Related Manuals for Buck M500
Summary of Contents for Buck M500
- Page 1 Installation and Instruction Manual M500 Scanning Infra-Red Spectrophotometer March 2015 500-9999 Buck Scientific Inc. Norwalk, CT U.S.A.
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Page 2: Table Of Contents
Page Introduction & Description Specifications Installation Preparation & Operation Parts Replacement Optical Diagram RS-232 Cable Connection Computer Wiring Diagram Windows Computer Operation of M500 14-16 M500 Instrument Control Manual 17-22 Basic & Advanced Operation 23-26 EZScan Installation EZScan Operation 28-29... - Page 3 M-500 INTRODUCTION AND DESCRIPTION Introduction: The BUCK 500 is a single beam, microprocessed, ratio recording spectrometer. Pre-sample chopping and stepper motor controlled wavelength drive give results unattainable from conventional double beam, optical nulling systems. Clean single beam design and microprocessor control, make operation simple and maintenance costs low.
- Page 4 The A-to-D and D-to-A use a single, multiplexed 12 bit D-to-A converter. The A-to-D function is achieved by successive approximation, controlled by firmware. (An automatic gain-set scan is provided at 4 times the normal fast scan speed.) The scan reset time is 17 seconds from 600 cm to 4000 cm Controls and Indicators:...
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Page 5: Specifications
SECTION B M500 SPECIFICATIONS Principle: Double beam performance via Single Beam Optics and Micro Computer Background Correction. Wavelength Range: 4000 – 600 cm Optics: A 4 position interference filter: 4000, 3060, 1980 and 980 cm F-5 aperture ratio, 0.5 Meter Monochromator with 75L/mm diffraction grating blazed @ 4 microns, pre-sample chopping at 23.6 CPS. -
Page 6: C Installation
INSTALLATION Location: The BUCK M500 should be located on a level, firm work surface free from vibration, R.F. energy sources and excessive heat. (Less than 85ºF) A clean environment will greatly increase the life of the instrument. Damage from caustic fumes, gasses or particulate matter is not under warranty. -
Page 7: D Preparation & Operation
PREPARATION AND OPERATION Preparation 1) Starting with S/N 678 the M500 uses an auto sensing power supply that will work at 100-127/200- 250 VAC. The first instruments were factory set for either 115 or 230 V.A.C., 50/60 Hz depending on customer requirements. - Page 8 7) Put the sample in the 500's beam and push “scan”. The recorder will draw the spectrum and index to the next chart. 8) Continue to run samples with one button (start/stop) operation. For the highest performance scans restore the reference run. If errors are made, push “scan” to stop the scan and the “scan reset” to index the 500 back to 4000.
- Page 9 Fast forward buttons. (#8 & #9 on page 10). 11) Put your sample into the M500 which should now be in ratio mode and push the scan button to get a synchronized scan. The pen should drop as the scan starts and rise at the end. At the end of the scan the L250 should be indexed to the next chart.
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Page 10: Parts Replacement
SECTION E PARTS REPLACEMENT Item Part No. Description 500-1037 Chopper/scan motor 500-1034 I.R. Detector 500-1053 Optical filter Set 500-0001 I.R. Source Assm. 500-1195 Power Supply 500-1119 System P.C. Assm. 500-1120 Preamp P.C. Assm. 500-1129 Ext. Recorder Cable 500-1137B Chart Paper (Linseis L250-1 Recorder) LO 120/2 &... -
Page 11: Optical Diagram
SECTION F Optical Diagram Buck M500... - Page 12 SECTION G RS-232 NULL CABLE CONNECTION BUCK 500 COMPUTER 25 Pin (Fem) 9 Pin (Fem) Connection Activity Code Connection Shield Request to Send Transmit Data Received Data 6 _____________ Clear to Send 5 ______ Data Set Ready _______8 Data Terminal Ready DTR __________________6 Recv.
- Page 13 M500 Serial and Analog Connections...
- Page 14 Windows operation. Installation: Install Buck "null-modem" cable between the 25 pin "D" (male) connector on the M500 and a 9 pin "D" (male) serial port connector on the computer. Note: not all null-modem cables have the same pin out.
- Page 15 If you use earlier versions of GRAMS/AI this version is different. Please follow these instructions carefully! Step # 1 You need your license file that Buck Scientific Inc is normally going to have in a license disk directory on the CD-ROM file. Ex: Acct123456.lic Step # 2 The program can be downloaded from http://www.gramssuite.com/downloads/grams92...
- Page 16 (It can look very confusing!) Next make sure the M500 is plugged into COM 1 or 2 (3 & 4 are not recommended) and that it has had a suitable warm up time.
- Page 17 NULL modem cable”. A diagram of the cable configuration is located in Section G page 12 at the front of this manual. Null modem cable’s standards are not all the same so use the supplied Buck cable or make your own from the diagram.
- Page 18 The cable should be plugged into the RS232 port on the back of the instrument. The instrument software communicates with the instrument using 2400-baud rate, 8 Bits, No Parity and 1 Stop Bit. Please note the number of the communications port you have plugged the cable into. When the software is first run, you will be prompted to input the communications port to which the instrument is connected.
- Page 19 GRAMS Version 9.2. The COLLECT menu will contain all the commands the user will need to set up and collect data from the BUCK Scientific Model 500 IR Instrument. There are two (2) menu options, Scan Sample and Instrument Setup.
- Page 20 During a scan the spectrum is displayed on the screen as it is being collected. Also, there is an ABORT button display on the screen. When this button is clicked the software will stop collecting data and the instrument will reset back to the starting wavenumber position and software control will be handed back to GRAMS Version 9.2 when it has reset.
- Page 21 4.2.1 Instrument Setup Ordinate Scale Ordinate Scale determines whether the spectrum is collected in Absorbance or Transmission. If this option is selected, the collected spectrum will be automatically ratioed to a background spectrum after a reference scan is saved and assigned.
- Page 22 When the software is installed a file called “BUCK500.INI” is created when you first run the BUCK 500 Instrument control software. It is stored in the same directory to which the instrument control software has been installed. This file contains RS232 communications information as well as some instrument SETUP parameters.
- Page 23 After the file has been edited, use the File/Save options from the menu bar to save the file and Exit the Notepad software. SECTION K...
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Page 24: K Basic & Advanced Operation
Operation of the BUCK M500 / GRAMS/AI Software System Basic and Advanced Operation 1. Collection of Data & Generation of Spectrum: Turn on M500 30 minutes before analysis. Turn on computer and printer. Access Start, Programs, Galactic and double click GRAMS AI. - Page 25 System will draw a BLUE line with at least 2 points on the spectrum On the bottom Spectrum, use the Right-button to ADD additional Correction points, the Left- button to move them to the desired locations. Click on “Apply” to see the result in the upper screen, click “Undo” and make any necessary ...
- Page 26 Click on "Tools", “Choose Libraries” brings up the “Library Selection – Local Library” Select desired libraries and click “OK” when done. When back to the main Spectral ID screen choose “Search” “Spectrum Search” to start it. System will complete Search routine and display the BEST MATCH in the library(ies) chosen. ...
- Page 27 12. Semi-Quantitation based on Spectral Subtraction: With the desired spectrum displayed on the screen in the ABSORBANCE mode, click on "Add-Ons" and "Spectral ID". Click on the "Tools" option at the top of the screen and “Choose Libraries…” select the desired ...
- Page 28 However, you will need to edit the buck500.ini file. Use Notepad to edit the file. Line 2 should read “Port=2” to find the M500 connected to Comm2. Remember to save the changes before you exit Notepad. (Serial Port 3 and 4 are not supported.) See EZScan Operating Instructions.
- Page 29 Do not multi-task while collecting data on EZScan. Turn on the M500 if it is not already on, and allow it to warm up for about 30 minutes. Begin EZScan by clicking on Start, Programs, EZScan and double click the EZScan Icon. If you get an error, verify the instrument is connected to comm1.
- Page 30 bring up a “Customization” box that will allow you to change the name, comments and if you wish to, the colors, depending on your printer. When you are done, click Apply, then OK. Right click and choose “Export Dialog”. The Exporting box will open and you should change destination to printer.
- Page 31 Sample Preparation Techniques for Infra-Red using the Buck M-500 Spectrometer Basic and Advanced Operation 1. For Water-free, SOLID ORGANIC powder samples = Dissolve ~100 mg of material in 1 – 2 ml of solvent (Methanol, Ethanol, Methylene Chloride, Chloroform, Acetone, Hexane or Freon-113).
- Page 32 If the sample is FLAT and can be cut, use an ATR (Attenuated Total Reflectance) accessory (Buck Models 57, 59 or PLC-11M) with a Zinc Selenide (ZnSe) or KRS-5 prism crystal. Take a background scan on the EMPTY & CLEAN crystal first, then lay the sample against the ...
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Page 33: N Sample Preparation Techniques
ACRYLATES, STYRENES, and PHTHALATE-based polymers and resins can be solvent- stripped from small fragments of a fabricated part and run as a CAST FILM or in a LIQUID CELL. Selective SOLVENT STRIPPING can be done with the following variety of solvents based on ... - Page 34 B. Did the scan motor find the home position? (If mechanically jammed it will squeal.) If the coarse and fine sensors don't find home (4000) the M500 will have to drive 360 degrees at the 3 minute speed to retry. (To save time turn the 500 on and off for a faster reset.) C.
- Page 35 In Windows 98 type C:\windows\system\regsvr32.exe -u buck500.ocx this will remove the ocx file from the registry. Then you can delete the Corbett Software diretories and install again. (In NT you don’t put the path in just regsvr32.exe /u buck500.ocx.) If this doesn’t work contant Buck for forcing the install.
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Page 36: Periodic Maintenance
SECTION P Periodic Maintenance 1. Blow the dust out of the instrument and off of the optics. If no compressed air source is available use a camel hair brush (DO NOT USE BRUSH ON GRATING!) (Cleaning of optics only recommended to be done by qualified Optical Technicians. If removed from the 500 it may need realignment). -
Page 37: Fine Calibration
They are held under tension by a # 10 locknut, washers and spring.) *This picture is for the older M500’s that have a double shafted motor with a half circle chopper blade on the right side of the wavelength drive motor. The new stronger motors use an adapter with the sensor on the left side. The chopper blade looks like a bow tie with the trailing edge of one blade half way through the sensor. -
Page 38: R Buck Product Line
SECTION R Buck Scientific Product Line Model 210VGP - Atomic Absorption Spectrophotometer - Flame and Furnace System Model 211Accusys - Atomic Absorption Spectrophotometer – Auto Gas Switching & Ignition Model 530 - Infra-Red Spectrophotometer Model 310/910 - Line of affordable Gas Chromatographs... -
Page 39: M500 Shipping List
Instructions 999-1013 Power Cord BS186-0449 .05 mm Polystyrene Film IR/FTIR Accessories/Supplies Cat 500-1052 M500 Vinyl Dust Cover DVD-IT Buck Scientific Training Video Additional For Software (Thermo Electron Corporation Pkg.) 500-1135 Null Modem Cable Grams/AI® Advanced Software Program - Optional EZ-Scan 2... -
Page 40: Policy Statement
If a detector is faulty for any reason other than a damaged window, it will be covered under warranty for one year from the ship date. We do, however, have very tight Quality Control for the M500 to prevent units from shipping with a bad detector. All failed detectors should be sent back to Buck Scientific for our inspection. - Page 41 The File section has printing and print previewing that lets you see the spectrum before you print it if needed. The files are saved in the Galactic SPC format and can open any of the M500 scan files that were done on all the versions of the Grams software packages including the Grams libraries.