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WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL ISO-H2S-100 Microsensor for H S measurement Serial No._____________________ www.wpiinc.com 030316...
ISO-H2S-100 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Notes and Warnings ......................... 3 Parts List ............................3 Unpacking ........................... 3 OPERATING INSTRUCTIONS ......................4 Attaching the Sensor to the Microsensor Handle ............4 Polarizing the Sensor ....................... 4 Calibrating the Sensor ......................
This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—The ISO-H2S-100 sensor is a platinum wire electrode that functions similar to WPI’s ISO-H2S-2 sensor. World Precision Instruments...
INTRODUCTION The ISO-H2S-100 is a hydrogen sulfide sensor with a 100µm diameter tip. It is a dry microsensors, however, it functions like a traditional ISO-H2S-2 sensor. The sensor can be ordered in a variety of lengths from 2–5mm. It incorporates WPI’s proprietary combination electrode technology in which the hydrogen sulfide-sensing element and separate reference electrode are encased within a single shielded sensor design.
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 11 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com.
The Performance Evaluation included with your sensor shows the baseline current and the sensitivity of your sensor when it was quality tested at WPI. (In addition, it shows the polarization time of your sensor in the WPI labs.) The baseline value attainable in your lab may be slightly higher or lower, depending on the temperature* and composition of the test media.
S stock solution should be maintained at the same temperature. NOTE: For additional information, refer to the free radical analyzer manual. Most WPI manuals can be downloaded directly from www.wpiinc.com. This information can also be e-mailed when you contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com.
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Wait until the current on the display stabilizes again before continuing and record the value. This may take several minutes if the sensor has undergone a large temperature change. To the vial containing 20mL of PBS solution, sequentially inject four aliquots of the S solution (5µL, 10µL, 20µL and 40µL) into the glass vial.
If the sensor was used in a protein-rich solution, the tip should first be soaked in a protease solution for several minutes to remove protein build-up and then rinsed with distilled water. Enzymatic detergent (Enzol, WPI #7363-4) can also be used. The sensor can be sterilized chemically using an appropriate disinfectant (Cidex, WPI #7364).
The sensor has reached end of Replace the sensor. its usable life. NOTE: If you have a problem/issue with the sensor that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. World Precision Instruments...
ISO-H2S-100 SPECIFICATIONS This sensor conforms to the following specifications: Outside Tip Diameter ......................100µm Available Length ........................5mm (Length varies in 1mm increments–for example, 2mm, 3mm, 4mm and 5mm. The 5mm is standard. Sensor length is proportional to sensitivity.) Response Time ......................... 20–60sec.
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