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Table of Contents
Related Manuals for epredia HM 430
Summary of Contents for epredia HM 430
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Page 3: Company Information
Epredia. This symbol is also used on the instrument, or in a All information contained in this manual is proprietary and... -
Page 4: Table Of Contents
Chapter 1 - Introduction Annual Routine Maintenance......25 Intended Purpose:...........7 Service Contract .......... 25 Description of Sliding Microtome HM 430 ..7 Chapter 6 – Cleaning and Care 26 Technical Data Sheet ........8 Chapter 2 – Operating Instructions Cleaning Intervals ......... 26 Cleaning Agents ........... -
Page 5: Safety Information
The instrument must not be used proper operation. in a manner not specified by Epredia. Correct electromagnetic environment should maintenance procedures are essential for consistent be evaluated prior to operation of the performance. -
Page 6: Chemical Safety
Chemical Safety Warranty Statement The introduction of chemicals creates potential Epredia is proud of their quality, reliability and of our hazards. Epredia has adopted the following position after-sales service. We continuously strive to with regard to the subject of volatile chemicals used improve our service to our customers. -
Page 7: Chapter 1 - Introduction
Only qualified and trained laboratory with optimal stability. personnel may operate the HM 430. The HM 430 will cut sections from 1 µm to 60 µm. The instrument may only be operated within the The specimen clamping can be moved up and... -
Page 8: Technical Data Sheet
+5°C up to +35°C Operating conditions: (at a max. rel. humidity of 60%) for indoor use only Floor loading requirements: 150 kg/m Dimensions wide: 360 mm, deep: 490 mm, high: 320 mm Weight 23 kg HM 430 Operator Guide Issue 03... -
Page 9: Chapter 2 - Operating Instructions
Should the working height be too high when setting up the microtome, unscrew the original feet and replace them with the lower ones. HM 430 Operator Guide Issue 03... -
Page 10: Locking The Sledge
• The knife sledge can be moved again. • The lock is released via a lever on the rear side of the sledge and is easily accessible from the right side behind the handle. HM 430 Operator Guide Issue 03... - Page 11 Insert the ballast (Figure 3) into the opening and close it again by using the two cover caps (Figure 4). Fix the ballast with the laterally set-screw (Figure 5) Figure 5 To remove the ballast, please execute this procedure in reverse order. HM 430 Operator Guide Issue 03...
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Page 12: Specimen Feed
10 µm to 20 µm 2 µm achieved. Approx. 3 mm of feed range is from 20 µm to 60 µm 5 µm still available. HM 430 Operator Guide Issue 03... - Page 13 • The manual feed is carried out by press the feed lever (Figure 9.2). Figure 10 Figure 9 HM 430 Operator Guide Issue 03...
- Page 14 Turning on and off the automatic position is not possible when the knife sledge is in the rear position. HM 430 Operator Guide Issue 03...
- Page 15 • Tighten the clamping lever again. • Loosen the sledge lock and move over the specimen. • When returning again over the specimen, a feed is carried out in new selected position. HM 430 Operator Guide Issue 03...
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Page 16: Specimen Clamping
• Specimens with a size up to 45 x 60 mm can be inserted. For the stability of the specimen, do not let it project over the clamping jaws too much! HM 430 Operator Guide Issue 03... -
Page 17: Changing Specimen Clamps
• Again, push the eccentric lever into the specimen block. • Then press the eccentric lever upwards to fix the specimen clamp in position. HM 430 Operator Guide Issue 03... -
Page 18: Knife Carrier
While working on the knife carrier, it Note should be locked with the locking device By experience, usable cuts are only achieved at a of the sledge (see part 2-4). clearance angle of 10° or more. HM 430 Operator Guide Issue 03... - Page 19 (size 6), the knife can be adjusted diagonally according to the tissue to be sectioned. This is called "angle cut". However, please note that due to the diagonal Figure 18 positioning the usable width of the specimen decreases! HM 430 Operator Guide Issue 03...
- Page 20 • To insert the blade, turn the clamping lever to 0 (Figure 19). • Please note that locating and clamping surfaces are clean!! • To clamp the blade, turn the clamping lever to 1. Figure 19 HM 430 Operator Guide Issue 03...
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Page 21: Standard Equipment
Standard Equipment The sliding microtome HM 430 is supplied with the following accessories: hex head wrench hex head wrench 6 mm cover rubber feet with lower height instruction manual Additional Equipment (optional) Equipment Cat. No. Disposable Blade Holder Blade holder with eccentric fast clamping •... - Page 22 Disposable blades for paraffin: • SEC 35 152200 • SEC 35e 152215 • SEC 35p 152570 Additional Accessories Fast freezing unit KS 34 S (standard specimen clamp is necessary) Para Gard (paraffin repellent), 100 ml 350170 HM 430 Operator Guide Issue 03...
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Page 23: Chapter 3 - Theory Of Operation
Chapter 3 – Theory of Operation Knife Carrier The sliding microtome HM 430 has high-precision, zero-backlash linear roller bearings (cross roller The knife carrier is easy to use and allows the bearings) for the bearing of the knife sledge and the microtome knives to be clamped and adjusted as block. -
Page 24: Chapter 4 - Working With The Microtome
Clean Knife Surfaces Trimming For optimal sectioning, front and back of the knife Take care in bringing the knife and specimen must be clean. Especially, paraffin waste must be together. removed thoroughly!! HM 430 Operator Guide Issue 03... -
Page 25: Chapter 5 - Maintenance
Service Contract Epredia offers a service contract which guarantees that your instrument is always in perfect condition. For more information, please contact the nearest Epredia sales office. -
Page 26: Chapter 6 - Cleaning And Care
• Clean the specimen clamping system, specimen commercially available paraffin repellent, e.g. Para orientation, housing and vertical carrier. Gard. Never dump the instrument in any liquid. For cleaning purposes, use a slightly moisture cleaning cloth only! HM 430 Operator Guide Issue 03... -
Page 27: Appendices
Epredia or your local, by Epredia returned to Epredia. The contact address can be authorized, dealer. found at the beginning of this instruction manual. The user must care for a clean and safe condition of the instrument when •... - Page 28 • Industrial users should contact their suppliers and observe the conditions of the contract. This product must not be disposed of together with other commercial waste. • Please contact your supplier. HM 430 Operator Guide Issue 03...
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Page 29: Index
Index Additional Ballast for Sledge ........11 General Safety............5 Additional Equipment (optional) ......... 21 Appendices ............. 27 Appendix A – Conditions for Transportation of the Instrument ............27 How to Avoid Malfunctions ........24 Adjustment of the Knife ........24 Cutting Speed ............24 Preparation of the specimen ........24 Blade Holder for Disposable Blades ...... - Page 30 Safety Information............5 Taking back the Instrument for Repair or Routine Maintenance ............27 Sectioning Instructions ..........24 Technical Data Sheet ..........8 Setting Section Thickness ........12 Theory of Operation ..........23 Setting up the Microtome ........... 9 Specimen Clamping ..........16 Specimen Clamping System and Specimen Orientation23 Specimen Coarse Feed ..........
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