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Summary of Contents for epredia HM 450
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Page 3: Company Information
This symbol is also used on the instrument, or in a and the exclusive property of Epredia and is protected by copyright. document, to indicate that irritants or potentially harmful chemicals are present. Refer to the... -
Page 4: Table Of Contents
Appendix A – Maintenance......29 Appendix B - Cleaning and Care ....29 Introduction to the HM 450 Appendix C - Transportation of the Instrument30 Description of Sliding Microtome HM 450 ..9 Revision Control This Theory of Operation ........9... -
Page 5: Emc Statement
EMC Statement This IVD equipment complies with the emissions and immunity requirements of IEC 61326-2-6. The Epredia sliding microtome HM 450 is an in vitro This equipment has been designed and tested to diagnostic device, designed to take precision CISPR 11 Class A. -
Page 6: Safety Information
The instrument must not be evaluated prior to operation of the be used in a manner not specified by Epredia. device. Good Laboratory Practice must be Correct maintenance procedures are essential for used when handling tissue samples consistent performance. -
Page 7: Chemical Safety
Chemical Safety Warranty Statement The introduction of chemicals creates potential Epredia is proud of their quality, reliability and of our hazards. Epredia has adopted the following position after-sales service. We continuously strive to with regard to the subject of volatile chemicals used improve our service to our customers. -
Page 8: Care In Using Microtome Knife
Unused knives should always be kept in a knife case. Never place the knife with the cutting edge upwards. Never try to catch a dropping knife. Never check the sharpness of the cutting edge with your fingers. The cutting edge is extremely sharp. Fig 1: Epredia Sliding Microtome HM 450 1. Blade Holder 2. Display Screen 3. -
Page 9: Introduction To The Hm 450
After changing the specimen or moving the knife or knife carrier, it is necessary to adjust the specimen The HM 450 will cut sections from 0 µm to 100 µm. to the knife edge again. This can easily be done by... -
Page 10: Working With The Microtome
Cassettes as well as different forms for injuries in case of non-compliance with round and rectangular specimens can the safety features of the HM 450 and be used for embedding. when disregarding instruction... -
Page 11: Technical Data Hm 450
Technical Data HM 450 Section thickness range 0 - 100 µm 0,5 µm for 0...5 µm 1 µm for 5...20 µm Resolution 2 µm for 20...30 µm 5 µm for 30...60 µm 10 µm for 60...100 µm Retract function for compensating thermal extension 2 µm... - Page 12 2 x T 1 ,6 AH Pollution degree Protection Class Overvoltage category Sound pressure 39 dB(A) measured with 1 m distance to the instrument Dimensions wide: 370 mm, deep: 460 mm, high: 320 mm Weight 23 kg HM 450 Operator Guide Issue 02...
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Page 13: Operating Instructions
The operator is responsible for the correct both, inform your local Epredia Representative. preparation of equipment and materials/samples for Unpack the HM 450 and remove the wrapping. Do the optimum performance of this instrument. not discard the packing, store it flat for future use. -
Page 14: Cutting Movement And Retraction
• Swivel the locking lever from the upper position Fig 4 (1) into the lower position Fig 4 (2). • For this, the red marked lever surface becomes visible to show the clamped state. HM 450 Operator Guide Issue 02... -
Page 15: Setting Section Thickness And Trimming Thickness
5 µm to 30 µm 5 µm from 30 µm to 100 µm 10 µm Fig 4-3-3 from 100 µm to 200 µm 20 µm from 200 µm to 500 µm 50 µm HM 450 Operator Guide Issue 02... -
Page 16: Specimen Feed
Specimen Feed Coarse Feed and Speed for Coarse Feed For the fast up and downward travel between specimen and knife edge, the HM 450 has a coarse feed with vertical direction. • The specimen clamping can be moved 40 mm vertically up or downwards via the two coarse feed buttons Fig 6 (1). - Page 17 Retract Function for Compensating the Thermal Extension If the HM 450 is in the manual feed mode, the normal display mode shows “Man”. Normally the ambient air slowly warms up the paraffin specimen which was cooled down before.
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Page 18: Operating Panel
• Select the desired number via the scroll button so that it is shown on the display. • Then press the reset button. • The number on the display is reset. HM 450 Operator Guide Issue 02... - Page 19 • The selected mode is permanently shown by Auto or Man in the normal display mode. HM 450 Operator Guide Issue 02...
- Page 20 Retraction turned on R:ON Retraction turned off R:OFF The following languages are available: Fig 16 • German • Chinese • English • Japanese • French • Norwegian • Spanish • Swedish • Italian • Finnish HM 450 Operator Guide Issue 02...
- Page 21 • The large display mode, Fig 22 only shows the The Service Menu must only be used active section thickness with a large number by an Epredia technical personnel. together with a symbol for the feed or trim thickness range.
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Page 22: Specimen Clamping
• Specimens with a size up to 45 x 60 mm can be inserted. For the stability of the specimen, do not let it project over the clamping jaws too much. HM 450 Operator Guide Issue 02... -
Page 23: Knife Carrier
• To replace the specimen clamp turn the The knife carrier of the HM 450 is easy to use and eccentric lever downwards, Fig 26 (1) to the stop equipped with a knife guard for user safety while and pull it off, Fig 26 (2). - Page 24 (size 6), the knife can be adjusted diagonally according to the tissue to be sectioned. This is called "angle cut". • However, please note that due to the diagonal positioning the usable width of the specimen decreases. HM 450 Operator Guide Issue 02...
- Page 25 Protection Against Injury • The knife carrier of the instrument is equipped with a knife guard which can be moved sideways. • This knife guard should be used while knife and specimen are adjusted. Fig 29 HM 450 Operator Guide Issue 02...
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Page 26: Standard Equipment
Standard Equipment Epredia Sliding Microtome HM 450 (Cat. No Height Adjustment Plates 910020) 5 mm 448070 The sliding microtome HM 450 is supplied with the following accessories: 10 mm 440400 • 1 hex head wrench 5 mm 20 mm 440410 •... - Page 27 30 cm 152260 Disposable blades for paraffin Low Profile Blades MX-35 Ultra 3053835 MX-35 Premier+ 3052835 MX-35 Premier 3051835 MB-35 Premier 3050835 MB-22 Premier 3050822 High Profile Blades HP-35 Ultra 3153735 HP-35n Ultra 3151735 HM 450 Operator Guide Issue 02...
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Page 28: Troubleshooting
Carefully select the required knife material and profile. Adjustment of the Knife Take care to adjust the proper clearance angle of the knife. Select a clearance angle adjustment of 10° or more according to the facet angle. HM 450 Operator Guide Issue 02... -
Page 29: Appendices
Cleaning Intervals Cleaning, care and decontamination of the HM 450 depends on how frequently the instrument is used. For optimal sectioning, the instrument must be free of section waste, especially the guides and clamping mechanism. -
Page 30: Appendix C - Transportation Of The Instrument30
• Remove knife or blade and store it in a safe way. some special reasons, the instrument can be • Secure the sledge via the lock of the sledge as returned to Epredia. The contact address can be described in Locking the Sledge section. - Page 31 Index Additional Equipment (Optional) ........ 26 Inserting the knife ............ 23 Disposable Blade Holder ........26 Introduction to the HM 450 ........9 Height Adjustment Plates........26 IVD Intended Purpose ..........5 Microtome Knives ..........26 Appendices............. 29 Appendix A - Maintenance ........29 Appendix B - Cleaning and Care.......
- Page 32 Selecting the Display Mode ......... 21 Technical Data HM 450 ........... 11 Service Menu ............. 21 Theory Of Operation ..........9 Speed for Coarse Feed ........20 Cutting Movement..........9 Time and Date ........... 21 Knife Carrier............9 Troubleshooting Adjustment of the Knife ........28 Cutting Speed ...........
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Page 33: Revision Control For This Document
Revision Control For This Document Date Revision Changes Made Number February 2022 IVDR compliance requirements added, including this revision record table.
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