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Summary of Contents for Sherwood Scientific CHROMA M260
- Page 1 CHROMA M260 Colorimeter Operator Manual Copyright © 2010, Sherwood Scientific Ltd., All rights reserved. 260 91 000 Issue 1 May 2010 ECN 613 G:\DOCUMENT CONTROL\Manuals\Chroma\26091000 Operator Manual M260 Issue 1 #20864 on.doc...
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Page 2: Table Of Contents
1.1.1 If your Model 260 Colorimeter was supplied by a third party ..5 1.1.2 If the Instrument was obtained from Sherwood Scientific Ltd ..5 1.2 Using this Manual ..................6 1.3 Safety ......................6 1.4 Reagents ....................6 1.5 Sample Preparation .................. - Page 3 Sherwood Scientific Limited Chapter Five Product Description ..............11 5.1 Controls and Indicators ................11 5.1.1 Typical Front Panel Layout ............11 5.1.2 The LCD Display: ...............11 5.1.3 Buttons ..................11 5.1.4 Modifying Numbers etc..............12 5.2 Sample Heating and Incubation Times ............12 5.3 Operating Modes ..................13 5.3.1...
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Page 4: Intended Use
Sherwood Scientific Limited Intended Use This CHROMA Model 260 Colorimeter is intended to be used by professional chemists/biochemists. Programming the unit is easier with detailed understanding of the mechanisms of both the test kits to be used in conjunction with the instruments and the underlying principles of colorimetry. -
Page 5: Chapter One Introduction
Company where you purchased this unit: Company…………………………………………… Tel Number………………………………………… Contact Name………………………………………. Email……………………………………………….. 1.1.2 If the Instrument was obtained from Sherwood Scientific Ltd Contact for all issues of supply and operation should be sent to: info@sherwood-scientific.com Tel +44 (0)1223 243444 Fax +44 (0)1223 243300... -
Page 6: Using This Manual
Colorimeter to operate satisfactorily is dependent on the profile of the cuvette used. These are listed in Chapter 4 (see 4.1.1) of this manual. Warranty The Sherwood Scientific warranty statement is included at Appendix A. 260 91 000 Issue 1... -
Page 7: Chapter Two Installation
Sherwood Scientific Limited Chapter Two Installation Location The Colorimeter should be placed on a stable bench out of direct sunlight and with sufficient space to accommodate a separate heating block if necessary. Services Required The Model 260 Colorimeter is supplied with a universal power supply. -
Page 8: Thermostatted Cuvette Holder (267 86 001)
Sherwood Scientific Limited Connecting Peripheral Devices (continued) 2.5.2 Thermostatted Cuvette Holder (267 86 001) The thermostatted cuvette holder (if ordered) should be inserted with the power lead taken through the drain hole in the instrument before inserting the holder into the instrument. This device will, when connected to a power source, automatically settle to 37º... -
Page 9: Chapter Three Scientific Background
Sherwood Scientific Limited Chapter Three Scientific Background Historical We can trace the heritage of the Sherwood CHROMA range of colorimeters back to the very first Model 2 Colorimeter developed for a Dr Rose at Hammersmith Hospital in London by Evans Electro-Selenium Ltd. (EEL) in the 1940s. -
Page 10: Chapter Four Specifications
Sherwood Scientific Limited Chapter Four Specifications Sample Requirements When used with test kits, the manufacturer of the kit will specify the individual sample parameters depending on the particular assay. 4.1.1 Total Volume Required: Standard cuvettes: 1.4ml Part No: 471 88 300 Semi-micro cuvette: 600µl... -
Page 11: Chapter Five Product Description
Sherwood Scientific Limited Chapter Five Product Description Controls and Indicators 5.1.1 Typical Front Panel Layout 5.1.2 The LCD Display: Has three lines with a maximum of 16 characters per line. When results are displayed the digits are shown over two rows in double size. -
Page 12: Modifying Numbers Etc
Sherwood Scientific Limited Product Description (continued) 5.1.4 Modifying Numbers etc. Where there are numbers to change i.e. concentration factor values or letters to change, i.e. to label tests and to define sample numbers or names, then the Enter button changes the decade or letter position and the select buttons are used to change the value of the number or letter. -
Page 13: Operating Modes
Sherwood Scientific Limited Product Description (continued) Operating Modes 5.3.1 Warm Up There is a 15 minute warm up period built into the start up routine. This can be over-ridden by pressing the Enter button 5.3.2 Main Menu 5.3.2.1 Set-up While the Model 260 Colorimeter is warmed, you can enter Setup to: •... -
Page 14: Menu Structure
Sherwood Scientific Limited Menu Structure Main Menu Simple Absorbance % Transmission Factor Concentration Last Method Recall Edit Delete Print Concentration Factor Time Based Endpoint Kinetic Setup Time Format Date Format Set Clock Language NB. Uart is for RS232 parameter setup... -
Page 15: Chapter Six Operation
Sherwood Scientific Limited Chapter Six Operation Start Up Switch on the Colorimeter and, if needed, a heater block or water bath for sample and standard preparation. Allow the heater block, or water bath to get to the control temperature. NB The nominal temperature setting point of a Heating Block should be... -
Page 16: Simple Method
Sherwood Scientific Limited Operation (continued) Simple Method The simple method allows measurement of Absorbance or % Transmission of a sample. Here there is no calibration procedure. The instrument is zeroed against a blank solution before a reading is taken of the sample. Put the blank cuvette in the cuvette holder and press the zero button when prompted. -
Page 17: Concentration Methods
Sherwood Scientific Limited Operation (continued) Concentration Methods As stated, the majority of applications for the Colorimeter will be the measurement of the concentration of an analyte either by:- 1. Using a factor supplied by the test kit manufacturer. 2. Comparison with a calibrator (or “standard”) of known concentration. -
Page 18: Incubation And Measurement Times
Sherwood Scientific Limited Operation (continued) Consequently, in the Model 260 Colorimeter, four method types can be entered, edited and/or run to accommodate the methodologies outlined above. They are respectively denoted as:- End Point Method with Factor End Point Method with Calibrator... -
Page 19: Chapter Seven Method Setup
Sherwood Scientific Limited Chapter Seven Method Set-up The following flowcharts give detailed illustration of entering a new Method of a type accommodated by the Model 260 Colorimeter software (outlined in Section 6.4 and 6.5). Factor Method – Set-up Procedure Select Method from Main Menu using >> then press ↵ ↵ ↵ ↵... -
Page 20: Concentration Method - Setup Procedure
Sherwood Scientific Limited Concentration Method – Set-up Procedure Select Method from Main Menu using >> then press ↵ ↵ ↵ ↵ Select New from Method Menu using >> then press ↵ ↵ ↵ ↵ Select Concentration from Mode Menu using >> then press ↵ ↵ ↵ ↵... -
Page 21: End Point Method With Factor - Setup Procedure
Sherwood Scientific Limited End Point Method with Factor – Set-up Procedure Select Method from Main Menu using >> then press ↵ ↵ ↵ ↵ Select New from Method Menu using >> then press ↵ ↵ ↵ ↵ Select Time Based from Mode Menu using >> then press ↵ ↵ ↵ ↵... -
Page 22: End Point Method With Calibrator - Setup Procedure
Sherwood Scientific Limited End Point Method with Calibrator – Set-up Procedure Select Method from Main Menu using >> then press ↵ ↵ ↵ ↵ Select New from Method Menu using >> then press ↵ ↵ ↵ ↵ Select Time Based from Mode Menu using >> then press ↵ ↵ ↵ ↵... -
Page 23: Kinetic Method With Factor Method - Setup Procedure
Sherwood Scientific Limited Kinetic Method with Factor Method – Set-up Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select New from Method Menu using >> then ↵ ↵ ↵ ↵ Select Time Based from Mode Menu using >> then ↵ ↵ ↵ ↵... -
Page 24: Kinetic Method With Calibrator - Setup Procedure
Sherwood Scientific Limited Kinetic Method with Calibrator – Set-up Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select New from Method Menu using >> then ↵ ↵ ↵ ↵ Select Time Based from Mode Menu using >> then ↵ ↵ ↵ ↵... -
Page 25: Chapter Eight Method Run Details
Sherwood Scientific Limited Chapter Eight Method Run Details The following flow charts give detailed illustration of running a Method of a type accommodated by the Model 260 Colorimeter, as outlined in Sections 6.4 and 6.5. Factor Method - Run Procedure Select Method from main menu using >>... -
Page 26: Concentration Method - Run Procedure
Sherwood Scientific Limited Concentration Method - Run Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select Recall from Method Menu using >> then ↵ ↵ ↵ ↵ Select run from Recall Menu using >> then ↵ ↵ ↵ ↵... -
Page 27: End Point Method With Factor - Run Procedure
Sherwood Scientific Limited End Point Method with Factor - Run Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select Recall from Method Menu using >> then ↵ ↵ ↵ ↵ Select Run from Recall Menu using >> then ↵ ↵ ↵ ↵... -
Page 28: End Point Method With Calibrator - Run Procedure
Sherwood Scientific Limited End Point Method with Calibrator - Run Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select Recall from Method Menu using >> then ↵ ↵ ↵ ↵ Select Run from Recall menu using >> then ↵ ↵ ↵ ↵... - Page 29 Sherwood Scientific Limited End Point Method with Calibrator - Run Procedure (continued) Enter the sample number using >> then ↵ ↵ ↵ ↵ for each position When prompted, put the sample blank and press the 0 The absorbance will be set to 0.000. Press enter↵ ↵ ↵ ↵...
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Page 30: Kinetic Method With Factor - Run Procedure
Sherwood Scientific Limited Kinetic Method with Factor – Run Procedure Select Method from Main menu using >> then ↵ ↵ ↵ ↵ Select Recall from Method Menu using >> then ↵ ↵ ↵ ↵ Select Run from Recall menu using >> then ↵ ↵ ↵ ↵... - Page 31 Sherwood Scientific Limited Kinetic Method with Factor – Run Procedure (continued) The new optical density of the sample will be displayed. Press ↵ ↵ ↵ ↵ The change in Sample OD per minute will be displayed. Press ↵ ↵ ↵ ↵...
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Page 32: Kinetic Method With Calibrator- Run Procedure
Sherwood Scientific Limited Kinetic Method with Calibrator– Run Procedure Select Method from Main Menu using >> then ↵ ↵ ↵ ↵ Select Recall from Method Menu using >> then ↵ ↵ ↵ ↵ Select Run from Recall Menu using >> then ↵ ↵ ↵ ↵... - Page 33 Sherwood Scientific Limited Kinetic Method with Calibrator– Run Procedure (continued) The new optical density of the Standard is shown. Press ↵ ↵ ↵ ↵ The change in Optical density of the Standard is shown. Press ↵ ↵ ↵ ↵ Enter the sample number using >> then ↵ ↵ ↵ ↵ for each number Put the sample in the pre-heated water bath or heater block and press ↵...
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Page 34: Appendix A Warranty
_________________________________________________________________________________________ Warranty Term: 12 Months Sherwood Scientific Ltd (Sherwood) warrants, subject to the conditions itemised within this document, through either Sherwood personnel or personnel of its authorised distributors, to repair or replace free of all charges, including labour, any part of this product which fails within the warranty time specified above, appertaining to this particular product. - Page 35 (including but not limited to the limitations of the liability of Sherwood as set forth above), shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Ltd.
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Page 36: Appendix B Gelatin And Interference Filters
Sherwood Scientific Limited Appendix B Gelatin and Interference Filters 252 15 001 FILTER, GELATIN, 430NM 252 16 001 FILTER, GELATIN, 470NM 252 17 001 FILTER, GELATIN, 490NM 252 18 001 FILTER, GELATIN, 520NM 252 19 001 FILTER, GELATIN, 540NM 252 20 001... -
Page 37: Appendix D Time Based Methods For Clinical Analysis
Sherwood Scientific Limited Appendix D Time Based Methods for Clinical Analysis The laboratory analysis of enzymes in blood plasma is useful in the diagnosis and monitoring of various diseases especially where there is damage to tissues and organs of the body e.g. the heart, the liver and bone. Much of the raised enzyme activity,... - Page 38 Sherwood Scientific Limited Appendix D (continued) End Point Reaction If the assay is intended to measure the concentration of the substrate then excess enzyme is present and the reaction goes to completion e.g. glucose by the enzyme glucose oxidase. The substrate (Sample) mixture is placed in the cuvette. This is brought to temperature (externally in a heating device).
- Page 39 Sherwood Scientific Limited Appendix D (continued) Reaction Rate This method of analysis is used where an enzyme itself is to be measured and the substrate is added in excess to the reaction cuvette. The calculation measures the rate of change of either the initial substrate or a particular product of the reaction.
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Page 40: Appendix E Simple Factor Procedure
Sherwood Scientific Limited Appendix E “Simple” Factor Procedure Select Simple from Main Menu using >> then ↵ ↵ ↵ ↵ . Select Factor from Main Menu using >> then ↵ ↵ ↵ ↵ . Insert the filter as prompted, then ↵ ↵ ↵ ↵ . - Page 41 Sherwood Scientific Limited Appendix E (continued) “Simple” Concentration Procedure Select Simple from Main Menu using >> then ↵ ↵ ↵ ↵ . Select Concentration from Main Menu using >> then ↵ ↵ ↵ ↵ . Insert the filter as prompted then ↵ ↵ ↵ ↵ .
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