linear Results

    A total of 10 numbers will be presented, after the last one is entered new values for slope and intercept will be displayed in HyperTerminal and the menu will reappear. Enter q to exit set-up mode. If fault persists contact your Sherwood Scientific Distributor. 410 91 001 Issue 7 March 2012...

  • Page 42: Print Led Flashing

    Make sure the Printer or Computer is switched on and the interface cable is securely connected, and then switch the instrument off and on again. If fault persists contact your Sherwood Scientific Distributor. Output Does Not Equal Display Perform Calibration as detailed below.

  • Page 43: Date & Time Not Reported Correctly

    Model 410 & 410C Flame Photometer with Interface Troubleshooting continued Date & Time Not Reported Correctly Set clock as detailed below With A Printer Connect the printer to the instrument with the cable provided and switch on the printer. Make sure that the printer is set to: - 9600 baud 8 bit No parity...

  • Page 44: With A Computer

    3 Set Clock (not Bluetooth) 4 or q to Quit Enter ‘3’ and follow the instructions:- Enter date and time in format. dd:mm:yy hh:mm:ss If fault persists contact your Sherwood Scientific Distributor. 410 91 001 Issue 7 March 2012 ECN 736...

  • Page 45: Spares And Accessories

    Model 410 & 410C Flame Photometer with Interface Spares and Accessories Ordering Information When ordering spares or accessories for your instrument, please give the following information to your Sherwood Scientific distributor. Instrument Serial No. Catalogue No. of Part (Cat. No.) Description Quantity required This will ensure that your order is dealt with quickly and efficiently.

  • Page 46: Standard Solutions

    Model 410 & 410C Flame Photometer with Interface Spares and Accessories continued Serial Number ITEM QUANTITY 001 08 234 Propane primary regulator, cylinder fixing 001 08 439 Butane primary regulator (182H) for 32lb Calor/Caravan cylinder 001 08 732 Butane primary regulator for ‘Gaz’ type cylinder 1 001 72 114 Fuel tubing per metre...

  • Page 47: Bibliography

    Model 410 & 410C Flame Photometer with Interface Appendix A Bibliography Reference Kolthoff, I.M., Z. Anal. Chem. 70 397 1927 Shohl, A.T., and Bennett, H.B. J. Biol. Chem. 78: 643 1928 Hald, P.M., J. Biol. Chem. 167. 499. 1947 Bernstein, R.E., S.Afr. J. Med. Sci. 17. 101, 1952 Alkemade, C.T.J., Smit J.

  • Page 48: M410 Clinical

    Model 410 & 410C Flame Photometer with Interface Appendix B Model 410 Clinical Addendum - Using the M410 Flame Photometer on Clinical Samples These sections are replacement sections to those in the Main M410 Instruction Manual and should be read in conjunction with it. Introduction The Sherwood Model 410 Clinical Flame Photometer can be used for clinical samples: - serum, plasma and urine.
  • Page 49 Model 410 & 410C Flame Photometer with Interface Appendix B continued Figure 2.7 1. Logo Plate 2. Lineariser Module Measurement Ranges for clinical samples Na serum 110 - 170mmol/l (diluted 1:200 ≡ 12.6 – 19.5ppm aspirated into flame) Na Urine 0 - 200mmol/l (diluted 1:200 ≡...
  • Page 50 Model 410 & 410C Flame Photometer with Interface Appendix B continued Specificity Na Range Errors in the Na readings due to Ca, K, Li presence in biological samples shall not be greater than 1mmol/l where the levels of those elements do not exceed 3.5mmol/l Ca, 100mmol/l K and 2.0mmol/l Li.
  • Page 51 Model 410 & 410C Flame Photometer with Interface Appendix B continued Sample requirement Serum plasma urine C.S.F. and sweat. Method of presentation: - The sample is either diluted using the following factors into a sample cup and is presented directly to the nebuliser inlet tube; or it is diluted using the Model 805 Dilutor, which is connected to the Model 410 via a weir cup assembly supplied with the Dilutor.
  • Page 52 A total of 4 numbers will be presented, after the last one is entered new values for slope and intercept will be displayed in HyperTerminal and the menu will reappear. Enter q to exit set-up mode. If fault persists contact your Sherwood Scientific Distributor. Spares and Accessories Serial Number Description...

  • Page 53: Hyperterminal

    Model 410 & 410C Flame Photometer with Interface Appendix C HyperTerminal This program is available in all versions of windows software. To access HyperTerminal and set it up to communicate with your new module:- Select All Programs, Accessories, Communications HyperTerminal New Connection Name (e.g.

  • Page 54: Installing A Natural Gas Regulator Kit

    Model 410 & 410C Flame Photometer with Interface Appendix D Installing a Natural Gas Regulator Kit Remove the fuel restrictor (item 5, Figure 2.1) and connect the fuel tubing (item 6, Figure 2.1) directly to the mixing chamber connection. Unscrew and remove the gas inlet, regulator inlet and regulator outlet connectors (items 4, 5 and 6, Figure 2.2) and remove the copper tubing (item 2, Figure 2.2).
  • Page 55 Model 410 & 410C Flame Photometer with Interface Appendix D continued Installing a Natural Gas Regulator Kit continued Locate the Natural gas regulator and mounting bracket ordered as optional accessories and packed separately from the instrument. Fit the mounting bracket to the rear panel with the two screws supplied and slot in the Natural gas regulator, Figure 2.3.

  • Page 56: Product Warranty

    Warranty Term: 12 Months Sherwood Scientific Ltd (Sherwood) warrants, subject to the conditions itemised within this document, through either Sherwood personnel or personnel of its authorised distributors, to repair or replace free of all charges, including labour, any part of this product which fails within the warranty time specified above, appertaining to this particular product.
  • Page 57 (including but not limited to the limitations of the liability of Sherwood as set forth above), shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Ltd. Except for the obligations specifically set forth in this warranty statement, in no event shall...

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