epredia STP120 Operator's Manual

epredia STP120 Operator's Manual

Tissue processor

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Summary of Contents for epredia STP120

  • Page 3: Company Information

    This document may not, in whole or in part, be copied, photocopied, reproduced, translated or converted to any electronic or other form without prior written consent of Epredia. All information contained in this manual is proprietary and confidential, and the exclusive property of Epredia and is protected by copyright.
  • Page 4: Safety Information

    The instrument must not be used in a manner not specified by Epredia. Correct maintenance procedures are essential for consistent performance. It is recommended that users secure a maintenance contract with our service department.
  • Page 5: Symbols

    387718 Issue 16 Only use reagents recommended in the Operator Guide. • Disconnect the instrument from the mains supply before performing • maintenance. Take necessary precautions when handling glass. Wear Personal Protective • Equipment (PPE) if required. Symbols The following symbols and conventions may be used throughout this document and on the instrument: This symbol is used on the instrument, or in a document, to indicate that instructions must be followed for safe and correct operation.
  • Page 6: Chemical Safety

    Electronic Instrument (WEEE) Directive 2012/19/EU. It is marked with the following symbol: Epredia has contracts with one or more recycling / disposal companies in each EU Member State, and this product should be disposed of or recycled through them. For further...
  • Page 7: Warranty Statement

    387718 Issue 16 Warranty Statement Epredia is proud of their quality, reliability and after-sales services. We continuously strive to improve our service to our customers. Please ask your distributor or Epredia representative about service contracts which can help maintain your instrument in an optimal operating condition.
  • Page 8: Table Of Contents

    ........................... 6 HEMICAL AFETY ..........................6 NVIRONMENT ........................7 ARRANTY TATEMENT TABLE OF CONTENTS ........................8 CHAPTER 1 - INTRODUCTION TO THE EPREDIA TISSUE PROCESSOR STP120 ...... 10 :........................10 NTENDED URPOSE STP120 ............10 ESCRIPTION OF THE PREDIA ISSUE ROCESSOR .........................
  • Page 9 387718 Issue 16 CHAPTER 6 - MAINTENANCE ....................... 35 ............................. 35 LEANING ........................36 HANGING THE ILTER ....................... 37 EPLACING THE ATTERY ............................37 LARMS ........................38 ESETTING THE APPENDICES ..........................39 A - R ........................ 39 PPENDIX EAGENTS B - P ..................
  • Page 10: Chapter 1 - Introduction To The Epredia Tissue Processor Stp120

    Processor STP120 Intended Purpose: The Epredia STP120 automated tissue processor is an in vitro diagnostic device, designed to infiltrate tissue specimens with paraffin in medical, pharmaceutical research laboratories to allow for subsequent examination and diagnosis by a technologist or pathologist. Only qualified and trained laboratory personnel may operate the STP120.
  • Page 11: Technical Specifications Of The Stp 120

    387718 Issue 16 Technical Specifications of the STP 120 Sizes Diameter of the carousel 850 mm Height 500 – 700 mm Diameter of the turning circle 670 mm Weight Including packing 145 kg Net (fully equipped) 70 kg Electrical installation Rated Voltage (±10%) 100-120 V~, 50/60 Hz, 1.7A 220-240 V~, 50/60 Hz, 0.9 A...
  • Page 12 387718 Issue 16 Programming Number of programs 10 (selectable) Programmable infiltration time per from 1 s to 90 h 59 m station Rotation stirring selectable Vertical shake selectable Centrifugation selectable Centrifugation time selectable Program start delay selectable without time limit Environmental conditions at a max.
  • Page 13: Chapter 2 - Replacement And Emplacement

    During transportation the STP 120 should always be kept in an upright position. Inspect the packaging and the STP 120. If either is damaged or the contents do not match the supplied packing list, inform your local Epredia representative. Remove any support or brace holding the machine inside the packing box.
  • Page 14: Emplacement

    387718 Issue 16 Emplacement Choose a suitable flat surface measuring at least 900 mm in width x 900 m depth with 750 mm of headroom. It is absolutely necessary that the emplacement of the STP 120 has at least 750 mm of free headroom otherwise the carousel will be irrepairably damaged when it opens.
  • Page 15: Connection

    The STP120 is supplied with a power cable for connection to a standard outlet with earth. The electrical connection is located at the rear.
  • Page 16 387718 Issue 16 The display indicates (in the pre-set language), a message like the one shown here. 01/01 READY 12:50 ↑ ↓ Press the button once. Note that the carousel opens to a half-way position and the basket holder begins to turn. When the carousel stops opening, press ↑...
  • Page 17: Main Parts Of The Stp 120

    387718 Issue 16 Main Parts of the STP 120 Fig 2 – Main Parts of the STP 120 The model shown in the figure 2 corresponds to the version with two basket holders, three paraffin baths and reagent vessels. Fig 3 Under no circumstances should the maximum fill level be exceeded as the process will not be performed correctly and reagent or paraffin could be spilled.
  • Page 18: Connection Of The Paraffin Baths

    387718 Issue 16 Connection of the Paraffin Baths The paraffin baths are connected from the rear of the STP 120. Before connecting or disconnecting the paraffin baths, make sure that the STP 120 is disconnected from the power supply by turning off the main switch located at the rear of the unit.
  • Page 19: Placement Of The Baskets

    (see picture on the right). Before beginning to process tissues, you should become familiar with the handling and programming of the STP120. Design a small test program and perform some manual movements without any reagents in the vessels or samples in the baskets.
  • Page 20: Chapter 3 - Control Panel

    387718 Issue 16 Chapter 3 - Control Panel Control Panel The control panel at the front of the STP 120 contains all the keys required for handling and programing. Activate the keys by pressing in the centre of the corresponding icon or indicator.
  • Page 21 387718 Issue 16 failure), the basket will not descend into the paraffin bath (even though this is programmed) until the default safety period has elapsed. When testing the programming, skip paraffin bath positions 11 and 12 (and 10 if working with two baskets) by assigning them a time period of 00:00. If you program a time period of more than 00:00 in positions 11 and 12 (10, if working WAITING FOR PARAFFIN...
  • Page 22: Chapter 4 - Programming

    387718 Issue 16 Chapter 4 - Programming The STP 120 offers a wide range of possibilities for programming protocols for fixing and dehydrating histological samples with reagents and then infiltrating them with paraffin. The control system enables up to 10 different processes to be programmed (programs 01 to 10).
  • Page 23: Entering The Program

    387718 Issue 16 the specimen holders shakes vertically when rising and lowering. Example of a Process The table below lists Protocol Specifications: Reage Stirring rate Immersion Time Reagent vessel Hours : Minutes rpm. Program-med value Formol 01:00 Formol 01:00 Alcohol 70% 01:30 Alcohol 80% 01:30...
  • Page 24 387718 Issue 16 In this situation the screen displays the following message: If the date and time hour or language of the display are not correct, they should first be adjusted. Refer to Changing the language and Changing the date and time. 01/01 If the date, time hour and language are READY...
  • Page 25: Starting The Program

    387718 Issue 16 Temperature of the paraffin baths. Default value: 62 ˚C. • Frequency of the vertical shake (only if this option is activated by pressing the • SHAKE key when starting the program). Default value: every 10 minutes. Centrifugation time, before the change of reagent vessel. Default value: 60 seconds. •...
  • Page 26: Delayed Start

    387718 Issue 16 Delayed Start Depending on the times specified in the program for each reagent vessel, the STP 120 calculates and displays the date and time when the program will finish. If the program is to finish later than the time PROGRAM END indicated on the display, the date and time values can be altered using the UP and...
  • Page 27: End Of The Program

    387718 Issue 16 End of the Program Under normal operating conditions, the program will stop in paraffin bath 12, and issue a warning beep every five seconds. If the program being run was P 01, the screen will display the following message. Press the STOP key to stop the program.
  • Page 28: Chapter 5 - Instrument Setup

    387718 Issue 16 Chapter 5 – Instrument Setup Setup Menu For more detailed information on how to enter a program, refer to chapter Entering the program. The SETUP MENU can only be accessed if the carousel is in the stop position and no program is being run.
  • Page 29 387718 Issue 16 The SETUP MENU includes the following options and levels: Setup Menu Options Comments Changes the language used to display 1. Language messages 1.1 Spanish Y/N (YES/NO) Selects Spanish as the language 1.2 English Y/N (YES/NO) Selects English as the language 1.3 Francaise Y/N (YES/NO) Selects French as the language...
  • Page 30: Changing The Language

    387718 Issue 16 Changing the Language The carousel display can be configured to display messages in five different languages: Spanish, English, French, Italian, Dutch Step 1: Press the PROGRAM key. If the currently configured language is English, this message appears: Step 2: Press the DOWN key to go to the EDIT PROGRAM next option.
  • Page 31: Changing The Date And Time

    387718 Issue 16 Changing the Date and Time Access the SETUP MENU as indicated for changing the language until Step 5. SETUP MENU Press the DOWN key until option 3 appears 3. CLOCK on the screen. Clock. Press ENTER to select the option to change the date and time.
  • Page 32: Working With Two Baskets

    387718 Issue 16 Working with Two Baskets As an optional extra, the STP120 may be fitted with a second basket holder to double its processing capacity. When the unit is fitted with two baskets the following points should be taken into...
  • Page 33: Power Failure

    387718 Issue 16 Power Failure The STP 120 is fitted with an emergency backup battery to enable processing to continue uninterrupted or to allow manual operation to remove, if necessary, the basket with the specimen holders (refer to 'Emergency Movements'). The backup battery is designed to continue controlling the process times and enable completion of all the movements that have been started.
  • Page 34: Emergency Movements

    387718 Issue 16 Version Release date Button 1 Button 2 V. 1.3 Sept. 01 , 2001 ALARM V. 1.41 Dec. 12 , 2001 ALARM SHAKE V. 1.41 June 06 , 2002 TIMER ENTER V. 1.43 Sept. 25 , 2003 TIMER ENTER V.
  • Page 35: Chapter 6 - Maintenance

    387718 Issue 16 Chapter 6 - Maintenance Lubrication: Every six months, raise the cover of the carousel using the ↑↓ key to its uppermost position and lubricate it exclusively with the grease supplied by the manufacturer. Changing the filter: Refer to instructions on changing the filter Inspection: The STP 120 should be serviced by a manufacturer authorised technician at least once a year.
  • Page 36: Changing The Filter

    387718 Issue 16 Changing the Filter As an optional extra, the unit may be supplied with a double activated charcoal filter to help neutralise any fumes given off by the reagents used. The system controls CHANGE FILTER the life of the filter in hours. When starting the unit the following message is displayed: 0400 HOURS The value displayed (in the example 400...
  • Page 37: Replacing The Battery

    If the battery does not recharge and the alarm message does not disappear, contact your local Epredia representative immediately. Alarms Should any instrument failure occur, the STP120 displays an alarm message on the screen and the pilot light of the ALARM key begins flashing. There are two types of alarm: Alarms that can be resolved by the user •...
  • Page 38: Resetting The Unit

    387718 Issue 16 If the following alarms are activated contact your Epredia representative immediately. HOLDER LOWERED HOLDER IN CENTRE HOLDER RAISED VERTICAL FAILURE HORIZONTAL FAILURE HORIZONTAL MOTOR ON REGULATOR FAILURE HOLDER OUT OF RANGE UNIT FAILURE Resetting the Unit Proceed as described below to delete all the programs and restore the unit with the factory...
  • Page 39: Appendices

    387718 Issue 16 Appendices Appendix A - Reagents The STP 120 may be used with the reagents that are shown in the list below. Formalin, buffered or unbuffered Fixing Trinitrophenol Industrial alcohol Ethyl alcohol Dehydration Isopropyl alcohol Butyl alcohol Methanol Xylene and substitutes Trichlorethane Purification...
  • Page 40: Appendixb - Program Record Sheet

    387718 Issue 16 Appendix B - Program Record Sheet STP 120 Program Record Sheet Program number (01 to 10): Comments: Written by: Date: Reagent Time Stirring Reagent Notes vessel (HH:MM) (0/1/2) Paraffin Paraffin Comments:...
  • Page 41: Index

    Resetting the Unit ..........38 Control Panel ............ 20 Safety Information ..........4 Delayed Start............. 26 Setup Menu ............28 Description of the STP120........10 Starting the Program .......... 25 Stopping the Program ........26 Symbols ............... 5 Emergency Movements ........34 Emplacement ............
  • Page 42: Revision Control For This Document

    Revision Control For This Document Revision Date Changes made number February 2022 IVDR compliance requirements added, including this revision record table.

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