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Summary of Contents for WPI ISO-HPO-2
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL ISO-HPO-2 Hydrogen Peroxide Sensor for use with WPI Analyzers Serial No._____________________ www.wpiinc.com 031919...
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Page 3: Table Of Contents
ISO-HPO-2 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 Notes and Warnings ......................... 1 Parts List ............................2 Unpacking ........................... 2 INSTRUMENT DESCRIPTION ......................2 OPERATING INSTRUCTIONS ......................3 Polarizing the Sensor ....................... 3 Calibrating the Sensor ......................4 Items Required ........................ - Page 4 World Precision Instruments...
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Page 5: About This Manual
(#600012) and an internal refillable electrolyte (#100042). The sensor is designed for use in cell cultures and similar applications. The basic structure of the ISO-HPO-2 sensor is shown in Fig. 2. It consists of an internal H -sensing working/reference electrode combination. This electrode fits... -
Page 6: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 11 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. -
Page 7: Operating Instructions
(included). See “Replacing the Membrane Sleeve” on page 7. Immerse the ISO-HPO-2 sensor tip in PBS buffer solution. The sensor tip should be immersed about 0.3-0.5mm into the solution, and should not be touched by the stir bar. -
Page 8: Calibrating The Sensor
0.1M PBS buffer solution (PBS buffer tablets) (To make standard solution, see “Making of 0.1M PBS Buffer Solution” on page 5.) NOTE: The multi-port measurement chamber (WPI #NOCHM-4) can be used as an alternative calibration kit, specifically for use at different temperature condition. -
Page 9: Making Of 0.1M Pbs Buffer Solution
ISO-HPO-2 Add 231mL of 2-4% H (Sigma-Aldrich 323381) to the flask. Then, use the removed 1mL of solution from step 2 to dilute the solution in the flask to exactly 250mL again. Store this standard solution in an amber bottle, if available, or alternatively, wrap aluminum foil around the bottle to limit light intrusion. -
Page 10: Sensor Care And Maintenance
From this output, a calibration curve Fig. 4 can be created by plotting the changes in current (pA) against the changes in concentration (mM). The slope of this curve indicates sensitivity of the probe. Fig. 4—Calibration Data Interference Temperature The background current of the sensor will usually increase with increasing temperature of the experiment. -
Page 11: Storing The Sensor
Storing the Sensor When the ISO-HPO-2 sensor is not being used (for short-term storage) it should be connected to the TBR4100/1025 with power ON and with the tip suspended in 0.1M PBS solution. (Do not use water.) This will keep the sensor polarized and ready for immediate use. - Page 12 Additional membrane sleeves (WPI#600012) may be pur- chased separately. Dip the internal electrode 1–2cm into the ISO-HPO electrolyte (WPI #100042) provided in the start-up kit. The current will rise rapidly offscale. Using the Mi- croFil™ nonmetallic syringe needle (WPI #MF28G67-5) and 1mL plastic syringe (included in the Startup kit) inject approximately 100µL of electrolyte directly into...
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Page 13: Accessories
(1M). If the current increases dramatically or is offscale then the membrane integrity is not good and a new membrane will have to be fitted. Additional ISO-HPO-2 membranes (packages of 4) are available (WPI #600012). -
Page 14: Troubleshooting
See “Replacing the Membrane Sleeve” on evaporated. page 7. NOTE: If you have a problem/issue that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or technicalsupport@wpiinc.com. SPECIFICATIONS The sensor conforms to the following specifications: Outside Diameter ........................2 mm... -
Page 15: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 16 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Saarstraße 23, D-61169 Friedberg (Hesson), Germany Tel: +49 (0)6031 1602171 •...
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