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Summary of Contents for WPI ISO-COP-2
- Page 1 WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL ISO-COP-2 Macrosensor for CO Measurement Serial No._____________________ www.wpiinc.com 030316...
- Page 2 Other WPI Favorites Free Radical Analyzer • Real-time detection using electrochemical microsensors • Measure nitric oxide from < 0.3 nM to 100µM. • Measure hydrogen peroxide < 10 nM to 100mM • Measure hydrogen sulfide • Measure glucose • Measure oxygen from 0.1% to 100% •...
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Page 3: Table Of Contents
ISO-COP-2 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Sensor Design ..........................2 Notes and Warnings ......................... 2 Parts List ............................3 Unpacking ........................... 3 INSTRUMENT DESCRIPTION ......................5 Structure of the Sensor ......................5 OPERATING INSTRUCTIONS ......................6 Environmental Influences ....................... - Page 4 World Precision Instruments...
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Page 5: About This Manual
Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—ISO-COP-2 sensor World Precision Instruments... -
Page 6: Introduction
Replacement membrane sleeves can be purchased in packages of four (WPI #95620), along with filling solution (WPI #95611). A startup kit for this sensor is available and is recommended for the first purchase of this sensor type (WPI #95699). -
Page 7: Parts List
Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. The ISO-COP-2 sensor is shipped in a sealed, rigid plastic, hinged box with foam padding to avoid damage to your sensor during shipment. The tip of the sensor is protected by inserting it into a small, plastic vial containing deionized water (DIW) to keep the membrane from drying out. - Page 8 KEEP THE SENSOR STORAGE BOX and all the documentation in a safe place. Use of the sensor should begin within 30 days of receipt. Returns: Do not return any goods to WPI without obtaining prior approval (RMA # required) and instructions from WPI’s Returns Department. Goods returned (unauthorized) by collect freight may be refused.
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Page 9: Instrument Description
ISO-COP-2 INSTRUMENT DESCRIPTION Structure of the Sensor The basic structure of the ISO-COP-2 sensor is shown below (Fig. 2). Fig. 2—ISO-COP-2 Sensor assembly Gas permeable, polymeric membrane covering the end of the stainless sleeve to separate it from the external environment Disposable, protective stainless steel sleeve (WPI#95620) that houses the ... -
Page 10: Operating Instructions
The CO electrolyte filling solution (WPI #95611) must be made fresh when you are ready to use the sensor, so the ISO-COP-2 sensor is shipped with a dry membrane sleeve. Before using it, you must fill the sleeve with the CO electrolyte filling solution. -
Page 11: Polarizing The Sensor
Turn on the free radical analyzer. Set the poise voltage to 950mV. The ISO-COP-2 sensor should be allowed up to 12 hours to reach a stable base- line current of 1000-8000pA before it is used for measurement. During initial polarization the current is typically very high (well above 10nA), but within an hour it will begin to decrease rapidly. -
Page 12: Sensor Care And Maintenance
If the probe was used in a protein-rich solution, the tip should first be soaked in a protease solution for several minutes to remove protein build-up, and then in distilled water. Enzymatic detergent (for example, Enzol, WPI#7363) can also be used. World Precision Instruments... -
Page 13: Sterilizing The Membrane
Filling a single ISO-COP-2 sensor sleeve requires about 1mL. Dip the internal electrode 1-2cm into the ISO-COP-2 electrolyte (WPI #95611 pro- vided in the ISO-COP-2 start-up kit. The current will rise rapidly offscale. Using the MicroFil™ nonmetallic syringe needle (WPI #MF28G67-5) and 1mL plastic syringe (included in the Startup kit) inject approximately 100µL of freshly mixed CO elec-... - Page 14 (1M). If the current increases dramatically or is offscale then the membrane integrity is not good and a new membrane will have to be fitted. Additional ISO-COP-2 membranes (packages of 4) are available from WPI (#95620).
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Page 15: Accessories
MF28G67-5 MicroFil electrolyte filling needle (pkg. of 5) *The ISO-COP-2 start-up kit (WPI # 95699) contains everything needed to begin working with this sensor, and the kit is highly recommended for first- time ISO-COP-2 users (Fig. 4). The kit includes: •... -
Page 16: Troubleshooting
See “Replacing the Membrane Sleeve” on page 9. NOTE: If you have a problem/issue with your ISO-COP-2 sensor that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. -
Page 17: Specifications
ISO-COP-2 SPECIFICATIONS The sensor conforms to the following specifications: Outside Diameter Response Time < 10 sec Detection LImit/Range ~10nM Linear range 10nM–10µM Nominal Sensitivity (New sensor) ~0.5 pA/nM Poise Voltage 950mV Typical Quiescent Baseline Current, 25ºC 3,000pA Acceptable Baseline Range... -
Page 18: Index
INDEX Symbols 5399 11 locking cap 5 7325 3, 9, 11 7363-4 11 membrane 5 7364 9 membrane, cleaning 8 membrane sleeve, replacement 9 accessories 11 MF28G67-5 9, 11 MicroFil 9 Cidex 9 NaOH 9 durability 8 Nitric Oxide Sensors b electrical interference 6 open the package 3 electrode 5 organic solvents 2... -
Page 19: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 20 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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