Example; 20 ratio to 40, times 100 is 50 %T. Gain can be increased above 1 for high energy loss samples, but you don't want to overload the memory or resolution will be lost. In ratio mode the M530 is set to give %T or Abs. All information coming out of the RS-232 or USB plug is in raw single beam unprocessed form even when the display says ratio.
Page 4
(An automatic gain-set scan is provided at 4 times the normal fast scan speed.) The scan reset time is 17 seconds from 600 cm to 4000 cm Controls and Indicators: Display Indication: Displays wavenumber, transmittance or absorbance, scan type, scan time and gain. Controls: Gain Scan time...
SECTION B M530 SPECIFICATIONS Principle: Double beam performance via Single Beam Optics and Micro Computer Background Correction. Wavelength Range: 4000 – 600 cm Optics: A 4 position interference filter: 4000, 3045, 1980 and 960 cm F-5 aperture ratio, 0.5 Meter Monochromator with 75L/mm diffraction grating blazed @ 4 microns, pre-sample chopping at 23.6 CPS.
INSTALLATION Location: The BUCK M530 should be located on a level, firm work surface free from vibration, R.F. energy sources and excessive heat. (Less than 85ºF) A clean environment will greatly increase the life of the instrument. Damage from caustic fumes, gasses or particulate matter is not under warranty.
9) Push the “scan” button. (This will store reference data into the M530's memory). 10) After reset, change the M530 to the ratio mode and you can now get a true % T or Abs. reading. 11) Continue to run samples with one button (start/stop) operation. For the highest performance scans restore the reference run.
SECTION E PARTS REPLACEMENT Item Part No. Description 500-1037 Chopper/scan motor 500-1034 I.R. Detector 500-1053 Optical filter Set 500-0001 I.R. Source Assm. 998-1117 Power Supply 5V 998-1110 Power Supply 12V 500-1119 System P.C. Assm. 500-1120 Preamp P.C. Assm.
A dot matrix, inkjet or laser printer with its Windows driver is needed for printing spectrum. A mouse is required for the best Windows operation. Installation: Install RS232 cable between the 9 pin "D" (male) connector on the M530 and a 9 pin "D" (male) serial port connector on the computer. General: Commands issued from the "Grams/AI"...
Page 12
If you used an earlier versions of GRAMS/AI this version is different. Please follow these instructions carefully! Step # 1 You need your license file that Buck Scientific Inc is normally going to have in a license disk directory on the CD-ROM file. Ex: Acct123456.lic Step # 2 The program can be downloaded from http://www.gramssuite.com/downloads/GRAMS.asp...
Page 13
(It can look very confusing!) Next make sure the M530 is plugged into COM 1 or 2 (3 & 4 are not recommended) and that it has had a suitable warm up time.
1.0. Introduction: This manual describes how to use the BUCK Scientific Model 530 IR Instrument software with GRAMS Version 9.0. For information on using the GRAMS Version 9.0 software, please refer to the appropriate users' guide. For information on the BUCK Scientific Model 530IR Instrument...
GRAMS Version 9.0. The COLLECT menu will contain all the commands the user will need to set up and collect data from the BUCK Scientific Model 530 IR Instrument. There are two (2) menu options, Scan Sample and Instrument Setup.
Page 16
When an instrument command is selected, a new workbook page is added to the GRAMS view. It is normally Page 2. The instrument control module is added to the new workbook page and the instrument control command is automatically performed. SCAN Sample When this option is selected the software immediately collects a spectrum.
Page 17
When data collection is complete, the resulting spectrum is transferred to GRAMS view and displayed in Page 1. Software control is transferred to GRAMS and the spectrum can be saved using a different filename and spectrum comment. It is also available to be manipulated with call data commands available in GRAMS.
Page 18
This specifies the abscissa limits for acquired spectra. The range should be compatible with the operating ranges of the optical components selected. The scan range limits will be set to the maximum scan range of your instrument. The M530 has a maximum scan range of 4000 cm-1 to 600 cm-1.
Page 19
After the file has been edited, use the File/Save options from the menu bar to save the file and Exit the Notepad software.
Basic and Advanced Operation 1. Collection of Data & Generation of Spectrum: Turn on M530 30 minutes before analysis. Turn on computer and printer. Access Start, Programs, Galactic and double click GRAMS AI. Click thought the boxes until to Page 1 of GRAMS/AI.
Page 21
4. Baseline Correction: (for bad KBr pellets, uneven ATR scans) With the Zap scan on screen, click on "Applications"and choose "Baseline Correction" then click on "Multi-Point" option. System will draw a BLUE line with at least 2 points on the spectrum ...
Page 22
9. Spectral Library Search: (for users w/Spectral ID Search Module) With the desired ABS scan on screen, click on "Add-Ons" and "Spectal ID". Perform a BASIC Search by highlighting “Search”, “Spectrum Search” it will show the top 20 hits by default.
Page 23
NOTE: For routine quantitation, in the “ActiveApp” section the “IQ Predict” program module can be purchased to automatically perform these operations and prepare the calibration curve for any specific analysis. 12. Semi-Quantitation based on Spectral Subtraction: With the desired spectrum displayed on the screen in the ABSORBANCE mode, click on "Add-Ons"...
However, you will need to edit the buck500.ini file. Use Notepad to edit the file. Line 2 should read “Port=2” to find the M530 connected to Comm2. Remember to save the changes before you exit Notepad. (Serial Port 3 and 4 are not supported.) See EZScan Operating Instructions.
Do not multi-task while collecting data on EZScan. Turn on the M530 if it is not already on, and allow it to warm up for about 30 minutes. Begin EZScan by clicking on Start, Programs, EZScan and double click the EZScan Icon. If you get an error, verify the instrument is connected to comm1.
Page 26
Printing: Once you have the area on the screen you want to print, double left click. This will bring up a “Customization” box that will allow you to change the name, comments and if you wish to, the colors, depending on your printer. When you are done, click Apply, then OK. Right click and choose “Export Dialog”.
SECTION M SAMPLE PREPARATION TECHNIQUES Basic and Advanced Operation 1. For Water-free, SOLID ORGANIC powder samples = Dissolve ~100 mg of material in 1 – 2 ml of solvent (Methanol, Ethanol, Methylene Chloride, Chloroform, Acetone, Hexane or Freon-113). Using an eye-dropper, place a drop or two on a clean salt (NaCl) plate and let the solvent evaporate, leaving a CAST FILM of the sample.
Page 28
If the sample is FLAT and can be cut, use an ATR (Attenuated Total Reflectance) accessory (Buck Models 57, 59 or PLC-11M) with a Zinc Selenide (ZnSe) or KRS-5 prism crystal. Take a background scan on the EMPTY & CLEAN crystal first, then lay the sample against the crystal and squeeze down tightly without damaging the crystal.
Page 29
Do not let the evolving vapors coming out of the mouth of the tube catch fire, since this will affect the composition of the condensate that will collect in the cool part of the tube. Try to perform each pyrolysis under the same conditions (time, temperature) for best reproducibility. Don’t let pyrolysate sit on the crystal too long.
D. Detector failure? With the M530 initialized for at least a few minutes, move your fingers rapidly in and out of the light path behind the exit slits. (If good, the M530 will give some type of change on the display). If nothing, contact Buck Scientific Inc.
Page 31
In Windows 98 type C:\windows\system\regsvr32.exe -u buck500.ocx this will remove the ocx file from the registry. Then you can delete the Corbett Software diretories and install again. (In NT you don’t put the path in just regsvr32.exe /u buck500.ocx.) If this doesn’t work contant Buck for forcing the install.
1. Blow the dust out of the instrument and off of the optics. If no compressed air source is available use a camel hair brush (DO NOT USE BRUSH ON GRATING!) (Cleaning of optics only recommended to be done by qualified Optical Technicians. If removed from the M530 it may need realignment).
SECTION P FINE CALIBRATION 1. After the M530 has been on for 1 hour run a refrnc / background run and a .05 mm polystryene film run. This can be done with the EZ-Scan or Grams/AI software programs. 2. Find the actual values of the 2850.7 and 906.7 peaks. The difference should be 1944 cm...
SECTION Q BUCK SCIENTIFIC PRODUCT LINE Model 210VGP - Atomic Absorption Spectrophotometer - Flame and Furnace System Model 211Accusys - Atomic Absorption Spectrophotometer – Auto Gas Switching & Ignition Model 530 - Infra-Red Spectrophotometer Model 310/910 - Line of affordable Gas Chromatographs...
BS186-0449 .05 mm Polystyrene Film IR/FTIR Accessories/Supplies Cat 500-1052 M500 Vinyl Dust Cover DVD-IT Buck Scientific Training Video – See On Line Additional For Software (Thermo Electron Corporation Pkg.) DB9 M/F Serial Extension Cable EZ-Scan 2 Basic Software Program CD-R...
If a detector is faulty for any reason other than a damaged window, it will be covered under warranty for one year from the ship date. We do, however, have very tight Quality Control for the M530 to prevent units from shipping with a bad detector. All failed detectors should be sent back to Buck Scientific for our inspection.
Running Under Software Control The standard software program for the Model 530 is Buck Scan. It is possible to control the standalone reporting parameters from Buck Scan, however. It is important to remember that Buck Scan always receives raw data from the instrument.
Page 38
The same techniques for running blanks for cells and accessories apply to running from Buck Scan as it does for the instrument standalone. However, Buck Scan’s memory is separate and distinct from the instrument’s memory. To update Buck Scan’s memory you must make a scan in the program’s Reference mode.
Page 39
Advanced Topics for Buck Scan Scans can be restricted in range by changing the Goto and End Wavenumber settings in the program. The instrument’s scan speed and gain can be changed as well. Each one of these settings should be considered exclusive for the scans being stored.
Need help?
Do you have a question about the M530 and is the answer not in the manual?
Questions and answers