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Summary of Contents for WPI ISO-COP-2
- Page 1 WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL ISO-OXY-2/OXELP Macrosensor for O Measurement Serial No._____________________ www.wpiinc.com 030316...
- Page 2 Other WPI Favorites Free Radical Analyzer • Real-time detection using electrochemical microsensors • Measure nitric oxide from < 0.3 nM to 100 µM. • Measure hydrogen peroxide < 10 nM to 100 mM • Measure hydrogen sulfide • Measure glucose • Measure oxygen from 0.1% to 100% •...
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Page 3: Table Of Contents
ISO-OXY-2/OXELP CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Notes and Warnings ......................... 2 Parts List ............................3 Unpacking ........................... 3 INSTRUMENT DESCRIPTION ......................4 Structure of the Sensor ......................4 OPERATING INSTRUCTIONS ......................5 Polarizing the Sensor ......................5 Calibration Procedure ...................... - Page 4 World Precision Instruments...
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Page 5: About This Manual
ISO-OXY-2/OXELP ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. -
Page 6: Introduction
Replacement membrane sleeves (pkg. of four) and filling solution can be purchased in a kit (WPI #5378). Filling solution can also be purchase separately (WPI #7326). A startup kit for this sensor is available and is recommended for the first purchase of this sensor type (WPI #5377). -
Page 7: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 15 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. -
Page 8: Instrument Description
Additional membrane sleeves are available in packages of 4 with electrolyte filling solution (WPI #5378). The start-up kit (WPI #5377) also includes replacement membrane sleeves, along with all the accessories to fill them properly with electrolyte solution. -
Page 9: Operating Instructions
NOTE: For additional sensor calibration procedures and calibration theory, refer to the free radical analyzer manual. Most WPI manuals can be downloaded directly from www.wpiinc.com. This information can also be e-mailed when you contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. - Page 10 WPI tests ISO-OXY-2 and OXELP sensors using a 0.1M PBS test media at room temperature. A sensor is tested at three known oxygen levels (0%, 21% and 100%). Known concentrations are obtained as follows: • 0% O – PBS (standard test media) is purged with 100% N •...
- Page 11 ISO-OXY-2/OXELP For 100% O concentration, insert an 18-gauge needle into the 100% vial and vigorously bubble it with 100% O gas at a constant rate for 10 minutes to saturate the solution. Drop a small stir bar into the vial with 100% O solution and place it on the magnetic stirring plate.
- Page 12 Fig. 5—Calibration output The slope of this curve indicates the sensitivity of the sensor. Once the sensitivity of the probe has been ascertained, the sensor is ready to use experimentally. World Precision Instruments...
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Page 13: Sensor Care And Maintenance
ISO-OXY-2/OXELP SENSOR CARE AND MAINTENANCE Durability and Handling The sensor is relatively durable, except for the membrane sleeve. Exercise caution when handling any sensor to avoid actions that could damage the sensor tip. Pay particular attention to the sensor membrane, because the membrane is extremely delicate and improper handling will lead to damage. -
Page 14: Sterilizing The Membrane
Dip the internal electrode 1-2cm into the ISO2 Filling Solution (WPI #7326) included in the start-up kit (WPI #5377. In the 1µA range, the current rises rapidly off scale. Then, the current value will beging to fall. Using the MicroFil™... - Page 15 (1M). If the current increases dramatically or is offscale then the membrane integrity is not good and a new membrane will have to be fitted. Additional membranes (packages of 4) with filling solution are available from WPI (#5378). World Precision Instruments...
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Page 16: Accessories
7363-4 Enzol - Enzymatic detergent (1 gal.) MF28G67-5 MicroFil electrolyte filling needle (pkg. of 5) *The start-up kit (WPI #5377) contains everything needed to begin working with this sensor, and the kit is highly recommended for first-time users (Fig. 7). The kit includes: • Calibration bottle •... -
Page 17: Troubleshooting
ISO-OXY-2/OXELP TROUBLESHOOTING Issue Possible Cause Solution The poise voltage (sensor set- Set the poise voltage to -700mV. (For ting) may be incorrectly set. the TBR, choosing the O sensor setting selects -700mV automatically.) Set the range at 10nA. The sensor may be nearing the Perform a standard calibration with at end of its usable life. -
Page 18: Specifications
NOTE: If you have a problem/issue with your sensor that falls outside the definitions of this troubleshooting section, first perform the Calibration Procedure exactly as describe on page 5 of this manual and contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. -
Page 19: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 20 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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